Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13740 and BPHYT_RS13745 overlap by 4 nucleotides BPHYT_RS13745 and BPHYT_RS13750 are separated by 54 nucleotides BPHYT_RS13750 and BPHYT_RS13755 are separated by 50 nucleotides BPHYT_RS13755 and BPHYT_RS13760 are separated by 134 nucleotides
BPHYT_RS13740: BPHYT_RS13740 - NAD(P)H quinone oxidoreductase, at 3,131,410 to 3,132,012
_RS13740
BPHYT_RS13745: BPHYT_RS13745 - membrane protein, at 3,132,009 to 3,132,545
_RS13745
BPHYT_RS13750: BPHYT_RS13750 - D-2-hydroxyacid dehydrogenase, at 3,132,600 to 3,134,018
_RS13750
BPHYT_RS13755: BPHYT_RS13755 - metallophosphoesterase, at 3,134,069 to 3,134,875
_RS13755
BPHYT_RS13760: BPHYT_RS13760 - hypothetical protein, at 3,135,010 to 3,135,732
_RS13760
Position (kb)
3132
3133
3134
3135 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3132.044 kb on + strand at 3132.081 kb on - strand, within BPHYT_RS13745 at 3132.267 kb on + strand, within BPHYT_RS13745 at 3132.267 kb on + strand, within BPHYT_RS13745 at 3132.269 kb on + strand, within BPHYT_RS13745 at 3132.269 kb on + strand, within BPHYT_RS13745 at 3132.270 kb on - strand, within BPHYT_RS13745 at 3132.270 kb on - strand, within BPHYT_RS13745 at 3132.685 kb on - strand at 3132.685 kb on - strand at 3133.180 kb on + strand, within BPHYT_RS13750 at 3133.180 kb on + strand, within BPHYT_RS13750 at 3133.181 kb on - strand, within BPHYT_RS13750 at 3133.767 kb on + strand, within BPHYT_RS13750 at 3133.768 kb on - strand, within BPHYT_RS13750 at 3133.818 kb on + strand, within BPHYT_RS13750 at 3133.818 kb on + strand, within BPHYT_RS13750 at 3133.846 kb on - strand, within BPHYT_RS13750 at 3133.926 kb on + strand at 3133.927 kb on - strand at 3133.927 kb on - strand at 3133.928 kb on + strand at 3133.928 kb on + strand at 3133.929 kb on - strand at 3133.929 kb on - strand at 3133.929 kb on - strand at 3134.011 kb on + strand at 3134.012 kb on - strand at 3134.231 kb on + strand, within BPHYT_RS13755 at 3134.231 kb on + strand, within BPHYT_RS13755 at 3134.256 kb on - strand, within BPHYT_RS13755 at 3134.405 kb on + strand, within BPHYT_RS13755 at 3134.753 kb on - strand, within BPHYT_RS13755 at 3134.753 kb on - strand, within BPHYT_RS13755 at 3134.857 kb on + strand at 3134.871 kb on + strand at 3134.871 kb on + strand at 3134.873 kb on + strand at 3134.874 kb on - strand at 3134.874 kb on - strand at 3134.874 kb on - strand at 3134.932 kb on + strand at 3134.932 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 3,132,044 + +1.9 3,132,081 - BPHYT_RS13745 0.13 -0.3 3,132,267 + BPHYT_RS13745 0.48 +0.9 3,132,267 + BPHYT_RS13745 0.48 +0.3 3,132,269 + BPHYT_RS13745 0.48 +0.3 3,132,269 + BPHYT_RS13745 0.48 -1.7 3,132,270 - BPHYT_RS13745 0.49 -0.3 3,132,270 - BPHYT_RS13745 0.49 -1.3 3,132,685 - -0.4 3,132,685 - -0.4 3,133,180 + BPHYT_RS13750 0.41 -1.1 3,133,180 + BPHYT_RS13750 0.41 -0.6 3,133,181 - BPHYT_RS13750 0.41 -1.0 3,133,767 + BPHYT_RS13750 0.82 -0.8 3,133,768 - BPHYT_RS13750 0.82 -0.9 3,133,818 + BPHYT_RS13750 0.86 -0.4 3,133,818 + BPHYT_RS13750 0.86 -0.5 3,133,846 - BPHYT_RS13750 0.88 -1.3 3,133,926 + -2.7 3,133,927 - -1.2 3,133,927 - -0.8 3,133,928 + -1.0 3,133,928 + -1.0 3,133,929 - -0.4 3,133,929 - -0.4 3,133,929 - -0.1 3,134,011 + -0.9 3,134,012 - -0.9 3,134,231 + BPHYT_RS13755 0.20 +0.7 3,134,231 + BPHYT_RS13755 0.20 -0.0 3,134,256 - BPHYT_RS13755 0.23 -0.5 3,134,405 + BPHYT_RS13755 0.42 -0.0 3,134,753 - BPHYT_RS13755 0.85 -0.9 3,134,753 - BPHYT_RS13755 0.85 -0.1 3,134,857 + +1.9 3,134,871 + +1.7 3,134,871 + +0.1 3,134,873 + +0.3 3,134,874 - +0.3 3,134,874 - +0.4 3,134,874 - +0.3 3,134,932 + +0.5 3,134,932 + -0.0
Or see this region's nucleotide sequence