Experiment: 1664 hydathodes 6 dpi (Dna extract from 8 * 200 hydathodes and pooled together)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Xcc-8004.1220.1 and Xcc-8004.1221.1 are separated by 112 nucleotides Xcc-8004.1221.1 and Xcc-8004.1222.1 overlap by 4 nucleotides Xcc-8004.1222.1 and Xcc-8004.1223.1 overlap by 4 nucleotides Xcc-8004.1223.1 and Xcc-8004.1224.1 are separated by 16 nucleotides
Xcc-8004.1220.1: Xcc-8004.1220.1 - Protein YicC, at 1,152,555 to 1,153,415
Xcc-8004.1220.1
Xcc-8004.1221.1: Xcc-8004.1221.1 - Ribonuclease PH (EC 2.7.7.56), at 1,153,528 to 1,154,253
Xcc-8004.1221.1
Xcc-8004.1222.1: Xcc-8004.1222.1 - Glyoxalase family protein, at 1,154,250 to 1,154,642
Xcc-8004.1222.1
Xcc-8004.1223.1: Xcc-8004.1223.1 - Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15), at 1,154,639 to 1,155,238
Xcc-8004.1223.1
Xcc-8004.1224.1: Xcc-8004.1224.1 - Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB, at 1,155,255 to 1,156,412
Xcc-8004.1224.1
Position (kb)
1154
1155 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1153.448 kb on - strand at 1153.453 kb on - strand at 1153.487 kb on + strand at 1153.723 kb on + strand, within Xcc-8004.1221.1 at 1153.936 kb on + strand, within Xcc-8004.1221.1 at 1153.936 kb on + strand, within Xcc-8004.1221.1 at 1153.936 kb on + strand, within Xcc-8004.1221.1 at 1153.936 kb on + strand, within Xcc-8004.1221.1 at 1153.937 kb on - strand, within Xcc-8004.1221.1 at 1153.937 kb on - strand, within Xcc-8004.1221.1 at 1153.937 kb on - strand, within Xcc-8004.1221.1 at 1153.937 kb on - strand, within Xcc-8004.1221.1 at 1154.031 kb on + strand, within Xcc-8004.1221.1 at 1154.031 kb on + strand, within Xcc-8004.1221.1 at 1154.032 kb on - strand, within Xcc-8004.1221.1 at 1154.036 kb on - strand, within Xcc-8004.1221.1 at 1154.313 kb on + strand, within Xcc-8004.1222.1 at 1154.313 kb on + strand, within Xcc-8004.1222.1 at 1154.314 kb on - strand, within Xcc-8004.1222.1 at 1154.494 kb on - strand, within Xcc-8004.1222.1 at 1154.623 kb on + strand at 1154.623 kb on + strand at 1154.623 kb on + strand at 1154.624 kb on - strand at 1154.889 kb on - strand, within Xcc-8004.1223.1 at 1154.993 kb on + strand, within Xcc-8004.1223.1 at 1155.105 kb on - strand, within Xcc-8004.1223.1 at 1155.288 kb on + strand at 1155.288 kb on + strand at 1155.288 kb on + strand at 1155.288 kb on + strand at 1155.288 kb on + strand at 1155.288 kb on + strand at 1155.289 kb on - strand at 1155.325 kb on - strand at 1155.375 kb on + strand, within Xcc-8004.1224.1 at 1155.376 kb on - strand, within Xcc-8004.1224.1 at 1155.376 kb on - strand, within Xcc-8004.1224.1 at 1155.598 kb on - strand, within Xcc-8004.1224.1
Per-strain Table
Position Strand Gene LocusTag Fraction 1664 hydathodes 6 dpi (Dna extract from 8 * 200 hydathodes and pooled together) remove 1,153,448 - +0.2 1,153,453 - -2.4 1,153,487 + +1.6 1,153,723 + Xcc-8004.1221.1 0.27 +1.0 1,153,936 + Xcc-8004.1221.1 0.56 +0.1 1,153,936 + Xcc-8004.1221.1 0.56 -2.4 1,153,936 + Xcc-8004.1221.1 0.56 +2.3 1,153,936 + Xcc-8004.1221.1 0.56 -1.2 1,153,937 - Xcc-8004.1221.1 0.56 +0.8 1,153,937 - Xcc-8004.1221.1 0.56 -0.8 1,153,937 - Xcc-8004.1221.1 0.56 -1.6 1,153,937 - Xcc-8004.1221.1 0.56 +0.2 1,154,031 + Xcc-8004.1221.1 0.69 +1.7 1,154,031 + Xcc-8004.1221.1 0.69 -1.6 1,154,032 - Xcc-8004.1221.1 0.69 -0.4 1,154,036 - Xcc-8004.1221.1 0.70 +4.7 1,154,313 + Xcc-8004.1222.1 0.16 +0.9 1,154,313 + Xcc-8004.1222.1 0.16 -1.9 1,154,314 - Xcc-8004.1222.1 0.16 -2.0 1,154,494 - Xcc-8004.1222.1 0.62 -2.7 1,154,623 + -0.6 1,154,623 + +0.4 1,154,623 + +1.8 1,154,624 - +0.0 1,154,889 - Xcc-8004.1223.1 0.42 -1.0 1,154,993 + Xcc-8004.1223.1 0.59 -0.9 1,155,105 - Xcc-8004.1223.1 0.78 +1.6 1,155,288 + -1.7 1,155,288 + -0.2 1,155,288 + -2.3 1,155,288 + -1.6 1,155,288 + +0.5 1,155,288 + -0.6 1,155,289 - +0.2 1,155,325 - +0.8 1,155,375 + Xcc-8004.1224.1 0.10 +0.6 1,155,376 - Xcc-8004.1224.1 0.10 -1.8 1,155,376 - Xcc-8004.1224.1 0.10 +0.8 1,155,598 - Xcc-8004.1224.1 0.30 +0.7
Or see this region's nucleotide sequence