Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31990 and RR42_RS31995 are separated by 99 nucleotides RR42_RS31995 and RR42_RS32000 are separated by 58 nucleotides RR42_RS32000 and RR42_RS32005 are separated by 19 nucleotides
RR42_RS31990: RR42_RS31990 - delta 4, 5-alpha steroid dehydrogenase, at 2,554,517 to 2,556,223
_RS31990
RR42_RS31995: RR42_RS31995 - flavin reductase, at 2,556,323 to 2,556,841
_RS31995
RR42_RS32000: RR42_RS32000 - 3-oxosteroid 1-dehydrogenase, at 2,556,900 to 2,558,642
_RS32000
RR42_RS32005: RR42_RS32005 - flavin-dependent monooxygenase, at 2,558,662 to 2,559,855
_RS32005
Position (kb)
2556
2557
2558
2559 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2555.974 kb on - strand, within RR42_RS31990 at 2556.043 kb on - strand, within RR42_RS31990 at 2556.378 kb on + strand, within RR42_RS31995 at 2556.378 kb on + strand, within RR42_RS31995 at 2556.545 kb on - strand, within RR42_RS31995 at 2556.805 kb on + strand at 2556.806 kb on - strand at 2556.829 kb on + strand at 2556.830 kb on - strand at 2556.858 kb on - strand at 2556.858 kb on - strand at 2556.858 kb on - strand at 2556.858 kb on - strand at 2557.211 kb on - strand, within RR42_RS32000 at 2557.211 kb on - strand, within RR42_RS32000 at 2557.214 kb on + strand, within RR42_RS32000 at 2557.215 kb on - strand, within RR42_RS32000 at 2557.502 kb on - strand, within RR42_RS32000 at 2557.526 kb on - strand, within RR42_RS32000 at 2557.766 kb on - strand, within RR42_RS32000 at 2557.998 kb on - strand, within RR42_RS32000 at 2557.998 kb on - strand, within RR42_RS32000 at 2558.291 kb on - strand, within RR42_RS32000 at 2558.318 kb on - strand, within RR42_RS32000 at 2558.348 kb on - strand, within RR42_RS32000 at 2558.416 kb on + strand, within RR42_RS32000 at 2558.417 kb on - strand, within RR42_RS32000 at 2558.668 kb on - strand at 2558.741 kb on + strand at 2558.799 kb on - strand, within RR42_RS32005 at 2558.799 kb on - strand, within RR42_RS32005 at 2559.068 kb on + strand, within RR42_RS32005 at 2559.068 kb on + strand, within RR42_RS32005 at 2559.158 kb on + strand, within RR42_RS32005 at 2559.318 kb on - strand, within RR42_RS32005 at 2559.347 kb on + strand, within RR42_RS32005 at 2559.489 kb on + strand, within RR42_RS32005 at 2559.491 kb on + strand, within RR42_RS32005 at 2559.492 kb on - strand, within RR42_RS32005 at 2559.510 kb on + strand, within RR42_RS32005 at 2559.593 kb on + strand, within RR42_RS32005 at 2559.594 kb on - strand, within RR42_RS32005
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 2,555,974 - RR42_RS31990 0.85 -0.2 2,556,043 - RR42_RS31990 0.89 -1.0 2,556,378 + RR42_RS31995 0.11 -0.8 2,556,378 + RR42_RS31995 0.11 -0.0 2,556,545 - RR42_RS31995 0.43 +0.6 2,556,805 + +0.5 2,556,806 - -0.2 2,556,829 + +1.2 2,556,830 - +0.3 2,556,858 - +0.5 2,556,858 - +0.1 2,556,858 - +1.7 2,556,858 - +0.1 2,557,211 - RR42_RS32000 0.18 +0.3 2,557,211 - RR42_RS32000 0.18 +0.9 2,557,214 + RR42_RS32000 0.18 -0.0 2,557,215 - RR42_RS32000 0.18 +0.9 2,557,502 - RR42_RS32000 0.35 +0.5 2,557,526 - RR42_RS32000 0.36 -0.2 2,557,766 - RR42_RS32000 0.50 +0.2 2,557,998 - RR42_RS32000 0.63 -0.0 2,557,998 - RR42_RS32000 0.63 +0.4 2,558,291 - RR42_RS32000 0.80 +0.4 2,558,318 - RR42_RS32000 0.81 -1.0 2,558,348 - RR42_RS32000 0.83 +0.0 2,558,416 + RR42_RS32000 0.87 +1.2 2,558,417 - RR42_RS32000 0.87 +0.0 2,558,668 - +0.5 2,558,741 + -0.1 2,558,799 - RR42_RS32005 0.11 +0.3 2,558,799 - RR42_RS32005 0.11 +1.4 2,559,068 + RR42_RS32005 0.34 +2.8 2,559,068 + RR42_RS32005 0.34 -0.1 2,559,158 + RR42_RS32005 0.42 +0.3 2,559,318 - RR42_RS32005 0.55 +0.0 2,559,347 + RR42_RS32005 0.57 -0.7 2,559,489 + RR42_RS32005 0.69 -1.0 2,559,491 + RR42_RS32005 0.69 -0.2 2,559,492 - RR42_RS32005 0.70 +0.3 2,559,510 + RR42_RS32005 0.71 -0.2 2,559,593 + RR42_RS32005 0.78 -0.5 2,559,594 - RR42_RS32005 0.78 +0.7
Or see this region's nucleotide sequence