Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05095 and RR42_RS05100 are separated by 29 nucleotides RR42_RS05100 and RR42_RS05105 are separated by 2 nucleotides RR42_RS05105 and RR42_RS05110 are separated by 45 nucleotides
RR42_RS05095: RR42_RS05095 - 4-hyroxy-2-oxovalerate aldolase, at 1,124,735 to 1,125,739
_RS05095
RR42_RS05100: RR42_RS05100 - acetaldehyde dehydrogenase, at 1,125,769 to 1,126,734
_RS05100
RR42_RS05105: RR42_RS05105 - 2-keto-4-pentenoate hydratase, at 1,126,737 to 1,127,528
_RS05105
RR42_RS05110: RR42_RS05110 - betaine-aldehyde dehydrogenase, at 1,127,574 to 1,129,043
_RS05110
Position (kb)
1125
1126
1127 Strain fitness (log2 ratio)
-1
0
1
2 at 1124.883 kb on + strand, within RR42_RS05095 at 1125.349 kb on + strand, within RR42_RS05095 at 1125.570 kb on + strand, within RR42_RS05095 at 1125.570 kb on + strand, within RR42_RS05095 at 1125.571 kb on - strand, within RR42_RS05095 at 1125.675 kb on + strand at 1125.843 kb on + strand at 1125.878 kb on + strand, within RR42_RS05100 at 1126.344 kb on + strand, within RR42_RS05100 at 1126.345 kb on - strand, within RR42_RS05100 at 1126.345 kb on - strand, within RR42_RS05100 at 1126.397 kb on + strand, within RR42_RS05100 at 1126.398 kb on - strand, within RR42_RS05100 at 1127.608 kb on + strand at 1127.608 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 1,124,883 + RR42_RS05095 0.15 -0.3 1,125,349 + RR42_RS05095 0.61 -0.4 1,125,570 + RR42_RS05095 0.83 +1.8 1,125,570 + RR42_RS05095 0.83 -0.4 1,125,571 - RR42_RS05095 0.83 +2.0 1,125,675 + +2.4 1,125,843 + +0.1 1,125,878 + RR42_RS05100 0.11 -0.8 1,126,344 + RR42_RS05100 0.60 -0.0 1,126,345 - RR42_RS05100 0.60 +1.1 1,126,345 - RR42_RS05100 0.60 +0.1 1,126,397 + RR42_RS05100 0.65 -0.0 1,126,398 - RR42_RS05100 0.65 -0.8 1,127,608 + +0.2 1,127,608 + +1.5
Or see this region's nucleotide sequence