Experiment: A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS05100 and HSERO_RS05105 are separated by 27 nucleotides HSERO_RS05105 and HSERO_RS05110 are separated by 2 nucleotides HSERO_RS05110 and HSERO_RS05115 are separated by 200 nucleotides
HSERO_RS05100: HSERO_RS05100 - sugar ABC transporter permease, at 1,127,262 to 1,128,275
_RS05100
HSERO_RS05105: HSERO_RS05105 - ribokinase, at 1,128,303 to 1,129,262
_RS05105
HSERO_RS05110: HSERO_RS05110 - RbsD or FucU transport protein, at 1,129,265 to 1,129,717
_RS05110
HSERO_RS05115: HSERO_RS05115 - aldehyde dehydrogenase, at 1,129,918 to 1,132,299
_RS05115
Position (kb)
1129
1130 Strain fitness (log2 ratio)
-1
0
1 at 1128.379 kb on + strand at 1128.477 kb on + strand, within HSERO_RS05105 at 1128.726 kb on - strand, within HSERO_RS05105 at 1128.764 kb on - strand, within HSERO_RS05105 at 1128.843 kb on + strand, within HSERO_RS05105 at 1129.263 kb on - strand at 1129.314 kb on + strand, within HSERO_RS05110 at 1129.671 kb on + strand, within HSERO_RS05110 at 1129.970 kb on - strand at 1130.025 kb on + strand at 1130.385 kb on - strand, within HSERO_RS05115 at 1130.557 kb on - strand, within HSERO_RS05115 at 1130.701 kb on - strand, within HSERO_RS05115
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.04 mg/ml remove 1,128,379 + +0.0 1,128,477 + HSERO_RS05105 0.18 +0.0 1,128,726 - HSERO_RS05105 0.44 +0.6 1,128,764 - HSERO_RS05105 0.48 -0.6 1,128,843 + HSERO_RS05105 0.56 -0.0 1,129,263 - -0.0 1,129,314 + HSERO_RS05110 0.11 -0.5 1,129,671 + HSERO_RS05110 0.90 -0.1 1,129,970 - +0.4 1,130,025 + -0.0 1,130,385 - HSERO_RS05115 0.20 +0.9 1,130,557 - HSERO_RS05115 0.27 +0.7 1,130,701 - HSERO_RS05115 0.33 -0.4
Or see this region's nucleotide sequence