Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS00115

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS00110 and MMJJ_RS00115 are separated by 28 nucleotidesMMJJ_RS00115 and MMJJ_RS00120 are separated by 33 nucleotides MMJJ_RS00110: MMJJ_RS00110 - ATP-dependent DNA ligase, at 20,722 to 22,443 _RS00110 MMJJ_RS00115: MMJJ_RS00115 - hypothetical protein, at 22,472 to 22,888 _RS00115 MMJJ_RS00120: MMJJ_RS00120 - histidinol dehydrogenase, at 22,922 to 24,196 _RS00120 Position (kb) 22 23Strain fitness (log2 ratio) -2 -1 0 1at 21.711 kb on - strand, within MMJJ_RS00110at 21.867 kb on + strand, within MMJJ_RS00110at 21.868 kb on - strand, within MMJJ_RS00110at 22.474 kb on + strandat 22.474 kb on + strandat 22.474 kb on + strandat 22.492 kb on - strandat 22.496 kb on - strandat 22.623 kb on + strand, within MMJJ_RS00115at 22.623 kb on + strand, within MMJJ_RS00115at 22.626 kb on - strand, within MMJJ_RS00115at 22.626 kb on - strand, within MMJJ_RS00115at 22.626 kb on - strand, within MMJJ_RS00115at 22.627 kb on + strand, within MMJJ_RS00115at 22.627 kb on + strand, within MMJJ_RS00115at 22.693 kb on - strand, within MMJJ_RS00115at 22.714 kb on - strand, within MMJJ_RS00115at 22.814 kb on + strand, within MMJJ_RS00115at 22.865 kb on + strandat 22.868 kb on - strandat 22.869 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE
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21,711 - MMJJ_RS00110 0.57 -1.1
21,867 + MMJJ_RS00110 0.66 -0.9
21,868 - MMJJ_RS00110 0.67 -1.1
22,474 + +0.5
22,474 + +0.8
22,474 + -2.1
22,492 - +0.3
22,496 - -0.7
22,623 + MMJJ_RS00115 0.36 +0.3
22,623 + MMJJ_RS00115 0.36 +0.3
22,626 - MMJJ_RS00115 0.37 +0.3
22,626 - MMJJ_RS00115 0.37 +0.0
22,626 - MMJJ_RS00115 0.37 -0.1
22,627 + MMJJ_RS00115 0.37 +0.8
22,627 + MMJJ_RS00115 0.37 +0.7
22,693 - MMJJ_RS00115 0.53 +0.1
22,714 - MMJJ_RS00115 0.58 -1.0
22,814 + MMJJ_RS00115 0.82 +0.4
22,865 + -0.1
22,868 - -0.1
22,869 + -0.1

Or see this region's nucleotide sequence