Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00535 and MMJJ_RS00540 are separated by 94 nucleotides MMJJ_RS00540 and MMJJ_RS00545 are separated by 33 nucleotides MMJJ_RS00545 and MMJJ_RS00550 are separated by 27 nucleotides
MMJJ_RS00535: MMJJ_RS00535 - tetratricopeptide repeat protein, at 99,700 to 100,881
_RS00535
MMJJ_RS00540: MMJJ_RS00540 - hypothetical protein, at 100,976 to 101,473
_RS00540
MMJJ_RS00545: MMJJ_RS00545 - hypothetical protein, at 101,507 to 102,472
_RS00545
MMJJ_RS00550: MMJJ_RS00550 - AIR synthase-related protein, at 102,500 to 103,381
_RS00550
Position (kb) 101
102
103 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 100.571 kb on + strand, within MMJJ_RS00535 at 100.576 kb on + strand, within MMJJ_RS00535 at 100.593 kb on - strand, within MMJJ_RS00535 at 100.593 kb on - strand, within MMJJ_RS00535 at 100.667 kb on + strand, within MMJJ_RS00535 at 100.693 kb on - strand, within MMJJ_RS00535 at 100.704 kb on + strand, within MMJJ_RS00535 at 100.772 kb on - strand at 100.773 kb on + strand at 100.773 kb on + strand at 100.799 kb on + strand at 100.813 kb on - strand at 100.813 kb on - strand at 100.814 kb on + strand at 100.827 kb on + strand at 100.827 kb on + strand at 100.843 kb on + strand at 100.843 kb on + strand at 100.845 kb on - strand at 100.846 kb on + strand at 100.846 kb on + strand at 100.846 kb on + strand at 100.874 kb on - strand at 100.875 kb on + strand at 100.884 kb on - strand at 100.884 kb on - strand at 100.884 kb on - strand at 100.885 kb on + strand at 100.885 kb on + strand at 100.888 kb on + strand at 100.888 kb on + strand at 100.916 kb on + strand at 100.924 kb on - strand at 100.925 kb on + strand at 100.930 kb on + strand at 100.994 kb on - strand at 100.994 kb on - strand at 100.994 kb on - strand at 100.994 kb on - strand at 100.994 kb on - strand at 100.995 kb on + strand at 100.995 kb on + strand at 101.026 kb on + strand, within MMJJ_RS00540 at 101.037 kb on - strand, within MMJJ_RS00540 at 101.080 kb on + strand, within MMJJ_RS00540 at 101.092 kb on - strand, within MMJJ_RS00540 at 101.092 kb on - strand, within MMJJ_RS00540 at 101.187 kb on - strand, within MMJJ_RS00540 at 101.204 kb on - strand, within MMJJ_RS00540 at 101.268 kb on - strand, within MMJJ_RS00540 at 101.269 kb on + strand, within MMJJ_RS00540 at 101.269 kb on + strand, within MMJJ_RS00540 at 101.269 kb on + strand, within MMJJ_RS00540 at 101.300 kb on - strand, within MMJJ_RS00540 at 101.358 kb on - strand, within MMJJ_RS00540 at 101.364 kb on - strand, within MMJJ_RS00540 at 101.364 kb on - strand, within MMJJ_RS00540 at 101.377 kb on - strand, within MMJJ_RS00540 at 101.438 kb on - strand at 101.481 kb on + strand at 101.496 kb on - strand at 101.545 kb on + strand at 101.611 kb on + strand, within MMJJ_RS00545 at 101.627 kb on + strand, within MMJJ_RS00545 at 101.634 kb on - strand, within MMJJ_RS00545 at 101.662 kb on - strand, within MMJJ_RS00545 at 101.663 kb on + strand, within MMJJ_RS00545 at 101.673 kb on + strand, within MMJJ_RS00545 at 101.702 kb on + strand, within MMJJ_RS00545 at 101.773 kb on + strand, within MMJJ_RS00545 at 101.822 kb on - strand, within MMJJ_RS00545 at 101.854 kb on - strand, within MMJJ_RS00545 at 101.855 kb on + strand, within MMJJ_RS00545 at 101.881 kb on - strand, within MMJJ_RS00545 at 101.932 kb on - strand, within MMJJ_RS00545 at 102.046 kb on - strand, within MMJJ_RS00545 at 102.048 kb on - strand, within MMJJ_RS00545 at 102.112 kb on + strand, within MMJJ_RS00545 at 102.151 kb on - strand, within MMJJ_RS00545 at 102.152 kb on + strand, within MMJJ_RS00545 at 102.157 kb on + strand, within MMJJ_RS00545 at 102.179 kb on + strand, within MMJJ_RS00545 at 102.211 kb on - strand, within MMJJ_RS00545 at 102.211 kb on - strand, within MMJJ_RS00545 at 102.232 kb on - strand, within MMJJ_RS00545 at 102.233 kb on + strand, within MMJJ_RS00545 at 102.233 kb on + strand, within MMJJ_RS00545 at 102.233 kb on + strand, within MMJJ_RS00545 at 102.233 kb on + strand, within MMJJ_RS00545 at 102.235 kb on - strand, within MMJJ_RS00545 at 102.235 kb on - strand, within MMJJ_RS00545 at 102.236 kb on + strand, within MMJJ_RS00545 at 102.283 kb on + strand, within MMJJ_RS00545 at 102.295 kb on + strand, within MMJJ_RS00545 at 102.297 kb on - strand, within MMJJ_RS00545 at 102.297 kb on - strand, within MMJJ_RS00545 at 102.297 kb on - strand, within MMJJ_RS00545 at 102.309 kb on + strand, within MMJJ_RS00545 at 102.315 kb on - strand, within MMJJ_RS00545 at 102.315 kb on - strand, within MMJJ_RS00545 at 102.316 kb on + strand, within MMJJ_RS00545 at 102.326 kb on - strand, within MMJJ_RS00545 at 102.327 kb on + strand, within MMJJ_RS00545 at 102.336 kb on - strand, within MMJJ_RS00545 at 102.337 kb on + strand, within MMJJ_RS00545 at 102.337 kb on + strand, within MMJJ_RS00545 at 102.342 kb on + strand, within MMJJ_RS00545 at 102.342 kb on + strand, within MMJJ_RS00545 at 102.342 kb on + strand, within MMJJ_RS00545 at 102.344 kb on + strand, within MMJJ_RS00545 at 102.368 kb on - strand, within MMJJ_RS00545 at 102.380 kb on - strand at 102.412 kb on - strand at 102.441 kb on - strand at 102.441 kb on - strand at 102.442 kb on + strand at 102.442 kb on + strand at 102.442 kb on + strand at 102.442 kb on + strand at 102.466 kb on + strand at 102.466 kb on + strand at 102.466 kb on + strand at 102.485 kb on - strand at 102.490 kb on + strand at 102.525 kb on + strand at 102.527 kb on - strand at 102.528 kb on + strand at 102.548 kb on + strand at 102.553 kb on - strand at 102.554 kb on + strand at 102.593 kb on - strand, within MMJJ_RS00550 at 102.594 kb on + strand, within MMJJ_RS00550 at 102.612 kb on + strand, within MMJJ_RS00550 at 102.622 kb on - strand, within MMJJ_RS00550 at 102.622 kb on - strand, within MMJJ_RS00550 at 102.622 kb on - strand, within MMJJ_RS00550 at 102.622 kb on - strand, within MMJJ_RS00550 at 102.623 kb on + strand, within MMJJ_RS00550 at 102.646 kb on - strand, within MMJJ_RS00550 at 102.677 kb on - strand, within MMJJ_RS00550 at 102.677 kb on - strand, within MMJJ_RS00550 at 102.681 kb on - strand, within MMJJ_RS00550 at 102.681 kb on - strand, within MMJJ_RS00550 at 102.694 kb on + strand, within MMJJ_RS00550 at 102.694 kb on + strand, within MMJJ_RS00550 at 102.817 kb on - strand, within MMJJ_RS00550 at 102.837 kb on + strand, within MMJJ_RS00550 at 102.884 kb on + strand, within MMJJ_RS00550 at 102.911 kb on - strand, within MMJJ_RS00550 at 102.911 kb on - strand, within MMJJ_RS00550 at 102.912 kb on + strand, within MMJJ_RS00550 at 102.917 kb on - strand, within MMJJ_RS00550 at 102.918 kb on + strand, within MMJJ_RS00550 at 102.965 kb on + strand, within MMJJ_RS00550 at 102.983 kb on - strand, within MMJJ_RS00550 at 102.983 kb on - strand, within MMJJ_RS00550 at 102.984 kb on + strand, within MMJJ_RS00550 at 102.984 kb on + strand, within MMJJ_RS00550 at 102.984 kb on + strand, within MMJJ_RS00550 at 102.984 kb on + strand, within MMJJ_RS00550 at 103.031 kb on - strand, within MMJJ_RS00550 at 103.032 kb on + strand, within MMJJ_RS00550 at 103.046 kb on + strand, within MMJJ_RS00550 at 103.076 kb on - strand, within MMJJ_RS00550 at 103.077 kb on + strand, within MMJJ_RS00550 at 103.128 kb on - strand, within MMJJ_RS00550 at 103.128 kb on - strand, within MMJJ_RS00550 at 103.165 kb on - strand, within MMJJ_RS00550 at 103.165 kb on - strand, within MMJJ_RS00550 at 103.165 kb on - strand, within MMJJ_RS00550 at 103.166 kb on + strand, within MMJJ_RS00550 at 103.166 kb on + strand, within MMJJ_RS00550 at 103.194 kb on + strand, within MMJJ_RS00550 at 103.244 kb on - strand, within MMJJ_RS00550 at 103.245 kb on + strand, within MMJJ_RS00550 at 103.318 kb on - strand at 103.339 kb on - strand at 103.339 kb on - strand at 103.339 kb on - strand at 103.339 kb on - strand at 103.339 kb on - strand at 103.340 kb on + strand at 103.340 kb on + strand at 103.340 kb on + strand at 103.340 kb on + strand at 103.346 kb on - strand at 103.346 kb on - strand at 103.347 kb on + strand at 103.354 kb on + strand at 103.399 kb on - strand at 103.400 kb on + strand at 103.416 kb on + strand at 103.418 kb on + strand at 103.422 kb on - strand at 103.433 kb on + strand at 103.448 kb on - strand at 103.452 kb on - strand at 103.453 kb on + strand at 103.453 kb on + strand at 103.454 kb on - strand at 103.454 kb on - strand at 103.454 kb on - strand at 103.455 kb on + strand at 103.455 kb on + strand at 103.460 kb on - strand at 103.460 kb on - strand at 103.461 kb on + strand at 103.461 kb on + strand at 103.461 kb on + strand at 103.461 kb on + strand at 103.461 kb on + strand at 103.461 kb on + strand at 103.461 kb on + strand at 103.464 kb on - strand at 103.464 kb on - strand at 103.464 kb on - strand at 103.464 kb on - strand at 103.464 kb on - strand at 103.464 kb on - strand at 103.465 kb on + strand at 103.465 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE remove 100,571 + MMJJ_RS00535 0.74 +0.0 100,576 + MMJJ_RS00535 0.74 +0.1 100,593 - MMJJ_RS00535 0.76 -2.4 100,593 - MMJJ_RS00535 0.76 +0.7 100,667 + MMJJ_RS00535 0.82 +0.3 100,693 - MMJJ_RS00535 0.84 +0.7 100,704 + MMJJ_RS00535 0.85 +1.2 100,772 - +0.9 100,773 + +0.7 100,773 + -0.1 100,799 + -0.1 100,813 - -0.7 100,813 - +0.1 100,814 + -0.4 100,827 + +0.0 100,827 + -0.3 100,843 + -0.0 100,843 + -0.3 100,845 - -0.4 100,846 + +1.0 100,846 + -0.1 100,846 + +1.5 100,874 - -0.3 100,875 + -1.0 100,884 - +0.2 100,884 - +1.7 100,884 - +0.8 100,885 + +0.0 100,885 + -0.7 100,888 + +0.1 100,888 + -0.1 100,916 + -0.6 100,924 - -0.1 100,925 + +0.3 100,930 + +0.1 100,994 - +0.9 100,994 - -0.4 100,994 - +0.2 100,994 - +0.5 100,994 - -0.8 100,995 + +0.5 100,995 + -1.3 101,026 + MMJJ_RS00540 0.10 -0.8 101,037 - MMJJ_RS00540 0.12 +0.3 101,080 + MMJJ_RS00540 0.21 +0.2 101,092 - MMJJ_RS00540 0.23 +0.7 101,092 - MMJJ_RS00540 0.23 +0.8 101,187 - MMJJ_RS00540 0.42 -0.5 101,204 - MMJJ_RS00540 0.46 -0.5 101,268 - MMJJ_RS00540 0.59 +0.7 101,269 + MMJJ_RS00540 0.59 -0.3 101,269 + MMJJ_RS00540 0.59 +0.3 101,269 + MMJJ_RS00540 0.59 -0.3 101,300 - MMJJ_RS00540 0.65 -0.1 101,358 - MMJJ_RS00540 0.77 -0.5 101,364 - MMJJ_RS00540 0.78 -0.0 101,364 - MMJJ_RS00540 0.78 +0.1 101,377 - MMJJ_RS00540 0.81 +0.3 101,438 - +0.0 101,481 + -0.4 101,496 - +0.3 101,545 + -0.3 101,611 + MMJJ_RS00545 0.11 -0.0 101,627 + MMJJ_RS00545 0.12 -0.1 101,634 - MMJJ_RS00545 0.13 -0.2 101,662 - MMJJ_RS00545 0.16 -0.2 101,663 + MMJJ_RS00545 0.16 -0.6 101,673 + MMJJ_RS00545 0.17 +0.3 101,702 + MMJJ_RS00545 0.20 +0.9 101,773 + MMJJ_RS00545 0.28 +0.1 101,822 - MMJJ_RS00545 0.33 +0.2 101,854 - MMJJ_RS00545 0.36 -0.2 101,855 + MMJJ_RS00545 0.36 -0.2 101,881 - MMJJ_RS00545 0.39 +0.1 101,932 - MMJJ_RS00545 0.44 -0.5 102,046 - MMJJ_RS00545 0.56 -0.1 102,048 - MMJJ_RS00545 0.56 -0.1 102,112 + MMJJ_RS00545 0.63 -0.9 102,151 - MMJJ_RS00545 0.67 +0.7 102,152 + MMJJ_RS00545 0.67 -0.1 102,157 + MMJJ_RS00545 0.67 -0.4 102,179 + MMJJ_RS00545 0.70 +0.2 102,211 - MMJJ_RS00545 0.73 +0.1 102,211 - MMJJ_RS00545 0.73 +0.1 102,232 - MMJJ_RS00545 0.75 -1.9 102,233 + MMJJ_RS00545 0.75 -0.2 102,233 + MMJJ_RS00545 0.75 -0.4 102,233 + MMJJ_RS00545 0.75 +0.1 102,233 + MMJJ_RS00545 0.75 +0.0 102,235 - MMJJ_RS00545 0.75 -0.8 102,235 - MMJJ_RS00545 0.75 +0.2 102,236 + MMJJ_RS00545 0.75 -0.4 102,283 + MMJJ_RS00545 0.80 +0.9 102,295 + MMJJ_RS00545 0.82 -0.2 102,297 - MMJJ_RS00545 0.82 +0.6 102,297 - MMJJ_RS00545 0.82 +0.3 102,297 - MMJJ_RS00545 0.82 +0.2 102,309 + MMJJ_RS00545 0.83 -0.1 102,315 - MMJJ_RS00545 0.84 -0.0 102,315 - MMJJ_RS00545 0.84 +0.2 102,316 + MMJJ_RS00545 0.84 +0.2 102,326 - MMJJ_RS00545 0.85 -0.0 102,327 + MMJJ_RS00545 0.85 -0.5 102,336 - MMJJ_RS00545 0.86 -0.1 102,337 + MMJJ_RS00545 0.86 +0.5 102,337 + MMJJ_RS00545 0.86 -0.2 102,342 + MMJJ_RS00545 0.86 -0.2 102,342 + MMJJ_RS00545 0.86 -0.5 102,342 + MMJJ_RS00545 0.86 +0.1 102,344 + MMJJ_RS00545 0.87 +0.3 102,368 - MMJJ_RS00545 0.89 -0.3 102,380 - -0.1 102,412 - +0.2 102,441 - +0.2 102,441 - -0.4 102,442 + -0.0 102,442 + -0.3 102,442 + +0.0 102,442 + +0.1 102,466 + +0.1 102,466 + +0.1 102,466 + +0.1 102,485 - -0.2 102,490 + +0.4 102,525 + +1.2 102,527 - -1.1 102,528 + -0.3 102,548 + -1.6 102,553 - -2.2 102,554 + -2.1 102,593 - MMJJ_RS00550 0.11 -1.0 102,594 + MMJJ_RS00550 0.11 -1.4 102,612 + MMJJ_RS00550 0.13 -1.8 102,622 - MMJJ_RS00550 0.14 -1.7 102,622 - MMJJ_RS00550 0.14 -0.4 102,622 - MMJJ_RS00550 0.14 -1.0 102,622 - MMJJ_RS00550 0.14 -3.8 102,623 + MMJJ_RS00550 0.14 -0.1 102,646 - MMJJ_RS00550 0.17 -4.0 102,677 - MMJJ_RS00550 0.20 -0.1 102,677 - MMJJ_RS00550 0.20 -3.2 102,681 - MMJJ_RS00550 0.21 -1.9 102,681 - MMJJ_RS00550 0.21 -0.9 102,694 + MMJJ_RS00550 0.22 -2.2 102,694 + MMJJ_RS00550 0.22 -1.7 102,817 - MMJJ_RS00550 0.36 -4.6 102,837 + MMJJ_RS00550 0.38 -1.5 102,884 + MMJJ_RS00550 0.44 -3.3 102,911 - MMJJ_RS00550 0.47 -0.3 102,911 - MMJJ_RS00550 0.47 -2.4 102,912 + MMJJ_RS00550 0.47 -2.8 102,917 - MMJJ_RS00550 0.47 -1.1 102,918 + MMJJ_RS00550 0.47 -2.2 102,965 + MMJJ_RS00550 0.53 -1.8 102,983 - MMJJ_RS00550 0.55 -1.8 102,983 - MMJJ_RS00550 0.55 -3.5 102,984 + MMJJ_RS00550 0.55 -2.8 102,984 + MMJJ_RS00550 0.55 -2.4 102,984 + MMJJ_RS00550 0.55 -0.9 102,984 + MMJJ_RS00550 0.55 -1.1 103,031 - MMJJ_RS00550 0.60 -1.7 103,032 + MMJJ_RS00550 0.60 -1.4 103,046 + MMJJ_RS00550 0.62 +0.2 103,076 - MMJJ_RS00550 0.65 -1.5 103,077 + MMJJ_RS00550 0.65 -0.7 103,128 - MMJJ_RS00550 0.71 -3.2 103,128 - MMJJ_RS00550 0.71 -2.0 103,165 - MMJJ_RS00550 0.75 -1.1 103,165 - MMJJ_RS00550 0.75 -0.9 103,165 - MMJJ_RS00550 0.75 +1.0 103,166 + MMJJ_RS00550 0.76 -1.9 103,166 + MMJJ_RS00550 0.76 -3.0 103,194 + MMJJ_RS00550 0.79 -2.2 103,244 - MMJJ_RS00550 0.84 -1.1 103,245 + MMJJ_RS00550 0.84 -0.0 103,318 - -1.3 103,339 - -1.7 103,339 - -2.4 103,339 - -0.1 103,339 - -3.2 103,339 - -1.1 103,340 + -1.3 103,340 + +0.7 103,340 + -1.5 103,340 + -2.2 103,346 - -2.5 103,346 - -1.6 103,347 + -1.9 103,354 + -3.1 103,399 - -0.5 103,400 + -0.7 103,416 + -1.2 103,418 + -0.4 103,422 - +0.1 103,433 + -0.0 103,448 - +0.5 103,452 - +0.5 103,453 + -1.1 103,453 + +0.2 103,454 - +0.0 103,454 - -0.7 103,454 - -0.1 103,455 + +0.0 103,455 + +0.0 103,460 - +0.2 103,460 - -0.4 103,461 + +0.2 103,461 + -0.3 103,461 + -0.6 103,461 + +0.6 103,461 + -0.0 103,461 + -0.0 103,461 + -0.5 103,464 - +0.3 103,464 - -1.1 103,464 - +0.1 103,464 - +1.7 103,464 - -0.2 103,464 - -0.2 103,465 + -0.1 103,465 + -0.1
Or see this region's nucleotide sequence