Experiment: R2A with Chromium(III) Chloride Hexahydrate 0.078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS03665 and LRK54_RS03670 are separated by 278 nucleotides LRK54_RS03670 and LRK54_RS03675 are separated by 139 nucleotides LRK54_RS03675 and LRK54_RS03680 are separated by 81 nucleotides LRK54_RS03680 and LRK54_RS03685 are separated by 54 nucleotides
LRK54_RS03665: LRK54_RS03665 - tRNA-Tyr, at 778,813 to 778,898
_RS03665
LRK54_RS03670: LRK54_RS03670 - acyl-CoA thioesterase, at 779,177 to 779,665
_RS03670
LRK54_RS03675: LRK54_RS03675 - redox-regulated ATPase YchF, at 779,805 to 780,893
_RS03675
LRK54_RS03680: LRK54_RS03680 - aminoacyl-tRNA hydrolase, at 780,975 to 781,556
_RS03680
LRK54_RS03685: LRK54_RS03685 - 50S ribosomal protein L25/general stress protein Ctc, at 781,611 to 782,276
_RS03685
Position (kb)
779
780
781 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 779.075 kb on - strand at 779.075 kb on - strand at 779.520 kb on + strand, within LRK54_RS03670 at 779.520 kb on + strand, within LRK54_RS03670 at 779.520 kb on + strand, within LRK54_RS03670 at 779.520 kb on + strand, within LRK54_RS03670 at 779.520 kb on + strand, within LRK54_RS03670 at 779.520 kb on + strand, within LRK54_RS03670 at 779.520 kb on + strand, within LRK54_RS03670 at 779.521 kb on - strand, within LRK54_RS03670 at 779.521 kb on - strand, within LRK54_RS03670 at 779.521 kb on - strand, within LRK54_RS03670 at 779.521 kb on - strand, within LRK54_RS03670 at 779.521 kb on - strand, within LRK54_RS03670 at 779.521 kb on - strand, within LRK54_RS03670 at 779.521 kb on - strand, within LRK54_RS03670 at 779.521 kb on - strand, within LRK54_RS03670 at 779.521 kb on - strand, within LRK54_RS03670 at 779.521 kb on - strand, within LRK54_RS03670 at 779.521 kb on - strand, within LRK54_RS03670 at 779.550 kb on + strand, within LRK54_RS03670 at 779.551 kb on - strand, within LRK54_RS03670 at 779.551 kb on - strand, within LRK54_RS03670 at 779.656 kb on + strand at 779.692 kb on + strand at 779.692 kb on + strand at 779.692 kb on + strand at 779.692 kb on + strand at 779.692 kb on + strand at 779.692 kb on + strand at 779.692 kb on + strand at 779.692 kb on + strand at 779.692 kb on + strand at 779.692 kb on + strand at 779.692 kb on + strand at 779.692 kb on + strand at 779.692 kb on + strand at 779.693 kb on - strand at 779.693 kb on - strand at 779.693 kb on - strand at 779.693 kb on - strand at 779.693 kb on - strand at 779.693 kb on - strand at 779.693 kb on - strand at 779.703 kb on + strand at 779.703 kb on + strand at 779.703 kb on + strand at 779.703 kb on + strand at 779.703 kb on + strand at 779.703 kb on + strand at 779.703 kb on + strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.704 kb on - strand at 779.851 kb on + strand at 779.852 kb on - strand at 779.852 kb on - strand at 779.852 kb on - strand at 779.852 kb on - strand at 779.852 kb on - strand at 779.908 kb on + strand at 779.909 kb on - strand at 780.052 kb on + strand, within LRK54_RS03675 at 780.052 kb on + strand, within LRK54_RS03675 at 780.077 kb on - strand, within LRK54_RS03675 at 780.189 kb on + strand, within LRK54_RS03675 at 780.286 kb on + strand, within LRK54_RS03675 at 780.286 kb on + strand, within LRK54_RS03675 at 780.286 kb on + strand, within LRK54_RS03675 at 780.286 kb on + strand, within LRK54_RS03675 at 780.287 kb on - strand, within LRK54_RS03675 at 780.287 kb on - strand, within LRK54_RS03675 at 780.969 kb on + strand at 780.969 kb on + strand at 780.970 kb on - strand at 780.970 kb on - strand at 780.970 kb on - strand at 780.976 kb on + strand at 780.976 kb on + strand at 780.976 kb on + strand at 780.976 kb on + strand at 780.977 kb on - strand at 780.977 kb on - strand at 780.977 kb on - strand at 780.977 kb on - strand at 780.977 kb on - strand at 780.989 kb on + strand at 780.989 kb on + strand at 780.989 kb on + strand at 780.989 kb on + strand at 780.990 kb on - strand at 780.990 kb on - strand at 780.990 kb on - strand at 780.990 kb on - strand at 780.990 kb on - strand at 780.990 kb on - strand at 780.990 kb on - strand at 780.990 kb on - strand at 780.990 kb on - strand at 780.990 kb on - strand at 780.990 kb on - strand at 780.990 kb on - strand at 780.990 kb on - strand at 780.990 kb on - strand at 780.990 kb on - strand at 780.990 kb on - strand at 780.990 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chromium(III) Chloride Hexahydrate 0.078125 mM remove 779,075 - +0.4 779,075 - -0.4 779,520 + LRK54_RS03670 0.70 +0.9 779,520 + LRK54_RS03670 0.70 -0.5 779,520 + LRK54_RS03670 0.70 +0.4 779,520 + LRK54_RS03670 0.70 -0.6 779,520 + LRK54_RS03670 0.70 -1.7 779,520 + LRK54_RS03670 0.70 -0.8 779,520 + LRK54_RS03670 0.70 +0.4 779,521 - LRK54_RS03670 0.70 -1.6 779,521 - LRK54_RS03670 0.70 +0.2 779,521 - LRK54_RS03670 0.70 -1.4 779,521 - LRK54_RS03670 0.70 -0.3 779,521 - LRK54_RS03670 0.70 -0.7 779,521 - LRK54_RS03670 0.70 -0.8 779,521 - LRK54_RS03670 0.70 +0.9 779,521 - LRK54_RS03670 0.70 +0.5 779,521 - LRK54_RS03670 0.70 +0.7 779,521 - LRK54_RS03670 0.70 -0.3 779,521 - LRK54_RS03670 0.70 +1.0 779,550 + LRK54_RS03670 0.76 -1.6 779,551 - LRK54_RS03670 0.76 -0.7 779,551 - LRK54_RS03670 0.76 -0.2 779,656 + -0.6 779,692 + +0.4 779,692 + -0.6 779,692 + -1.0 779,692 + +0.2 779,692 + -1.9 779,692 + +0.9 779,692 + -0.7 779,692 + +1.4 779,692 + -0.4 779,692 + -0.5 779,692 + +1.1 779,692 + -1.1 779,692 + -0.4 779,693 - +0.6 779,693 - +1.0 779,693 - -0.6 779,693 - +0.5 779,693 - +1.2 779,693 - +0.9 779,693 - +0.4 779,703 + +1.4 779,703 + -2.4 779,703 + -1.9 779,703 + +0.9 779,703 + -2.1 779,703 + +0.1 779,703 + +0.4 779,704 - +1.9 779,704 - +1.7 779,704 - -0.7 779,704 - +1.4 779,704 - -0.3 779,704 - -0.0 779,704 - +0.3 779,704 - -0.6 779,704 - -1.7 779,704 - +0.2 779,704 - -1.3 779,704 - +0.6 779,704 - +1.1 779,704 - -0.9 779,704 - -1.0 779,704 - +0.4 779,704 - -0.4 779,704 - -0.3 779,704 - -0.0 779,704 - -2.9 779,704 - +2.0 779,704 - +0.3 779,704 - -0.1 779,704 - -1.0 779,704 - -0.1 779,704 - -0.6 779,851 + +0.3 779,852 - +1.4 779,852 - +0.1 779,852 - +0.4 779,852 - -1.5 779,852 - +2.1 779,908 + +0.9 779,909 - -0.9 780,052 + LRK54_RS03675 0.23 -1.0 780,052 + LRK54_RS03675 0.23 +1.1 780,077 - LRK54_RS03675 0.25 +1.4 780,189 + LRK54_RS03675 0.35 -1.3 780,286 + LRK54_RS03675 0.44 -0.4 780,286 + LRK54_RS03675 0.44 -0.4 780,286 + LRK54_RS03675 0.44 +0.7 780,286 + LRK54_RS03675 0.44 -0.9 780,287 - LRK54_RS03675 0.44 -0.4 780,287 - LRK54_RS03675 0.44 +0.4 780,969 + -1.2 780,969 + +1.1 780,970 - -3.1 780,970 - -0.1 780,970 - -1.1 780,976 + -0.1 780,976 + -1.7 780,976 + +1.4 780,976 + -0.2 780,977 - -0.2 780,977 - +1.0 780,977 - +0.0 780,977 - +1.3 780,977 - +0.7 780,989 + -0.7 780,989 + -0.1 780,989 + +0.4 780,989 + +3.0 780,990 - -0.1 780,990 - +1.1 780,990 - -0.6 780,990 - +0.4 780,990 - -1.3 780,990 - +3.3 780,990 - -0.9 780,990 - +0.8 780,990 - -0.6 780,990 - +0.9 780,990 - -0.7 780,990 - -1.3 780,990 - -1.6 780,990 - +0.7 780,990 - +0.3 780,990 - +0.1 780,990 - -0.3
Or see this region's nucleotide sequence