Experiment: R2A with Chromium(III) Chloride Hexahydrate 0.078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS04300 and LRK54_RS04305 are separated by 53 nucleotides LRK54_RS04305 and LRK54_RS04310 overlap by 4 nucleotides LRK54_RS04310 and LRK54_RS04315 are separated by 108 nucleotides LRK54_RS04315 and LRK54_RS04320 are separated by 5 nucleotides
LRK54_RS04300: LRK54_RS04300 - SDR family oxidoreductase, at 909,122 to 909,955
_RS04300
LRK54_RS04305: LRK54_RS04305 - shikimate kinase, at 910,009 to 910,563
_RS04305
LRK54_RS04310: LRK54_RS04310 - 3-dehydroquinate synthase, at 910,560 to 911,651
_RS04310
LRK54_RS04315: LRK54_RS04315 - WGR domain-containing protein, at 911,760 to 912,020
_RS04315
LRK54_RS04320: LRK54_RS04320 - uroporphyrinogen decarboxylase, at 912,026 to 913,096
_RS04320
Position (kb)
910
911
912 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 909.621 kb on + strand, within LRK54_RS04300 at 909.621 kb on + strand, within LRK54_RS04300 at 909.621 kb on + strand, within LRK54_RS04300 at 909.622 kb on - strand, within LRK54_RS04300 at 909.622 kb on - strand, within LRK54_RS04300 at 909.622 kb on - strand, within LRK54_RS04300 at 909.623 kb on + strand, within LRK54_RS04300 at 909.623 kb on + strand, within LRK54_RS04300 at 909.623 kb on + strand, within LRK54_RS04300 at 909.624 kb on - strand, within LRK54_RS04300 at 909.624 kb on - strand, within LRK54_RS04300 at 909.624 kb on - strand, within LRK54_RS04300 at 909.624 kb on - strand, within LRK54_RS04300 at 909.624 kb on - strand, within LRK54_RS04300 at 909.624 kb on - strand, within LRK54_RS04300 at 909.624 kb on - strand, within LRK54_RS04300 at 909.624 kb on - strand, within LRK54_RS04300 at 909.624 kb on - strand, within LRK54_RS04300 at 909.624 kb on - strand, within LRK54_RS04300 at 909.717 kb on + strand, within LRK54_RS04300 at 909.717 kb on + strand, within LRK54_RS04300 at 909.717 kb on + strand, within LRK54_RS04300 at 909.717 kb on + strand, within LRK54_RS04300 at 909.718 kb on - strand, within LRK54_RS04300 at 909.718 kb on - strand, within LRK54_RS04300 at 909.753 kb on + strand, within LRK54_RS04300 at 909.753 kb on + strand, within LRK54_RS04300 at 909.753 kb on + strand, within LRK54_RS04300 at 909.753 kb on + strand, within LRK54_RS04300 at 909.753 kb on + strand, within LRK54_RS04300 at 909.753 kb on + strand, within LRK54_RS04300 at 909.753 kb on + strand, within LRK54_RS04300 at 909.754 kb on - strand, within LRK54_RS04300 at 909.754 kb on - strand, within LRK54_RS04300 at 909.754 kb on - strand, within LRK54_RS04300 at 909.754 kb on - strand, within LRK54_RS04300 at 909.754 kb on - strand, within LRK54_RS04300 at 909.754 kb on - strand, within LRK54_RS04300 at 909.754 kb on - strand, within LRK54_RS04300 at 909.754 kb on - strand, within LRK54_RS04300 at 909.754 kb on - strand, within LRK54_RS04300 at 909.754 kb on - strand, within LRK54_RS04300 at 909.754 kb on - strand, within LRK54_RS04300 at 909.754 kb on - strand, within LRK54_RS04300 at 909.754 kb on - strand, within LRK54_RS04300 at 909.997 kb on + strand at 909.997 kb on + strand at 909.997 kb on + strand at 909.997 kb on + strand at 909.998 kb on - strand at 909.998 kb on - strand at 909.998 kb on - strand at 909.998 kb on - strand at 910.090 kb on + strand, within LRK54_RS04305 at 910.090 kb on + strand, within LRK54_RS04305 at 910.090 kb on + strand, within LRK54_RS04305 at 910.090 kb on + strand, within LRK54_RS04305 at 910.090 kb on + strand, within LRK54_RS04305 at 910.090 kb on + strand, within LRK54_RS04305 at 910.303 kb on + strand, within LRK54_RS04305 at 910.303 kb on + strand, within LRK54_RS04305 at 910.303 kb on + strand, within LRK54_RS04305 at 911.623 kb on + strand at 911.623 kb on + strand at 911.623 kb on + strand at 911.623 kb on + strand at 911.623 kb on + strand at 911.623 kb on + strand at 911.623 kb on + strand at 911.624 kb on - strand at 911.708 kb on + strand at 911.708 kb on + strand at 911.708 kb on + strand at 911.708 kb on + strand at 911.709 kb on - strand at 911.709 kb on - strand at 911.709 kb on - strand at 911.710 kb on + strand at 911.710 kb on + strand at 911.711 kb on - strand at 911.711 kb on - strand at 911.711 kb on - strand at 911.769 kb on + strand at 911.769 kb on + strand at 911.769 kb on + strand at 911.808 kb on + strand, within LRK54_RS04315 at 911.808 kb on + strand, within LRK54_RS04315 at 911.856 kb on + strand, within LRK54_RS04315 at 912.009 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chromium(III) Chloride Hexahydrate 0.078125 mM remove 909,621 + LRK54_RS04300 0.60 -2.9 909,621 + LRK54_RS04300 0.60 +1.8 909,621 + LRK54_RS04300 0.60 +0.4 909,622 - LRK54_RS04300 0.60 +0.4 909,622 - LRK54_RS04300 0.60 -0.1 909,622 - LRK54_RS04300 0.60 -0.1 909,623 + LRK54_RS04300 0.60 -0.5 909,623 + LRK54_RS04300 0.60 -2.1 909,623 + LRK54_RS04300 0.60 -0.4 909,624 - LRK54_RS04300 0.60 -0.6 909,624 - LRK54_RS04300 0.60 -1.4 909,624 - LRK54_RS04300 0.60 -2.3 909,624 - LRK54_RS04300 0.60 +0.4 909,624 - LRK54_RS04300 0.60 -1.7 909,624 - LRK54_RS04300 0.60 +0.2 909,624 - LRK54_RS04300 0.60 +0.7 909,624 - LRK54_RS04300 0.60 +0.6 909,624 - LRK54_RS04300 0.60 -0.6 909,624 - LRK54_RS04300 0.60 +0.5 909,717 + LRK54_RS04300 0.71 -0.6 909,717 + LRK54_RS04300 0.71 -1.1 909,717 + LRK54_RS04300 0.71 -0.0 909,717 + LRK54_RS04300 0.71 -0.4 909,718 - LRK54_RS04300 0.71 -0.6 909,718 - LRK54_RS04300 0.71 -1.0 909,753 + LRK54_RS04300 0.76 -0.8 909,753 + LRK54_RS04300 0.76 -1.7 909,753 + LRK54_RS04300 0.76 -0.3 909,753 + LRK54_RS04300 0.76 +0.5 909,753 + LRK54_RS04300 0.76 +0.4 909,753 + LRK54_RS04300 0.76 +0.0 909,753 + LRK54_RS04300 0.76 -0.6 909,754 - LRK54_RS04300 0.76 +0.1 909,754 - LRK54_RS04300 0.76 -0.7 909,754 - LRK54_RS04300 0.76 -1.5 909,754 - LRK54_RS04300 0.76 -1.0 909,754 - LRK54_RS04300 0.76 -1.7 909,754 - LRK54_RS04300 0.76 -0.7 909,754 - LRK54_RS04300 0.76 -0.8 909,754 - LRK54_RS04300 0.76 +0.5 909,754 - LRK54_RS04300 0.76 +0.4 909,754 - LRK54_RS04300 0.76 +0.1 909,754 - LRK54_RS04300 0.76 +0.4 909,754 - LRK54_RS04300 0.76 +0.0 909,754 - LRK54_RS04300 0.76 -0.6 909,997 + -1.0 909,997 + -0.4 909,997 + +0.2 909,997 + +0.8 909,998 - +0.8 909,998 - -0.1 909,998 - -0.4 909,998 - -0.9 910,090 + LRK54_RS04305 0.15 -0.6 910,090 + LRK54_RS04305 0.15 +0.8 910,090 + LRK54_RS04305 0.15 -0.2 910,090 + LRK54_RS04305 0.15 -2.5 910,090 + LRK54_RS04305 0.15 -0.7 910,090 + LRK54_RS04305 0.15 -1.7 910,303 + LRK54_RS04305 0.53 -2.0 910,303 + LRK54_RS04305 0.53 -1.0 910,303 + LRK54_RS04305 0.53 -1.0 911,623 + +1.4 911,623 + +0.1 911,623 + -2.9 911,623 + -2.7 911,623 + -0.2 911,623 + -1.0 911,623 + +0.1 911,624 - +0.4 911,708 + -1.8 911,708 + +1.4 911,708 + +0.6 911,708 + -1.2 911,709 - -1.1 911,709 - -1.9 911,709 - -1.3 911,710 + -1.5 911,710 + -0.2 911,711 - +0.1 911,711 - -2.2 911,711 - -0.6 911,769 + -0.6 911,769 + +0.7 911,769 + -0.2 911,808 + LRK54_RS04315 0.18 +0.2 911,808 + LRK54_RS04315 0.18 +0.2 911,856 + LRK54_RS04315 0.37 -0.8 912,009 + -0.3
Or see this region's nucleotide sequence