Experiment: 1664 hydathodes 6 dpi (Dna extract from 8 * 200 hydathodes and pooled together)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Xcc-8004.3361.1 and Xcc-8004.3362.1 overlap by 1 nucleotides Xcc-8004.3362.1 and Xcc-8004.3363.1 overlap by 8 nucleotides Xcc-8004.3363.1 and Xcc-8004.3364.1 are separated by 120 nucleotides
Xcc-8004.3361.1: Xcc-8004.3361.1 - Phosphate transport system permease protein PstC (TC 3.A.1.7.1), at 3,254,941 to 3,255,915
Xcc-8004.3361.1
Xcc-8004.3362.1: Xcc-8004.3362.1 - Phosphate transport system permease protein PstA (TC 3.A.1.7.1), at 3,255,915 to 3,256,778
Xcc-8004.3362.1
Xcc-8004.3363.1: Xcc-8004.3363.1 - Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1), at 3,256,771 to 3,257,631
Xcc-8004.3363.1
Xcc-8004.3364.1: Xcc-8004.3364.1 - Phosphate transport system regulatory protein PhoU, at 3,257,752 to 3,258,462
Xcc-8004.3364.1
Position (kb) 3255
3256
3257 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 3255.169 kb on + strand, within Xcc-8004.3361.1 at 3255.169 kb on + strand, within Xcc-8004.3361.1 at 3255.170 kb on - strand, within Xcc-8004.3361.1 at 3255.190 kb on + strand, within Xcc-8004.3361.1 at 3255.343 kb on + strand, within Xcc-8004.3361.1 at 3255.343 kb on + strand, within Xcc-8004.3361.1 at 3255.344 kb on - strand, within Xcc-8004.3361.1 at 3255.565 kb on - strand, within Xcc-8004.3361.1 at 3255.628 kb on + strand, within Xcc-8004.3361.1 at 3255.657 kb on - strand, within Xcc-8004.3361.1 at 3255.805 kb on + strand, within Xcc-8004.3361.1 at 3255.939 kb on + strand at 3255.940 kb on - strand at 3255.940 kb on - strand at 3256.210 kb on - strand, within Xcc-8004.3362.1 at 3256.210 kb on - strand, within Xcc-8004.3362.1 at 3256.299 kb on + strand, within Xcc-8004.3362.1 at 3256.300 kb on - strand, within Xcc-8004.3362.1 at 3256.300 kb on - strand, within Xcc-8004.3362.1 at 3256.308 kb on + strand, within Xcc-8004.3362.1 at 3256.309 kb on - strand, within Xcc-8004.3362.1 at 3256.489 kb on - strand, within Xcc-8004.3362.1 at 3256.590 kb on + strand, within Xcc-8004.3362.1 at 3256.634 kb on - strand, within Xcc-8004.3362.1 at 3256.925 kb on - strand, within Xcc-8004.3363.1 at 3256.925 kb on - strand, within Xcc-8004.3363.1 at 3256.925 kb on - strand, within Xcc-8004.3363.1 at 3256.925 kb on - strand, within Xcc-8004.3363.1 at 3256.925 kb on - strand, within Xcc-8004.3363.1 at 3257.035 kb on + strand, within Xcc-8004.3363.1 at 3257.035 kb on + strand, within Xcc-8004.3363.1 at 3257.036 kb on - strand, within Xcc-8004.3363.1 at 3257.036 kb on - strand, within Xcc-8004.3363.1 at 3257.045 kb on - strand, within Xcc-8004.3363.1 at 3257.092 kb on - strand, within Xcc-8004.3363.1 at 3257.108 kb on - strand, within Xcc-8004.3363.1 at 3257.381 kb on + strand, within Xcc-8004.3363.1 at 3257.382 kb on - strand, within Xcc-8004.3363.1 at 3257.382 kb on - strand, within Xcc-8004.3363.1 at 3257.518 kb on + strand, within Xcc-8004.3363.1 at 3257.533 kb on + strand, within Xcc-8004.3363.1 at 3257.534 kb on - strand, within Xcc-8004.3363.1 at 3257.608 kb on + strand at 3257.609 kb on - strand at 3257.609 kb on - strand at 3257.609 kb on - strand at 3257.634 kb on + strand at 3257.634 kb on + strand at 3257.634 kb on + strand at 3257.634 kb on + strand at 3257.634 kb on + strand at 3257.635 kb on - strand at 3257.635 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1664 hydathodes 6 dpi (Dna extract from 8 * 200 hydathodes and pooled together) remove 3,255,169 + Xcc-8004.3361.1 0.23 -3.4 3,255,169 + Xcc-8004.3361.1 0.23 -3.8 3,255,170 - Xcc-8004.3361.1 0.23 -1.9 3,255,190 + Xcc-8004.3361.1 0.26 -2.0 3,255,343 + Xcc-8004.3361.1 0.41 -3.2 3,255,343 + Xcc-8004.3361.1 0.41 -1.4 3,255,344 - Xcc-8004.3361.1 0.41 -3.3 3,255,565 - Xcc-8004.3361.1 0.64 -2.1 3,255,628 + Xcc-8004.3361.1 0.70 -3.5 3,255,657 - Xcc-8004.3361.1 0.73 -1.4 3,255,805 + Xcc-8004.3361.1 0.89 -0.7 3,255,939 + -3.0 3,255,940 - -0.3 3,255,940 - -1.9 3,256,210 - Xcc-8004.3362.1 0.34 -1.1 3,256,210 - Xcc-8004.3362.1 0.34 -3.0 3,256,299 + Xcc-8004.3362.1 0.44 -3.3 3,256,300 - Xcc-8004.3362.1 0.45 -2.5 3,256,300 - Xcc-8004.3362.1 0.45 -2.9 3,256,308 + Xcc-8004.3362.1 0.45 -2.2 3,256,309 - Xcc-8004.3362.1 0.46 -1.6 3,256,489 - Xcc-8004.3362.1 0.66 -0.9 3,256,590 + Xcc-8004.3362.1 0.78 -2.3 3,256,634 - Xcc-8004.3362.1 0.83 -3.3 3,256,925 - Xcc-8004.3363.1 0.18 -3.0 3,256,925 - Xcc-8004.3363.1 0.18 -4.8 3,256,925 - Xcc-8004.3363.1 0.18 -0.3 3,256,925 - Xcc-8004.3363.1 0.18 -0.6 3,256,925 - Xcc-8004.3363.1 0.18 -1.6 3,257,035 + Xcc-8004.3363.1 0.31 -0.7 3,257,035 + Xcc-8004.3363.1 0.31 -2.6 3,257,036 - Xcc-8004.3363.1 0.31 -3.7 3,257,036 - Xcc-8004.3363.1 0.31 -2.1 3,257,045 - Xcc-8004.3363.1 0.32 -1.9 3,257,092 - Xcc-8004.3363.1 0.37 -2.5 3,257,108 - Xcc-8004.3363.1 0.39 -1.3 3,257,381 + Xcc-8004.3363.1 0.71 -1.3 3,257,382 - Xcc-8004.3363.1 0.71 -2.5 3,257,382 - Xcc-8004.3363.1 0.71 -1.2 3,257,518 + Xcc-8004.3363.1 0.87 +0.4 3,257,533 + Xcc-8004.3363.1 0.89 -2.5 3,257,534 - Xcc-8004.3363.1 0.89 -0.9 3,257,608 + -3.4 3,257,609 - -2.5 3,257,609 - +1.1 3,257,609 - -3.5 3,257,634 + +0.7 3,257,634 + -0.8 3,257,634 + -1.4 3,257,634 + +1.2 3,257,634 + -0.5 3,257,635 - -2.5 3,257,635 - -0.6
Or see this region's nucleotide sequence