Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20190 and CA265_RS20195 are separated by 255 nucleotides CA265_RS20195 and CA265_RS20200 are separated by 177 nucleotides CA265_RS20200 and CA265_RS20205 are separated by 250 nucleotides CA265_RS20205 and CA265_RS20210 are separated by 50 nucleotides
CA265_RS20190: CA265_RS20190 - thiamine biosynthesis protein ThiF, at 4,990,061 to 4,991,098
_RS20190
CA265_RS20195: CA265_RS20195 - hypothetical protein, at 4,991,354 to 4,991,905
_RS20195
CA265_RS20200: CA265_RS20200 - nuclease, at 4,992,083 to 4,992,595
_RS20200
CA265_RS20205: CA265_RS20205 - very short patch repair endonuclease, at 4,992,846 to 4,993,349
_RS20205
CA265_RS20210: CA265_RS20210 - hypothetical protein, at 4,993,400 to 4,994,074
_RS20210
Position (kb)
4992
4993 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4991.193 kb on - strand at 4991.326 kb on - strand at 4991.344 kb on + strand at 4991.348 kb on + strand at 4991.393 kb on - strand at 4991.519 kb on - strand, within CA265_RS20195 at 4991.540 kb on + strand, within CA265_RS20195 at 4991.561 kb on - strand, within CA265_RS20195 at 4991.561 kb on - strand, within CA265_RS20195 at 4991.836 kb on + strand, within CA265_RS20195 at 4991.864 kb on + strand at 4991.909 kb on + strand at 4991.988 kb on - strand at 4991.991 kb on - strand at 4991.995 kb on + strand at 4992.077 kb on - strand at 4992.077 kb on - strand at 4992.078 kb on + strand at 4992.222 kb on + strand, within CA265_RS20200 at 4992.351 kb on + strand, within CA265_RS20200 at 4992.352 kb on - strand, within CA265_RS20200 at 4992.384 kb on + strand, within CA265_RS20200 at 4992.407 kb on - strand, within CA265_RS20200 at 4992.598 kb on + strand at 4992.606 kb on + strand at 4992.611 kb on + strand at 4992.615 kb on + strand at 4992.616 kb on - strand at 4992.656 kb on + strand at 4992.657 kb on - strand at 4992.682 kb on - strand at 4992.691 kb on - strand at 4992.691 kb on - strand at 4992.802 kb on - strand at 4992.840 kb on + strand at 4992.845 kb on + strand at 4992.919 kb on - strand, within CA265_RS20205 at 4992.924 kb on - strand, within CA265_RS20205 at 4992.928 kb on + strand, within CA265_RS20205 at 4992.928 kb on + strand, within CA265_RS20205 at 4992.957 kb on - strand, within CA265_RS20205 at 4992.989 kb on - strand, within CA265_RS20205 at 4993.028 kb on - strand, within CA265_RS20205 at 4993.058 kb on + strand, within CA265_RS20205 at 4993.070 kb on + strand, within CA265_RS20205 at 4993.070 kb on + strand, within CA265_RS20205 at 4993.070 kb on + strand, within CA265_RS20205 at 4993.071 kb on - strand, within CA265_RS20205 at 4993.071 kb on - strand, within CA265_RS20205 at 4993.188 kb on - strand, within CA265_RS20205 at 4993.233 kb on + strand, within CA265_RS20205 at 4993.236 kb on - strand, within CA265_RS20205 at 4993.303 kb on + strand at 4993.306 kb on - strand at 4993.310 kb on - strand at 4993.343 kb on + strand at 4993.344 kb on - strand at 4993.347 kb on + strand at 4993.347 kb on + strand at 4993.348 kb on - strand at 4993.373 kb on - strand at 4993.411 kb on - strand at 4993.412 kb on + strand at 4993.413 kb on - strand at 4993.415 kb on - strand at 4993.417 kb on - strand at 4993.454 kb on + strand at 4993.455 kb on - strand at 4993.525 kb on + strand, within CA265_RS20210 at 4993.530 kb on - strand, within CA265_RS20210 at 4993.552 kb on + strand, within CA265_RS20210
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 4,991,193 - +1.2 4,991,326 - -3.2 4,991,344 + +1.0 4,991,348 + +0.6 4,991,393 - -0.3 4,991,519 - CA265_RS20195 0.30 -0.7 4,991,540 + CA265_RS20195 0.34 +0.6 4,991,561 - CA265_RS20195 0.38 -1.9 4,991,561 - CA265_RS20195 0.38 -0.7 4,991,836 + CA265_RS20195 0.87 -0.7 4,991,864 + +0.0 4,991,909 + -1.2 4,991,988 - -1.0 4,991,991 - +2.1 4,991,995 + +0.3 4,992,077 - +0.9 4,992,077 - -1.9 4,992,078 + +0.7 4,992,222 + CA265_RS20200 0.27 +0.1 4,992,351 + CA265_RS20200 0.52 -0.5 4,992,352 - CA265_RS20200 0.52 -2.5 4,992,384 + CA265_RS20200 0.59 -1.9 4,992,407 - CA265_RS20200 0.63 -0.2 4,992,598 + -0.3 4,992,606 + -0.7 4,992,611 + +0.6 4,992,615 + -0.0 4,992,616 - -0.7 4,992,656 + -0.1 4,992,657 - +1.5 4,992,682 - +0.6 4,992,691 - +0.9 4,992,691 - -1.6 4,992,802 - -1.1 4,992,840 + -0.6 4,992,845 + -1.2 4,992,919 - CA265_RS20205 0.14 -0.1 4,992,924 - CA265_RS20205 0.15 +0.9 4,992,928 + CA265_RS20205 0.16 -1.1 4,992,928 + CA265_RS20205 0.16 -0.6 4,992,957 - CA265_RS20205 0.22 -1.9 4,992,989 - CA265_RS20205 0.28 +0.0 4,993,028 - CA265_RS20205 0.36 +0.8 4,993,058 + CA265_RS20205 0.42 -0.3 4,993,070 + CA265_RS20205 0.44 +0.3 4,993,070 + CA265_RS20205 0.44 +0.5 4,993,070 + CA265_RS20205 0.44 +0.1 4,993,071 - CA265_RS20205 0.45 +1.2 4,993,071 - CA265_RS20205 0.45 -0.0 4,993,188 - CA265_RS20205 0.68 -0.5 4,993,233 + CA265_RS20205 0.77 -0.1 4,993,236 - CA265_RS20205 0.77 +0.8 4,993,303 + +0.8 4,993,306 - +0.3 4,993,310 - +0.5 4,993,343 + -0.1 4,993,344 - +1.0 4,993,347 + +1.0 4,993,347 + +0.6 4,993,348 - +0.6 4,993,373 - +0.1 4,993,411 - -0.2 4,993,412 + -0.1 4,993,413 - +0.2 4,993,415 - +0.6 4,993,417 - -0.1 4,993,454 + +0.7 4,993,455 - -0.0 4,993,525 + CA265_RS20210 0.19 -0.6 4,993,530 - CA265_RS20210 0.19 -0.2 4,993,552 + CA265_RS20210 0.23 -0.5
Or see this region's nucleotide sequence