Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10065 and BPHYT_RS10070 are separated by 44 nucleotides BPHYT_RS10070 and BPHYT_RS10075 are separated by 98 nucleotides BPHYT_RS10075 and BPHYT_RS10080 are separated by 62 nucleotides BPHYT_RS10080 and BPHYT_RS10085 are separated by 54 nucleotides
BPHYT_RS10065: BPHYT_RS10065 - cytochrome C oxidase subunit I, at 2,292,662 to 2,293,306
_RS10065
BPHYT_RS10070: BPHYT_RS10070 - hypothetical protein, at 2,293,351 to 2,293,734
_RS10070
BPHYT_RS10075: BPHYT_RS10075 - alcohol dehydrogenase, at 2,293,833 to 2,295,134
_RS10075
BPHYT_RS10080: BPHYT_RS10080 - hypothetical protein, at 2,295,197 to 2,295,415
_RS10080
BPHYT_RS10085: BPHYT_RS10085 - thiamine pyrophosphate protein, at 2,295,470 to 2,297,311
_RS10085
Position (kb)
2293
2294
2295
2296 Strain fitness (log2 ratio)
-2
-1
0
1 at 2293.005 kb on + strand, within BPHYT_RS10065 at 2293.080 kb on - strand, within BPHYT_RS10065 at 2293.102 kb on + strand, within BPHYT_RS10065 at 2293.514 kb on + strand, within BPHYT_RS10070 at 2293.515 kb on - strand, within BPHYT_RS10070 at 2293.749 kb on - strand at 2294.668 kb on - strand, within BPHYT_RS10075 at 2294.703 kb on + strand, within BPHYT_RS10075 at 2295.132 kb on + strand at 2295.156 kb on + strand at 2295.471 kb on + strand at 2295.472 kb on - strand at 2295.598 kb on + strand at 2295.598 kb on + strand at 2295.711 kb on + strand, within BPHYT_RS10085 at 2295.712 kb on - strand, within BPHYT_RS10085 at 2295.767 kb on - strand, within BPHYT_RS10085
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 2,293,005 + BPHYT_RS10065 0.53 -2.6 2,293,080 - BPHYT_RS10065 0.65 +0.6 2,293,102 + BPHYT_RS10065 0.68 -1.5 2,293,514 + BPHYT_RS10070 0.42 -1.3 2,293,515 - BPHYT_RS10070 0.43 +0.6 2,293,749 - -1.1 2,294,668 - BPHYT_RS10075 0.64 -0.8 2,294,703 + BPHYT_RS10075 0.67 +1.2 2,295,132 + -0.4 2,295,156 + +0.5 2,295,471 + +0.2 2,295,472 - -0.1 2,295,598 + -0.1 2,295,598 + +0.6 2,295,711 + BPHYT_RS10085 0.13 +0.4 2,295,712 - BPHYT_RS10085 0.13 -0.6 2,295,767 - BPHYT_RS10085 0.16 +0.5
Or see this region's nucleotide sequence