Experiment: R2A with Chromium(III) Chloride Hexahydrate 0.078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS02860 and LRK54_RS02865 are separated by 32 nucleotides LRK54_RS02865 and LRK54_RS02870 are separated by 178 nucleotides LRK54_RS02870 and LRK54_RS02875 overlap by 4 nucleotides
LRK54_RS02860: LRK54_RS02860 - type II toxin-antitoxin system HicB family antitoxin, at 613,392 to 613,811
_RS02860
LRK54_RS02865: LRK54_RS02865 - type II toxin-antitoxin system HicA family toxin, at 613,844 to 614,020
_RS02865
LRK54_RS02870: LRK54_RS02870 - DUF502 domain-containing protein, at 614,199 to 614,876
_RS02870
LRK54_RS02875: LRK54_RS02875 - M14 family metallopeptidase, at 614,873 to 616,705
_RS02875
Position (kb) 614
615 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 613.323 kb on + strand at 613.323 kb on + strand at 613.323 kb on + strand at 613.323 kb on + strand at 613.323 kb on + strand at 613.323 kb on + strand at 613.323 kb on + strand at 613.323 kb on + strand at 613.323 kb on + strand at 613.323 kb on + strand at 613.323 kb on + strand at 613.323 kb on + strand at 613.324 kb on - strand at 613.324 kb on - strand at 613.324 kb on - strand at 613.324 kb on - strand at 613.324 kb on - strand at 613.324 kb on - strand at 613.324 kb on - strand at 613.324 kb on - strand at 613.324 kb on - strand at 613.324 kb on - strand at 613.324 kb on - strand at 613.324 kb on - strand at 613.393 kb on + strand at 613.393 kb on + strand at 613.394 kb on - strand at 613.394 kb on - strand at 613.394 kb on - strand at 613.394 kb on - strand at 613.567 kb on + strand, within LRK54_RS02860 at 613.841 kb on + strand at 613.841 kb on + strand at 613.841 kb on + strand at 613.842 kb on - strand at 613.842 kb on - strand at 613.935 kb on + strand, within LRK54_RS02865 at 613.936 kb on - strand, within LRK54_RS02865 at 614.013 kb on + strand at 614.013 kb on + strand at 614.013 kb on + strand at 614.014 kb on - strand at 614.014 kb on - strand at 614.014 kb on - strand at 614.014 kb on - strand at 614.014 kb on - strand at 614.014 kb on - strand at 614.052 kb on + strand at 614.052 kb on + strand at 614.052 kb on + strand at 614.052 kb on + strand at 614.053 kb on - strand at 614.053 kb on - strand at 614.053 kb on - strand at 614.053 kb on - strand at 614.134 kb on + strand at 614.134 kb on + strand at 614.134 kb on + strand at 614.134 kb on + strand at 614.134 kb on + strand at 614.135 kb on - strand at 614.135 kb on - strand at 614.135 kb on - strand at 614.135 kb on - strand at 614.135 kb on - strand at 614.135 kb on - strand at 614.164 kb on + strand at 614.164 kb on + strand at 614.164 kb on + strand at 614.164 kb on + strand at 614.164 kb on + strand at 614.165 kb on - strand at 614.165 kb on - strand at 614.165 kb on - strand at 614.165 kb on - strand at 614.165 kb on - strand at 614.165 kb on - strand at 614.165 kb on - strand at 614.223 kb on + strand at 614.224 kb on - strand at 614.364 kb on - strand, within LRK54_RS02870 at 614.364 kb on - strand, within LRK54_RS02870 at 614.364 kb on - strand, within LRK54_RS02870 at 614.433 kb on + strand, within LRK54_RS02870 at 614.433 kb on + strand, within LRK54_RS02870 at 614.433 kb on + strand, within LRK54_RS02870 at 614.433 kb on + strand, within LRK54_RS02870 at 614.433 kb on + strand, within LRK54_RS02870 at 614.433 kb on + strand, within LRK54_RS02870 at 614.433 kb on + strand, within LRK54_RS02870 at 614.433 kb on + strand, within LRK54_RS02870 at 614.434 kb on - strand, within LRK54_RS02870 at 614.434 kb on - strand, within LRK54_RS02870 at 614.434 kb on - strand, within LRK54_RS02870 at 614.529 kb on + strand, within LRK54_RS02870 at 614.529 kb on + strand, within LRK54_RS02870 at 614.529 kb on + strand, within LRK54_RS02870 at 614.529 kb on + strand, within LRK54_RS02870 at 614.529 kb on + strand, within LRK54_RS02870 at 614.529 kb on + strand, within LRK54_RS02870 at 614.529 kb on + strand, within LRK54_RS02870 at 614.529 kb on + strand, within LRK54_RS02870 at 614.529 kb on + strand, within LRK54_RS02870 at 614.530 kb on - strand, within LRK54_RS02870 at 614.530 kb on - strand, within LRK54_RS02870 at 614.558 kb on - strand, within LRK54_RS02870 at 614.721 kb on + strand, within LRK54_RS02870 at 615.002 kb on + strand at 615.002 kb on + strand at 615.002 kb on + strand at 615.003 kb on - strand at 615.003 kb on - strand at 615.003 kb on - strand at 615.020 kb on + strand at 615.020 kb on + strand at 615.021 kb on - strand at 615.021 kb on - strand at 615.293 kb on + strand, within LRK54_RS02875 at 615.293 kb on + strand, within LRK54_RS02875 at 615.293 kb on + strand, within LRK54_RS02875 at 615.293 kb on + strand, within LRK54_RS02875 at 615.293 kb on + strand, within LRK54_RS02875 at 615.293 kb on + strand, within LRK54_RS02875 at 615.293 kb on + strand, within LRK54_RS02875 at 615.293 kb on + strand, within LRK54_RS02875 at 615.294 kb on - strand, within LRK54_RS02875 at 615.294 kb on - strand, within LRK54_RS02875 at 615.294 kb on - strand, within LRK54_RS02875 at 615.294 kb on - strand, within LRK54_RS02875 at 615.294 kb on - strand, within LRK54_RS02875 at 615.294 kb on - strand, within LRK54_RS02875 at 615.294 kb on - strand, within LRK54_RS02875 at 615.294 kb on - strand, within LRK54_RS02875 at 615.294 kb on - strand, within LRK54_RS02875 at 615.294 kb on - strand, within LRK54_RS02875 at 615.294 kb on - strand, within LRK54_RS02875 at 615.294 kb on - strand, within LRK54_RS02875 at 615.294 kb on - strand, within LRK54_RS02875 at 615.395 kb on + strand, within LRK54_RS02875 at 615.395 kb on + strand, within LRK54_RS02875 at 615.395 kb on + strand, within LRK54_RS02875 at 615.395 kb on + strand, within LRK54_RS02875 at 615.395 kb on + strand, within LRK54_RS02875 at 615.395 kb on + strand, within LRK54_RS02875 at 615.396 kb on - strand, within LRK54_RS02875 at 615.396 kb on - strand, within LRK54_RS02875 at 615.396 kb on - strand, within LRK54_RS02875 at 615.396 kb on - strand, within LRK54_RS02875 at 615.396 kb on - strand, within LRK54_RS02875 at 615.396 kb on - strand, within LRK54_RS02875 at 615.396 kb on - strand, within LRK54_RS02875 at 615.416 kb on + strand, within LRK54_RS02875 at 615.416 kb on + strand, within LRK54_RS02875 at 615.416 kb on + strand, within LRK54_RS02875 at 615.417 kb on - strand, within LRK54_RS02875 at 615.417 kb on - strand, within LRK54_RS02875 at 615.417 kb on - strand, within LRK54_RS02875 at 615.417 kb on - strand, within LRK54_RS02875 at 615.417 kb on - strand, within LRK54_RS02875 at 615.518 kb on + strand, within LRK54_RS02875 at 615.519 kb on - strand, within LRK54_RS02875 at 615.519 kb on - strand, within LRK54_RS02875 at 615.519 kb on - strand, within LRK54_RS02875 at 615.519 kb on - strand, within LRK54_RS02875 at 615.519 kb on - strand, within LRK54_RS02875 at 615.519 kb on - strand, within LRK54_RS02875 at 615.524 kb on + strand, within LRK54_RS02875 at 615.524 kb on + strand, within LRK54_RS02875 at 615.524 kb on + strand, within LRK54_RS02875 at 615.524 kb on + strand, within LRK54_RS02875 at 615.524 kb on + strand, within LRK54_RS02875 at 615.524 kb on + strand, within LRK54_RS02875 at 615.525 kb on - strand, within LRK54_RS02875 at 615.525 kb on - strand, within LRK54_RS02875 at 615.525 kb on - strand, within LRK54_RS02875 at 615.525 kb on - strand, within LRK54_RS02875 at 615.539 kb on + strand, within LRK54_RS02875 at 615.539 kb on + strand, within LRK54_RS02875 at 615.539 kb on + strand, within LRK54_RS02875 at 615.539 kb on + strand, within LRK54_RS02875 at 615.539 kb on + strand, within LRK54_RS02875 at 615.539 kb on + strand, within LRK54_RS02875 at 615.540 kb on - strand, within LRK54_RS02875 at 615.540 kb on - strand, within LRK54_RS02875 at 615.540 kb on - strand, within LRK54_RS02875 at 615.620 kb on + strand, within LRK54_RS02875 at 615.620 kb on + strand, within LRK54_RS02875 at 615.620 kb on + strand, within LRK54_RS02875 at 615.650 kb on + strand, within LRK54_RS02875 at 615.650 kb on + strand, within LRK54_RS02875 at 615.650 kb on + strand, within LRK54_RS02875 at 615.650 kb on + strand, within LRK54_RS02875 at 615.650 kb on + strand, within LRK54_RS02875 at 615.650 kb on + strand, within LRK54_RS02875 at 615.651 kb on - strand, within LRK54_RS02875 at 615.651 kb on - strand, within LRK54_RS02875 at 615.651 kb on - strand, within LRK54_RS02875 at 615.651 kb on - strand, within LRK54_RS02875 at 615.651 kb on - strand, within LRK54_RS02875 at 615.651 kb on - strand, within LRK54_RS02875 at 615.729 kb on - strand, within LRK54_RS02875 at 615.785 kb on + strand, within LRK54_RS02875 at 615.785 kb on + strand, within LRK54_RS02875 at 615.809 kb on + strand, within LRK54_RS02875 at 615.809 kb on + strand, within LRK54_RS02875 at 615.809 kb on + strand, within LRK54_RS02875 at 615.809 kb on + strand, within LRK54_RS02875 at 615.809 kb on + strand, within LRK54_RS02875 at 615.809 kb on + strand, within LRK54_RS02875 at 615.809 kb on + strand, within LRK54_RS02875 at 615.810 kb on - strand, within LRK54_RS02875 at 615.810 kb on - strand, within LRK54_RS02875 at 615.810 kb on - strand, within LRK54_RS02875 at 615.810 kb on - strand, within LRK54_RS02875 at 615.810 kb on - strand, within LRK54_RS02875 at 615.810 kb on - strand, within LRK54_RS02875 at 615.810 kb on - strand, within LRK54_RS02875 at 615.810 kb on - strand, within LRK54_RS02875 at 615.810 kb on - strand, within LRK54_RS02875 at 615.810 kb on - strand, within LRK54_RS02875 at 615.810 kb on - strand, within LRK54_RS02875 at 615.861 kb on + strand, within LRK54_RS02875 at 615.861 kb on + strand, within LRK54_RS02875 at 615.861 kb on + strand, within LRK54_RS02875
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chromium(III) Chloride Hexahydrate 0.078125 mM remove 613,323 + +0.3 613,323 + -0.9 613,323 + +0.6 613,323 + +0.4 613,323 + -0.5 613,323 + -1.0 613,323 + -0.5 613,323 + +0.5 613,323 + -0.4 613,323 + +0.9 613,323 + +0.1 613,323 + +0.6 613,324 - -0.6 613,324 - -0.5 613,324 - +2.2 613,324 - +0.1 613,324 - -1.3 613,324 - +0.2 613,324 - -0.5 613,324 - -0.2 613,324 - +0.5 613,324 - +0.2 613,324 - -1.1 613,324 - +0.4 613,393 + -1.4 613,393 + +0.7 613,394 - +0.3 613,394 - +0.4 613,394 - -0.3 613,394 - +0.6 613,567 + LRK54_RS02860 0.42 +0.3 613,841 + -0.6 613,841 + -0.6 613,841 + -0.0 613,842 - -2.6 613,842 - +0.4 613,935 + LRK54_RS02865 0.51 -0.4 613,936 - LRK54_RS02865 0.52 -1.9 614,013 + -0.5 614,013 + -0.2 614,013 + +0.1 614,014 - +0.8 614,014 - +0.1 614,014 - -0.6 614,014 - -1.5 614,014 - -0.2 614,014 - -2.6 614,052 + -0.4 614,052 + +0.4 614,052 + +0.0 614,052 + -0.1 614,053 - +0.4 614,053 - +0.9 614,053 - +2.4 614,053 - +0.7 614,134 + -0.6 614,134 + +1.9 614,134 + -1.0 614,134 + +2.3 614,134 + -1.3 614,135 - -1.6 614,135 - +0.2 614,135 - +0.4 614,135 - -0.5 614,135 - +0.4 614,135 - -2.1 614,164 + +0.5 614,164 + +0.1 614,164 + -0.8 614,164 + +0.7 614,164 + +0.3 614,165 - +1.6 614,165 - +0.2 614,165 - +0.4 614,165 - -0.0 614,165 - -0.5 614,165 - -2.9 614,165 - -0.7 614,223 + -1.4 614,224 - -0.4 614,364 - LRK54_RS02870 0.24 -0.7 614,364 - LRK54_RS02870 0.24 -2.6 614,364 - LRK54_RS02870 0.24 -0.5 614,433 + LRK54_RS02870 0.35 -0.4 614,433 + LRK54_RS02870 0.35 +0.1 614,433 + LRK54_RS02870 0.35 -1.5 614,433 + LRK54_RS02870 0.35 -0.3 614,433 + LRK54_RS02870 0.35 -0.9 614,433 + LRK54_RS02870 0.35 +0.4 614,433 + LRK54_RS02870 0.35 -0.7 614,433 + LRK54_RS02870 0.35 -1.5 614,434 - LRK54_RS02870 0.35 -0.9 614,434 - LRK54_RS02870 0.35 -1.8 614,434 - LRK54_RS02870 0.35 -1.5 614,529 + LRK54_RS02870 0.49 +0.6 614,529 + LRK54_RS02870 0.49 -2.0 614,529 + LRK54_RS02870 0.49 -0.7 614,529 + LRK54_RS02870 0.49 +0.3 614,529 + LRK54_RS02870 0.49 -0.7 614,529 + LRK54_RS02870 0.49 +0.4 614,529 + LRK54_RS02870 0.49 -0.4 614,529 + LRK54_RS02870 0.49 -0.4 614,529 + LRK54_RS02870 0.49 -0.4 614,530 - LRK54_RS02870 0.49 +1.4 614,530 - LRK54_RS02870 0.49 +0.2 614,558 - LRK54_RS02870 0.53 -1.1 614,721 + LRK54_RS02870 0.77 -0.9 615,002 + -1.3 615,002 + -1.3 615,002 + -0.4 615,003 - -0.6 615,003 - -1.2 615,003 - -0.4 615,020 + +0.2 615,020 + +0.1 615,021 - -0.1 615,021 - -0.2 615,293 + LRK54_RS02875 0.23 +0.4 615,293 + LRK54_RS02875 0.23 -1.5 615,293 + LRK54_RS02875 0.23 -0.5 615,293 + LRK54_RS02875 0.23 +0.5 615,293 + LRK54_RS02875 0.23 -1.7 615,293 + LRK54_RS02875 0.23 -1.0 615,293 + LRK54_RS02875 0.23 +0.1 615,293 + LRK54_RS02875 0.23 +0.9 615,294 - LRK54_RS02875 0.23 +0.9 615,294 - LRK54_RS02875 0.23 +1.2 615,294 - LRK54_RS02875 0.23 +0.3 615,294 - LRK54_RS02875 0.23 +0.3 615,294 - LRK54_RS02875 0.23 +0.5 615,294 - LRK54_RS02875 0.23 -0.1 615,294 - LRK54_RS02875 0.23 -0.7 615,294 - LRK54_RS02875 0.23 +0.7 615,294 - LRK54_RS02875 0.23 -0.4 615,294 - LRK54_RS02875 0.23 -2.3 615,294 - LRK54_RS02875 0.23 -0.7 615,294 - LRK54_RS02875 0.23 -0.2 615,294 - LRK54_RS02875 0.23 -0.2 615,395 + LRK54_RS02875 0.28 -0.6 615,395 + LRK54_RS02875 0.28 +0.1 615,395 + LRK54_RS02875 0.28 -2.5 615,395 + LRK54_RS02875 0.28 -1.6 615,395 + LRK54_RS02875 0.28 -0.7 615,395 + LRK54_RS02875 0.28 +0.1 615,396 - LRK54_RS02875 0.29 -0.8 615,396 - LRK54_RS02875 0.29 -1.5 615,396 - LRK54_RS02875 0.29 -0.5 615,396 - LRK54_RS02875 0.29 -1.1 615,396 - LRK54_RS02875 0.29 -0.6 615,396 - LRK54_RS02875 0.29 -0.5 615,396 - LRK54_RS02875 0.29 -0.4 615,416 + LRK54_RS02875 0.30 -1.0 615,416 + LRK54_RS02875 0.30 +0.9 615,416 + LRK54_RS02875 0.30 +1.4 615,417 - LRK54_RS02875 0.30 -0.8 615,417 - LRK54_RS02875 0.30 -0.7 615,417 - LRK54_RS02875 0.30 +0.1 615,417 - LRK54_RS02875 0.30 -0.1 615,417 - LRK54_RS02875 0.30 -0.0 615,518 + LRK54_RS02875 0.35 -1.8 615,519 - LRK54_RS02875 0.35 +0.6 615,519 - LRK54_RS02875 0.35 -2.3 615,519 - LRK54_RS02875 0.35 +0.5 615,519 - LRK54_RS02875 0.35 +0.8 615,519 - LRK54_RS02875 0.35 -1.6 615,519 - LRK54_RS02875 0.35 +0.2 615,524 + LRK54_RS02875 0.36 -1.9 615,524 + LRK54_RS02875 0.36 -0.6 615,524 + LRK54_RS02875 0.36 -0.2 615,524 + LRK54_RS02875 0.36 -1.3 615,524 + LRK54_RS02875 0.36 +0.2 615,524 + LRK54_RS02875 0.36 +0.2 615,525 - LRK54_RS02875 0.36 -1.0 615,525 - LRK54_RS02875 0.36 -0.4 615,525 - LRK54_RS02875 0.36 -1.7 615,525 - LRK54_RS02875 0.36 +0.1 615,539 + LRK54_RS02875 0.36 +0.7 615,539 + LRK54_RS02875 0.36 -0.4 615,539 + LRK54_RS02875 0.36 +0.9 615,539 + LRK54_RS02875 0.36 -0.5 615,539 + LRK54_RS02875 0.36 -0.1 615,539 + LRK54_RS02875 0.36 -0.3 615,540 - LRK54_RS02875 0.36 -0.3 615,540 - LRK54_RS02875 0.36 -1.4 615,540 - LRK54_RS02875 0.36 -1.5 615,620 + LRK54_RS02875 0.41 -0.1 615,620 + LRK54_RS02875 0.41 -0.4 615,620 + LRK54_RS02875 0.41 -0.4 615,650 + LRK54_RS02875 0.42 -0.2 615,650 + LRK54_RS02875 0.42 -0.6 615,650 + LRK54_RS02875 0.42 -1.1 615,650 + LRK54_RS02875 0.42 +0.7 615,650 + LRK54_RS02875 0.42 -1.3 615,650 + LRK54_RS02875 0.42 -0.9 615,651 - LRK54_RS02875 0.42 -0.6 615,651 - LRK54_RS02875 0.42 -0.1 615,651 - LRK54_RS02875 0.42 -0.6 615,651 - LRK54_RS02875 0.42 +0.1 615,651 - LRK54_RS02875 0.42 -1.8 615,651 - LRK54_RS02875 0.42 +1.6 615,729 - LRK54_RS02875 0.47 +1.1 615,785 + LRK54_RS02875 0.50 -0.6 615,785 + LRK54_RS02875 0.50 +0.1 615,809 + LRK54_RS02875 0.51 -0.4 615,809 + LRK54_RS02875 0.51 +0.1 615,809 + LRK54_RS02875 0.51 +0.1 615,809 + LRK54_RS02875 0.51 -0.1 615,809 + LRK54_RS02875 0.51 -0.0 615,809 + LRK54_RS02875 0.51 -0.5 615,809 + LRK54_RS02875 0.51 +1.1 615,810 - LRK54_RS02875 0.51 +1.1 615,810 - LRK54_RS02875 0.51 +1.9 615,810 - LRK54_RS02875 0.51 +0.4 615,810 - LRK54_RS02875 0.51 -0.7 615,810 - LRK54_RS02875 0.51 -1.3 615,810 - LRK54_RS02875 0.51 +0.9 615,810 - LRK54_RS02875 0.51 +0.1 615,810 - LRK54_RS02875 0.51 -1.4 615,810 - LRK54_RS02875 0.51 +0.4 615,810 - LRK54_RS02875 0.51 +0.7 615,810 - LRK54_RS02875 0.51 -0.3 615,861 + LRK54_RS02875 0.54 +0.3 615,861 + LRK54_RS02875 0.54 +0.7 615,861 + LRK54_RS02875 0.54 +0.2
Or see this region's nucleotide sequence