Experiment: R2A with Chromium(III) Chloride Hexahydrate 0.078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS01260 and LRK54_RS01270 are separated by 225 nucleotides LRK54_RS01270 and LRK54_RS01275 are separated by 81 nucleotides LRK54_RS01275 and LRK54_RS01280 are separated by 97 nucleotides LRK54_RS01280 and LRK54_RS01285 overlap by 4 nucleotides LRK54_RS01285 and LRK54_RS01290 are separated by 34 nucleotides
LRK54_RS01260: LRK54_RS01260 - DNA polymerase III subunit gamma/tau, at 276,260 to 277,945
_RS01260
LRK54_RS01270: LRK54_RS01270 - tRNA-Ser, at 278,171 to 278,263
_RS01270
LRK54_RS01275: LRK54_RS01275 - tRNA-Ser, at 278,345 to 278,456
_RS01275
LRK54_RS01280: LRK54_RS01280 - MBL fold metallo-hydrolase, at 278,554 to 279,312
_RS01280
LRK54_RS01285: LRK54_RS01285 - 3-deoxy-D-manno-octulosonic acid kinase, at 279,309 to 280,040
_RS01285
LRK54_RS01290: LRK54_RS01290 - glycosyltransferase family 9 protein, at 280,075 to 281,118
_RS01290
Position (kb) 278
279
280 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 277.939 kb on - strand at 277.939 kb on - strand at 277.993 kb on + strand at 277.993 kb on + strand at 277.993 kb on + strand at 277.993 kb on + strand at 277.993 kb on + strand at 277.993 kb on + strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 278.001 kb on + strand at 278.001 kb on + strand at 278.002 kb on - strand at 278.275 kb on - strand at 278.406 kb on - strand, within LRK54_RS01275 at 278.406 kb on - strand, within LRK54_RS01275 at 278.406 kb on - strand, within LRK54_RS01275 at 278.413 kb on - strand, within LRK54_RS01275 at 278.413 kb on - strand, within LRK54_RS01275 at 278.413 kb on - strand, within LRK54_RS01275 at 278.413 kb on - strand, within LRK54_RS01275 at 278.413 kb on - strand, within LRK54_RS01275 at 278.413 kb on - strand, within LRK54_RS01275 at 278.413 kb on - strand, within LRK54_RS01275 at 278.413 kb on - strand, within LRK54_RS01275 at 278.413 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.494 kb on - strand at 278.494 kb on - strand at 278.494 kb on - strand at 278.494 kb on - strand at 278.530 kb on - strand at 278.530 kb on - strand at 278.530 kb on - strand at 278.530 kb on - strand at 278.666 kb on + strand, within LRK54_RS01280 at 278.672 kb on + strand, within LRK54_RS01280 at 278.672 kb on + strand, within LRK54_RS01280 at 278.673 kb on - strand, within LRK54_RS01280 at 278.673 kb on - strand, within LRK54_RS01280 at 278.673 kb on - strand, within LRK54_RS01280 at 278.706 kb on - strand, within LRK54_RS01280 at 278.878 kb on + strand, within LRK54_RS01280 at 278.878 kb on + strand, within LRK54_RS01280 at 278.879 kb on - strand, within LRK54_RS01280 at 279.179 kb on + strand, within LRK54_RS01280 at 279.299 kb on - strand at 279.323 kb on + strand at 279.323 kb on + strand at 279.323 kb on + strand at 279.323 kb on + strand at 279.324 kb on - strand at 279.324 kb on - strand at 279.324 kb on - strand at 279.337 kb on + strand at 279.338 kb on - strand at 279.338 kb on - strand at 279.490 kb on + strand, within LRK54_RS01285 at 279.490 kb on + strand, within LRK54_RS01285 at 279.490 kb on + strand, within LRK54_RS01285 at 279.491 kb on - strand, within LRK54_RS01285 at 279.491 kb on - strand, within LRK54_RS01285 at 279.491 kb on - strand, within LRK54_RS01285 at 279.674 kb on + strand, within LRK54_RS01285 at 279.674 kb on + strand, within LRK54_RS01285 at 279.676 kb on + strand, within LRK54_RS01285 at 279.676 kb on + strand, within LRK54_RS01285 at 279.676 kb on + strand, within LRK54_RS01285 at 279.676 kb on + strand, within LRK54_RS01285 at 279.676 kb on + strand, within LRK54_RS01285 at 279.676 kb on + strand, within LRK54_RS01285 at 279.676 kb on + strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.753 kb on + strand, within LRK54_RS01285 at 279.753 kb on + strand, within LRK54_RS01285 at 279.753 kb on + strand, within LRK54_RS01285 at 279.753 kb on + strand, within LRK54_RS01285 at 279.753 kb on + strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.802 kb on + strand, within LRK54_RS01285 at 279.845 kb on - strand, within LRK54_RS01285 at 279.940 kb on + strand, within LRK54_RS01285 at 279.940 kb on + strand, within LRK54_RS01285 at 279.941 kb on - strand, within LRK54_RS01285 at 279.978 kb on + strand at 279.979 kb on - strand at 279.979 kb on - strand at 280.063 kb on + strand at 280.063 kb on + strand at 280.064 kb on - strand at 280.064 kb on - strand at 280.064 kb on - strand at 280.064 kb on - strand at 280.064 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chromium(III) Chloride Hexahydrate 0.078125 mM remove 277,939 - +0.1 277,939 - +1.4 277,993 + +0.5 277,993 + +0.5 277,993 + -0.1 277,993 + +0.1 277,993 + -1.0 277,993 + +0.9 277,994 - -1.1 277,994 - -0.4 277,994 - +0.9 277,994 - +0.1 277,994 - +0.5 277,994 - +0.0 277,994 - -0.2 277,994 - +0.5 277,994 - +2.5 277,994 - -0.9 277,994 - +1.5 277,994 - -0.8 277,994 - -0.8 277,994 - -0.4 277,994 - +0.3 277,994 - -0.3 278,001 + -0.9 278,001 + -2.4 278,002 - +1.1 278,275 - -0.2 278,406 - LRK54_RS01275 0.54 -0.1 278,406 - LRK54_RS01275 0.54 -0.4 278,406 - LRK54_RS01275 0.54 +2.5 278,413 - LRK54_RS01275 0.61 +0.6 278,413 - LRK54_RS01275 0.61 +0.5 278,413 - LRK54_RS01275 0.61 +1.7 278,413 - LRK54_RS01275 0.61 -1.1 278,413 - LRK54_RS01275 0.61 +0.7 278,413 - LRK54_RS01275 0.61 -0.3 278,413 - LRK54_RS01275 0.61 +0.4 278,413 - LRK54_RS01275 0.61 -0.6 278,413 - LRK54_RS01275 0.61 +0.3 278,415 - LRK54_RS01275 0.62 +0.9 278,415 - LRK54_RS01275 0.62 +0.5 278,415 - LRK54_RS01275 0.62 +0.5 278,415 - LRK54_RS01275 0.62 +1.5 278,415 - LRK54_RS01275 0.62 -0.2 278,415 - LRK54_RS01275 0.62 +0.3 278,415 - LRK54_RS01275 0.62 +0.5 278,415 - LRK54_RS01275 0.62 +0.1 278,415 - LRK54_RS01275 0.62 +2.0 278,415 - LRK54_RS01275 0.62 +0.4 278,415 - LRK54_RS01275 0.62 -0.0 278,415 - LRK54_RS01275 0.62 +0.5 278,415 - LRK54_RS01275 0.62 -0.0 278,415 - LRK54_RS01275 0.62 -1.1 278,415 - LRK54_RS01275 0.62 +0.0 278,415 - LRK54_RS01275 0.62 -0.2 278,415 - LRK54_RS01275 0.62 -1.3 278,415 - LRK54_RS01275 0.62 -1.0 278,415 - LRK54_RS01275 0.62 +0.6 278,415 - LRK54_RS01275 0.62 -0.1 278,415 - LRK54_RS01275 0.62 +1.3 278,415 - LRK54_RS01275 0.62 -1.1 278,415 - LRK54_RS01275 0.62 +0.7 278,415 - LRK54_RS01275 0.62 +1.0 278,415 - LRK54_RS01275 0.62 +0.5 278,415 - LRK54_RS01275 0.62 +0.5 278,494 - +0.1 278,494 - +0.8 278,494 - -0.5 278,494 - +1.2 278,530 - +0.1 278,530 - -0.2 278,530 - -1.1 278,530 - +2.5 278,666 + LRK54_RS01280 0.15 -0.1 278,672 + LRK54_RS01280 0.16 +0.5 278,672 + LRK54_RS01280 0.16 -0.4 278,673 - LRK54_RS01280 0.16 +1.5 278,673 - LRK54_RS01280 0.16 -0.4 278,673 - LRK54_RS01280 0.16 -0.4 278,706 - LRK54_RS01280 0.20 -0.5 278,878 + LRK54_RS01280 0.43 -0.1 278,878 + LRK54_RS01280 0.43 -1.0 278,879 - LRK54_RS01280 0.43 -0.7 279,179 + LRK54_RS01280 0.82 +1.5 279,299 - +3.5 279,323 + -1.7 279,323 + -1.7 279,323 + -0.5 279,323 + -0.1 279,324 - +1.9 279,324 - +1.7 279,324 - -0.4 279,337 + -0.1 279,338 - +0.5 279,338 - -0.9 279,490 + LRK54_RS01285 0.25 -0.9 279,490 + LRK54_RS01285 0.25 +0.5 279,490 + LRK54_RS01285 0.25 -1.0 279,491 - LRK54_RS01285 0.25 -0.5 279,491 - LRK54_RS01285 0.25 +0.9 279,491 - LRK54_RS01285 0.25 -0.2 279,674 + LRK54_RS01285 0.50 -0.7 279,674 + LRK54_RS01285 0.50 -1.5 279,676 + LRK54_RS01285 0.50 -1.0 279,676 + LRK54_RS01285 0.50 +0.1 279,676 + LRK54_RS01285 0.50 -0.1 279,676 + LRK54_RS01285 0.50 -0.1 279,676 + LRK54_RS01285 0.50 -0.5 279,676 + LRK54_RS01285 0.50 -0.9 279,676 + LRK54_RS01285 0.50 +0.5 279,677 - LRK54_RS01285 0.50 +0.9 279,677 - LRK54_RS01285 0.50 -2.4 279,677 - LRK54_RS01285 0.50 -1.2 279,677 - LRK54_RS01285 0.50 -0.5 279,677 - LRK54_RS01285 0.50 -0.5 279,677 - LRK54_RS01285 0.50 +0.1 279,677 - LRK54_RS01285 0.50 +0.8 279,677 - LRK54_RS01285 0.50 -1.5 279,677 - LRK54_RS01285 0.50 -0.5 279,677 - LRK54_RS01285 0.50 +0.1 279,677 - LRK54_RS01285 0.50 +0.9 279,677 - LRK54_RS01285 0.50 -0.1 279,677 - LRK54_RS01285 0.50 -0.7 279,677 - LRK54_RS01285 0.50 -0.5 279,677 - LRK54_RS01285 0.50 -0.7 279,753 + LRK54_RS01285 0.61 +0.1 279,753 + LRK54_RS01285 0.61 +0.9 279,753 + LRK54_RS01285 0.61 -0.1 279,753 + LRK54_RS01285 0.61 -2.2 279,753 + LRK54_RS01285 0.61 +0.3 279,754 - LRK54_RS01285 0.61 +1.5 279,754 - LRK54_RS01285 0.61 -2.1 279,754 - LRK54_RS01285 0.61 -1.1 279,754 - LRK54_RS01285 0.61 -1.9 279,754 - LRK54_RS01285 0.61 +0.0 279,754 - LRK54_RS01285 0.61 +0.9 279,754 - LRK54_RS01285 0.61 -1.1 279,754 - LRK54_RS01285 0.61 -0.9 279,754 - LRK54_RS01285 0.61 -0.7 279,754 - LRK54_RS01285 0.61 +2.5 279,754 - LRK54_RS01285 0.61 -0.7 279,802 + LRK54_RS01285 0.67 +1.2 279,845 - LRK54_RS01285 0.73 +0.3 279,940 + LRK54_RS01285 0.86 -0.9 279,940 + LRK54_RS01285 0.86 -2.0 279,941 - LRK54_RS01285 0.86 +0.5 279,978 + -1.6 279,979 - -0.4 279,979 - -0.5 280,063 + +0.1 280,063 + +0.7 280,064 - -0.5 280,064 - +0.8 280,064 - -0.4 280,064 - -1.2 280,064 - +0.7
Or see this region's nucleotide sequence