Experiment: A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS00255 and HSERO_RS00260 overlap by 4 nucleotides HSERO_RS00260 and HSERO_RS00265 are separated by 19 nucleotides HSERO_RS00265 and HSERO_RS00270 are separated by 345 nucleotides HSERO_RS00270 and HSERO_RS00275 are separated by 126 nucleotides
HSERO_RS00255: HSERO_RS00255 - iron transporter FeoB, at 64,343 to 66,169
_RS00255
HSERO_RS00260: HSERO_RS00260 - hypothetical protein, at 66,166 to 66,453
_RS00260
HSERO_RS00265: HSERO_RS00265 - threonine dehydratase, at 66,473 to 67,678
_RS00265
HSERO_RS00270: HSERO_RS00270 - hypothetical protein, at 68,024 to 68,380
_RS00270
HSERO_RS00275: HSERO_RS00275 - methylated-DNA--protein-cysteine methyltransferase, at 68,507 to 69,079
_RS00275
Position (kb) 66
67
68 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 65.481 kb on + strand, within HSERO_RS00255 at 65.726 kb on + strand, within HSERO_RS00255 at 65.726 kb on + strand, within HSERO_RS00255 at 65.778 kb on - strand, within HSERO_RS00255 at 65.812 kb on - strand, within HSERO_RS00255 at 65.820 kb on + strand, within HSERO_RS00255 at 65.925 kb on - strand, within HSERO_RS00255 at 65.954 kb on + strand, within HSERO_RS00255 at 65.962 kb on - strand, within HSERO_RS00255 at 66.118 kb on + strand at 66.118 kb on + strand at 66.148 kb on - strand at 66.149 kb on - strand at 66.363 kb on - strand, within HSERO_RS00260 at 66.709 kb on + strand, within HSERO_RS00265 at 66.795 kb on - strand, within HSERO_RS00265 at 66.969 kb on - strand, within HSERO_RS00265 at 67.049 kb on - strand, within HSERO_RS00265 at 67.180 kb on - strand, within HSERO_RS00265 at 67.525 kb on + strand, within HSERO_RS00265 at 67.541 kb on + strand, within HSERO_RS00265 at 67.584 kb on + strand at 67.584 kb on + strand at 67.584 kb on + strand at 67.643 kb on + strand at 67.833 kb on - strand at 67.878 kb on - strand at 67.969 kb on + strand at 68.034 kb on + strand at 68.146 kb on + strand, within HSERO_RS00270 at 68.229 kb on - strand, within HSERO_RS00270 at 68.533 kb on - strand at 68.632 kb on + strand, within HSERO_RS00275 at 68.647 kb on - strand, within HSERO_RS00275
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.04 mg/ml remove 65,481 + HSERO_RS00255 0.62 -0.0 65,726 + HSERO_RS00255 0.76 -0.4 65,726 + HSERO_RS00255 0.76 +0.0 65,778 - HSERO_RS00255 0.79 +0.1 65,812 - HSERO_RS00255 0.80 -0.1 65,820 + HSERO_RS00255 0.81 +0.3 65,925 - HSERO_RS00255 0.87 -0.6 65,954 + HSERO_RS00255 0.88 -1.4 65,962 - HSERO_RS00255 0.89 +0.2 66,118 + -0.3 66,118 + -1.3 66,148 - +0.6 66,149 - -0.1 66,363 - HSERO_RS00260 0.68 -0.1 66,709 + HSERO_RS00265 0.20 -1.0 66,795 - HSERO_RS00265 0.27 -1.1 66,969 - HSERO_RS00265 0.41 -2.3 67,049 - HSERO_RS00265 0.48 -0.4 67,180 - HSERO_RS00265 0.59 +0.2 67,525 + HSERO_RS00265 0.87 -1.0 67,541 + HSERO_RS00265 0.89 -1.0 67,584 + -0.8 67,584 + -3.3 67,584 + -0.3 67,643 + -0.4 67,833 - +0.9 67,878 - +0.5 67,969 + -0.4 68,034 + +0.2 68,146 + HSERO_RS00270 0.34 +0.2 68,229 - HSERO_RS00270 0.57 -1.3 68,533 - +0.0 68,632 + HSERO_RS00275 0.22 -0.9 68,647 - HSERO_RS00275 0.24 -0.8
Or see this region's nucleotide sequence