Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31055 and RR42_RS31060 are separated by 90 nucleotides RR42_RS31060 and RR42_RS31065 are separated by 250 nucleotides RR42_RS31065 and RR42_RS31070 are separated by 58 nucleotides
RR42_RS31055: RR42_RS31055 - 3-hydroxybutyryl-CoA dehydrogenase, at 2,347,160 to 2,348,086
_RS31055
RR42_RS31060: RR42_RS31060 - diguanylate cyclase, at 2,348,177 to 2,349,748
_RS31060
RR42_RS31065: RR42_RS31065 - GntR family transcriptional regulator, at 2,349,999 to 2,350,673
_RS31065
RR42_RS31070: RR42_RS31070 - dihydroxy-acid dehydratase, at 2,350,732 to 2,352,462
_RS31070
Position (kb)
2348
2349
2350 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2347.640 kb on + strand, within RR42_RS31055 at 2347.931 kb on - strand, within RR42_RS31055 at 2348.148 kb on + strand at 2348.253 kb on + strand at 2348.253 kb on + strand at 2348.253 kb on + strand at 2348.254 kb on - strand at 2348.254 kb on - strand at 2348.500 kb on + strand, within RR42_RS31060 at 2348.500 kb on + strand, within RR42_RS31060 at 2348.501 kb on - strand, within RR42_RS31060 at 2348.501 kb on - strand, within RR42_RS31060 at 2348.613 kb on + strand, within RR42_RS31060 at 2348.614 kb on - strand, within RR42_RS31060 at 2348.614 kb on - strand, within RR42_RS31060 at 2348.760 kb on + strand, within RR42_RS31060 at 2348.760 kb on + strand, within RR42_RS31060 at 2348.760 kb on + strand, within RR42_RS31060 at 2348.760 kb on + strand, within RR42_RS31060 at 2348.761 kb on - strand, within RR42_RS31060 at 2348.761 kb on - strand, within RR42_RS31060 at 2348.872 kb on + strand, within RR42_RS31060 at 2348.872 kb on + strand, within RR42_RS31060 at 2348.872 kb on + strand, within RR42_RS31060 at 2348.873 kb on - strand, within RR42_RS31060 at 2348.873 kb on - strand, within RR42_RS31060 at 2349.006 kb on + strand at 2349.041 kb on + strand, within RR42_RS31060 at 2349.041 kb on + strand, within RR42_RS31060 at 2349.071 kb on + strand, within RR42_RS31060 at 2349.149 kb on - strand, within RR42_RS31060 at 2349.168 kb on + strand, within RR42_RS31060 at 2349.168 kb on + strand, within RR42_RS31060 at 2349.169 kb on - strand, within RR42_RS31060 at 2349.169 kb on - strand, within RR42_RS31060 at 2349.293 kb on - strand, within RR42_RS31060 at 2349.298 kb on + strand, within RR42_RS31060 at 2349.323 kb on - strand, within RR42_RS31060 at 2349.323 kb on - strand, within RR42_RS31060 at 2349.425 kb on + strand, within RR42_RS31060 at 2349.520 kb on + strand, within RR42_RS31060 at 2349.520 kb on + strand, within RR42_RS31060 at 2349.521 kb on - strand, within RR42_RS31060 at 2349.537 kb on - strand, within RR42_RS31060 at 2349.537 kb on - strand, within RR42_RS31060 at 2349.537 kb on - strand, within RR42_RS31060 at 2349.553 kb on - strand, within RR42_RS31060 at 2349.553 kb on - strand, within RR42_RS31060 at 2349.615 kb on + strand at 2349.637 kb on + strand at 2349.637 kb on + strand at 2349.678 kb on + strand at 2349.740 kb on + strand at 2349.741 kb on - strand at 2349.741 kb on - strand at 2349.741 kb on - strand at 2349.775 kb on + strand at 2349.775 kb on + strand at 2349.988 kb on - strand at 2349.988 kb on - strand at 2349.997 kb on + strand at 2349.997 kb on + strand at 2349.998 kb on - strand at 2350.000 kb on + strand at 2350.000 kb on + strand at 2350.039 kb on + strand at 2350.039 kb on + strand at 2350.039 kb on + strand at 2350.040 kb on - strand at 2350.074 kb on + strand, within RR42_RS31065 at 2350.075 kb on - strand, within RR42_RS31065 at 2350.204 kb on + strand at 2350.421 kb on - strand, within RR42_RS31065 at 2350.661 kb on + strand at 2350.661 kb on + strand at 2350.676 kb on + strand at 2350.676 kb on + strand at 2350.676 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 2,347,640 + RR42_RS31055 0.52 -0.5 2,347,931 - RR42_RS31055 0.83 +0.2 2,348,148 + +0.1 2,348,253 + +1.3 2,348,253 + -0.2 2,348,253 + -0.5 2,348,254 - -0.7 2,348,254 - +0.4 2,348,500 + RR42_RS31060 0.21 +0.2 2,348,500 + RR42_RS31060 0.21 -0.3 2,348,501 - RR42_RS31060 0.21 +1.1 2,348,501 - RR42_RS31060 0.21 -0.3 2,348,613 + RR42_RS31060 0.28 -0.2 2,348,614 - RR42_RS31060 0.28 +1.6 2,348,614 - RR42_RS31060 0.28 -1.4 2,348,760 + RR42_RS31060 0.37 -0.3 2,348,760 + RR42_RS31060 0.37 -1.4 2,348,760 + RR42_RS31060 0.37 -0.1 2,348,760 + RR42_RS31060 0.37 +0.8 2,348,761 - RR42_RS31060 0.37 -1.0 2,348,761 - RR42_RS31060 0.37 -1.1 2,348,872 + RR42_RS31060 0.44 +0.4 2,348,872 + RR42_RS31060 0.44 -0.0 2,348,872 + RR42_RS31060 0.44 +0.3 2,348,873 - RR42_RS31060 0.44 -0.4 2,348,873 - RR42_RS31060 0.44 +0.6 2,349,006 + +2.5 2,349,041 + RR42_RS31060 0.55 -0.7 2,349,041 + RR42_RS31060 0.55 +0.5 2,349,071 + RR42_RS31060 0.57 +0.5 2,349,149 - RR42_RS31060 0.62 -0.0 2,349,168 + RR42_RS31060 0.63 -0.3 2,349,168 + RR42_RS31060 0.63 -0.2 2,349,169 - RR42_RS31060 0.63 -0.2 2,349,169 - RR42_RS31060 0.63 +1.4 2,349,293 - RR42_RS31060 0.71 -1.4 2,349,298 + RR42_RS31060 0.71 +0.4 2,349,323 - RR42_RS31060 0.73 +0.1 2,349,323 - RR42_RS31060 0.73 +1.8 2,349,425 + RR42_RS31060 0.79 -0.5 2,349,520 + RR42_RS31060 0.85 -0.2 2,349,520 + RR42_RS31060 0.85 -0.2 2,349,521 - RR42_RS31060 0.85 -1.0 2,349,537 - RR42_RS31060 0.87 -0.3 2,349,537 - RR42_RS31060 0.87 +1.2 2,349,537 - RR42_RS31060 0.87 -0.4 2,349,553 - RR42_RS31060 0.88 -0.3 2,349,553 - RR42_RS31060 0.88 -0.8 2,349,615 + +0.0 2,349,637 + -0.3 2,349,637 + +0.6 2,349,678 + -1.0 2,349,740 + -2.8 2,349,741 - +0.2 2,349,741 - +0.4 2,349,741 - -0.5 2,349,775 + -1.1 2,349,775 + +0.5 2,349,988 - -0.2 2,349,988 - +0.1 2,349,997 + -0.6 2,349,997 + +0.2 2,349,998 - -0.8 2,350,000 + +0.6 2,350,000 + -0.9 2,350,039 + +0.5 2,350,039 + -0.8 2,350,039 + +0.8 2,350,040 - +0.3 2,350,074 + RR42_RS31065 0.11 -1.0 2,350,075 - RR42_RS31065 0.11 +1.0 2,350,204 + -0.1 2,350,421 - RR42_RS31065 0.63 -0.6 2,350,661 + +0.2 2,350,661 + -0.5 2,350,676 + -0.8 2,350,676 + -1.5 2,350,676 + +0.7
Or see this region's nucleotide sequence