Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS00945

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS00940 and MMJJ_RS00945 are separated by 139 nucleotidesMMJJ_RS00945 and MMJJ_RS00950 are separated by 104 nucleotidesMMJJ_RS00950 and MMJJ_RS00955 are separated by 30 nucleotides MMJJ_RS00940: MMJJ_RS00940 - aldo/keto reductase, at 181,899 to 183,092 _RS00940 MMJJ_RS00945: MMJJ_RS00945 - carboxymuconolactone decarboxylase family protein, at 183,232 to 183,963 _RS00945 MMJJ_RS00950: MMJJ_RS00950 - DUF111 family protein, at 184,068 to 184,787 _RS00950 MMJJ_RS00955: MMJJ_RS00955 - flavodoxin, at 184,818 to 185,353 _RS00955 Position (kb) 183 184Strain fitness (log2 ratio) -2 -1 0 1 2at 182.250 kb on - strand, within MMJJ_RS00940at 182.251 kb on + strand, within MMJJ_RS00940at 182.251 kb on + strand, within MMJJ_RS00940at 182.251 kb on + strand, within MMJJ_RS00940at 182.251 kb on + strand, within MMJJ_RS00940at 182.251 kb on + strand, within MMJJ_RS00940at 182.251 kb on + strand, within MMJJ_RS00940at 182.308 kb on + strand, within MMJJ_RS00940at 182.308 kb on + strand, within MMJJ_RS00940at 182.397 kb on - strand, within MMJJ_RS00940at 182.415 kb on + strand, within MMJJ_RS00940at 182.521 kb on - strand, within MMJJ_RS00940at 182.522 kb on + strand, within MMJJ_RS00940at 182.560 kb on + strand, within MMJJ_RS00940at 182.563 kb on - strand, within MMJJ_RS00940at 182.568 kb on + strand, within MMJJ_RS00940at 182.570 kb on + strand, within MMJJ_RS00940at 182.574 kb on - strand, within MMJJ_RS00940at 182.574 kb on - strand, within MMJJ_RS00940at 182.574 kb on - strand, within MMJJ_RS00940at 182.574 kb on - strand, within MMJJ_RS00940at 182.574 kb on - strand, within MMJJ_RS00940at 182.575 kb on + strand, within MMJJ_RS00940at 182.575 kb on + strand, within MMJJ_RS00940at 182.575 kb on + strand, within MMJJ_RS00940at 182.575 kb on + strand, within MMJJ_RS00940at 182.575 kb on + strand, within MMJJ_RS00940at 182.575 kb on + strand, within MMJJ_RS00940at 182.575 kb on + strand, within MMJJ_RS00940at 182.596 kb on - strand, within MMJJ_RS00940at 182.664 kb on + strand, within MMJJ_RS00940at 182.664 kb on + strand, within MMJJ_RS00940at 182.698 kb on + strand, within MMJJ_RS00940at 182.736 kb on + strand, within MMJJ_RS00940at 182.749 kb on + strand, within MMJJ_RS00940at 182.752 kb on - strand, within MMJJ_RS00940at 182.762 kb on - strand, within MMJJ_RS00940at 182.768 kb on - strand, within MMJJ_RS00940at 182.768 kb on - strand, within MMJJ_RS00940at 182.769 kb on + strand, within MMJJ_RS00940at 182.805 kb on + strand, within MMJJ_RS00940at 182.810 kb on - strand, within MMJJ_RS00940at 182.826 kb on - strand, within MMJJ_RS00940at 182.859 kb on + strand, within MMJJ_RS00940at 183.021 kb on + strandat 183.024 kb on - strandat 183.110 kb on - strandat 183.111 kb on + strandat 183.113 kb on + strandat 183.126 kb on - strandat 183.150 kb on + strandat 183.166 kb on - strandat 183.166 kb on - strandat 183.166 kb on - strandat 183.171 kb on - strandat 183.172 kb on + strandat 183.200 kb on - strandat 183.200 kb on - strandat 183.200 kb on - strandat 183.200 kb on - strandat 183.201 kb on + strandat 183.201 kb on + strandat 183.201 kb on + strandat 183.201 kb on + strandat 183.206 kb on - strandat 183.210 kb on + strandat 183.215 kb on + strandat 183.234 kb on + strandat 183.234 kb on + strandat 183.253 kb on - strandat 183.289 kb on - strandat 183.310 kb on + strand, within MMJJ_RS00945at 183.322 kb on - strand, within MMJJ_RS00945at 183.323 kb on + strand, within MMJJ_RS00945at 183.323 kb on + strand, within MMJJ_RS00945at 183.325 kb on - strand, within MMJJ_RS00945at 183.325 kb on - strand, within MMJJ_RS00945at 183.363 kb on - strand, within MMJJ_RS00945at 183.364 kb on + strand, within MMJJ_RS00945at 183.364 kb on + strand, within MMJJ_RS00945at 183.374 kb on + strand, within MMJJ_RS00945at 183.407 kb on + strand, within MMJJ_RS00945at 183.407 kb on + strand, within MMJJ_RS00945at 183.408 kb on - strand, within MMJJ_RS00945at 183.408 kb on - strand, within MMJJ_RS00945at 183.458 kb on - strand, within MMJJ_RS00945at 183.459 kb on + strand, within MMJJ_RS00945at 183.459 kb on + strand, within MMJJ_RS00945at 183.459 kb on + strand, within MMJJ_RS00945at 183.488 kb on - strand, within MMJJ_RS00945at 183.488 kb on - strand, within MMJJ_RS00945at 183.502 kb on - strand, within MMJJ_RS00945at 183.502 kb on - strand, within MMJJ_RS00945at 183.502 kb on - strand, within MMJJ_RS00945at 183.527 kb on - strand, within MMJJ_RS00945at 183.528 kb on + strand, within MMJJ_RS00945at 183.589 kb on + strand, within MMJJ_RS00945at 183.620 kb on + strand, within MMJJ_RS00945at 183.625 kb on - strand, within MMJJ_RS00945at 183.631 kb on - strand, within MMJJ_RS00945at 183.678 kb on + strand, within MMJJ_RS00945at 183.678 kb on + strand, within MMJJ_RS00945at 183.698 kb on - strand, within MMJJ_RS00945at 183.698 kb on - strand, within MMJJ_RS00945at 183.713 kb on - strand, within MMJJ_RS00945at 183.713 kb on - strand, within MMJJ_RS00945at 183.715 kb on - strand, within MMJJ_RS00945at 183.715 kb on - strand, within MMJJ_RS00945at 183.715 kb on - strand, within MMJJ_RS00945at 183.715 kb on - strand, within MMJJ_RS00945at 183.716 kb on + strand, within MMJJ_RS00945at 183.716 kb on + strand, within MMJJ_RS00945at 183.718 kb on - strand, within MMJJ_RS00945at 183.718 kb on - strand, within MMJJ_RS00945at 183.719 kb on + strand, within MMJJ_RS00945at 183.719 kb on + strand, within MMJJ_RS00945at 183.735 kb on - strand, within MMJJ_RS00945at 183.735 kb on - strand, within MMJJ_RS00945at 183.742 kb on - strand, within MMJJ_RS00945at 183.773 kb on + strand, within MMJJ_RS00945at 183.777 kb on - strand, within MMJJ_RS00945at 183.778 kb on + strand, within MMJJ_RS00945at 183.861 kb on - strand, within MMJJ_RS00945at 183.862 kb on + strand, within MMJJ_RS00945at 183.914 kb on + strandat 183.918 kb on - strandat 183.918 kb on - strandat 183.964 kb on + strandat 184.025 kb on - strandat 184.028 kb on - strandat 184.028 kb on - strandat 184.029 kb on + strandat 184.029 kb on + strandat 184.031 kb on + strandat 184.037 kb on - strandat 184.046 kb on + strandat 184.046 kb on + strandat 184.046 kb on + strandat 184.053 kb on + strandat 184.099 kb on + strandat 184.148 kb on - strand, within MMJJ_RS00950at 184.211 kb on - strand, within MMJJ_RS00950at 184.358 kb on + strand, within MMJJ_RS00950at 184.554 kb on - strand, within MMJJ_RS00950at 184.555 kb on + strand, within MMJJ_RS00950at 184.556 kb on - strand, within MMJJ_RS00950at 184.556 kb on - strand, within MMJJ_RS00950at 184.557 kb on + strand, within MMJJ_RS00950at 184.557 kb on + strand, within MMJJ_RS00950at 184.563 kb on - strand, within MMJJ_RS00950at 184.577 kb on + strand, within MMJJ_RS00950at 184.743 kb on + strandat 184.775 kb on - strandat 184.830 kb on - strandat 184.935 kb on + strand, within MMJJ_RS00955

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
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182,250 - MMJJ_RS00940 0.29 +0.8
182,251 + MMJJ_RS00940 0.29 +0.4
182,251 + MMJJ_RS00940 0.29 -0.2
182,251 + MMJJ_RS00940 0.29 -0.1
182,251 + MMJJ_RS00940 0.29 +0.8
182,251 + MMJJ_RS00940 0.29 -1.7
182,251 + MMJJ_RS00940 0.29 -0.0
182,308 + MMJJ_RS00940 0.34 +0.1
182,308 + MMJJ_RS00940 0.34 +0.7
182,397 - MMJJ_RS00940 0.42 +0.5
182,415 + MMJJ_RS00940 0.43 +0.4
182,521 - MMJJ_RS00940 0.52 +0.3
182,522 + MMJJ_RS00940 0.52 +0.2
182,560 + MMJJ_RS00940 0.55 +0.3
182,563 - MMJJ_RS00940 0.56 +0.4
182,568 + MMJJ_RS00940 0.56 +0.3
182,570 + MMJJ_RS00940 0.56 -0.1
182,574 - MMJJ_RS00940 0.57 +0.1
182,574 - MMJJ_RS00940 0.57 -1.6
182,574 - MMJJ_RS00940 0.57 +0.6
182,574 - MMJJ_RS00940 0.57 +2.4
182,574 - MMJJ_RS00940 0.57 -0.1
182,575 + MMJJ_RS00940 0.57 +0.5
182,575 + MMJJ_RS00940 0.57 +0.8
182,575 + MMJJ_RS00940 0.57 +0.7
182,575 + MMJJ_RS00940 0.57 -0.3
182,575 + MMJJ_RS00940 0.57 +1.3
182,575 + MMJJ_RS00940 0.57 +0.5
182,575 + MMJJ_RS00940 0.57 +0.3
182,596 - MMJJ_RS00940 0.58 -0.5
182,664 + MMJJ_RS00940 0.64 -0.1
182,664 + MMJJ_RS00940 0.64 -0.2
182,698 + MMJJ_RS00940 0.67 -0.5
182,736 + MMJJ_RS00940 0.70 -0.3
182,749 + MMJJ_RS00940 0.71 -0.3
182,752 - MMJJ_RS00940 0.71 -0.7
182,762 - MMJJ_RS00940 0.72 -0.7
182,768 - MMJJ_RS00940 0.73 -0.0
182,768 - MMJJ_RS00940 0.73 +0.5
182,769 + MMJJ_RS00940 0.73 +0.2
182,805 + MMJJ_RS00940 0.76 +0.2
182,810 - MMJJ_RS00940 0.76 +0.2
182,826 - MMJJ_RS00940 0.78 +0.0
182,859 + MMJJ_RS00940 0.80 -0.7
183,021 + -0.6
183,024 - +0.5
183,110 - +0.4
183,111 + -0.4
183,113 + +0.5
183,126 - +0.4
183,150 + +0.3
183,166 - -0.2
183,166 - -0.2
183,166 - +0.4
183,171 - +1.3
183,172 + -0.4
183,200 - -0.7
183,200 - +0.1
183,200 - +0.0
183,200 - -0.3
183,201 + -0.9
183,201 + +0.1
183,201 + -0.6
183,201 + -0.6
183,206 - +0.1
183,210 + -0.3
183,215 + -0.3
183,234 + +0.1
183,234 + -0.4
183,253 - +0.5
183,289 - -0.9
183,310 + MMJJ_RS00945 0.11 +0.3
183,322 - MMJJ_RS00945 0.12 +0.2
183,323 + MMJJ_RS00945 0.12 +0.7
183,323 + MMJJ_RS00945 0.12 +0.1
183,325 - MMJJ_RS00945 0.13 -0.0
183,325 - MMJJ_RS00945 0.13 +1.8
183,363 - MMJJ_RS00945 0.18 +0.0
183,364 + MMJJ_RS00945 0.18 +0.1
183,364 + MMJJ_RS00945 0.18 +0.7
183,374 + MMJJ_RS00945 0.19 -0.5
183,407 + MMJJ_RS00945 0.24 +0.4
183,407 + MMJJ_RS00945 0.24 +0.5
183,408 - MMJJ_RS00945 0.24 +0.2
183,408 - MMJJ_RS00945 0.24 -0.7
183,458 - MMJJ_RS00945 0.31 +0.3
183,459 + MMJJ_RS00945 0.31 +0.5
183,459 + MMJJ_RS00945 0.31 -0.1
183,459 + MMJJ_RS00945 0.31 +1.0
183,488 - MMJJ_RS00945 0.35 +0.1
183,488 - MMJJ_RS00945 0.35 +0.1
183,502 - MMJJ_RS00945 0.37 +0.6
183,502 - MMJJ_RS00945 0.37 -0.6
183,502 - MMJJ_RS00945 0.37 +1.0
183,527 - MMJJ_RS00945 0.40 +0.1
183,528 + MMJJ_RS00945 0.40 +0.3
183,589 + MMJJ_RS00945 0.49 +0.3
183,620 + MMJJ_RS00945 0.53 -0.3
183,625 - MMJJ_RS00945 0.54 +0.6
183,631 - MMJJ_RS00945 0.55 +0.1
183,678 + MMJJ_RS00945 0.61 +0.0
183,678 + MMJJ_RS00945 0.61 -0.1
183,698 - MMJJ_RS00945 0.64 +1.8
183,698 - MMJJ_RS00945 0.64 -1.1
183,713 - MMJJ_RS00945 0.66 +0.1
183,713 - MMJJ_RS00945 0.66 +0.6
183,715 - MMJJ_RS00945 0.66 +0.5
183,715 - MMJJ_RS00945 0.66 -0.4
183,715 - MMJJ_RS00945 0.66 +0.3
183,715 - MMJJ_RS00945 0.66 -0.5
183,716 + MMJJ_RS00945 0.66 -0.2
183,716 + MMJJ_RS00945 0.66 +0.1
183,718 - MMJJ_RS00945 0.66 -0.0
183,718 - MMJJ_RS00945 0.66 -0.7
183,719 + MMJJ_RS00945 0.67 -0.4
183,719 + MMJJ_RS00945 0.67 -0.0
183,735 - MMJJ_RS00945 0.69 +0.4
183,735 - MMJJ_RS00945 0.69 +0.1
183,742 - MMJJ_RS00945 0.70 +0.4
183,773 + MMJJ_RS00945 0.74 +0.4
183,777 - MMJJ_RS00945 0.74 -0.2
183,778 + MMJJ_RS00945 0.75 +0.1
183,861 - MMJJ_RS00945 0.86 +1.0
183,862 + MMJJ_RS00945 0.86 -0.0
183,914 + -0.3
183,918 - -0.3
183,918 - +0.6
183,964 + -0.3
184,025 - -0.2
184,028 - +0.4
184,028 - +0.7
184,029 + -0.5
184,029 + -0.9
184,031 + +1.2
184,037 - -0.2
184,046 + +0.3
184,046 + -0.1
184,046 + +0.5
184,053 + +0.1
184,099 + +0.1
184,148 - MMJJ_RS00950 0.11 -0.5
184,211 - MMJJ_RS00950 0.20 +0.4
184,358 + MMJJ_RS00950 0.40 +0.4
184,554 - MMJJ_RS00950 0.68 +0.3
184,555 + MMJJ_RS00950 0.68 -0.0
184,556 - MMJJ_RS00950 0.68 -0.2
184,556 - MMJJ_RS00950 0.68 +0.4
184,557 + MMJJ_RS00950 0.68 +0.3
184,557 + MMJJ_RS00950 0.68 +0.2
184,563 - MMJJ_RS00950 0.69 -0.0
184,577 + MMJJ_RS00950 0.71 -0.2
184,743 + +0.7
184,775 - -0.7
184,830 - -0.3
184,935 + MMJJ_RS00955 0.22 -2.0

Or see this region's nucleotide sequence