Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00690 and MMJJ_RS00695 are separated by 49 nucleotides MMJJ_RS00695 and MMJJ_RS00700 are separated by 9 nucleotides MMJJ_RS00700 and MMJJ_RS00705 are separated by 42 nucleotides
MMJJ_RS00690: MMJJ_RS00690 - nitrogenase subunit alpha, at 135,362 to 136,795
_RS00690
MMJJ_RS00695: MMJJ_RS00695 - P-II family nitrogen regulator, at 136,845 to 137,210
_RS00695
MMJJ_RS00700: MMJJ_RS00700 - P-II family nitrogen regulator, at 137,220 to 137,537
_RS00700
MMJJ_RS00705: MMJJ_RS00705 - nitrogenase iron protein, at 137,580 to 138,407
_RS00705
Position (kb) 136
137
138 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 135.876 kb on - strand, within MMJJ_RS00690 at 135.880 kb on - strand, within MMJJ_RS00690 at 135.881 kb on + strand, within MMJJ_RS00690 at 135.884 kb on - strand, within MMJJ_RS00690 at 135.894 kb on + strand, within MMJJ_RS00690 at 135.930 kb on + strand, within MMJJ_RS00690 at 135.975 kb on + strand, within MMJJ_RS00690 at 135.978 kb on - strand, within MMJJ_RS00690 at 135.979 kb on + strand, within MMJJ_RS00690 at 135.979 kb on + strand, within MMJJ_RS00690 at 136.087 kb on - strand, within MMJJ_RS00690 at 136.116 kb on - strand, within MMJJ_RS00690 at 136.117 kb on + strand, within MMJJ_RS00690 at 136.132 kb on - strand, within MMJJ_RS00690 at 136.132 kb on - strand, within MMJJ_RS00690 at 136.132 kb on - strand, within MMJJ_RS00690 at 136.132 kb on - strand, within MMJJ_RS00690 at 136.133 kb on + strand, within MMJJ_RS00690 at 136.134 kb on - strand, within MMJJ_RS00690 at 136.134 kb on - strand, within MMJJ_RS00690 at 136.158 kb on - strand, within MMJJ_RS00690 at 136.205 kb on - strand, within MMJJ_RS00690 at 136.206 kb on + strand, within MMJJ_RS00690 at 136.206 kb on + strand, within MMJJ_RS00690 at 136.213 kb on + strand, within MMJJ_RS00690 at 136.274 kb on - strand, within MMJJ_RS00690 at 136.274 kb on - strand, within MMJJ_RS00690 at 136.309 kb on - strand, within MMJJ_RS00690 at 136.313 kb on - strand, within MMJJ_RS00690 at 136.313 kb on - strand, within MMJJ_RS00690 at 136.313 kb on - strand, within MMJJ_RS00690 at 136.314 kb on + strand, within MMJJ_RS00690 at 136.322 kb on - strand, within MMJJ_RS00690 at 136.322 kb on - strand, within MMJJ_RS00690 at 136.323 kb on + strand, within MMJJ_RS00690 at 136.348 kb on - strand, within MMJJ_RS00690 at 136.349 kb on + strand, within MMJJ_RS00690 at 136.349 kb on + strand, within MMJJ_RS00690 at 136.349 kb on + strand, within MMJJ_RS00690 at 136.366 kb on + strand, within MMJJ_RS00690 at 136.366 kb on + strand, within MMJJ_RS00690 at 136.366 kb on + strand, within MMJJ_RS00690 at 136.405 kb on - strand, within MMJJ_RS00690 at 136.405 kb on - strand, within MMJJ_RS00690 at 136.405 kb on - strand, within MMJJ_RS00690 at 136.405 kb on - strand, within MMJJ_RS00690 at 136.481 kb on - strand, within MMJJ_RS00690 at 136.506 kb on + strand, within MMJJ_RS00690 at 136.570 kb on - strand, within MMJJ_RS00690 at 136.570 kb on - strand, within MMJJ_RS00690 at 136.571 kb on + strand, within MMJJ_RS00690 at 136.571 kb on + strand, within MMJJ_RS00690 at 136.578 kb on + strand, within MMJJ_RS00690 at 136.589 kb on - strand, within MMJJ_RS00690 at 136.590 kb on + strand, within MMJJ_RS00690 at 136.592 kb on - strand, within MMJJ_RS00690 at 136.593 kb on + strand, within MMJJ_RS00690 at 136.593 kb on + strand, within MMJJ_RS00690 at 136.593 kb on + strand, within MMJJ_RS00690 at 136.601 kb on - strand, within MMJJ_RS00690 at 136.601 kb on - strand, within MMJJ_RS00690 at 136.602 kb on + strand, within MMJJ_RS00690 at 136.610 kb on - strand, within MMJJ_RS00690 at 136.625 kb on + strand, within MMJJ_RS00690 at 136.625 kb on + strand, within MMJJ_RS00690 at 136.625 kb on + strand, within MMJJ_RS00690 at 136.634 kb on - strand, within MMJJ_RS00690 at 136.635 kb on + strand, within MMJJ_RS00690 at 136.635 kb on + strand, within MMJJ_RS00690 at 136.666 kb on - strand at 136.688 kb on + strand at 136.688 kb on + strand at 136.690 kb on - strand at 136.690 kb on - strand at 136.691 kb on + strand at 136.691 kb on + strand at 136.691 kb on + strand at 136.737 kb on - strand at 136.738 kb on + strand at 136.738 kb on + strand at 136.738 kb on + strand at 136.738 kb on + strand at 136.762 kb on - strand at 136.772 kb on - strand at 136.772 kb on - strand at 136.773 kb on + strand at 136.773 kb on + strand at 136.773 kb on + strand at 136.819 kb on + strand at 136.838 kb on - strand at 136.839 kb on + strand at 136.878 kb on - strand at 136.889 kb on - strand, within MMJJ_RS00695 at 136.889 kb on - strand, within MMJJ_RS00695 at 136.890 kb on + strand, within MMJJ_RS00695 at 136.905 kb on - strand, within MMJJ_RS00695 at 136.906 kb on + strand, within MMJJ_RS00695 at 136.906 kb on + strand, within MMJJ_RS00695 at 136.940 kb on - strand, within MMJJ_RS00695 at 136.941 kb on + strand, within MMJJ_RS00695 at 136.945 kb on + strand, within MMJJ_RS00695 at 136.952 kb on - strand, within MMJJ_RS00695 at 136.952 kb on - strand, within MMJJ_RS00695 at 136.952 kb on - strand, within MMJJ_RS00695 at 136.952 kb on - strand, within MMJJ_RS00695 at 136.965 kb on - strand, within MMJJ_RS00695 at 136.965 kb on - strand, within MMJJ_RS00695 at 136.995 kb on - strand, within MMJJ_RS00695 at 136.995 kb on - strand, within MMJJ_RS00695 at 136.995 kb on - strand, within MMJJ_RS00695 at 136.996 kb on + strand, within MMJJ_RS00695 at 137.001 kb on - strand, within MMJJ_RS00695 at 137.020 kb on - strand, within MMJJ_RS00695 at 137.020 kb on - strand, within MMJJ_RS00695 at 137.021 kb on + strand, within MMJJ_RS00695 at 137.021 kb on + strand, within MMJJ_RS00695 at 137.021 kb on + strand, within MMJJ_RS00695 at 137.021 kb on + strand, within MMJJ_RS00695 at 137.021 kb on + strand, within MMJJ_RS00695 at 137.021 kb on + strand at 137.021 kb on + strand, within MMJJ_RS00695 at 137.021 kb on + strand, within MMJJ_RS00695 at 137.021 kb on + strand, within MMJJ_RS00695 at 137.043 kb on - strand, within MMJJ_RS00695 at 137.199 kb on - strand at 137.200 kb on + strand at 137.210 kb on - strand at 137.210 kb on - strand at 137.212 kb on - strand at 137.213 kb on + strand at 137.221 kb on - strand at 137.221 kb on - strand at 137.222 kb on + strand at 137.223 kb on - strand at 137.223 kb on - strand at 137.224 kb on + strand at 137.224 kb on + strand at 137.251 kb on - strand at 137.252 kb on + strand, within MMJJ_RS00700 at 137.252 kb on + strand, within MMJJ_RS00700 at 137.262 kb on - strand, within MMJJ_RS00700 at 137.263 kb on + strand, within MMJJ_RS00700 at 137.263 kb on + strand, within MMJJ_RS00700 at 137.263 kb on + strand, within MMJJ_RS00700 at 137.271 kb on - strand, within MMJJ_RS00700 at 137.271 kb on - strand, within MMJJ_RS00700 at 137.272 kb on + strand, within MMJJ_RS00700 at 137.272 kb on + strand, within MMJJ_RS00700 at 137.277 kb on - strand, within MMJJ_RS00700 at 137.305 kb on - strand, within MMJJ_RS00700 at 137.306 kb on + strand, within MMJJ_RS00700 at 137.306 kb on + strand, within MMJJ_RS00700 at 137.319 kb on + strand, within MMJJ_RS00700 at 137.322 kb on - strand, within MMJJ_RS00700 at 137.323 kb on + strand, within MMJJ_RS00700 at 137.331 kb on - strand, within MMJJ_RS00700 at 137.332 kb on + strand, within MMJJ_RS00700 at 137.460 kb on - strand, within MMJJ_RS00700 at 137.505 kb on + strand, within MMJJ_RS00700 at 137.517 kb on - strand at 137.564 kb on + strand at 137.567 kb on - strand at 137.568 kb on + strand at 137.582 kb on + strand at 137.586 kb on - strand at 137.587 kb on + strand at 137.616 kb on - strand at 137.646 kb on - strand at 137.690 kb on + strand, within MMJJ_RS00705 at 137.711 kb on - strand, within MMJJ_RS00705 at 137.711 kb on - strand, within MMJJ_RS00705 at 137.712 kb on + strand, within MMJJ_RS00705 at 137.712 kb on + strand, within MMJJ_RS00705 at 137.720 kb on + strand, within MMJJ_RS00705 at 137.720 kb on + strand, within MMJJ_RS00705 at 137.788 kb on + strand, within MMJJ_RS00705 at 137.820 kb on - strand, within MMJJ_RS00705 at 137.820 kb on - strand, within MMJJ_RS00705 at 137.821 kb on + strand, within MMJJ_RS00705 at 137.823 kb on - strand, within MMJJ_RS00705 at 137.823 kb on - strand, within MMJJ_RS00705 at 137.824 kb on + strand, within MMJJ_RS00705 at 137.842 kb on + strand, within MMJJ_RS00705 at 137.849 kb on - strand, within MMJJ_RS00705 at 137.878 kb on + strand, within MMJJ_RS00705 at 137.959 kb on + strand, within MMJJ_RS00705 at 138.015 kb on - strand, within MMJJ_RS00705 at 138.016 kb on + strand, within MMJJ_RS00705 at 138.016 kb on + strand, within MMJJ_RS00705 at 138.016 kb on + strand, within MMJJ_RS00705 at 138.016 kb on + strand, within MMJJ_RS00705 at 138.016 kb on + strand, within MMJJ_RS00705 at 138.150 kb on - strand, within MMJJ_RS00705 at 138.150 kb on - strand, within MMJJ_RS00705 at 138.159 kb on - strand, within MMJJ_RS00705 at 138.160 kb on + strand, within MMJJ_RS00705 at 138.198 kb on + strand, within MMJJ_RS00705
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE remove 135,876 - MMJJ_RS00690 0.36 -0.4 135,880 - MMJJ_RS00690 0.36 +0.5 135,881 + MMJJ_RS00690 0.36 +0.7 135,884 - MMJJ_RS00690 0.36 -0.8 135,894 + MMJJ_RS00690 0.37 +0.2 135,930 + MMJJ_RS00690 0.40 -0.7 135,975 + MMJJ_RS00690 0.43 -0.3 135,978 - MMJJ_RS00690 0.43 -0.5 135,979 + MMJJ_RS00690 0.43 -0.6 135,979 + MMJJ_RS00690 0.43 +0.2 136,087 - MMJJ_RS00690 0.51 -1.6 136,116 - MMJJ_RS00690 0.53 -0.2 136,117 + MMJJ_RS00690 0.53 -0.5 136,132 - MMJJ_RS00690 0.54 +1.7 136,132 - MMJJ_RS00690 0.54 +0.1 136,132 - MMJJ_RS00690 0.54 +0.5 136,132 - MMJJ_RS00690 0.54 -0.5 136,133 + MMJJ_RS00690 0.54 -0.5 136,134 - MMJJ_RS00690 0.54 +0.0 136,134 - MMJJ_RS00690 0.54 +0.2 136,158 - MMJJ_RS00690 0.56 -1.1 136,205 - MMJJ_RS00690 0.59 +1.8 136,206 + MMJJ_RS00690 0.59 -0.5 136,206 + MMJJ_RS00690 0.59 +0.3 136,213 + MMJJ_RS00690 0.59 -0.7 136,274 - MMJJ_RS00690 0.64 +0.4 136,274 - MMJJ_RS00690 0.64 +0.2 136,309 - MMJJ_RS00690 0.66 +0.1 136,313 - MMJJ_RS00690 0.66 -0.4 136,313 - MMJJ_RS00690 0.66 +0.3 136,313 - MMJJ_RS00690 0.66 -0.1 136,314 + MMJJ_RS00690 0.66 -0.2 136,322 - MMJJ_RS00690 0.67 -0.5 136,322 - MMJJ_RS00690 0.67 +0.7 136,323 + MMJJ_RS00690 0.67 +0.2 136,348 - MMJJ_RS00690 0.69 -0.0 136,349 + MMJJ_RS00690 0.69 +0.3 136,349 + MMJJ_RS00690 0.69 -0.1 136,349 + MMJJ_RS00690 0.69 +0.7 136,366 + MMJJ_RS00690 0.70 -0.1 136,366 + MMJJ_RS00690 0.70 -0.1 136,366 + MMJJ_RS00690 0.70 +0.7 136,405 - MMJJ_RS00690 0.73 -0.6 136,405 - MMJJ_RS00690 0.73 -0.0 136,405 - MMJJ_RS00690 0.73 -0.5 136,405 - MMJJ_RS00690 0.73 +0.3 136,481 - MMJJ_RS00690 0.78 -0.4 136,506 + MMJJ_RS00690 0.80 +0.2 136,570 - MMJJ_RS00690 0.84 +0.5 136,570 - MMJJ_RS00690 0.84 +0.0 136,571 + MMJJ_RS00690 0.84 +0.5 136,571 + MMJJ_RS00690 0.84 +0.6 136,578 + MMJJ_RS00690 0.85 -0.2 136,589 - MMJJ_RS00690 0.86 +1.4 136,590 + MMJJ_RS00690 0.86 -0.6 136,592 - MMJJ_RS00690 0.86 +0.9 136,593 + MMJJ_RS00690 0.86 -0.1 136,593 + MMJJ_RS00690 0.86 +0.3 136,593 + MMJJ_RS00690 0.86 -0.1 136,601 - MMJJ_RS00690 0.86 -0.0 136,601 - MMJJ_RS00690 0.86 +0.1 136,602 + MMJJ_RS00690 0.86 -0.4 136,610 - MMJJ_RS00690 0.87 +0.0 136,625 + MMJJ_RS00690 0.88 -0.3 136,625 + MMJJ_RS00690 0.88 +0.4 136,625 + MMJJ_RS00690 0.88 -1.8 136,634 - MMJJ_RS00690 0.89 -0.3 136,635 + MMJJ_RS00690 0.89 +0.0 136,635 + MMJJ_RS00690 0.89 -1.4 136,666 - -1.2 136,688 + -0.5 136,688 + +0.3 136,690 - +0.1 136,690 - -0.4 136,691 + -0.1 136,691 + -2.2 136,691 + +0.2 136,737 - -0.7 136,738 + +0.1 136,738 + +1.1 136,738 + +0.3 136,738 + -0.3 136,762 - -0.4 136,772 - +0.2 136,772 - -0.2 136,773 + +0.0 136,773 + +0.3 136,773 + -0.0 136,819 + -0.3 136,838 - +0.1 136,839 + -0.3 136,878 - -0.6 136,889 - MMJJ_RS00695 0.12 -0.4 136,889 - MMJJ_RS00695 0.12 -0.1 136,890 + MMJJ_RS00695 0.12 -0.5 136,905 - MMJJ_RS00695 0.16 +0.4 136,906 + MMJJ_RS00695 0.17 +0.5 136,906 + MMJJ_RS00695 0.17 -0.3 136,940 - MMJJ_RS00695 0.26 +1.0 136,941 + MMJJ_RS00695 0.26 -0.0 136,945 + MMJJ_RS00695 0.27 +0.6 136,952 - MMJJ_RS00695 0.29 -0.3 136,952 - MMJJ_RS00695 0.29 -0.5 136,952 - MMJJ_RS00695 0.29 -0.7 136,952 - MMJJ_RS00695 0.29 +0.1 136,965 - MMJJ_RS00695 0.33 -0.1 136,965 - MMJJ_RS00695 0.33 +0.2 136,995 - MMJJ_RS00695 0.41 +0.5 136,995 - MMJJ_RS00695 0.41 +0.3 136,995 - MMJJ_RS00695 0.41 +0.1 136,996 + MMJJ_RS00695 0.41 +0.1 137,001 - MMJJ_RS00695 0.43 +0.3 137,020 - MMJJ_RS00695 0.48 +0.3 137,020 - MMJJ_RS00695 0.48 +0.1 137,021 + MMJJ_RS00695 0.48 +0.2 137,021 + MMJJ_RS00695 0.48 -0.2 137,021 + MMJJ_RS00695 0.48 +0.8 137,021 + MMJJ_RS00695 0.48 -0.0 137,021 + MMJJ_RS00695 0.48 +0.4 137,021 + +0.9 137,021 + MMJJ_RS00695 0.48 +2.5 137,021 + MMJJ_RS00695 0.48 +0.8 137,021 + MMJJ_RS00695 0.48 +0.0 137,043 - MMJJ_RS00695 0.54 -0.0 137,199 - -0.4 137,200 + -0.7 137,210 - -0.4 137,210 - +1.1 137,212 - +0.1 137,213 + -2.0 137,221 - -0.4 137,221 - -0.5 137,222 + -0.6 137,223 - -1.2 137,223 - -0.6 137,224 + -0.4 137,224 + -0.5 137,251 - +0.1 137,252 + MMJJ_RS00700 0.10 +0.4 137,252 + MMJJ_RS00700 0.10 -0.3 137,262 - MMJJ_RS00700 0.13 +0.2 137,263 + MMJJ_RS00700 0.14 +0.0 137,263 + MMJJ_RS00700 0.14 -0.5 137,263 + MMJJ_RS00700 0.14 +0.3 137,271 - MMJJ_RS00700 0.16 -0.5 137,271 - MMJJ_RS00700 0.16 -0.7 137,272 + MMJJ_RS00700 0.16 -0.1 137,272 + MMJJ_RS00700 0.16 -3.4 137,277 - MMJJ_RS00700 0.18 -1.5 137,305 - MMJJ_RS00700 0.27 +1.3 137,306 + MMJJ_RS00700 0.27 +0.3 137,306 + MMJJ_RS00700 0.27 -0.3 137,319 + MMJJ_RS00700 0.31 +0.1 137,322 - MMJJ_RS00700 0.32 -0.3 137,323 + MMJJ_RS00700 0.32 +0.0 137,331 - MMJJ_RS00700 0.35 +0.9 137,332 + MMJJ_RS00700 0.35 -0.1 137,460 - MMJJ_RS00700 0.75 +0.4 137,505 + MMJJ_RS00700 0.90 +0.2 137,517 - +0.5 137,564 + -0.1 137,567 - +0.5 137,568 + +0.4 137,582 + -1.1 137,586 - +0.3 137,587 + -0.4 137,616 - -0.3 137,646 - -0.3 137,690 + MMJJ_RS00705 0.13 +0.2 137,711 - MMJJ_RS00705 0.16 +0.3 137,711 - MMJJ_RS00705 0.16 -0.0 137,712 + MMJJ_RS00705 0.16 -0.6 137,712 + MMJJ_RS00705 0.16 -0.3 137,720 + MMJJ_RS00705 0.17 -0.2 137,720 + MMJJ_RS00705 0.17 +0.4 137,788 + MMJJ_RS00705 0.25 +0.1 137,820 - MMJJ_RS00705 0.29 -0.3 137,820 - MMJJ_RS00705 0.29 +0.6 137,821 + MMJJ_RS00705 0.29 -0.2 137,823 - MMJJ_RS00705 0.29 -0.1 137,823 - MMJJ_RS00705 0.29 +0.3 137,824 + MMJJ_RS00705 0.29 +0.2 137,842 + MMJJ_RS00705 0.32 +0.4 137,849 - MMJJ_RS00705 0.32 +0.1 137,878 + MMJJ_RS00705 0.36 -0.4 137,959 + MMJJ_RS00705 0.46 +0.0 138,015 - MMJJ_RS00705 0.53 +1.3 138,016 + MMJJ_RS00705 0.53 -0.3 138,016 + MMJJ_RS00705 0.53 -0.5 138,016 + MMJJ_RS00705 0.53 -0.6 138,016 + MMJJ_RS00705 0.53 +0.1 138,016 + MMJJ_RS00705 0.53 +0.3 138,150 - MMJJ_RS00705 0.69 -0.4 138,150 - MMJJ_RS00705 0.69 +0.0 138,159 - MMJJ_RS00705 0.70 +0.0 138,160 + MMJJ_RS00705 0.70 -0.1 138,198 + MMJJ_RS00705 0.75 -0.2
Or see this region's nucleotide sequence