Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32535 and BPHYT_RS32540 are separated by 187 nucleotides BPHYT_RS32540 and BPHYT_RS32545 are separated by 152 nucleotides BPHYT_RS32545 and BPHYT_RS32550 are separated by 1 nucleotides BPHYT_RS32550 and BPHYT_RS32555 overlap by 4 nucleotides
BPHYT_RS32535: BPHYT_RS32535 - transposase, at 2,871,526 to 2,871,876
_RS32535
BPHYT_RS32540: BPHYT_RS32540 - hypothetical protein, at 2,872,064 to 2,872,321
_RS32540
BPHYT_RS32545: BPHYT_RS32545 - hypothetical protein, at 2,872,474 to 2,872,689
_RS32545
BPHYT_RS32550: BPHYT_RS32550 - general secretion pathway protein A, at 2,872,691 to 2,873,548
_RS32550
BPHYT_RS32555: BPHYT_RS32555 - integrase, at 2,873,545 to 2,874,981
_RS32555
Position (kb) 2872
2873
2874 Strain fitness (log2 ratio) -1
0
1 at 2871.746 kb on + strand, within BPHYT_RS32535 at 2871.845 kb on + strand at 2871.845 kb on + strand at 2871.846 kb on - strand at 2871.846 kb on - strand at 2872.082 kb on + strand at 2872.267 kb on - strand, within BPHYT_RS32540 at 2872.370 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 2,871,746 + BPHYT_RS32535 0.63 +0.8 2,871,845 + -0.1 2,871,845 + +0.0 2,871,846 - +0.2 2,871,846 - -0.5 2,872,082 + +0.2 2,872,267 - BPHYT_RS32540 0.79 +0.4 2,872,370 + -0.4
Or see this region's nucleotide sequence