Experiment: 1664 hydathodes 6 dpi (Dna extract from 8 * 200 hydathodes and pooled together)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Xcc-8004.4305.1 and Xcc-8004.4306.1 are separated by 265 nucleotides Xcc-8004.4306.1 and Xcc-8004.4307.1 are separated by 499 nucleotides Xcc-8004.4307.1 and Xcc-8004.4308.1 overlap by 11 nucleotides
Xcc-8004.4305.1: Xcc-8004.4305.1 - S-adenosylmethionine synthetase (EC 2.5.1.6), at 4,122,314 to 4,123,525
Xcc-8004.4305.1
Xcc-8004.4306.1: Xcc-8004.4306.1 - hypothetical protein, at 4,123,791 to 4,123,988
Xcc-8004.4306.1
Xcc-8004.4307.1: Xcc-8004.4307.1 - Probable histidine acid phosphatase (EC 3.1.3.2), at 4,124,488 to 4,125,819
Xcc-8004.4307.1
Xcc-8004.4308.1: Xcc-8004.4308.1 - hypothetical protein, at 4,125,809 to 4,128,610
Xcc-8004.4308.1
Position (kb)
4124
4125
4126 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4123.523 kb on + strand at 4123.623 kb on - strand at 4123.663 kb on - strand at 4123.678 kb on - strand at 4123.761 kb on + strand at 4123.761 kb on + strand at 4123.761 kb on + strand at 4123.762 kb on - strand at 4123.771 kb on + strand at 4123.772 kb on - strand at 4123.772 kb on - strand at 4123.799 kb on + strand at 4123.892 kb on - strand, within Xcc-8004.4306.1 at 4123.892 kb on - strand, within Xcc-8004.4306.1 at 4123.921 kb on + strand, within Xcc-8004.4306.1 at 4123.969 kb on + strand at 4124.032 kb on - strand at 4124.032 kb on - strand at 4124.139 kb on - strand at 4124.241 kb on + strand at 4124.241 kb on + strand at 4124.303 kb on + strand at 4124.304 kb on - strand at 4124.304 kb on - strand at 4124.304 kb on - strand at 4124.304 kb on - strand at 4124.321 kb on + strand at 4124.322 kb on - strand at 4124.375 kb on + strand at 4124.376 kb on - strand at 4124.399 kb on - strand at 4124.794 kb on - strand, within Xcc-8004.4307.1 at 4124.897 kb on + strand, within Xcc-8004.4307.1 at 4124.897 kb on + strand, within Xcc-8004.4307.1 at 4124.898 kb on - strand, within Xcc-8004.4307.1 at 4124.912 kb on + strand, within Xcc-8004.4307.1 at 4124.912 kb on + strand, within Xcc-8004.4307.1 at 4124.912 kb on + strand, within Xcc-8004.4307.1 at 4124.912 kb on + strand, within Xcc-8004.4307.1 at 4124.913 kb on - strand, within Xcc-8004.4307.1 at 4124.913 kb on - strand, within Xcc-8004.4307.1 at 4124.913 kb on - strand, within Xcc-8004.4307.1 at 4124.913 kb on - strand, within Xcc-8004.4307.1 at 4124.913 kb on - strand, within Xcc-8004.4307.1 at 4124.913 kb on - strand, within Xcc-8004.4307.1 at 4124.913 kb on - strand, within Xcc-8004.4307.1 at 4124.913 kb on - strand, within Xcc-8004.4307.1 at 4125.025 kb on + strand, within Xcc-8004.4307.1 at 4125.025 kb on + strand, within Xcc-8004.4307.1 at 4125.026 kb on - strand, within Xcc-8004.4307.1 at 4125.026 kb on - strand, within Xcc-8004.4307.1 at 4125.026 kb on - strand, within Xcc-8004.4307.1 at 4125.468 kb on - strand, within Xcc-8004.4307.1 at 4125.554 kb on + strand, within Xcc-8004.4307.1 at 4125.592 kb on + strand, within Xcc-8004.4307.1 at 4125.592 kb on + strand, within Xcc-8004.4307.1 at 4125.669 kb on + strand, within Xcc-8004.4307.1 at 4125.669 kb on + strand, within Xcc-8004.4307.1 at 4125.819 kb on + strand at 4125.819 kb on + strand at 4125.819 kb on + strand at 4125.819 kb on + strand at 4125.819 kb on + strand at 4125.820 kb on - strand at 4125.820 kb on - strand at 4125.900 kb on + strand at 4125.900 kb on + strand at 4125.901 kb on - strand at 4126.036 kb on - strand at 4126.065 kb on + strand at 4126.065 kb on + strand at 4126.065 kb on + strand at 4126.375 kb on - strand, within Xcc-8004.4308.1 at 4126.380 kb on + strand, within Xcc-8004.4308.1 at 4126.380 kb on + strand, within Xcc-8004.4308.1 at 4126.380 kb on + strand, within Xcc-8004.4308.1 at 4126.381 kb on - strand, within Xcc-8004.4308.1 at 4126.398 kb on + strand, within Xcc-8004.4308.1 at 4126.399 kb on - strand, within Xcc-8004.4308.1 at 4126.528 kb on + strand, within Xcc-8004.4308.1 at 4126.529 kb on - strand, within Xcc-8004.4308.1 at 4126.557 kb on - strand, within Xcc-8004.4308.1 at 4126.576 kb on - strand, within Xcc-8004.4308.1 at 4126.627 kb on - strand, within Xcc-8004.4308.1 at 4126.809 kb on + strand, within Xcc-8004.4308.1 at 4126.810 kb on - strand, within Xcc-8004.4308.1 at 4126.810 kb on - strand, within Xcc-8004.4308.1 at 4126.810 kb on - strand, within Xcc-8004.4308.1 at 4126.810 kb on - strand, within Xcc-8004.4308.1
Per-strain Table
Position Strand Gene LocusTag Fraction 1664 hydathodes 6 dpi (Dna extract from 8 * 200 hydathodes and pooled together) remove 4,123,523 + -0.7 4,123,623 - +1.5 4,123,663 - -0.3 4,123,678 - -0.5 4,123,761 + +3.0 4,123,761 + +1.7 4,123,761 + +0.4 4,123,762 - +2.9 4,123,771 + -1.4 4,123,772 - -0.5 4,123,772 - -1.4 4,123,799 + -0.2 4,123,892 - Xcc-8004.4306.1 0.51 +0.1 4,123,892 - Xcc-8004.4306.1 0.51 -1.4 4,123,921 + Xcc-8004.4306.1 0.66 +0.2 4,123,969 + +0.5 4,124,032 - -0.4 4,124,032 - +0.9 4,124,139 - -0.0 4,124,241 + +1.0 4,124,241 + -1.1 4,124,303 + +0.8 4,124,304 - -0.4 4,124,304 - -0.2 4,124,304 - +0.8 4,124,304 - -0.7 4,124,321 + +0.4 4,124,322 - -0.7 4,124,375 + -0.5 4,124,376 - +0.3 4,124,399 - -0.3 4,124,794 - Xcc-8004.4307.1 0.23 -0.3 4,124,897 + Xcc-8004.4307.1 0.31 -1.1 4,124,897 + Xcc-8004.4307.1 0.31 +3.3 4,124,898 - Xcc-8004.4307.1 0.31 +0.0 4,124,912 + Xcc-8004.4307.1 0.32 -1.2 4,124,912 + Xcc-8004.4307.1 0.32 +1.1 4,124,912 + Xcc-8004.4307.1 0.32 -1.8 4,124,912 + Xcc-8004.4307.1 0.32 -0.2 4,124,913 - Xcc-8004.4307.1 0.32 +0.9 4,124,913 - Xcc-8004.4307.1 0.32 -0.6 4,124,913 - Xcc-8004.4307.1 0.32 +1.8 4,124,913 - Xcc-8004.4307.1 0.32 +0.5 4,124,913 - Xcc-8004.4307.1 0.32 +0.3 4,124,913 - Xcc-8004.4307.1 0.32 +2.9 4,124,913 - Xcc-8004.4307.1 0.32 +0.8 4,124,913 - Xcc-8004.4307.1 0.32 +0.7 4,125,025 + Xcc-8004.4307.1 0.40 -0.3 4,125,025 + Xcc-8004.4307.1 0.40 -1.2 4,125,026 - Xcc-8004.4307.1 0.40 -1.1 4,125,026 - Xcc-8004.4307.1 0.40 -1.1 4,125,026 - Xcc-8004.4307.1 0.40 +1.0 4,125,468 - Xcc-8004.4307.1 0.74 +1.8 4,125,554 + Xcc-8004.4307.1 0.80 +1.1 4,125,592 + Xcc-8004.4307.1 0.83 +1.0 4,125,592 + Xcc-8004.4307.1 0.83 -0.4 4,125,669 + Xcc-8004.4307.1 0.89 -0.6 4,125,669 + Xcc-8004.4307.1 0.89 +2.0 4,125,819 + +1.0 4,125,819 + -3.1 4,125,819 + +0.6 4,125,819 + +1.0 4,125,819 + +0.7 4,125,820 - +0.5 4,125,820 - +3.1 4,125,900 + -2.1 4,125,900 + -0.1 4,125,901 - -1.8 4,126,036 - -0.2 4,126,065 + -0.9 4,126,065 + -1.1 4,126,065 + +0.3 4,126,375 - Xcc-8004.4308.1 0.20 +1.0 4,126,380 + Xcc-8004.4308.1 0.20 -0.2 4,126,380 + Xcc-8004.4308.1 0.20 -1.9 4,126,380 + Xcc-8004.4308.1 0.20 +1.2 4,126,381 - Xcc-8004.4308.1 0.20 -0.5 4,126,398 + Xcc-8004.4308.1 0.21 -2.6 4,126,399 - Xcc-8004.4308.1 0.21 -2.1 4,126,528 + Xcc-8004.4308.1 0.26 -0.4 4,126,529 - Xcc-8004.4308.1 0.26 -0.1 4,126,557 - Xcc-8004.4308.1 0.27 +0.7 4,126,576 - Xcc-8004.4308.1 0.27 +3.5 4,126,627 - Xcc-8004.4308.1 0.29 -3.3 4,126,809 + Xcc-8004.4308.1 0.36 +0.0 4,126,810 - Xcc-8004.4308.1 0.36 +0.3 4,126,810 - Xcc-8004.4308.1 0.36 +0.5 4,126,810 - Xcc-8004.4308.1 0.36 +0.9 4,126,810 - Xcc-8004.4308.1 0.36 +0.2
Or see this region's nucleotide sequence