Experiment: Sucrose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20880 and EX31_RS25605 are separated by 14 nucleotides EX31_RS25605 and EX31_RS20885 are separated by 274 nucleotides EX31_RS20885 and EX31_RS20890 overlap by 4 nucleotides EX31_RS20890 and EX31_RS20895 are separated by 178 nucleotides
EX31_RS20880: EX31_RS20880 - hypothetical protein, at 821,859 to 823,301
_RS20880
EX31_RS25605: EX31_RS25605 - transposase, at 823,316 to 823,566
_RS25605
EX31_RS20885: EX31_RS20885 - conjugal transfer protein TraD, at 823,841 to 824,125
_RS20885
EX31_RS20890: EX31_RS20890 - hypothetical protein, at 824,122 to 824,379
_RS20890
EX31_RS20895: EX31_RS20895 - hypothetical protein, at 824,558 to 825,211
_RS20895
Position (kb) 824
825 Strain fitness (log2 ratio) -2
-1
0
1
2 at 823.331 kb on - strand at 823.366 kb on + strand, within EX31_RS25605 at 823.451 kb on + strand, within EX31_RS25605 at 823.483 kb on + strand, within EX31_RS25605 at 823.483 kb on + strand, within EX31_RS25605 at 823.484 kb on - strand, within EX31_RS25605 at 823.486 kb on - strand, within EX31_RS25605 at 823.488 kb on + strand, within EX31_RS25605 at 823.488 kb on + strand, within EX31_RS25605 at 823.544 kb on + strand at 823.544 kb on + strand at 823.545 kb on - strand at 823.592 kb on - strand at 823.592 kb on - strand at 823.634 kb on + strand at 823.645 kb on + strand at 823.646 kb on - strand at 823.648 kb on + strand at 823.794 kb on - strand at 823.794 kb on - strand at 823.842 kb on + strand at 823.842 kb on + strand at 823.842 kb on + strand at 823.843 kb on - strand at 823.875 kb on + strand, within EX31_RS20885 at 823.875 kb on + strand, within EX31_RS20885 at 823.875 kb on + strand, within EX31_RS20885 at 823.876 kb on - strand, within EX31_RS20885 at 823.876 kb on - strand, within EX31_RS20885 at 823.931 kb on - strand, within EX31_RS20885 at 823.934 kb on - strand, within EX31_RS20885 at 823.934 kb on - strand, within EX31_RS20885 at 823.965 kb on - strand, within EX31_RS20885 at 823.968 kb on + strand, within EX31_RS20885 at 823.969 kb on - strand, within EX31_RS20885 at 823.972 kb on + strand, within EX31_RS20885 at 823.973 kb on - strand, within EX31_RS20885 at 823.973 kb on - strand, within EX31_RS20885 at 824.074 kb on - strand, within EX31_RS20885 at 824.235 kb on + strand, within EX31_RS20890 at 824.236 kb on - strand, within EX31_RS20890 at 824.320 kb on - strand, within EX31_RS20890 at 824.423 kb on + strand at 824.438 kb on + strand at 824.524 kb on - strand at 824.592 kb on - strand at 824.611 kb on - strand at 824.611 kb on - strand at 824.725 kb on + strand, within EX31_RS20895 at 824.903 kb on + strand, within EX31_RS20895 at 824.903 kb on + strand, within EX31_RS20895 at 824.904 kb on - strand, within EX31_RS20895 at 825.102 kb on - strand, within EX31_RS20895 at 825.226 kb on - strand at 825.286 kb on - strand at 825.289 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sucrose (C) remove 823,331 - +1.9 823,366 + EX31_RS25605 0.20 -0.8 823,451 + EX31_RS25605 0.54 -1.8 823,483 + EX31_RS25605 0.67 +0.5 823,483 + EX31_RS25605 0.67 +0.5 823,484 - EX31_RS25605 0.67 -1.0 823,486 - EX31_RS25605 0.68 +0.1 823,488 + EX31_RS25605 0.69 +0.2 823,488 + EX31_RS25605 0.69 +0.5 823,544 + -1.0 823,544 + -1.8 823,545 - +1.2 823,592 - +1.0 823,592 - -0.1 823,634 + +0.2 823,645 + -0.9 823,646 - +0.5 823,648 + -0.2 823,794 - -1.1 823,794 - +0.2 823,842 + -0.8 823,842 + +0.2 823,842 + -1.3 823,843 - -0.9 823,875 + EX31_RS20885 0.12 -1.3 823,875 + EX31_RS20885 0.12 +0.2 823,875 + EX31_RS20885 0.12 -1.0 823,876 - EX31_RS20885 0.12 -0.9 823,876 - EX31_RS20885 0.12 -0.5 823,931 - EX31_RS20885 0.32 +0.2 823,934 - EX31_RS20885 0.33 +0.4 823,934 - EX31_RS20885 0.33 +0.9 823,965 - EX31_RS20885 0.44 +0.9 823,968 + EX31_RS20885 0.45 +0.7 823,969 - EX31_RS20885 0.45 +1.2 823,972 + EX31_RS20885 0.46 -1.3 823,973 - EX31_RS20885 0.46 +0.1 823,973 - EX31_RS20885 0.46 +1.7 824,074 - EX31_RS20885 0.82 -1.3 824,235 + EX31_RS20890 0.44 -1.6 824,236 - EX31_RS20890 0.44 -2.1 824,320 - EX31_RS20890 0.77 +0.8 824,423 + -0.1 824,438 + -0.1 824,524 - +1.4 824,592 - -0.1 824,611 - -1.1 824,611 - -0.3 824,725 + EX31_RS20895 0.26 -1.9 824,903 + EX31_RS20895 0.53 +1.8 824,903 + EX31_RS20895 0.53 -1.9 824,904 - EX31_RS20895 0.53 -0.6 825,102 - EX31_RS20895 0.83 +0.1 825,226 - +0.5 825,286 - +1.8 825,289 + +0.7
Or see this region's nucleotide sequence