Experiment: R2A with Chromium(III) Chloride Hexahydrate 0.078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS01580 and LRK54_RS01585 overlap by 7 nucleotides LRK54_RS01585 and LRK54_RS01590 overlap by 4 nucleotides LRK54_RS01590 and LRK54_RS01595 are separated by 109 nucleotides
LRK54_RS01580: LRK54_RS01580 - imidazole glycerol phosphate synthase subunit HisF, at 341,396 to 342,169
_RS01580
LRK54_RS01585: LRK54_RS01585 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 342,163 to 342,891
_RS01585
LRK54_RS01590: LRK54_RS01590 - imidazole glycerol phosphate synthase subunit HisH, at 342,888 to 343,478
_RS01590
LRK54_RS01595: LRK54_RS01595 - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 343,588 to 344,652
_RS01595
Position (kb) 342
343
344 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.022 kb on - strand, within LRK54_RS01580 at 342.022 kb on - strand, within LRK54_RS01580 at 342.022 kb on - strand, within LRK54_RS01580 at 342.022 kb on - strand, within LRK54_RS01580 at 342.022 kb on - strand, within LRK54_RS01580 at 342.329 kb on + strand, within LRK54_RS01585 at 342.410 kb on + strand, within LRK54_RS01585 at 342.410 kb on + strand, within LRK54_RS01585 at 342.411 kb on - strand, within LRK54_RS01585 at 342.411 kb on - strand, within LRK54_RS01585 at 342.530 kb on + strand, within LRK54_RS01585 at 342.530 kb on + strand, within LRK54_RS01585 at 342.531 kb on - strand, within LRK54_RS01585 at 342.640 kb on + strand, within LRK54_RS01585 at 342.640 kb on + strand, within LRK54_RS01585 at 342.640 kb on + strand, within LRK54_RS01585 at 342.770 kb on + strand, within LRK54_RS01585 at 342.771 kb on - strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.822 kb on - strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 343.003 kb on + strand, within LRK54_RS01590 at 343.003 kb on + strand, within LRK54_RS01590 at 343.003 kb on + strand, within LRK54_RS01590 at 343.003 kb on + strand, within LRK54_RS01590 at 343.003 kb on + strand, within LRK54_RS01590 at 343.004 kb on - strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.060 kb on + strand, within LRK54_RS01590 at 343.060 kb on + strand, within LRK54_RS01590 at 343.060 kb on + strand, within LRK54_RS01590 at 343.060 kb on + strand, within LRK54_RS01590 at 343.060 kb on + strand, within LRK54_RS01590 at 343.061 kb on - strand, within LRK54_RS01590 at 343.061 kb on - strand, within LRK54_RS01590 at 343.061 kb on - strand, within LRK54_RS01590 at 343.061 kb on - strand, within LRK54_RS01590 at 343.061 kb on - strand, within LRK54_RS01590 at 343.391 kb on + strand, within LRK54_RS01590 at 343.426 kb on + strand at 343.426 kb on + strand at 343.426 kb on + strand at 343.426 kb on + strand at 343.426 kb on + strand at 343.427 kb on - strand at 343.427 kb on - strand at 343.431 kb on + strand at 343.431 kb on + strand at 343.431 kb on + strand at 343.443 kb on - strand at 343.482 kb on + strand at 343.482 kb on + strand at 343.482 kb on + strand at 343.482 kb on + strand at 343.482 kb on + strand at 343.482 kb on + strand at 343.482 kb on + strand at 343.482 kb on + strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.483 kb on - strand at 343.540 kb on + strand at 343.540 kb on + strand at 343.540 kb on + strand at 343.540 kb on + strand at 343.541 kb on - strand at 343.541 kb on - strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.589 kb on + strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.590 kb on - strand at 343.606 kb on - strand at 343.823 kb on + strand, within LRK54_RS01595 at 343.823 kb on + strand, within LRK54_RS01595 at 343.823 kb on + strand, within LRK54_RS01595 at 343.823 kb on + strand, within LRK54_RS01595 at 343.823 kb on + strand, within LRK54_RS01595 at 343.823 kb on + strand, within LRK54_RS01595 at 343.824 kb on - strand, within LRK54_RS01595 at 344.315 kb on + strand, within LRK54_RS01595 at 344.315 kb on + strand, within LRK54_RS01595 at 344.315 kb on + strand, within LRK54_RS01595 at 344.315 kb on + strand, within LRK54_RS01595 at 344.315 kb on + strand, within LRK54_RS01595 at 344.316 kb on - strand, within LRK54_RS01595 at 344.316 kb on - strand, within LRK54_RS01595 at 344.316 kb on - strand, within LRK54_RS01595 at 344.316 kb on - strand, within LRK54_RS01595
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chromium(III) Chloride Hexahydrate 0.078125 mM remove 342,021 + LRK54_RS01580 0.81 -1.5 342,021 + LRK54_RS01580 0.81 -1.2 342,021 + LRK54_RS01580 0.81 -2.2 342,021 + LRK54_RS01580 0.81 -2.5 342,021 + LRK54_RS01580 0.81 -1.6 342,021 + LRK54_RS01580 0.81 -3.3 342,021 + LRK54_RS01580 0.81 -0.8 342,021 + LRK54_RS01580 0.81 +2.0 342,021 + LRK54_RS01580 0.81 -2.2 342,021 + LRK54_RS01580 0.81 +0.0 342,021 + LRK54_RS01580 0.81 +0.7 342,021 + LRK54_RS01580 0.81 -1.2 342,021 + LRK54_RS01580 0.81 -1.0 342,021 + LRK54_RS01580 0.81 -0.6 342,022 - LRK54_RS01580 0.81 -1.3 342,022 - LRK54_RS01580 0.81 -1.6 342,022 - LRK54_RS01580 0.81 -1.5 342,022 - LRK54_RS01580 0.81 -2.2 342,022 - LRK54_RS01580 0.81 -1.2 342,329 + LRK54_RS01585 0.23 -1.0 342,410 + LRK54_RS01585 0.34 -0.7 342,410 + LRK54_RS01585 0.34 -2.5 342,411 - LRK54_RS01585 0.34 -1.8 342,411 - LRK54_RS01585 0.34 -0.6 342,530 + LRK54_RS01585 0.50 -1.7 342,530 + LRK54_RS01585 0.50 +1.4 342,531 - LRK54_RS01585 0.50 -1.3 342,640 + LRK54_RS01585 0.65 -1.6 342,640 + LRK54_RS01585 0.65 -1.0 342,640 + LRK54_RS01585 0.65 -0.0 342,770 + LRK54_RS01585 0.83 +0.6 342,771 - LRK54_RS01585 0.83 +1.2 342,803 + LRK54_RS01585 0.88 -0.9 342,803 + LRK54_RS01585 0.88 +0.1 342,803 + LRK54_RS01585 0.88 -1.9 342,803 + LRK54_RS01585 0.88 +0.1 342,803 + LRK54_RS01585 0.88 -0.9 342,803 + LRK54_RS01585 0.88 -2.6 342,803 + LRK54_RS01585 0.88 -0.4 342,803 + LRK54_RS01585 0.88 -1.3 342,803 + LRK54_RS01585 0.88 -1.7 342,803 + LRK54_RS01585 0.88 -0.5 342,803 + LRK54_RS01585 0.88 -1.9 342,803 + LRK54_RS01585 0.88 -1.0 342,803 + LRK54_RS01585 0.88 -2.3 342,803 + LRK54_RS01585 0.88 +0.8 342,803 + LRK54_RS01585 0.88 +1.0 342,803 + LRK54_RS01585 0.88 -2.2 342,804 - LRK54_RS01585 0.88 -0.4 342,804 - LRK54_RS01585 0.88 -2.3 342,804 - LRK54_RS01585 0.88 +0.3 342,804 - LRK54_RS01585 0.88 +0.1 342,804 - LRK54_RS01585 0.88 +0.1 342,804 - LRK54_RS01585 0.88 -1.7 342,804 - LRK54_RS01585 0.88 -3.0 342,804 - LRK54_RS01585 0.88 -1.7 342,804 - LRK54_RS01585 0.88 -0.7 342,804 - LRK54_RS01585 0.88 -0.4 342,822 - -0.9 342,891 + +0.4 342,891 + -0.7 342,891 + +1.1 342,891 + +0.3 342,891 + -0.2 342,891 + -1.3 342,891 + +2.0 342,891 + -1.5 342,891 + -0.8 342,891 + -1.1 342,891 + -0.9 342,891 + -0.6 342,892 - -0.4 342,892 - -0.6 342,892 - +0.3 342,892 - -0.1 342,892 - +0.6 342,892 - +0.1 342,892 - -0.8 342,892 - -1.7 342,892 - -0.2 342,892 - -1.0 342,892 - -1.0 343,003 + LRK54_RS01590 0.19 -3.3 343,003 + LRK54_RS01590 0.19 +1.2 343,003 + LRK54_RS01590 0.19 -0.8 343,003 + LRK54_RS01590 0.19 -0.5 343,003 + LRK54_RS01590 0.19 +1.4 343,004 - LRK54_RS01590 0.20 -0.3 343,045 + LRK54_RS01590 0.27 -0.7 343,045 + LRK54_RS01590 0.27 -1.7 343,045 + LRK54_RS01590 0.27 -2.9 343,045 + LRK54_RS01590 0.27 +0.8 343,045 + LRK54_RS01590 0.27 +0.3 343,045 + LRK54_RS01590 0.27 -2.1 343,045 + LRK54_RS01590 0.27 -0.4 343,045 + LRK54_RS01590 0.27 -0.3 343,045 + LRK54_RS01590 0.27 -0.7 343,045 + LRK54_RS01590 0.27 -0.6 343,045 + LRK54_RS01590 0.27 -0.9 343,045 + LRK54_RS01590 0.27 -0.6 343,045 + LRK54_RS01590 0.27 -1.3 343,045 + LRK54_RS01590 0.27 -1.5 343,046 - LRK54_RS01590 0.27 -0.4 343,046 - LRK54_RS01590 0.27 -0.6 343,046 - LRK54_RS01590 0.27 -2.3 343,046 - LRK54_RS01590 0.27 -0.4 343,046 - LRK54_RS01590 0.27 -1.3 343,046 - LRK54_RS01590 0.27 -2.1 343,046 - LRK54_RS01590 0.27 -0.9 343,046 - LRK54_RS01590 0.27 -1.5 343,046 - LRK54_RS01590 0.27 -0.3 343,046 - LRK54_RS01590 0.27 -2.8 343,046 - LRK54_RS01590 0.27 -1.6 343,046 - LRK54_RS01590 0.27 -1.1 343,046 - LRK54_RS01590 0.27 -2.6 343,046 - LRK54_RS01590 0.27 -2.0 343,046 - LRK54_RS01590 0.27 -1.2 343,046 - LRK54_RS01590 0.27 -3.0 343,046 - LRK54_RS01590 0.27 +1.1 343,046 - LRK54_RS01590 0.27 -1.4 343,046 - LRK54_RS01590 0.27 +0.5 343,046 - LRK54_RS01590 0.27 +0.1 343,046 - LRK54_RS01590 0.27 -1.3 343,046 - LRK54_RS01590 0.27 -0.4 343,046 - LRK54_RS01590 0.27 -2.1 343,060 + LRK54_RS01590 0.29 -0.7 343,060 + LRK54_RS01590 0.29 -1.7 343,060 + LRK54_RS01590 0.29 -0.5 343,060 + LRK54_RS01590 0.29 +2.1 343,060 + LRK54_RS01590 0.29 -0.7 343,061 - LRK54_RS01590 0.29 -0.2 343,061 - LRK54_RS01590 0.29 -1.4 343,061 - LRK54_RS01590 0.29 -3.7 343,061 - LRK54_RS01590 0.29 -1.6 343,061 - LRK54_RS01590 0.29 -1.4 343,391 + LRK54_RS01590 0.85 +0.1 343,426 + -1.5 343,426 + -0.2 343,426 + -0.6 343,426 + +0.1 343,426 + +1.3 343,427 - -0.0 343,427 - +1.1 343,431 + -2.2 343,431 + -1.8 343,431 + -2.5 343,443 - -2.0 343,482 + +0.4 343,482 + -1.5 343,482 + -0.9 343,482 + -1.8 343,482 + -1.6 343,482 + -0.5 343,482 + -0.6 343,482 + -3.1 343,483 - -0.2 343,483 - -0.4 343,483 - -0.9 343,483 - -2.3 343,483 - -0.5 343,483 - -0.2 343,483 - -0.0 343,483 - -2.0 343,483 - +0.1 343,483 - +3.4 343,483 - -1.2 343,540 + -0.2 343,540 + -2.1 343,540 + -0.2 343,540 + -1.0 343,541 - +1.0 343,541 - -0.9 343,589 + -1.4 343,589 + -1.3 343,589 + +0.4 343,589 + +0.2 343,589 + +1.2 343,589 + -1.6 343,589 + +0.8 343,589 + -0.0 343,589 + -0.4 343,589 + -0.5 343,589 + -0.5 343,589 + +0.3 343,589 + +0.5 343,589 + +0.7 343,589 + -0.3 343,589 + -0.6 343,589 + -1.3 343,589 + +0.6 343,589 + -1.4 343,589 + +1.6 343,589 + -0.3 343,589 + +0.1 343,589 + -1.3 343,589 + +1.0 343,590 - -1.6 343,590 - +2.4 343,590 - +1.2 343,590 - -0.4 343,590 - -1.4 343,590 - +2.3 343,590 - -1.2 343,590 - +0.1 343,590 - +1.8 343,590 - +0.4 343,590 - +0.7 343,590 - +0.3 343,590 - -0.3 343,590 - +1.0 343,590 - +1.0 343,590 - +1.2 343,590 - -0.4 343,590 - -1.3 343,590 - +0.9 343,590 - +0.4 343,606 - +0.0 343,823 + LRK54_RS01595 0.22 -0.2 343,823 + LRK54_RS01595 0.22 +0.1 343,823 + LRK54_RS01595 0.22 -0.6 343,823 + LRK54_RS01595 0.22 +0.5 343,823 + LRK54_RS01595 0.22 +0.6 343,823 + LRK54_RS01595 0.22 +0.1 343,824 - LRK54_RS01595 0.22 -3.4 344,315 + LRK54_RS01595 0.68 -1.7 344,315 + LRK54_RS01595 0.68 -1.5 344,315 + LRK54_RS01595 0.68 -1.2 344,315 + LRK54_RS01595 0.68 +0.4 344,315 + LRK54_RS01595 0.68 -1.5 344,316 - LRK54_RS01595 0.68 -0.0 344,316 - LRK54_RS01595 0.68 +1.4 344,316 - LRK54_RS01595 0.68 -1.5 344,316 - LRK54_RS01595 0.68 +0.1
Or see this region's nucleotide sequence