Experiment: R2A with Chromium(III) Chloride Hexahydrate 0.078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS01570 and LRK54_RS01575 are separated by 24 nucleotides LRK54_RS01575 and LRK54_RS01580 are separated by 213 nucleotides LRK54_RS01580 and LRK54_RS01585 overlap by 7 nucleotides LRK54_RS01585 and LRK54_RS01590 overlap by 4 nucleotides
LRK54_RS01570: LRK54_RS01570 - LysR family transcriptional regulator, at 339,677 to 340,528
_RS01570
LRK54_RS01575: LRK54_RS01575 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 340,553 to 341,182
_RS01575
LRK54_RS01580: LRK54_RS01580 - imidazole glycerol phosphate synthase subunit HisF, at 341,396 to 342,169
_RS01580
LRK54_RS01585: LRK54_RS01585 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 342,163 to 342,891
_RS01585
LRK54_RS01590: LRK54_RS01590 - imidazole glycerol phosphate synthase subunit HisH, at 342,888 to 343,478
_RS01590
Position (kb) 341
342
343 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 340.454 kb on + strand at 340.455 kb on - strand at 340.455 kb on - strand at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.770 kb on + strand, within LRK54_RS01575 at 340.771 kb on - strand, within LRK54_RS01575 at 340.771 kb on - strand, within LRK54_RS01575 at 340.771 kb on - strand, within LRK54_RS01575 at 340.771 kb on - strand, within LRK54_RS01575 at 340.771 kb on - strand, within LRK54_RS01575 at 340.771 kb on - strand, within LRK54_RS01575 at 340.771 kb on - strand, within LRK54_RS01575 at 340.771 kb on - strand, within LRK54_RS01575 at 340.771 kb on - strand, within LRK54_RS01575 at 340.771 kb on - strand, within LRK54_RS01575 at 340.771 kb on - strand, within LRK54_RS01575 at 340.771 kb on - strand, within LRK54_RS01575 at 341.073 kb on + strand, within LRK54_RS01575 at 341.073 kb on + strand, within LRK54_RS01575 at 341.073 kb on + strand, within LRK54_RS01575 at 341.074 kb on - strand, within LRK54_RS01575 at 341.074 kb on - strand, within LRK54_RS01575 at 341.074 kb on - strand, within LRK54_RS01575 at 341.074 kb on - strand, within LRK54_RS01575 at 341.187 kb on + strand at 341.187 kb on + strand at 341.187 kb on + strand at 341.188 kb on - strand at 341.188 kb on - strand at 341.188 kb on - strand at 341.433 kb on + strand at 341.433 kb on + strand at 341.433 kb on + strand at 341.433 kb on + strand at 341.433 kb on + strand at 341.433 kb on + strand at 341.433 kb on + strand at 341.433 kb on + strand at 341.433 kb on + strand at 341.434 kb on - strand at 341.434 kb on - strand at 341.434 kb on - strand at 341.434 kb on - strand at 341.610 kb on + strand, within LRK54_RS01580 at 341.739 kb on + strand, within LRK54_RS01580 at 341.739 kb on + strand, within LRK54_RS01580 at 341.739 kb on + strand, within LRK54_RS01580 at 341.739 kb on + strand, within LRK54_RS01580 at 341.740 kb on - strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.021 kb on + strand, within LRK54_RS01580 at 342.022 kb on - strand, within LRK54_RS01580 at 342.022 kb on - strand, within LRK54_RS01580 at 342.022 kb on - strand, within LRK54_RS01580 at 342.022 kb on - strand, within LRK54_RS01580 at 342.022 kb on - strand, within LRK54_RS01580 at 342.329 kb on + strand, within LRK54_RS01585 at 342.410 kb on + strand, within LRK54_RS01585 at 342.410 kb on + strand, within LRK54_RS01585 at 342.411 kb on - strand, within LRK54_RS01585 at 342.411 kb on - strand, within LRK54_RS01585 at 342.530 kb on + strand, within LRK54_RS01585 at 342.530 kb on + strand, within LRK54_RS01585 at 342.531 kb on - strand, within LRK54_RS01585 at 342.640 kb on + strand, within LRK54_RS01585 at 342.640 kb on + strand, within LRK54_RS01585 at 342.640 kb on + strand, within LRK54_RS01585 at 342.770 kb on + strand, within LRK54_RS01585 at 342.771 kb on - strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.803 kb on + strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.804 kb on - strand, within LRK54_RS01585 at 342.822 kb on - strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.891 kb on + strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 342.892 kb on - strand at 343.003 kb on + strand, within LRK54_RS01590 at 343.003 kb on + strand, within LRK54_RS01590 at 343.003 kb on + strand, within LRK54_RS01590 at 343.003 kb on + strand, within LRK54_RS01590 at 343.003 kb on + strand, within LRK54_RS01590 at 343.004 kb on - strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.045 kb on + strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.046 kb on - strand, within LRK54_RS01590 at 343.060 kb on + strand, within LRK54_RS01590 at 343.060 kb on + strand, within LRK54_RS01590 at 343.060 kb on + strand, within LRK54_RS01590 at 343.060 kb on + strand, within LRK54_RS01590 at 343.060 kb on + strand, within LRK54_RS01590 at 343.061 kb on - strand, within LRK54_RS01590 at 343.061 kb on - strand, within LRK54_RS01590 at 343.061 kb on - strand, within LRK54_RS01590 at 343.061 kb on - strand, within LRK54_RS01590 at 343.061 kb on - strand, within LRK54_RS01590
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chromium(III) Chloride Hexahydrate 0.078125 mM remove 340,454 + -0.4 340,455 - -0.1 340,455 - -0.2 340,770 + LRK54_RS01575 0.34 +0.7 340,770 + LRK54_RS01575 0.34 -1.2 340,770 + LRK54_RS01575 0.34 -1.3 340,770 + LRK54_RS01575 0.34 -2.8 340,770 + LRK54_RS01575 0.34 -0.1 340,770 + LRK54_RS01575 0.34 +0.1 340,770 + LRK54_RS01575 0.34 -2.3 340,770 + LRK54_RS01575 0.34 +0.6 340,770 + LRK54_RS01575 0.34 -1.0 340,770 + LRK54_RS01575 0.34 -0.5 340,770 + LRK54_RS01575 0.34 -1.7 340,770 + LRK54_RS01575 0.34 +1.7 340,770 + LRK54_RS01575 0.34 +0.3 340,770 + LRK54_RS01575 0.34 +0.3 340,770 + LRK54_RS01575 0.34 -0.5 340,770 + LRK54_RS01575 0.34 -0.0 340,770 + LRK54_RS01575 0.34 -0.2 340,770 + LRK54_RS01575 0.34 +0.2 340,770 + LRK54_RS01575 0.34 +1.1 340,770 + LRK54_RS01575 0.34 -0.4 340,770 + LRK54_RS01575 0.34 -1.3 340,770 + LRK54_RS01575 0.34 -2.3 340,771 - LRK54_RS01575 0.35 +1.4 340,771 - LRK54_RS01575 0.35 +0.7 340,771 - LRK54_RS01575 0.35 +2.7 340,771 - LRK54_RS01575 0.35 -0.4 340,771 - LRK54_RS01575 0.35 -0.3 340,771 - LRK54_RS01575 0.35 -0.6 340,771 - LRK54_RS01575 0.35 +0.4 340,771 - LRK54_RS01575 0.35 +0.6 340,771 - LRK54_RS01575 0.35 +0.2 340,771 - LRK54_RS01575 0.35 -1.7 340,771 - LRK54_RS01575 0.35 -0.5 340,771 - LRK54_RS01575 0.35 -0.1 341,073 + LRK54_RS01575 0.83 -0.3 341,073 + LRK54_RS01575 0.83 -0.9 341,073 + LRK54_RS01575 0.83 -1.0 341,074 - LRK54_RS01575 0.83 +0.4 341,074 - LRK54_RS01575 0.83 -0.4 341,074 - LRK54_RS01575 0.83 -1.1 341,074 - LRK54_RS01575 0.83 +1.2 341,187 + +0.2 341,187 + -0.5 341,187 + -0.4 341,188 - +0.4 341,188 - -0.4 341,188 - +0.1 341,433 + -1.4 341,433 + +0.1 341,433 + -1.6 341,433 + -0.5 341,433 + +0.1 341,433 + -2.4 341,433 + -0.1 341,433 + +1.4 341,433 + -1.1 341,434 - -1.9 341,434 - -0.6 341,434 - -1.2 341,434 - -1.4 341,610 + LRK54_RS01580 0.28 -2.6 341,739 + LRK54_RS01580 0.44 -0.9 341,739 + LRK54_RS01580 0.44 -0.5 341,739 + LRK54_RS01580 0.44 -0.9 341,739 + LRK54_RS01580 0.44 -3.5 341,740 - LRK54_RS01580 0.44 -2.2 342,021 + LRK54_RS01580 0.81 -1.5 342,021 + LRK54_RS01580 0.81 -1.2 342,021 + LRK54_RS01580 0.81 -2.2 342,021 + LRK54_RS01580 0.81 -2.5 342,021 + LRK54_RS01580 0.81 -1.6 342,021 + LRK54_RS01580 0.81 -3.3 342,021 + LRK54_RS01580 0.81 -0.8 342,021 + LRK54_RS01580 0.81 +2.0 342,021 + LRK54_RS01580 0.81 -2.2 342,021 + LRK54_RS01580 0.81 +0.0 342,021 + LRK54_RS01580 0.81 +0.7 342,021 + LRK54_RS01580 0.81 -1.2 342,021 + LRK54_RS01580 0.81 -1.0 342,021 + LRK54_RS01580 0.81 -0.6 342,022 - LRK54_RS01580 0.81 -1.3 342,022 - LRK54_RS01580 0.81 -1.6 342,022 - LRK54_RS01580 0.81 -1.5 342,022 - LRK54_RS01580 0.81 -2.2 342,022 - LRK54_RS01580 0.81 -1.2 342,329 + LRK54_RS01585 0.23 -1.0 342,410 + LRK54_RS01585 0.34 -0.7 342,410 + LRK54_RS01585 0.34 -2.5 342,411 - LRK54_RS01585 0.34 -1.8 342,411 - LRK54_RS01585 0.34 -0.6 342,530 + LRK54_RS01585 0.50 -1.7 342,530 + LRK54_RS01585 0.50 +1.4 342,531 - LRK54_RS01585 0.50 -1.3 342,640 + LRK54_RS01585 0.65 -1.6 342,640 + LRK54_RS01585 0.65 -1.0 342,640 + LRK54_RS01585 0.65 -0.0 342,770 + LRK54_RS01585 0.83 +0.6 342,771 - LRK54_RS01585 0.83 +1.2 342,803 + LRK54_RS01585 0.88 -0.9 342,803 + LRK54_RS01585 0.88 +0.1 342,803 + LRK54_RS01585 0.88 -1.9 342,803 + LRK54_RS01585 0.88 +0.1 342,803 + LRK54_RS01585 0.88 -0.9 342,803 + LRK54_RS01585 0.88 -2.6 342,803 + LRK54_RS01585 0.88 -0.4 342,803 + LRK54_RS01585 0.88 -1.3 342,803 + LRK54_RS01585 0.88 -1.7 342,803 + LRK54_RS01585 0.88 -0.5 342,803 + LRK54_RS01585 0.88 -1.9 342,803 + LRK54_RS01585 0.88 -1.0 342,803 + LRK54_RS01585 0.88 -2.3 342,803 + LRK54_RS01585 0.88 +0.8 342,803 + LRK54_RS01585 0.88 +1.0 342,803 + LRK54_RS01585 0.88 -2.2 342,804 - LRK54_RS01585 0.88 -0.4 342,804 - LRK54_RS01585 0.88 -2.3 342,804 - LRK54_RS01585 0.88 +0.3 342,804 - LRK54_RS01585 0.88 +0.1 342,804 - LRK54_RS01585 0.88 +0.1 342,804 - LRK54_RS01585 0.88 -1.7 342,804 - LRK54_RS01585 0.88 -3.0 342,804 - LRK54_RS01585 0.88 -1.7 342,804 - LRK54_RS01585 0.88 -0.7 342,804 - LRK54_RS01585 0.88 -0.4 342,822 - -0.9 342,891 + +0.4 342,891 + -0.7 342,891 + +1.1 342,891 + +0.3 342,891 + -0.2 342,891 + -1.3 342,891 + +2.0 342,891 + -1.5 342,891 + -0.8 342,891 + -1.1 342,891 + -0.9 342,891 + -0.6 342,892 - -0.4 342,892 - -0.6 342,892 - +0.3 342,892 - -0.1 342,892 - +0.6 342,892 - +0.1 342,892 - -0.8 342,892 - -1.7 342,892 - -0.2 342,892 - -1.0 342,892 - -1.0 343,003 + LRK54_RS01590 0.19 -3.3 343,003 + LRK54_RS01590 0.19 +1.2 343,003 + LRK54_RS01590 0.19 -0.8 343,003 + LRK54_RS01590 0.19 -0.5 343,003 + LRK54_RS01590 0.19 +1.4 343,004 - LRK54_RS01590 0.20 -0.3 343,045 + LRK54_RS01590 0.27 -0.7 343,045 + LRK54_RS01590 0.27 -1.7 343,045 + LRK54_RS01590 0.27 -2.9 343,045 + LRK54_RS01590 0.27 +0.8 343,045 + LRK54_RS01590 0.27 +0.3 343,045 + LRK54_RS01590 0.27 -2.1 343,045 + LRK54_RS01590 0.27 -0.4 343,045 + LRK54_RS01590 0.27 -0.3 343,045 + LRK54_RS01590 0.27 -0.7 343,045 + LRK54_RS01590 0.27 -0.6 343,045 + LRK54_RS01590 0.27 -0.9 343,045 + LRK54_RS01590 0.27 -0.6 343,045 + LRK54_RS01590 0.27 -1.3 343,045 + LRK54_RS01590 0.27 -1.5 343,046 - LRK54_RS01590 0.27 -0.4 343,046 - LRK54_RS01590 0.27 -0.6 343,046 - LRK54_RS01590 0.27 -2.3 343,046 - LRK54_RS01590 0.27 -0.4 343,046 - LRK54_RS01590 0.27 -1.3 343,046 - LRK54_RS01590 0.27 -2.1 343,046 - LRK54_RS01590 0.27 -0.9 343,046 - LRK54_RS01590 0.27 -1.5 343,046 - LRK54_RS01590 0.27 -0.3 343,046 - LRK54_RS01590 0.27 -2.8 343,046 - LRK54_RS01590 0.27 -1.6 343,046 - LRK54_RS01590 0.27 -1.1 343,046 - LRK54_RS01590 0.27 -2.6 343,046 - LRK54_RS01590 0.27 -2.0 343,046 - LRK54_RS01590 0.27 -1.2 343,046 - LRK54_RS01590 0.27 -3.0 343,046 - LRK54_RS01590 0.27 +1.1 343,046 - LRK54_RS01590 0.27 -1.4 343,046 - LRK54_RS01590 0.27 +0.5 343,046 - LRK54_RS01590 0.27 +0.1 343,046 - LRK54_RS01590 0.27 -1.3 343,046 - LRK54_RS01590 0.27 -0.4 343,046 - LRK54_RS01590 0.27 -2.1 343,060 + LRK54_RS01590 0.29 -0.7 343,060 + LRK54_RS01590 0.29 -1.7 343,060 + LRK54_RS01590 0.29 -0.5 343,060 + LRK54_RS01590 0.29 +2.1 343,060 + LRK54_RS01590 0.29 -0.7 343,061 - LRK54_RS01590 0.29 -0.2 343,061 - LRK54_RS01590 0.29 -1.4 343,061 - LRK54_RS01590 0.29 -3.7 343,061 - LRK54_RS01590 0.29 -1.6 343,061 - LRK54_RS01590 0.29 -1.4
Or see this region's nucleotide sequence