Experiment: 1664 hydathodes 6 dpi (Dna extract from 8 * 200 hydathodes and pooled together)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Xcc-8004.4429.1 and Xcc-8004.4430.1 are separated by 10 nucleotides Xcc-8004.4430.1 and Xcc-8004.4431.1 are separated by 264 nucleotides Xcc-8004.4431.1 and Xcc-8004.4432.1 are separated by 214 nucleotides
Xcc-8004.4429.1: Xcc-8004.4429.1 - General secretion pathway protein H, at 4,237,049 to 4,237,558
Xcc-8004.4429.1
Xcc-8004.4430.1: Xcc-8004.4430.1 - General secretion pathway protein G, at 4,237,569 to 4,238,000
Xcc-8004.4430.1
Xcc-8004.4431.1: Xcc-8004.4431.1 - General secretion pathway protein F, at 4,238,265 to 4,239,482
Xcc-8004.4431.1
Xcc-8004.4432.1: Xcc-8004.4432.1 - General secretion pathway protein E, at 4,239,697 to 4,241,424
Xcc-8004.4432.1
Position (kb)
4238
4239
4240 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4237.351 kb on - strand, within Xcc-8004.4429.1 at 4237.571 kb on - strand at 4237.571 kb on - strand at 4237.576 kb on + strand at 4237.577 kb on - strand at 4237.594 kb on + strand at 4237.594 kb on + strand at 4237.595 kb on - strand at 4237.669 kb on + strand, within Xcc-8004.4430.1 at 4237.784 kb on + strand, within Xcc-8004.4430.1 at 4238.231 kb on + strand at 4238.232 kb on - strand at 4238.232 kb on - strand at 4238.232 kb on - strand at 4238.290 kb on + strand at 4238.290 kb on + strand at 4238.290 kb on + strand at 4238.290 kb on + strand at 4238.290 kb on + strand at 4238.291 kb on - strand at 4238.291 kb on - strand at 4238.772 kb on + strand, within Xcc-8004.4431.1 at 4238.772 kb on + strand, within Xcc-8004.4431.1 at 4238.773 kb on - strand, within Xcc-8004.4431.1 at 4238.773 kb on - strand, within Xcc-8004.4431.1 at 4238.885 kb on - strand, within Xcc-8004.4431.1 at 4238.885 kb on - strand, within Xcc-8004.4431.1 at 4238.885 kb on - strand, within Xcc-8004.4431.1 at 4238.885 kb on - strand, within Xcc-8004.4431.1 at 4238.885 kb on - strand, within Xcc-8004.4431.1 at 4238.911 kb on + strand, within Xcc-8004.4431.1 at 4238.912 kb on - strand, within Xcc-8004.4431.1 at 4238.912 kb on - strand, within Xcc-8004.4431.1 at 4238.966 kb on - strand, within Xcc-8004.4431.1 at 4239.085 kb on + strand, within Xcc-8004.4431.1 at 4239.085 kb on + strand, within Xcc-8004.4431.1 at 4239.086 kb on - strand, within Xcc-8004.4431.1 at 4239.086 kb on - strand, within Xcc-8004.4431.1 at 4239.483 kb on + strand at 4239.483 kb on + strand at 4239.484 kb on - strand at 4239.638 kb on + strand at 4239.638 kb on + strand at 4239.655 kb on + strand at 4239.655 kb on + strand at 4239.655 kb on + strand at 4239.656 kb on - strand at 4239.656 kb on - strand at 4239.773 kb on + strand at 4239.773 kb on + strand at 4239.773 kb on + strand at 4239.773 kb on + strand at 4239.773 kb on + strand at 4239.774 kb on - strand at 4239.774 kb on - strand at 4239.774 kb on - strand at 4239.774 kb on - strand at 4239.886 kb on - strand, within Xcc-8004.4432.1 at 4239.936 kb on - strand, within Xcc-8004.4432.1 at 4239.936 kb on - strand, within Xcc-8004.4432.1 at 4240.008 kb on - strand, within Xcc-8004.4432.1 at 4240.008 kb on - strand, within Xcc-8004.4432.1 at 4240.085 kb on + strand, within Xcc-8004.4432.1 at 4240.086 kb on - strand, within Xcc-8004.4432.1 at 4240.086 kb on - strand, within Xcc-8004.4432.1 at 4240.329 kb on - strand, within Xcc-8004.4432.1 at 4240.329 kb on - strand, within Xcc-8004.4432.1 at 4240.394 kb on + strand, within Xcc-8004.4432.1 at 4240.395 kb on - strand, within Xcc-8004.4432.1 at 4240.395 kb on - strand, within Xcc-8004.4432.1
Per-strain Table
Position Strand Gene LocusTag Fraction 1664 hydathodes 6 dpi (Dna extract from 8 * 200 hydathodes and pooled together) remove 4,237,351 - Xcc-8004.4429.1 0.59 -1.7 4,237,571 - +0.3 4,237,571 - -1.2 4,237,576 + -0.7 4,237,577 - +1.4 4,237,594 + -1.4 4,237,594 + +0.4 4,237,595 - -1.9 4,237,669 + Xcc-8004.4430.1 0.23 -2.0 4,237,784 + Xcc-8004.4430.1 0.50 -1.1 4,238,231 + +1.3 4,238,232 - -0.4 4,238,232 - +0.9 4,238,232 - +0.5 4,238,290 + -0.6 4,238,290 + -1.6 4,238,290 + -0.9 4,238,290 + -0.7 4,238,290 + -0.1 4,238,291 - -3.9 4,238,291 - -2.3 4,238,772 + Xcc-8004.4431.1 0.42 -0.1 4,238,772 + Xcc-8004.4431.1 0.42 -2.8 4,238,773 - Xcc-8004.4431.1 0.42 -3.1 4,238,773 - Xcc-8004.4431.1 0.42 -2.3 4,238,885 - Xcc-8004.4431.1 0.51 -1.1 4,238,885 - Xcc-8004.4431.1 0.51 -2.7 4,238,885 - Xcc-8004.4431.1 0.51 -2.1 4,238,885 - Xcc-8004.4431.1 0.51 -1.0 4,238,885 - Xcc-8004.4431.1 0.51 -2.3 4,238,911 + Xcc-8004.4431.1 0.53 -3.4 4,238,912 - Xcc-8004.4431.1 0.53 -1.7 4,238,912 - Xcc-8004.4431.1 0.53 +0.7 4,238,966 - Xcc-8004.4431.1 0.58 -0.7 4,239,085 + Xcc-8004.4431.1 0.67 -1.2 4,239,085 + Xcc-8004.4431.1 0.67 -1.0 4,239,086 - Xcc-8004.4431.1 0.67 +0.4 4,239,086 - Xcc-8004.4431.1 0.67 -0.8 4,239,483 + -2.0 4,239,483 + -0.8 4,239,484 - -0.7 4,239,638 + +1.6 4,239,638 + +1.2 4,239,655 + -0.5 4,239,655 + -0.1 4,239,655 + -0.7 4,239,656 - +0.1 4,239,656 - +3.4 4,239,773 + -1.2 4,239,773 + -0.4 4,239,773 + -2.3 4,239,773 + +0.1 4,239,773 + -1.2 4,239,774 - +0.1 4,239,774 - -0.3 4,239,774 - -1.4 4,239,774 - -1.0 4,239,886 - Xcc-8004.4432.1 0.11 -2.3 4,239,936 - Xcc-8004.4432.1 0.14 -0.7 4,239,936 - Xcc-8004.4432.1 0.14 -3.0 4,240,008 - Xcc-8004.4432.1 0.18 -1.5 4,240,008 - Xcc-8004.4432.1 0.18 -4.7 4,240,085 + Xcc-8004.4432.1 0.22 -1.0 4,240,086 - Xcc-8004.4432.1 0.23 -1.1 4,240,086 - Xcc-8004.4432.1 0.23 +0.9 4,240,329 - Xcc-8004.4432.1 0.37 -0.7 4,240,329 - Xcc-8004.4432.1 0.37 +0.3 4,240,394 + Xcc-8004.4432.1 0.40 -0.6 4,240,395 - Xcc-8004.4432.1 0.40 -1.6 4,240,395 - Xcc-8004.4432.1 0.40 -2.1
Or see this region's nucleotide sequence