Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10075

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10065 and BPHYT_RS10070 are separated by 44 nucleotidesBPHYT_RS10070 and BPHYT_RS10075 are separated by 98 nucleotidesBPHYT_RS10075 and BPHYT_RS10080 are separated by 62 nucleotidesBPHYT_RS10080 and BPHYT_RS10085 are separated by 54 nucleotides BPHYT_RS10065: BPHYT_RS10065 - cytochrome C oxidase subunit I, at 2,292,662 to 2,293,306 _RS10065 BPHYT_RS10070: BPHYT_RS10070 - hypothetical protein, at 2,293,351 to 2,293,734 _RS10070 BPHYT_RS10075: BPHYT_RS10075 - alcohol dehydrogenase, at 2,293,833 to 2,295,134 _RS10075 BPHYT_RS10080: BPHYT_RS10080 - hypothetical protein, at 2,295,197 to 2,295,415 _RS10080 BPHYT_RS10085: BPHYT_RS10085 - thiamine pyrophosphate protein, at 2,295,470 to 2,297,311 _RS10085 Position (kb) 2293 2294 2295 2296Strain fitness (log2 ratio) -2 -1 0 1at 2293.005 kb on + strand, within BPHYT_RS10065at 2293.080 kb on - strand, within BPHYT_RS10065at 2293.102 kb on + strand, within BPHYT_RS10065at 2293.514 kb on + strand, within BPHYT_RS10070at 2293.515 kb on - strand, within BPHYT_RS10070at 2293.749 kb on - strandat 2294.668 kb on - strand, within BPHYT_RS10075at 2294.703 kb on + strand, within BPHYT_RS10075at 2295.132 kb on + strandat 2295.156 kb on + strandat 2295.471 kb on + strandat 2295.472 kb on - strandat 2295.598 kb on + strandat 2295.598 kb on + strandat 2295.711 kb on + strand, within BPHYT_RS10085at 2295.712 kb on - strand, within BPHYT_RS10085at 2295.767 kb on - strand, within BPHYT_RS10085

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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2,293,005 + BPHYT_RS10065 0.53 -2.6
2,293,080 - BPHYT_RS10065 0.65 +0.6
2,293,102 + BPHYT_RS10065 0.68 -1.5
2,293,514 + BPHYT_RS10070 0.42 -1.3
2,293,515 - BPHYT_RS10070 0.43 +0.6
2,293,749 - -1.1
2,294,668 - BPHYT_RS10075 0.64 -0.8
2,294,703 + BPHYT_RS10075 0.67 +1.2
2,295,132 + -0.4
2,295,156 + +0.5
2,295,471 + +0.2
2,295,472 - -0.1
2,295,598 + -0.1
2,295,598 + +0.6
2,295,711 + BPHYT_RS10085 0.13 +0.4
2,295,712 - BPHYT_RS10085 0.13 -0.6
2,295,767 - BPHYT_RS10085 0.16 +0.5

Or see this region's nucleotide sequence