Experiment: R2A with Chromium(III) Chloride Hexahydrate 0.15625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS09160 and LRK54_RS09165 are separated by 509 nucleotides LRK54_RS09165 and LRK54_RS09170 are separated by 96 nucleotides
LRK54_RS09160: LRK54_RS09160 - arginine--tRNA ligase, at 2,005,936 to 2,007,624
_RS09160
LRK54_RS09165: LRK54_RS09165 - DNA repair protein RadC, at 2,008,134 to 2,008,805
_RS09165
LRK54_RS09170: LRK54_RS09170 - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC, at 2,008,902 to 2,010,107
_RS09170
Position (kb)
2008
2009 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2007.660 kb on + strand at 2007.660 kb on + strand at 2007.660 kb on + strand at 2007.661 kb on - strand at 2007.661 kb on - strand at 2007.661 kb on - strand at 2007.773 kb on - strand at 2007.773 kb on - strand at 2007.773 kb on - strand at 2007.773 kb on - strand at 2007.773 kb on - strand at 2007.773 kb on - strand at 2007.773 kb on - strand at 2007.773 kb on - strand at 2007.832 kb on + strand at 2007.832 kb on + strand at 2007.832 kb on + strand at 2007.832 kb on + strand at 2007.832 kb on + strand at 2007.833 kb on - strand at 2007.833 kb on - strand at 2007.850 kb on - strand at 2007.850 kb on - strand at 2007.899 kb on + strand at 2007.919 kb on - strand at 2007.933 kb on + strand at 2007.933 kb on + strand at 2007.933 kb on + strand at 2007.933 kb on + strand at 2007.933 kb on + strand at 2007.933 kb on + strand at 2007.933 kb on + strand at 2007.933 kb on + strand at 2007.933 kb on + strand at 2007.934 kb on - strand at 2007.934 kb on - strand at 2007.934 kb on - strand at 2007.934 kb on - strand at 2007.934 kb on - strand at 2007.934 kb on - strand at 2007.934 kb on - strand at 2007.934 kb on - strand at 2007.934 kb on - strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.935 kb on + strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2007.936 kb on - strand at 2008.005 kb on + strand at 2008.005 kb on + strand at 2008.006 kb on - strand at 2008.006 kb on - strand at 2008.423 kb on + strand, within LRK54_RS09165 at 2008.423 kb on + strand, within LRK54_RS09165 at 2008.423 kb on + strand, within LRK54_RS09165 at 2008.423 kb on + strand, within LRK54_RS09165 at 2008.423 kb on + strand, within LRK54_RS09165 at 2008.423 kb on + strand, within LRK54_RS09165 at 2008.423 kb on + strand, within LRK54_RS09165 at 2008.424 kb on - strand, within LRK54_RS09165 at 2008.424 kb on - strand, within LRK54_RS09165 at 2008.424 kb on - strand, within LRK54_RS09165 at 2008.424 kb on - strand, within LRK54_RS09165 at 2008.424 kb on - strand, within LRK54_RS09165 at 2008.424 kb on - strand, within LRK54_RS09165 at 2008.424 kb on - strand, within LRK54_RS09165 at 2008.424 kb on - strand, within LRK54_RS09165 at 2008.424 kb on - strand, within LRK54_RS09165 at 2008.424 kb on - strand, within LRK54_RS09165 at 2008.444 kb on + strand, within LRK54_RS09165 at 2008.444 kb on + strand, within LRK54_RS09165 at 2008.444 kb on + strand, within LRK54_RS09165 at 2008.444 kb on + strand, within LRK54_RS09165 at 2008.444 kb on + strand, within LRK54_RS09165 at 2008.444 kb on + strand, within LRK54_RS09165 at 2008.444 kb on + strand, within LRK54_RS09165 at 2008.445 kb on - strand, within LRK54_RS09165 at 2008.445 kb on - strand, within LRK54_RS09165 at 2008.445 kb on - strand, within LRK54_RS09165 at 2008.445 kb on - strand, within LRK54_RS09165 at 2008.445 kb on - strand, within LRK54_RS09165 at 2008.445 kb on - strand, within LRK54_RS09165 at 2008.474 kb on + strand, within LRK54_RS09165 at 2008.474 kb on + strand, within LRK54_RS09165 at 2008.474 kb on + strand, within LRK54_RS09165 at 2008.474 kb on + strand, within LRK54_RS09165 at 2008.474 kb on - strand, within LRK54_RS09165 at 2008.475 kb on - strand, within LRK54_RS09165 at 2008.475 kb on - strand, within LRK54_RS09165 at 2008.475 kb on - strand, within LRK54_RS09165 at 2008.475 kb on - strand, within LRK54_RS09165 at 2008.475 kb on - strand, within LRK54_RS09165 at 2008.475 kb on - strand, within LRK54_RS09165 at 2008.475 kb on - strand, within LRK54_RS09165 at 2008.710 kb on + strand, within LRK54_RS09165 at 2008.710 kb on + strand, within LRK54_RS09165 at 2008.710 kb on + strand, within LRK54_RS09165 at 2008.711 kb on - strand, within LRK54_RS09165 at 2008.864 kb on + strand at 2008.864 kb on + strand at 2008.864 kb on + strand at 2008.865 kb on - strand at 2008.865 kb on - strand at 2008.865 kb on - strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.869 kb on + strand at 2008.899 kb on + strand at 2008.899 kb on + strand at 2008.899 kb on + strand at 2008.899 kb on + strand at 2008.899 kb on + strand at 2008.899 kb on + strand at 2008.899 kb on + strand at 2008.899 kb on + strand at 2008.899 kb on + strand at 2008.899 kb on + strand at 2008.899 kb on + strand at 2008.899 kb on + strand at 2008.899 kb on + strand at 2008.899 kb on + strand at 2008.899 kb on + strand at 2008.899 kb on + strand at 2008.899 kb on + strand at 2008.899 kb on + strand at 2008.899 kb on + strand at 2008.899 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chromium(III) Chloride Hexahydrate 0.15625 mM remove 2,007,660 + +0.9 2,007,660 + -1.2 2,007,660 + -1.0 2,007,661 - +0.4 2,007,661 - -1.4 2,007,661 - -0.4 2,007,773 - -0.7 2,007,773 - -1.2 2,007,773 - +1.3 2,007,773 - +1.4 2,007,773 - +0.5 2,007,773 - +1.1 2,007,773 - -0.2 2,007,773 - +0.5 2,007,832 + -0.4 2,007,832 + -0.5 2,007,832 + +0.9 2,007,832 + +1.1 2,007,832 + -0.5 2,007,833 - -0.7 2,007,833 - -0.7 2,007,850 - +0.2 2,007,850 - -0.7 2,007,899 + -0.2 2,007,919 - +0.2 2,007,933 + -0.9 2,007,933 + +1.1 2,007,933 + -0.9 2,007,933 + +0.9 2,007,933 + +0.1 2,007,933 + -1.2 2,007,933 + +1.6 2,007,933 + -1.3 2,007,933 + -0.4 2,007,934 - +0.5 2,007,934 - -1.1 2,007,934 - +0.1 2,007,934 - -0.5 2,007,934 - +0.1 2,007,934 - +0.3 2,007,934 - +0.2 2,007,934 - +0.9 2,007,934 - -0.7 2,007,935 + -0.8 2,007,935 + +1.5 2,007,935 + +0.6 2,007,935 + +0.0 2,007,935 + -0.3 2,007,935 + -0.0 2,007,935 + -0.2 2,007,935 + +1.8 2,007,935 + +0.3 2,007,935 + -2.8 2,007,935 + -0.7 2,007,935 + +0.2 2,007,935 + +2.2 2,007,935 + +0.5 2,007,935 + -1.6 2,007,935 + -2.1 2,007,935 + +0.1 2,007,935 + -0.6 2,007,935 + -0.6 2,007,935 + -0.9 2,007,935 + +2.5 2,007,935 + +0.5 2,007,935 + -0.3 2,007,935 + -0.1 2,007,936 - -1.3 2,007,936 - +0.5 2,007,936 - -0.2 2,007,936 - +1.1 2,007,936 - +0.3 2,007,936 - +0.9 2,007,936 - +0.6 2,007,936 - +1.5 2,007,936 - +0.5 2,007,936 - +0.5 2,007,936 - -1.4 2,007,936 - +2.5 2,007,936 - +1.1 2,007,936 - +0.5 2,007,936 - -0.8 2,007,936 - +1.1 2,007,936 - +0.5 2,007,936 - +0.8 2,007,936 - -2.1 2,007,936 - -0.5 2,007,936 - +0.1 2,007,936 - -0.2 2,007,936 - -0.2 2,008,005 + +0.5 2,008,005 + +1.2 2,008,006 - +0.9 2,008,006 - -3.3 2,008,423 + LRK54_RS09165 0.43 +0.2 2,008,423 + LRK54_RS09165 0.43 -0.2 2,008,423 + LRK54_RS09165 0.43 +0.1 2,008,423 + LRK54_RS09165 0.43 -0.7 2,008,423 + LRK54_RS09165 0.43 -1.4 2,008,423 + LRK54_RS09165 0.43 +0.9 2,008,423 + LRK54_RS09165 0.43 -0.6 2,008,424 - LRK54_RS09165 0.43 +0.3 2,008,424 - LRK54_RS09165 0.43 +0.4 2,008,424 - LRK54_RS09165 0.43 +1.3 2,008,424 - LRK54_RS09165 0.43 +0.8 2,008,424 - LRK54_RS09165 0.43 +0.6 2,008,424 - LRK54_RS09165 0.43 +0.7 2,008,424 - LRK54_RS09165 0.43 +0.2 2,008,424 - LRK54_RS09165 0.43 -0.9 2,008,424 - LRK54_RS09165 0.43 -1.3 2,008,424 - LRK54_RS09165 0.43 -0.9 2,008,444 + LRK54_RS09165 0.46 -0.3 2,008,444 + LRK54_RS09165 0.46 -0.4 2,008,444 + LRK54_RS09165 0.46 -0.3 2,008,444 + LRK54_RS09165 0.46 -0.7 2,008,444 + LRK54_RS09165 0.46 -0.9 2,008,444 + LRK54_RS09165 0.46 -2.8 2,008,444 + LRK54_RS09165 0.46 -0.4 2,008,445 - LRK54_RS09165 0.46 -1.4 2,008,445 - LRK54_RS09165 0.46 +0.8 2,008,445 - LRK54_RS09165 0.46 -1.2 2,008,445 - LRK54_RS09165 0.46 -1.4 2,008,445 - LRK54_RS09165 0.46 -0.7 2,008,445 - LRK54_RS09165 0.46 -1.4 2,008,474 + LRK54_RS09165 0.51 -0.1 2,008,474 + LRK54_RS09165 0.51 +0.3 2,008,474 + LRK54_RS09165 0.51 -0.6 2,008,474 + LRK54_RS09165 0.51 -1.9 2,008,474 - LRK54_RS09165 0.51 +0.1 2,008,475 - LRK54_RS09165 0.51 -1.3 2,008,475 - LRK54_RS09165 0.51 +0.4 2,008,475 - LRK54_RS09165 0.51 +2.0 2,008,475 - LRK54_RS09165 0.51 -0.7 2,008,475 - LRK54_RS09165 0.51 -1.0 2,008,475 - LRK54_RS09165 0.51 -0.4 2,008,475 - LRK54_RS09165 0.51 +0.0 2,008,710 + LRK54_RS09165 0.86 -0.0 2,008,710 + LRK54_RS09165 0.86 +0.9 2,008,710 + LRK54_RS09165 0.86 +1.1 2,008,711 - LRK54_RS09165 0.86 +0.1 2,008,864 + -0.5 2,008,864 + +0.2 2,008,864 + -0.3 2,008,865 - +0.1 2,008,865 - -0.2 2,008,865 - -2.1 2,008,869 + +0.3 2,008,869 + +0.3 2,008,869 + +1.5 2,008,869 + -1.2 2,008,869 + -0.2 2,008,869 + +0.8 2,008,869 + +1.7 2,008,869 + +0.1 2,008,869 + -1.2 2,008,869 + +0.1 2,008,869 + +0.9 2,008,869 + +1.2 2,008,869 + +0.5 2,008,869 + -0.1 2,008,869 + +0.1 2,008,869 + +0.9 2,008,869 + -0.4 2,008,869 + -0.3 2,008,869 + -0.7 2,008,869 + -0.4 2,008,869 + -1.2 2,008,899 + -0.1 2,008,899 + +1.0 2,008,899 + -1.2 2,008,899 + -0.6 2,008,899 + -0.3 2,008,899 + -0.1 2,008,899 + +0.9 2,008,899 + +0.2 2,008,899 + -0.7 2,008,899 + -0.7 2,008,899 + +2.2 2,008,899 + -1.1 2,008,899 + -0.3 2,008,899 + +2.5 2,008,899 + +0.2 2,008,899 + +0.7 2,008,899 + -0.8 2,008,899 + +0.4 2,008,899 + -1.3 2,008,899 + +3.5
Or see this region's nucleotide sequence