Experiment: R2A with Chromium(III) Chloride Hexahydrate 0.078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS12185 and LRK54_RS12190 are separated by 81 nucleotides LRK54_RS12190 and LRK54_RS12200 are separated by 757 nucleotides
LRK54_RS12185: LRK54_RS12185 - penicillin-binding protein activator, at 2,710,103 to 2,712,037
_RS12185
LRK54_RS12190: LRK54_RS12190 - 16S rRNA (cytidine(1402)-2'-O)-methyltransferase, at 2,712,119 to 2,712,943
_RS12190
LRK54_RS12200: LRK54_RS12200 - division/cell wall cluster transcriptional repressor MraZ, at 2,713,701 to 2,714,147
_RS12200
Position (kb)
2712
2713 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2711.148 kb on + strand, within LRK54_RS12185 at 2711.148 kb on + strand, within LRK54_RS12185 at 2711.149 kb on - strand, within LRK54_RS12185 at 2711.196 kb on + strand, within LRK54_RS12185 at 2711.196 kb on + strand, within LRK54_RS12185 at 2711.196 kb on + strand, within LRK54_RS12185 at 2711.196 kb on + strand, within LRK54_RS12185 at 2711.197 kb on - strand, within LRK54_RS12185 at 2711.197 kb on - strand, within LRK54_RS12185 at 2711.197 kb on - strand, within LRK54_RS12185 at 2711.197 kb on - strand, within LRK54_RS12185 at 2711.241 kb on + strand, within LRK54_RS12185 at 2711.241 kb on + strand, within LRK54_RS12185 at 2711.241 kb on + strand, within LRK54_RS12185 at 2711.241 kb on + strand, within LRK54_RS12185 at 2711.241 kb on + strand, within LRK54_RS12185 at 2711.241 kb on + strand, within LRK54_RS12185 at 2711.241 kb on + strand, within LRK54_RS12185 at 2711.241 kb on + strand, within LRK54_RS12185 at 2711.241 kb on + strand, within LRK54_RS12185 at 2711.242 kb on - strand, within LRK54_RS12185 at 2711.242 kb on - strand, within LRK54_RS12185 at 2711.242 kb on - strand, within LRK54_RS12185 at 2711.242 kb on - strand, within LRK54_RS12185 at 2711.242 kb on - strand, within LRK54_RS12185 at 2711.242 kb on - strand, within LRK54_RS12185 at 2711.242 kb on - strand, within LRK54_RS12185 at 2711.242 kb on - strand, within LRK54_RS12185 at 2711.289 kb on + strand, within LRK54_RS12185 at 2711.289 kb on + strand, within LRK54_RS12185 at 2711.290 kb on - strand, within LRK54_RS12185 at 2711.290 kb on - strand, within LRK54_RS12185 at 2711.290 kb on - strand, within LRK54_RS12185 at 2711.290 kb on - strand, within LRK54_RS12185 at 2711.290 kb on - strand, within LRK54_RS12185 at 2711.290 kb on - strand, within LRK54_RS12185 at 2711.290 kb on - strand, within LRK54_RS12185 at 2711.290 kb on - strand, within LRK54_RS12185 at 2711.515 kb on - strand, within LRK54_RS12185 at 2711.515 kb on - strand, within LRK54_RS12185 at 2711.823 kb on - strand, within LRK54_RS12185 at 2711.924 kb on + strand at 2711.925 kb on - strand at 2712.078 kb on + strand at 2712.078 kb on + strand at 2712.078 kb on + strand at 2712.099 kb on + strand at 2712.099 kb on + strand at 2712.100 kb on - strand at 2712.100 kb on - strand at 2712.132 kb on + strand at 2712.132 kb on + strand at 2712.132 kb on + strand at 2712.132 kb on + strand at 2712.132 kb on + strand at 2712.133 kb on - strand at 2712.133 kb on - strand at 2712.133 kb on - strand at 2712.133 kb on - strand at 2712.133 kb on - strand at 2712.133 kb on - strand at 2712.278 kb on - strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.605 kb on + strand, within LRK54_RS12190 at 2712.606 kb on - strand, within LRK54_RS12190 at 2712.606 kb on - strand, within LRK54_RS12190 at 2712.606 kb on - strand, within LRK54_RS12190 at 2712.606 kb on - strand, within LRK54_RS12190 at 2712.606 kb on - strand, within LRK54_RS12190 at 2712.606 kb on - strand, within LRK54_RS12190 at 2712.606 kb on - strand, within LRK54_RS12190 at 2712.606 kb on - strand, within LRK54_RS12190 at 2712.606 kb on - strand, within LRK54_RS12190 at 2712.606 kb on - strand, within LRK54_RS12190 at 2712.606 kb on - strand, within LRK54_RS12190 at 2712.606 kb on - strand, within LRK54_RS12190 at 2712.606 kb on - strand, within LRK54_RS12190 at 2712.606 kb on - strand, within LRK54_RS12190 at 2712.606 kb on - strand, within LRK54_RS12190 at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.930 kb on + strand at 2712.931 kb on - strand at 2712.931 kb on - strand at 2712.931 kb on - strand at 2712.931 kb on - strand at 2712.931 kb on - strand at 2712.931 kb on - strand at 2712.931 kb on - strand at 2712.931 kb on - strand at 2712.931 kb on - strand at 2712.931 kb on - strand at 2712.931 kb on - strand at 2712.931 kb on - strand at 2712.932 kb on + strand at 2712.932 kb on + strand at 2712.932 kb on + strand at 2712.932 kb on + strand at 2712.932 kb on + strand at 2712.932 kb on + strand at 2712.932 kb on + strand at 2712.932 kb on + strand at 2712.932 kb on + strand at 2712.932 kb on + strand at 2712.932 kb on + strand at 2712.932 kb on + strand at 2712.932 kb on + strand at 2712.932 kb on + strand at 2712.932 kb on + strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2712.933 kb on - strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.001 kb on + strand at 2713.002 kb on - strand at 2713.002 kb on - strand at 2713.002 kb on - strand at 2713.002 kb on - strand at 2713.002 kb on - strand at 2713.002 kb on - strand at 2713.002 kb on - strand at 2713.002 kb on - strand at 2713.002 kb on - strand at 2713.002 kb on - strand at 2713.002 kb on - strand at 2713.002 kb on - strand at 2713.002 kb on - strand at 2713.002 kb on - strand at 2713.002 kb on - strand at 2713.569 kb on + strand at 2713.569 kb on + strand at 2713.569 kb on + strand at 2713.569 kb on + strand at 2713.569 kb on + strand at 2713.580 kb on + strand at 2713.580 kb on + strand at 2713.580 kb on + strand at 2713.600 kb on + strand at 2713.600 kb on + strand at 2713.600 kb on + strand at 2713.757 kb on + strand, within LRK54_RS12200 at 2713.757 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.812 kb on + strand, within LRK54_RS12200 at 2713.851 kb on + strand, within LRK54_RS12200 at 2713.851 kb on + strand, within LRK54_RS12200 at 2713.851 kb on + strand, within LRK54_RS12200
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chromium(III) Chloride Hexahydrate 0.078125 mM remove 2,711,148 + LRK54_RS12185 0.54 -1.2 2,711,148 + LRK54_RS12185 0.54 -0.5 2,711,149 - LRK54_RS12185 0.54 -1.6 2,711,196 + LRK54_RS12185 0.56 -1.3 2,711,196 + LRK54_RS12185 0.56 -2.7 2,711,196 + LRK54_RS12185 0.56 -0.5 2,711,196 + LRK54_RS12185 0.56 +1.0 2,711,197 - LRK54_RS12185 0.57 -1.2 2,711,197 - LRK54_RS12185 0.57 -1.7 2,711,197 - LRK54_RS12185 0.57 +0.3 2,711,197 - LRK54_RS12185 0.57 -1.2 2,711,241 + LRK54_RS12185 0.59 -0.5 2,711,241 + LRK54_RS12185 0.59 +0.6 2,711,241 + LRK54_RS12185 0.59 -1.0 2,711,241 + LRK54_RS12185 0.59 -0.8 2,711,241 + LRK54_RS12185 0.59 -1.6 2,711,241 + LRK54_RS12185 0.59 +0.0 2,711,241 + LRK54_RS12185 0.59 -1.7 2,711,241 + LRK54_RS12185 0.59 -1.6 2,711,241 + LRK54_RS12185 0.59 -0.3 2,711,242 - LRK54_RS12185 0.59 -2.0 2,711,242 - LRK54_RS12185 0.59 -1.5 2,711,242 - LRK54_RS12185 0.59 -1.1 2,711,242 - LRK54_RS12185 0.59 -1.4 2,711,242 - LRK54_RS12185 0.59 -1.4 2,711,242 - LRK54_RS12185 0.59 -0.2 2,711,242 - LRK54_RS12185 0.59 -1.8 2,711,242 - LRK54_RS12185 0.59 +0.8 2,711,289 + LRK54_RS12185 0.61 -2.8 2,711,289 + LRK54_RS12185 0.61 -0.0 2,711,290 - LRK54_RS12185 0.61 +2.0 2,711,290 - LRK54_RS12185 0.61 -0.6 2,711,290 - LRK54_RS12185 0.61 -1.5 2,711,290 - LRK54_RS12185 0.61 -1.1 2,711,290 - LRK54_RS12185 0.61 -0.2 2,711,290 - LRK54_RS12185 0.61 +0.3 2,711,290 - LRK54_RS12185 0.61 -0.8 2,711,290 - LRK54_RS12185 0.61 -0.7 2,711,515 - LRK54_RS12185 0.73 -0.6 2,711,515 - LRK54_RS12185 0.73 -2.6 2,711,823 - LRK54_RS12185 0.89 -1.5 2,711,924 + -0.7 2,711,925 - +0.0 2,712,078 + -0.8 2,712,078 + -1.6 2,712,078 + +0.5 2,712,099 + -0.2 2,712,099 + +1.1 2,712,100 - +0.1 2,712,100 - -0.3 2,712,132 + -0.3 2,712,132 + -0.2 2,712,132 + +1.3 2,712,132 + +1.0 2,712,132 + +0.0 2,712,133 - -0.1 2,712,133 - +0.0 2,712,133 - -0.6 2,712,133 - +0.2 2,712,133 - -1.1 2,712,133 - +2.1 2,712,278 - LRK54_RS12190 0.19 -0.5 2,712,605 + LRK54_RS12190 0.59 -1.2 2,712,605 + LRK54_RS12190 0.59 -1.6 2,712,605 + LRK54_RS12190 0.59 +0.3 2,712,605 + LRK54_RS12190 0.59 -2.3 2,712,605 + LRK54_RS12190 0.59 -1.8 2,712,605 + LRK54_RS12190 0.59 -1.5 2,712,605 + LRK54_RS12190 0.59 -1.2 2,712,605 + LRK54_RS12190 0.59 -1.9 2,712,605 + LRK54_RS12190 0.59 -0.7 2,712,605 + LRK54_RS12190 0.59 -1.4 2,712,605 + LRK54_RS12190 0.59 +0.0 2,712,605 + LRK54_RS12190 0.59 +0.3 2,712,605 + LRK54_RS12190 0.59 -1.2 2,712,605 + LRK54_RS12190 0.59 +0.9 2,712,605 + LRK54_RS12190 0.59 -0.5 2,712,605 + LRK54_RS12190 0.59 -2.2 2,712,605 + LRK54_RS12190 0.59 -2.7 2,712,605 + LRK54_RS12190 0.59 -1.2 2,712,605 + LRK54_RS12190 0.59 -0.8 2,712,605 + LRK54_RS12190 0.59 -0.5 2,712,605 + LRK54_RS12190 0.59 +1.3 2,712,606 - LRK54_RS12190 0.59 +1.3 2,712,606 - LRK54_RS12190 0.59 -2.0 2,712,606 - LRK54_RS12190 0.59 -3.4 2,712,606 - LRK54_RS12190 0.59 -2.1 2,712,606 - LRK54_RS12190 0.59 -3.5 2,712,606 - LRK54_RS12190 0.59 -2.4 2,712,606 - LRK54_RS12190 0.59 -2.2 2,712,606 - LRK54_RS12190 0.59 +0.0 2,712,606 - LRK54_RS12190 0.59 +0.5 2,712,606 - LRK54_RS12190 0.59 +0.0 2,712,606 - LRK54_RS12190 0.59 -1.8 2,712,606 - LRK54_RS12190 0.59 -0.7 2,712,606 - LRK54_RS12190 0.59 -1.7 2,712,606 - LRK54_RS12190 0.59 -1.7 2,712,606 - LRK54_RS12190 0.59 -3.1 2,712,930 + -1.2 2,712,930 + -0.3 2,712,930 + +0.4 2,712,930 + -1.1 2,712,930 + -0.7 2,712,930 + +0.1 2,712,930 + +1.9 2,712,930 + +0.0 2,712,930 + +0.5 2,712,930 + +0.8 2,712,930 + -0.9 2,712,930 + -2.2 2,712,930 + +0.2 2,712,930 + -0.3 2,712,930 + -1.2 2,712,930 + -0.7 2,712,930 + +0.3 2,712,930 + +1.3 2,712,930 + +0.5 2,712,930 + -0.5 2,712,930 + +0.3 2,712,930 + +1.1 2,712,930 + -2.0 2,712,931 - -1.1 2,712,931 - -2.5 2,712,931 - -1.2 2,712,931 - -3.1 2,712,931 - -1.0 2,712,931 - -1.0 2,712,931 - -1.7 2,712,931 - +1.8 2,712,931 - -1.3 2,712,931 - -1.9 2,712,931 - -0.7 2,712,931 - -2.1 2,712,932 + -2.5 2,712,932 + +0.0 2,712,932 + -0.2 2,712,932 + -1.1 2,712,932 + -1.8 2,712,932 + -1.6 2,712,932 + -3.6 2,712,932 + -1.1 2,712,932 + -1.5 2,712,932 + -0.7 2,712,932 + -1.7 2,712,932 + -1.4 2,712,932 + -1.8 2,712,932 + -2.4 2,712,932 + +2.6 2,712,933 - -1.2 2,712,933 - -0.2 2,712,933 - -0.8 2,712,933 - -1.0 2,712,933 - -1.9 2,712,933 - -2.4 2,712,933 - +0.0 2,712,933 - -2.4 2,712,933 - -1.7 2,712,933 - -0.5 2,712,933 - -2.4 2,712,933 - -2.9 2,712,933 - -2.6 2,712,933 - +0.8 2,712,933 - +0.0 2,712,933 - +1.3 2,712,933 - -1.2 2,712,933 - +0.3 2,712,933 - -0.6 2,712,933 - -0.8 2,712,933 - -0.7 2,712,933 - -2.4 2,712,933 - -1.2 2,712,933 - -1.1 2,712,933 - +0.3 2,712,933 - -1.5 2,712,933 - -1.6 2,712,933 - -1.4 2,712,933 - -1.3 2,713,001 + -0.6 2,713,001 + -2.0 2,713,001 + -0.8 2,713,001 + -0.6 2,713,001 + -1.4 2,713,001 + -1.0 2,713,001 + +0.3 2,713,001 + +0.4 2,713,001 + -2.1 2,713,001 + -2.4 2,713,001 + -0.6 2,713,001 + +2.1 2,713,001 + +1.1 2,713,001 + +0.9 2,713,001 + -1.0 2,713,001 + -1.6 2,713,001 + -0.4 2,713,001 + -0.2 2,713,001 + +1.1 2,713,001 + -0.4 2,713,001 + -1.3 2,713,001 + -0.2 2,713,001 + +0.4 2,713,001 + -0.5 2,713,002 - +0.3 2,713,002 - -0.4 2,713,002 - -0.4 2,713,002 - +1.0 2,713,002 - +0.2 2,713,002 - +0.2 2,713,002 - +1.5 2,713,002 - -0.7 2,713,002 - -1.1 2,713,002 - -0.0 2,713,002 - -1.5 2,713,002 - -0.0 2,713,002 - -0.4 2,713,002 - -1.6 2,713,002 - -2.4 2,713,569 + -0.7 2,713,569 + +0.0 2,713,569 + +0.3 2,713,569 + -1.7 2,713,569 + -1.6 2,713,580 + -0.2 2,713,580 + -0.3 2,713,580 + -2.5 2,713,600 + -0.8 2,713,600 + +0.0 2,713,600 + +0.0 2,713,757 + LRK54_RS12200 0.13 -0.1 2,713,757 + LRK54_RS12200 0.13 -0.0 2,713,812 + LRK54_RS12200 0.25 -1.5 2,713,812 + LRK54_RS12200 0.25 +1.6 2,713,812 + LRK54_RS12200 0.25 -0.9 2,713,812 + LRK54_RS12200 0.25 -2.0 2,713,812 + LRK54_RS12200 0.25 +1.3 2,713,812 + LRK54_RS12200 0.25 -0.2 2,713,812 + LRK54_RS12200 0.25 -1.0 2,713,812 + LRK54_RS12200 0.25 -1.3 2,713,812 + LRK54_RS12200 0.25 -0.9 2,713,812 + LRK54_RS12200 0.25 +0.5 2,713,812 + LRK54_RS12200 0.25 +1.0 2,713,812 + LRK54_RS12200 0.25 -0.5 2,713,812 + LRK54_RS12200 0.25 -1.0 2,713,812 + LRK54_RS12200 0.25 +1.0 2,713,812 + LRK54_RS12200 0.25 -0.9 2,713,812 + LRK54_RS12200 0.25 +0.5 2,713,812 + LRK54_RS12200 0.25 -1.2 2,713,812 + LRK54_RS12200 0.25 -1.5 2,713,812 + LRK54_RS12200 0.25 -1.6 2,713,812 + LRK54_RS12200 0.25 -1.2 2,713,812 + LRK54_RS12200 0.25 -2.4 2,713,812 + LRK54_RS12200 0.25 +0.1 2,713,812 + LRK54_RS12200 0.25 -1.8 2,713,812 + LRK54_RS12200 0.25 -1.2 2,713,812 + LRK54_RS12200 0.25 -0.8 2,713,812 + LRK54_RS12200 0.25 +0.3 2,713,812 + LRK54_RS12200 0.25 +0.2 2,713,812 + LRK54_RS12200 0.25 -1.0 2,713,812 + LRK54_RS12200 0.25 +0.8 2,713,812 + LRK54_RS12200 0.25 -1.3 2,713,812 + LRK54_RS12200 0.25 -2.5 2,713,851 + LRK54_RS12200 0.34 -2.6 2,713,851 + LRK54_RS12200 0.34 -1.3 2,713,851 + LRK54_RS12200 0.34 +0.3
Or see this region's nucleotide sequence