Experiment: Sucrose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02990 and EX31_RS02995 are separated by 63 nucleotides EX31_RS02995 and EX31_RS03000 are separated by 105 nucleotides EX31_RS03000 and EX31_RS03005 overlap by 11 nucleotides
EX31_RS02990: EX31_RS02990 - acyltransferase family protein, at 82,103 to 83,068
_RS02990
EX31_RS02995: EX31_RS02995 - hypothetical protein, at 83,132 to 83,386
_RS02995
EX31_RS03000: EX31_RS03000 - hypothetical protein, at 83,492 to 83,746
_RS03000
EX31_RS03005: EX31_RS03005 - hypothetical protein, at 83,736 to 84,392
_RS03005
Position (kb) 83
84 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 82.174 kb on + strand at 82.174 kb on + strand at 82.175 kb on - strand at 82.182 kb on - strand at 82.182 kb on - strand at 82.191 kb on - strand at 82.191 kb on - strand at 82.191 kb on - strand at 82.197 kb on - strand at 82.197 kb on - strand at 82.197 kb on - strand at 82.197 kb on - strand at 82.199 kb on - strand at 82.199 kb on - strand at 82.202 kb on + strand, within EX31_RS02990 at 82.202 kb on + strand, within EX31_RS02990 at 82.202 kb on + strand, within EX31_RS02990 at 82.203 kb on - strand, within EX31_RS02990 at 82.203 kb on - strand, within EX31_RS02990 at 82.203 kb on - strand, within EX31_RS02990 at 82.210 kb on + strand, within EX31_RS02990 at 82.232 kb on - strand, within EX31_RS02990 at 82.232 kb on - strand, within EX31_RS02990 at 82.276 kb on + strand, within EX31_RS02990 at 82.276 kb on + strand, within EX31_RS02990 at 82.276 kb on + strand, within EX31_RS02990 at 82.276 kb on + strand, within EX31_RS02990 at 82.276 kb on + strand, within EX31_RS02990 at 82.276 kb on + strand, within EX31_RS02990 at 82.276 kb on + strand, within EX31_RS02990 at 82.277 kb on - strand, within EX31_RS02990 at 82.277 kb on - strand, within EX31_RS02990 at 82.277 kb on - strand, within EX31_RS02990 at 82.335 kb on + strand, within EX31_RS02990 at 82.367 kb on + strand, within EX31_RS02990 at 82.367 kb on + strand, within EX31_RS02990 at 82.367 kb on + strand, within EX31_RS02990 at 82.368 kb on - strand, within EX31_RS02990 at 82.368 kb on - strand, within EX31_RS02990 at 82.414 kb on + strand, within EX31_RS02990 at 82.414 kb on + strand, within EX31_RS02990 at 82.421 kb on - strand, within EX31_RS02990 at 82.432 kb on + strand, within EX31_RS02990 at 82.433 kb on - strand, within EX31_RS02990 at 82.436 kb on + strand, within EX31_RS02990 at 82.437 kb on - strand, within EX31_RS02990 at 82.437 kb on - strand, within EX31_RS02990 at 82.526 kb on + strand, within EX31_RS02990 at 82.526 kb on + strand, within EX31_RS02990 at 82.544 kb on + strand, within EX31_RS02990 at 82.545 kb on - strand, within EX31_RS02990 at 82.545 kb on - strand, within EX31_RS02990 at 82.558 kb on + strand, within EX31_RS02990 at 82.559 kb on - strand, within EX31_RS02990 at 82.559 kb on - strand, within EX31_RS02990 at 82.559 kb on - strand, within EX31_RS02990 at 82.602 kb on + strand, within EX31_RS02990 at 82.603 kb on - strand, within EX31_RS02990 at 82.607 kb on + strand, within EX31_RS02990 at 82.608 kb on - strand, within EX31_RS02990 at 82.610 kb on + strand, within EX31_RS02990 at 82.610 kb on + strand, within EX31_RS02990 at 82.610 kb on + strand, within EX31_RS02990 at 82.630 kb on - strand, within EX31_RS02990 at 82.630 kb on - strand, within EX31_RS02990 at 82.630 kb on - strand, within EX31_RS02990 at 82.631 kb on + strand, within EX31_RS02990 at 82.631 kb on + strand, within EX31_RS02990 at 82.631 kb on + strand, within EX31_RS02990 at 82.631 kb on + strand, within EX31_RS02990 at 82.632 kb on - strand, within EX31_RS02990 at 82.656 kb on - strand, within EX31_RS02990 at 82.775 kb on - strand, within EX31_RS02990 at 82.796 kb on + strand, within EX31_RS02990 at 82.796 kb on + strand, within EX31_RS02990 at 82.796 kb on + strand, within EX31_RS02990 at 82.796 kb on + strand, within EX31_RS02990 at 82.796 kb on + strand, within EX31_RS02990 at 82.797 kb on - strand, within EX31_RS02990 at 82.797 kb on - strand, within EX31_RS02990 at 82.797 kb on - strand, within EX31_RS02990 at 82.797 kb on - strand, within EX31_RS02990 at 82.797 kb on - strand, within EX31_RS02990 at 82.797 kb on - strand, within EX31_RS02990 at 82.797 kb on - strand, within EX31_RS02990 at 82.797 kb on - strand, within EX31_RS02990 at 82.797 kb on - strand, within EX31_RS02990 at 82.798 kb on + strand, within EX31_RS02990 at 82.799 kb on - strand, within EX31_RS02990 at 82.799 kb on - strand, within EX31_RS02990 at 82.799 kb on - strand, within EX31_RS02990 at 82.809 kb on + strand, within EX31_RS02990 at 82.810 kb on - strand, within EX31_RS02990 at 82.810 kb on - strand, within EX31_RS02990 at 82.810 kb on - strand, within EX31_RS02990 at 82.810 kb on - strand, within EX31_RS02990 at 82.820 kb on - strand, within EX31_RS02990 at 82.908 kb on - strand, within EX31_RS02990 at 82.909 kb on + strand, within EX31_RS02990 at 82.910 kb on - strand, within EX31_RS02990 at 82.936 kb on - strand, within EX31_RS02990 at 82.936 kb on - strand, within EX31_RS02990 at 82.936 kb on - strand, within EX31_RS02990 at 82.936 kb on - strand, within EX31_RS02990 at 83.038 kb on + strand at 83.059 kb on + strand at 83.096 kb on - strand at 83.134 kb on - strand at 83.205 kb on - strand, within EX31_RS02995 at 83.249 kb on - strand, within EX31_RS02995 at 83.259 kb on - strand, within EX31_RS02995 at 83.277 kb on - strand, within EX31_RS02995 at 83.312 kb on + strand, within EX31_RS02995 at 83.313 kb on - strand, within EX31_RS02995 at 83.313 kb on - strand, within EX31_RS02995 at 83.315 kb on - strand, within EX31_RS02995 at 83.315 kb on - strand, within EX31_RS02995 at 83.426 kb on + strand at 83.427 kb on - strand at 83.430 kb on + strand at 83.430 kb on + strand at 83.431 kb on - strand at 83.441 kb on + strand at 83.446 kb on - strand at 83.446 kb on - strand at 83.448 kb on - strand at 83.448 kb on - strand at 83.448 kb on - strand at 83.448 kb on - strand at 83.474 kb on + strand at 83.475 kb on - strand at 83.497 kb on + strand at 83.507 kb on - strand at 83.507 kb on - strand at 83.524 kb on + strand, within EX31_RS03000 at 83.536 kb on + strand, within EX31_RS03000 at 83.609 kb on - strand, within EX31_RS03000 at 83.663 kb on - strand, within EX31_RS03000 at 83.669 kb on + strand, within EX31_RS03000 at 83.670 kb on - strand, within EX31_RS03000 at 83.680 kb on + strand, within EX31_RS03000 at 83.680 kb on + strand, within EX31_RS03000 at 83.680 kb on + strand, within EX31_RS03000 at 83.681 kb on - strand, within EX31_RS03000 at 83.681 kb on - strand, within EX31_RS03000 at 83.684 kb on + strand, within EX31_RS03000 at 83.684 kb on + strand, within EX31_RS03000 at 83.685 kb on - strand, within EX31_RS03000 at 83.685 kb on - strand, within EX31_RS03000 at 83.685 kb on - strand, within EX31_RS03000 at 83.685 kb on - strand, within EX31_RS03000 at 83.690 kb on - strand, within EX31_RS03000 at 83.690 kb on - strand, within EX31_RS03000 at 83.690 kb on - strand, within EX31_RS03000 at 83.690 kb on - strand, within EX31_RS03000 at 83.690 kb on - strand, within EX31_RS03000 at 83.701 kb on - strand, within EX31_RS03000 at 83.701 kb on - strand, within EX31_RS03000 at 83.726 kb on - strand at 83.726 kb on - strand at 83.738 kb on - strand at 83.814 kb on + strand, within EX31_RS03005 at 83.814 kb on + strand, within EX31_RS03005 at 83.814 kb on + strand, within EX31_RS03005 at 83.814 kb on + strand, within EX31_RS03005 at 83.814 kb on + strand, within EX31_RS03005 at 83.815 kb on - strand, within EX31_RS03005 at 83.835 kb on + strand, within EX31_RS03005 at 83.835 kb on + strand, within EX31_RS03005 at 83.836 kb on - strand, within EX31_RS03005 at 83.836 kb on - strand, within EX31_RS03005 at 83.836 kb on - strand, within EX31_RS03005 at 83.836 kb on - strand, within EX31_RS03005 at 83.836 kb on - strand, within EX31_RS03005 at 83.857 kb on + strand, within EX31_RS03005 at 83.859 kb on + strand, within EX31_RS03005 at 83.860 kb on - strand, within EX31_RS03005 at 83.870 kb on - strand, within EX31_RS03005 at 83.894 kb on - strand, within EX31_RS03005 at 83.958 kb on + strand, within EX31_RS03005 at 83.958 kb on + strand, within EX31_RS03005 at 83.958 kb on + strand, within EX31_RS03005 at 83.958 kb on + strand, within EX31_RS03005 at 83.959 kb on - strand, within EX31_RS03005 at 83.959 kb on - strand, within EX31_RS03005 at 84.031 kb on - strand, within EX31_RS03005 at 84.033 kb on + strand, within EX31_RS03005 at 84.045 kb on + strand, within EX31_RS03005 at 84.045 kb on + strand, within EX31_RS03005 at 84.060 kb on + strand, within EX31_RS03005 at 84.060 kb on + strand, within EX31_RS03005 at 84.093 kb on - strand, within EX31_RS03005 at 84.179 kb on + strand, within EX31_RS03005 at 84.180 kb on - strand, within EX31_RS03005 at 84.226 kb on - strand, within EX31_RS03005 at 84.226 kb on - strand, within EX31_RS03005 at 84.226 kb on - strand, within EX31_RS03005 at 84.243 kb on + strand, within EX31_RS03005 at 84.243 kb on + strand, within EX31_RS03005 at 84.243 kb on + strand, within EX31_RS03005 at 84.243 kb on + strand, within EX31_RS03005 at 84.243 kb on + strand, within EX31_RS03005 at 84.243 kb on + strand, within EX31_RS03005 at 84.243 kb on + strand, within EX31_RS03005 at 84.243 kb on + strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.247 kb on + strand, within EX31_RS03005 at 84.247 kb on + strand, within EX31_RS03005 at 84.248 kb on - strand, within EX31_RS03005 at 84.248 kb on - strand, within EX31_RS03005 at 84.248 kb on - strand, within EX31_RS03005 at 84.248 kb on - strand, within EX31_RS03005 at 84.248 kb on - strand, within EX31_RS03005 at 84.274 kb on + strand, within EX31_RS03005 at 84.274 kb on + strand, within EX31_RS03005 at 84.275 kb on - strand, within EX31_RS03005 at 84.275 kb on - strand, within EX31_RS03005 at 84.328 kb on + strand at 84.330 kb on + strand at 84.330 kb on + strand at 84.331 kb on - strand at 84.331 kb on - strand at 84.331 kb on - strand at 84.331 kb on - strand at 84.331 kb on - strand at 84.331 kb on - strand at 84.351 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sucrose (C) remove 82,174 + -1.0 82,174 + +0.6 82,175 - +0.6 82,182 - -0.0 82,182 - +0.9 82,191 - +1.8 82,191 - +1.3 82,191 - -0.0 82,197 - +0.4 82,197 - +1.9 82,197 - +0.9 82,197 - +1.0 82,199 - +0.3 82,199 - +0.8 82,202 + EX31_RS02990 0.10 +0.3 82,202 + EX31_RS02990 0.10 -0.0 82,202 + EX31_RS02990 0.10 +0.3 82,203 - EX31_RS02990 0.10 +1.0 82,203 - EX31_RS02990 0.10 -0.5 82,203 - EX31_RS02990 0.10 +0.1 82,210 + EX31_RS02990 0.11 +0.8 82,232 - EX31_RS02990 0.13 -1.6 82,232 - EX31_RS02990 0.13 -0.1 82,276 + EX31_RS02990 0.18 -0.1 82,276 + EX31_RS02990 0.18 -0.7 82,276 + EX31_RS02990 0.18 +0.8 82,276 + EX31_RS02990 0.18 +1.0 82,276 + EX31_RS02990 0.18 +2.3 82,276 + EX31_RS02990 0.18 -0.2 82,276 + EX31_RS02990 0.18 +0.6 82,277 - EX31_RS02990 0.18 +0.4 82,277 - EX31_RS02990 0.18 +1.2 82,277 - EX31_RS02990 0.18 -0.7 82,335 + EX31_RS02990 0.24 +0.6 82,367 + EX31_RS02990 0.27 +0.1 82,367 + EX31_RS02990 0.27 +0.4 82,367 + EX31_RS02990 0.27 +0.6 82,368 - EX31_RS02990 0.27 +0.1 82,368 - EX31_RS02990 0.27 +1.7 82,414 + EX31_RS02990 0.32 +0.3 82,414 + EX31_RS02990 0.32 +0.4 82,421 - EX31_RS02990 0.33 +0.2 82,432 + EX31_RS02990 0.34 -0.1 82,433 - EX31_RS02990 0.34 -0.7 82,436 + EX31_RS02990 0.34 -0.3 82,437 - EX31_RS02990 0.35 +1.0 82,437 - EX31_RS02990 0.35 -0.1 82,526 + EX31_RS02990 0.44 +0.3 82,526 + EX31_RS02990 0.44 -0.7 82,544 + EX31_RS02990 0.46 -0.1 82,545 - EX31_RS02990 0.46 +0.2 82,545 - EX31_RS02990 0.46 -0.8 82,558 + EX31_RS02990 0.47 -0.8 82,559 - EX31_RS02990 0.47 -1.3 82,559 - EX31_RS02990 0.47 +1.1 82,559 - EX31_RS02990 0.47 -0.7 82,602 + EX31_RS02990 0.52 +0.8 82,603 - EX31_RS02990 0.52 -0.1 82,607 + EX31_RS02990 0.52 -2.9 82,608 - EX31_RS02990 0.52 +3.3 82,610 + EX31_RS02990 0.52 +0.1 82,610 + EX31_RS02990 0.52 -1.5 82,610 + EX31_RS02990 0.52 +1.3 82,630 - EX31_RS02990 0.55 +0.0 82,630 - EX31_RS02990 0.55 +0.3 82,630 - EX31_RS02990 0.55 +1.3 82,631 + EX31_RS02990 0.55 -0.4 82,631 + EX31_RS02990 0.55 +0.3 82,631 + EX31_RS02990 0.55 +1.3 82,631 + EX31_RS02990 0.55 +1.9 82,632 - EX31_RS02990 0.55 -0.7 82,656 - EX31_RS02990 0.57 +2.0 82,775 - EX31_RS02990 0.70 -1.4 82,796 + EX31_RS02990 0.72 +0.2 82,796 + EX31_RS02990 0.72 -0.3 82,796 + EX31_RS02990 0.72 +0.3 82,796 + EX31_RS02990 0.72 -0.8 82,796 + EX31_RS02990 0.72 +2.3 82,797 - EX31_RS02990 0.72 +0.3 82,797 - EX31_RS02990 0.72 +0.2 82,797 - EX31_RS02990 0.72 +0.1 82,797 - EX31_RS02990 0.72 -0.7 82,797 - EX31_RS02990 0.72 +0.7 82,797 - EX31_RS02990 0.72 +1.0 82,797 - EX31_RS02990 0.72 +0.1 82,797 - EX31_RS02990 0.72 -1.7 82,797 - EX31_RS02990 0.72 +0.7 82,798 + EX31_RS02990 0.72 -1.3 82,799 - EX31_RS02990 0.72 +1.4 82,799 - EX31_RS02990 0.72 +1.4 82,799 - EX31_RS02990 0.72 -1.0 82,809 + EX31_RS02990 0.73 -2.4 82,810 - EX31_RS02990 0.73 +1.1 82,810 - EX31_RS02990 0.73 -1.5 82,810 - EX31_RS02990 0.73 -0.5 82,810 - EX31_RS02990 0.73 -0.3 82,820 - EX31_RS02990 0.74 -0.4 82,908 - EX31_RS02990 0.83 -0.1 82,909 + EX31_RS02990 0.83 +0.9 82,910 - EX31_RS02990 0.84 -0.3 82,936 - EX31_RS02990 0.86 -1.0 82,936 - EX31_RS02990 0.86 -1.2 82,936 - EX31_RS02990 0.86 -0.7 82,936 - EX31_RS02990 0.86 -1.8 83,038 + -0.5 83,059 + +0.1 83,096 - +1.0 83,134 - -0.5 83,205 - EX31_RS02995 0.29 -0.3 83,249 - EX31_RS02995 0.46 -0.7 83,259 - EX31_RS02995 0.50 -0.1 83,277 - EX31_RS02995 0.57 -2.7 83,312 + EX31_RS02995 0.71 -0.7 83,313 - EX31_RS02995 0.71 -1.9 83,313 - EX31_RS02995 0.71 -0.4 83,315 - EX31_RS02995 0.72 -1.1 83,315 - EX31_RS02995 0.72 -1.5 83,426 + +0.3 83,427 - -0.2 83,430 + -0.3 83,430 + -0.1 83,431 - +0.1 83,441 + -0.3 83,446 - +0.0 83,446 - -1.9 83,448 - +0.5 83,448 - +0.7 83,448 - -0.2 83,448 - +1.3 83,474 + +1.1 83,475 - +0.8 83,497 + +0.7 83,507 - +0.6 83,507 - -0.4 83,524 + EX31_RS03000 0.13 -0.7 83,536 + EX31_RS03000 0.17 -0.4 83,609 - EX31_RS03000 0.46 +0.6 83,663 - EX31_RS03000 0.67 -0.1 83,669 + EX31_RS03000 0.69 +0.6 83,670 - EX31_RS03000 0.70 -0.8 83,680 + EX31_RS03000 0.74 +1.1 83,680 + EX31_RS03000 0.74 -0.3 83,680 + EX31_RS03000 0.74 +0.2 83,681 - EX31_RS03000 0.74 +0.1 83,681 - EX31_RS03000 0.74 +0.7 83,684 + EX31_RS03000 0.75 -1.5 83,684 + EX31_RS03000 0.75 -2.1 83,685 - EX31_RS03000 0.76 +0.9 83,685 - EX31_RS03000 0.76 +0.8 83,685 - EX31_RS03000 0.76 -0.7 83,685 - EX31_RS03000 0.76 +0.3 83,690 - EX31_RS03000 0.78 -0.7 83,690 - EX31_RS03000 0.78 +0.2 83,690 - EX31_RS03000 0.78 +0.1 83,690 - EX31_RS03000 0.78 +2.2 83,690 - EX31_RS03000 0.78 -0.5 83,701 - EX31_RS03000 0.82 +0.5 83,701 - EX31_RS03000 0.82 +1.6 83,726 - -0.7 83,726 - -0.3 83,738 - +0.6 83,814 + EX31_RS03005 0.12 +0.1 83,814 + EX31_RS03005 0.12 +0.3 83,814 + EX31_RS03005 0.12 +1.3 83,814 + EX31_RS03005 0.12 +0.1 83,814 + EX31_RS03005 0.12 +0.5 83,815 - EX31_RS03005 0.12 -2.0 83,835 + EX31_RS03005 0.15 +0.8 83,835 + EX31_RS03005 0.15 -1.0 83,836 - EX31_RS03005 0.15 +1.3 83,836 - EX31_RS03005 0.15 +1.6 83,836 - EX31_RS03005 0.15 -0.1 83,836 - EX31_RS03005 0.15 +0.7 83,836 - EX31_RS03005 0.15 +0.9 83,857 + EX31_RS03005 0.18 +0.9 83,859 + EX31_RS03005 0.19 +0.3 83,860 - EX31_RS03005 0.19 -0.6 83,870 - EX31_RS03005 0.20 -1.0 83,894 - EX31_RS03005 0.24 +2.0 83,958 + EX31_RS03005 0.34 +0.4 83,958 + EX31_RS03005 0.34 +1.3 83,958 + EX31_RS03005 0.34 +0.9 83,958 + EX31_RS03005 0.34 +0.6 83,959 - EX31_RS03005 0.34 +2.3 83,959 - EX31_RS03005 0.34 -0.3 84,031 - EX31_RS03005 0.45 +1.9 84,033 + EX31_RS03005 0.45 -0.3 84,045 + EX31_RS03005 0.47 -1.1 84,045 + EX31_RS03005 0.47 +0.9 84,060 + EX31_RS03005 0.49 +1.3 84,060 + EX31_RS03005 0.49 +0.3 84,093 - EX31_RS03005 0.54 +0.6 84,179 + EX31_RS03005 0.67 +0.7 84,180 - EX31_RS03005 0.68 +0.1 84,226 - EX31_RS03005 0.75 +0.3 84,226 - EX31_RS03005 0.75 +0.6 84,226 - EX31_RS03005 0.75 -1.0 84,243 + EX31_RS03005 0.77 -1.7 84,243 + EX31_RS03005 0.77 +2.3 84,243 + EX31_RS03005 0.77 -0.1 84,243 + EX31_RS03005 0.77 -2.3 84,243 + EX31_RS03005 0.77 +0.7 84,243 + EX31_RS03005 0.77 -1.4 84,243 + EX31_RS03005 0.77 -0.9 84,243 + EX31_RS03005 0.77 -0.3 84,244 - EX31_RS03005 0.77 -1.7 84,244 - EX31_RS03005 0.77 -1.5 84,244 - EX31_RS03005 0.77 +1.9 84,244 - EX31_RS03005 0.77 +1.5 84,244 - EX31_RS03005 0.77 +0.1 84,244 - EX31_RS03005 0.77 +1.3 84,244 - EX31_RS03005 0.77 +0.3 84,244 - EX31_RS03005 0.77 +0.1 84,244 - EX31_RS03005 0.77 -0.2 84,244 - EX31_RS03005 0.77 +1.9 84,247 + EX31_RS03005 0.78 -0.5 84,247 + EX31_RS03005 0.78 -0.3 84,248 - EX31_RS03005 0.78 +0.3 84,248 - EX31_RS03005 0.78 +0.2 84,248 - EX31_RS03005 0.78 +1.0 84,248 - EX31_RS03005 0.78 -1.0 84,248 - EX31_RS03005 0.78 +0.5 84,274 + EX31_RS03005 0.82 +1.0 84,274 + EX31_RS03005 0.82 +0.7 84,275 - EX31_RS03005 0.82 +0.3 84,275 - EX31_RS03005 0.82 +0.5 84,328 + +1.0 84,330 + +1.7 84,330 + -0.4 84,331 - +0.8 84,331 - +0.6 84,331 - +1.0 84,331 - +1.4 84,331 - +0.3 84,331 - -0.9 84,351 - -1.0
Or see this region's nucleotide sequence