Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05330 and MMJJ_RS05335 are separated by 67 nucleotides MMJJ_RS05335 and MMJJ_RS05340 are separated by 55 nucleotides MMJJ_RS05340 and MMJJ_RS05345 are separated by 179 nucleotides
MMJJ_RS05330: MMJJ_RS05330 - DNA polymerase II large subunit, at 988,990 to 992,385
_RS05330
MMJJ_RS05335: MMJJ_RS05335 - heavy metal-binding domain-containing protein, at 992,453 to 992,725
_RS05335
MMJJ_RS05340: MMJJ_RS05340 - AAA family ATPase, at 992,781 to 993,896
_RS05340
MMJJ_RS05345: MMJJ_RS05345 - ATP-dependent Clp protease proteolytic subunit, at 994,076 to 994,900
_RS05345
Position (kb) 992
993
994 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 992.289 kb on - strand at 992.383 kb on - strand at 992.403 kb on - strand at 992.403 kb on - strand at 992.406 kb on - strand at 992.406 kb on - strand at 992.407 kb on + strand at 992.407 kb on + strand at 992.408 kb on - strand at 992.408 kb on - strand at 992.408 kb on - strand at 992.408 kb on - strand at 992.408 kb on - strand at 992.408 kb on - strand at 992.408 kb on - strand at 992.408 kb on - strand at 992.408 kb on - strand at 992.408 kb on - strand at 992.408 kb on - strand at 992.408 kb on - strand at 992.409 kb on + strand at 992.409 kb on + strand at 992.409 kb on + strand at 992.409 kb on + strand at 992.409 kb on + strand at 992.409 kb on + strand at 992.410 kb on - strand at 992.411 kb on + strand at 992.411 kb on + strand at 992.411 kb on + strand at 992.422 kb on + strand at 992.423 kb on - strand at 992.423 kb on - strand at 992.428 kb on + strand at 992.461 kb on + strand at 992.513 kb on + strand, within MMJJ_RS05335 at 992.526 kb on - strand, within MMJJ_RS05335 at 992.526 kb on - strand, within MMJJ_RS05335 at 992.623 kb on + strand, within MMJJ_RS05335 at 992.625 kb on - strand, within MMJJ_RS05335 at 992.625 kb on - strand, within MMJJ_RS05335 at 992.626 kb on + strand, within MMJJ_RS05335 at 992.626 kb on + strand, within MMJJ_RS05335 at 992.626 kb on + strand, within MMJJ_RS05335 at 992.651 kb on - strand, within MMJJ_RS05335 at 992.687 kb on - strand, within MMJJ_RS05335 at 992.687 kb on - strand, within MMJJ_RS05335 at 992.693 kb on - strand, within MMJJ_RS05335 at 992.693 kb on - strand, within MMJJ_RS05335 at 992.694 kb on + strand, within MMJJ_RS05335 at 992.727 kb on - strand at 992.727 kb on - strand at 992.728 kb on + strand at 992.728 kb on + strand at 992.730 kb on + strand at 992.730 kb on + strand at 992.731 kb on - strand at 992.731 kb on - strand at 992.731 kb on - strand at 992.731 kb on - strand at 992.731 kb on - strand at 992.732 kb on + strand at 992.788 kb on - strand at 992.789 kb on + strand at 992.790 kb on - strand at 992.790 kb on - strand at 992.796 kb on - strand at 992.950 kb on - strand, within MMJJ_RS05340 at 992.951 kb on + strand, within MMJJ_RS05340 at 992.951 kb on + strand, within MMJJ_RS05340 at 992.977 kb on + strand, within MMJJ_RS05340 at 992.996 kb on + strand, within MMJJ_RS05340 at 993.152 kb on - strand, within MMJJ_RS05340 at 993.165 kb on - strand, within MMJJ_RS05340 at 993.168 kb on - strand, within MMJJ_RS05340 at 993.177 kb on - strand, within MMJJ_RS05340 at 993.177 kb on - strand, within MMJJ_RS05340 at 993.178 kb on + strand, within MMJJ_RS05340 at 993.178 kb on + strand, within MMJJ_RS05340 at 993.236 kb on - strand, within MMJJ_RS05340 at 993.240 kb on - strand, within MMJJ_RS05340 at 993.240 kb on - strand, within MMJJ_RS05340 at 993.255 kb on + strand, within MMJJ_RS05340 at 993.320 kb on - strand, within MMJJ_RS05340 at 993.339 kb on - strand, within MMJJ_RS05340 at 993.339 kb on - strand, within MMJJ_RS05340 at 993.340 kb on + strand, within MMJJ_RS05340 at 993.340 kb on + strand, within MMJJ_RS05340 at 993.352 kb on + strand, within MMJJ_RS05340 at 993.418 kb on + strand, within MMJJ_RS05340 at 993.418 kb on + strand, within MMJJ_RS05340 at 993.447 kb on - strand, within MMJJ_RS05340 at 993.466 kb on - strand, within MMJJ_RS05340 at 993.466 kb on - strand, within MMJJ_RS05340 at 993.471 kb on - strand, within MMJJ_RS05340 at 993.561 kb on + strand, within MMJJ_RS05340 at 993.579 kb on + strand, within MMJJ_RS05340 at 993.583 kb on + strand, within MMJJ_RS05340 at 993.635 kb on - strand, within MMJJ_RS05340 at 993.636 kb on + strand, within MMJJ_RS05340 at 993.636 kb on + strand, within MMJJ_RS05340 at 993.636 kb on + strand, within MMJJ_RS05340 at 993.670 kb on + strand, within MMJJ_RS05340 at 993.670 kb on + strand, within MMJJ_RS05340 at 993.673 kb on - strand, within MMJJ_RS05340 at 993.673 kb on - strand, within MMJJ_RS05340 at 993.674 kb on + strand, within MMJJ_RS05340 at 993.718 kb on - strand, within MMJJ_RS05340 at 993.725 kb on - strand, within MMJJ_RS05340 at 993.726 kb on + strand, within MMJJ_RS05340 at 993.729 kb on - strand, within MMJJ_RS05340 at 993.744 kb on - strand, within MMJJ_RS05340 at 993.744 kb on - strand, within MMJJ_RS05340 at 993.750 kb on + strand, within MMJJ_RS05340 at 993.809 kb on + strand at 993.809 kb on + strand at 993.888 kb on + strand at 993.913 kb on - strand at 993.913 kb on - strand at 993.914 kb on + strand at 993.914 kb on + strand at 993.914 kb on + strand at 993.916 kb on + strand at 993.922 kb on + strand at 993.936 kb on + strand at 993.954 kb on - strand at 993.958 kb on + strand at 993.962 kb on + strand at 993.962 kb on + strand at 993.971 kb on - strand at 993.971 kb on - strand at 993.971 kb on - strand at 993.971 kb on - strand at 993.971 kb on - strand at 993.971 kb on - strand at 993.971 kb on - strand at 993.972 kb on + strand at 993.972 kb on + strand at 993.972 kb on + strand at 993.975 kb on - strand at 993.975 kb on - strand at 993.975 kb on - strand at 993.975 kb on - strand at 993.975 kb on - strand at 993.975 kb on - strand at 993.976 kb on + strand at 993.976 kb on + strand at 993.976 kb on + strand at 993.980 kb on - strand at 993.985 kb on - strand at 993.989 kb on + strand at 993.999 kb on - strand at 993.999 kb on - strand at 994.000 kb on + strand at 994.000 kb on + strand at 994.000 kb on + strand at 994.001 kb on - strand at 994.001 kb on - strand at 994.001 kb on - strand at 994.001 kb on - strand at 994.001 kb on - strand at 994.001 kb on - strand at 994.002 kb on + strand at 994.002 kb on + strand at 994.002 kb on + strand at 994.002 kb on + strand at 994.002 kb on + strand at 994.003 kb on - strand at 994.003 kb on - strand at 994.003 kb on - strand at 994.003 kb on - strand at 994.003 kb on - strand at 994.003 kb on - strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.004 kb on + strand at 994.005 kb on - strand at 994.005 kb on - strand at 994.005 kb on - strand at 994.005 kb on - strand at 994.005 kb on - strand at 994.005 kb on - strand at 994.005 kb on - strand at 994.005 kb on - strand at 994.005 kb on - strand at 994.005 kb on - strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.006 kb on + strand at 994.011 kb on + strand at 994.011 kb on + strand at 994.015 kb on - strand at 994.015 kb on - strand at 994.015 kb on - strand at 994.015 kb on - strand at 994.016 kb on + strand at 994.020 kb on + strand at 994.020 kb on + strand at 994.028 kb on - strand at 994.029 kb on + strand at 994.029 kb on + strand at 994.141 kb on - strand at 994.142 kb on + strand at 994.145 kb on - strand at 994.146 kb on + strand at 994.147 kb on - strand at 994.147 kb on - strand at 994.147 kb on - strand at 994.147 kb on - strand at 994.147 kb on - strand at 994.148 kb on + strand at 994.148 kb on + strand at 994.148 kb on + strand at 994.148 kb on + strand at 994.148 kb on + strand at 994.156 kb on + strand at 994.174 kb on + strand, within MMJJ_RS05345 at 994.259 kb on - strand, within MMJJ_RS05345 at 994.261 kb on - strand, within MMJJ_RS05345 at 994.262 kb on + strand, within MMJJ_RS05345 at 994.262 kb on + strand, within MMJJ_RS05345 at 994.265 kb on - strand, within MMJJ_RS05345 at 994.265 kb on - strand, within MMJJ_RS05345 at 994.265 kb on - strand, within MMJJ_RS05345 at 994.265 kb on - strand, within MMJJ_RS05345 at 994.266 kb on + strand, within MMJJ_RS05345 at 994.266 kb on + strand, within MMJJ_RS05345 at 994.266 kb on + strand, within MMJJ_RS05345 at 994.266 kb on + strand, within MMJJ_RS05345 at 994.266 kb on + strand, within MMJJ_RS05345 at 994.266 kb on + strand, within MMJJ_RS05345 at 994.266 kb on + strand, within MMJJ_RS05345 at 994.266 kb on + strand, within MMJJ_RS05345 at 994.266 kb on - strand, within MMJJ_RS05345 at 994.267 kb on - strand, within MMJJ_RS05345 at 994.268 kb on + strand, within MMJJ_RS05345 at 994.270 kb on - strand, within MMJJ_RS05345 at 994.275 kb on - strand, within MMJJ_RS05345 at 994.306 kb on - strand, within MMJJ_RS05345 at 994.306 kb on - strand, within MMJJ_RS05345 at 994.339 kb on + strand, within MMJJ_RS05345 at 994.339 kb on + strand, within MMJJ_RS05345 at 994.339 kb on + strand, within MMJJ_RS05345 at 994.342 kb on + strand, within MMJJ_RS05345 at 994.366 kb on + strand, within MMJJ_RS05345 at 994.366 kb on + strand, within MMJJ_RS05345 at 994.424 kb on + strand, within MMJJ_RS05345 at 994.424 kb on + strand, within MMJJ_RS05345 at 994.461 kb on - strand, within MMJJ_RS05345 at 994.486 kb on + strand, within MMJJ_RS05345 at 994.632 kb on - strand, within MMJJ_RS05345 at 994.655 kb on - strand, within MMJJ_RS05345 at 994.656 kb on + strand, within MMJJ_RS05345 at 994.660 kb on - strand, within MMJJ_RS05345 at 994.660 kb on - strand, within MMJJ_RS05345 at 994.661 kb on + strand, within MMJJ_RS05345 at 994.786 kb on - strand, within MMJJ_RS05345 at 994.862 kb on - strand at 994.863 kb on + strand at 994.892 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE remove 992,289 - -2.0 992,383 - +1.6 992,403 - -0.1 992,403 - +0.2 992,406 - +0.9 992,406 - -0.1 992,407 + -0.3 992,407 + -0.1 992,408 - -0.5 992,408 - -0.4 992,408 - -0.1 992,408 - -0.3 992,408 - +0.7 992,408 - +1.0 992,408 - -0.2 992,408 - -0.6 992,408 - -0.1 992,408 - -0.1 992,408 - +0.5 992,408 - +0.3 992,409 + -0.8 992,409 + +0.1 992,409 + +0.2 992,409 + +0.5 992,409 + -0.6 992,409 + +0.0 992,410 - +0.2 992,411 + +0.0 992,411 + -0.6 992,411 + -0.8 992,422 + -0.1 992,423 - +1.1 992,423 - -0.8 992,428 + -0.2 992,461 + -0.5 992,513 + MMJJ_RS05335 0.22 -1.2 992,526 - MMJJ_RS05335 0.27 -0.2 992,526 - MMJJ_RS05335 0.27 -0.1 992,623 + MMJJ_RS05335 0.62 +0.0 992,625 - MMJJ_RS05335 0.63 +0.8 992,625 - MMJJ_RS05335 0.63 +0.6 992,626 + MMJJ_RS05335 0.63 +0.1 992,626 + MMJJ_RS05335 0.63 +0.6 992,626 + MMJJ_RS05335 0.63 +0.4 992,651 - MMJJ_RS05335 0.73 -0.3 992,687 - MMJJ_RS05335 0.86 -0.7 992,687 - MMJJ_RS05335 0.86 +1.0 992,693 - MMJJ_RS05335 0.88 +1.0 992,693 - MMJJ_RS05335 0.88 -0.1 992,694 + MMJJ_RS05335 0.88 -0.1 992,727 - -0.4 992,727 - +0.3 992,728 + -0.7 992,728 + +0.5 992,730 + +0.0 992,730 + +0.3 992,731 - +0.4 992,731 - +0.0 992,731 - +0.2 992,731 - -0.4 992,731 - +0.4 992,732 + +0.0 992,788 - +0.1 992,789 + -0.5 992,790 - -0.9 992,790 - -0.2 992,796 - -0.0 992,950 - MMJJ_RS05340 0.15 +0.0 992,951 + MMJJ_RS05340 0.15 -0.5 992,951 + MMJJ_RS05340 0.15 +0.6 992,977 + MMJJ_RS05340 0.18 -0.5 992,996 + MMJJ_RS05340 0.19 -0.4 993,152 - MMJJ_RS05340 0.33 -0.4 993,165 - MMJJ_RS05340 0.34 -0.3 993,168 - MMJJ_RS05340 0.35 -0.1 993,177 - MMJJ_RS05340 0.35 -0.1 993,177 - MMJJ_RS05340 0.35 +0.1 993,178 + MMJJ_RS05340 0.36 +0.5 993,178 + MMJJ_RS05340 0.36 +0.1 993,236 - MMJJ_RS05340 0.41 -1.1 993,240 - MMJJ_RS05340 0.41 +0.3 993,240 - MMJJ_RS05340 0.41 +1.1 993,255 + MMJJ_RS05340 0.42 +0.4 993,320 - MMJJ_RS05340 0.48 +1.0 993,339 - MMJJ_RS05340 0.50 -0.3 993,339 - MMJJ_RS05340 0.50 -0.0 993,340 + MMJJ_RS05340 0.50 -0.2 993,340 + MMJJ_RS05340 0.50 -0.5 993,352 + MMJJ_RS05340 0.51 +0.2 993,418 + MMJJ_RS05340 0.57 -0.4 993,418 + MMJJ_RS05340 0.57 +0.2 993,447 - MMJJ_RS05340 0.60 -0.9 993,466 - MMJJ_RS05340 0.61 -0.2 993,466 - MMJJ_RS05340 0.61 +0.1 993,471 - MMJJ_RS05340 0.62 +0.3 993,561 + MMJJ_RS05340 0.70 +0.2 993,579 + MMJJ_RS05340 0.72 +1.5 993,583 + MMJJ_RS05340 0.72 +0.1 993,635 - MMJJ_RS05340 0.77 +0.4 993,636 + MMJJ_RS05340 0.77 +0.2 993,636 + MMJJ_RS05340 0.77 +0.5 993,636 + MMJJ_RS05340 0.77 +0.0 993,670 + MMJJ_RS05340 0.80 +1.1 993,670 + MMJJ_RS05340 0.80 -0.5 993,673 - MMJJ_RS05340 0.80 -0.2 993,673 - MMJJ_RS05340 0.80 -0.0 993,674 + MMJJ_RS05340 0.80 +0.7 993,718 - MMJJ_RS05340 0.84 +0.7 993,725 - MMJJ_RS05340 0.85 -0.0 993,726 + MMJJ_RS05340 0.85 +1.3 993,729 - MMJJ_RS05340 0.85 +0.4 993,744 - MMJJ_RS05340 0.86 -1.3 993,744 - MMJJ_RS05340 0.86 +0.2 993,750 + MMJJ_RS05340 0.87 +0.1 993,809 + -0.0 993,809 + -1.3 993,888 + +1.0 993,913 - -0.1 993,913 - +0.0 993,914 + -0.7 993,914 + -0.3 993,914 + +0.1 993,916 + +1.4 993,922 + +0.4 993,936 + -0.5 993,954 - +0.8 993,958 + -1.1 993,962 + +0.0 993,962 + +1.4 993,971 - -0.8 993,971 - +0.4 993,971 - -0.2 993,971 - -0.6 993,971 - +0.1 993,971 - +0.3 993,971 - -0.2 993,972 + -0.3 993,972 + -0.6 993,972 + +0.2 993,975 - -0.2 993,975 - -1.3 993,975 - -0.3 993,975 - +0.3 993,975 - -0.9 993,975 - +0.2 993,976 + +0.2 993,976 + -0.1 993,976 + +0.0 993,980 - -0.3 993,985 - +0.0 993,989 + -0.2 993,999 - -0.6 993,999 - +0.3 994,000 + -0.5 994,000 + +1.4 994,000 + +0.3 994,001 - -1.0 994,001 - +0.5 994,001 - -2.8 994,001 - +0.3 994,001 - -0.1 994,001 - +1.3 994,002 + -0.0 994,002 + +0.2 994,002 + +0.0 994,002 + +0.5 994,002 + +0.8 994,003 - -0.7 994,003 - -0.5 994,003 - -0.4 994,003 - +0.1 994,003 - +0.3 994,003 - +0.3 994,004 + -0.3 994,004 + +0.3 994,004 + -0.1 994,004 + -0.7 994,004 + +0.4 994,004 + +0.3 994,004 + -0.2 994,004 + -0.5 994,004 + +1.0 994,004 + +0.5 994,004 + +0.6 994,005 - +0.1 994,005 - -0.3 994,005 - +0.3 994,005 - +0.1 994,005 - -0.3 994,005 - +0.2 994,005 - -0.8 994,005 - -1.4 994,005 - -0.4 994,005 - +1.8 994,006 + -0.0 994,006 + -1.3 994,006 + +1.3 994,006 + -0.8 994,006 + -0.4 994,006 + -0.5 994,006 + -0.2 994,006 + +0.3 994,006 + -1.6 994,006 + -0.1 994,006 + -0.0 994,011 + -1.0 994,011 + +0.5 994,015 - -1.0 994,015 - -0.1 994,015 - -0.3 994,015 - +0.7 994,016 + +0.0 994,020 + -0.1 994,020 + +1.1 994,028 - -0.3 994,029 + +0.3 994,029 + -0.1 994,141 - -0.2 994,142 + +0.2 994,145 - -1.6 994,146 + -0.6 994,147 - -0.8 994,147 - -0.1 994,147 - +0.0 994,147 - +0.8 994,147 - -0.2 994,148 + -0.6 994,148 + -0.3 994,148 + -0.6 994,148 + +0.6 994,148 + +0.3 994,156 + -0.2 994,174 + MMJJ_RS05345 0.12 +0.3 994,259 - MMJJ_RS05345 0.22 -0.1 994,261 - MMJJ_RS05345 0.22 -0.6 994,262 + MMJJ_RS05345 0.23 -1.0 994,262 + MMJJ_RS05345 0.23 +0.7 994,265 - MMJJ_RS05345 0.23 -0.5 994,265 - MMJJ_RS05345 0.23 +1.1 994,265 - MMJJ_RS05345 0.23 -0.5 994,265 - MMJJ_RS05345 0.23 -0.3 994,266 + MMJJ_RS05345 0.23 +0.3 994,266 + MMJJ_RS05345 0.23 +1.8 994,266 + MMJJ_RS05345 0.23 +0.2 994,266 + MMJJ_RS05345 0.23 -0.1 994,266 + MMJJ_RS05345 0.23 +0.1 994,266 + MMJJ_RS05345 0.23 +0.7 994,266 + MMJJ_RS05345 0.23 +0.0 994,266 + MMJJ_RS05345 0.23 +0.8 994,266 - MMJJ_RS05345 0.23 +1.2 994,267 - MMJJ_RS05345 0.23 -0.6 994,268 + MMJJ_RS05345 0.23 +0.3 994,270 - MMJJ_RS05345 0.24 +0.0 994,275 - MMJJ_RS05345 0.24 -1.3 994,306 - MMJJ_RS05345 0.28 -0.3 994,306 - MMJJ_RS05345 0.28 -0.0 994,339 + MMJJ_RS05345 0.32 -0.9 994,339 + MMJJ_RS05345 0.32 -1.2 994,339 + MMJJ_RS05345 0.32 +0.0 994,342 + MMJJ_RS05345 0.32 -0.4 994,366 + MMJJ_RS05345 0.35 -0.2 994,366 + MMJJ_RS05345 0.35 +0.1 994,424 + MMJJ_RS05345 0.42 -0.6 994,424 + MMJJ_RS05345 0.42 +0.1 994,461 - MMJJ_RS05345 0.47 +0.1 994,486 + MMJJ_RS05345 0.50 -0.3 994,632 - MMJJ_RS05345 0.67 -0.7 994,655 - MMJJ_RS05345 0.70 +0.6 994,656 + MMJJ_RS05345 0.70 +0.6 994,660 - MMJJ_RS05345 0.71 -0.5 994,660 - MMJJ_RS05345 0.71 -0.6 994,661 + MMJJ_RS05345 0.71 +0.2 994,786 - MMJJ_RS05345 0.86 +0.2 994,862 - -0.2 994,863 + -0.3 994,892 + -0.2
Or see this region's nucleotide sequence