Experiment: A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS06710 and HSERO_RS06715 are separated by 55 nucleotides HSERO_RS06715 and HSERO_RS06720 are separated by 5 nucleotides HSERO_RS06720 and HSERO_RS06725 are separated by 81 nucleotides
HSERO_RS06710: HSERO_RS06710 - ferredoxin, at 1,526,061 to 1,527,017
_RS06710
HSERO_RS06715: HSERO_RS06715 - aromatic-ring-hydroxylating dioxygenase subunit beta, at 1,527,073 to 1,527,573
_RS06715
HSERO_RS06720: HSERO_RS06720 - Rieske (2Fe-2S) protein, at 1,527,579 to 1,528,865
_RS06720
HSERO_RS06725: HSERO_RS06725 - glutamyl-tRNA amidotransferase, at 1,528,947 to 1,530,068
_RS06725
Position (kb)
1527
1528
1529 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1526.608 kb on + strand, within HSERO_RS06710 at 1526.610 kb on + strand, within HSERO_RS06710 at 1526.910 kb on - strand, within HSERO_RS06710 at 1527.250 kb on - strand, within HSERO_RS06715 at 1527.396 kb on - strand, within HSERO_RS06715 at 1527.547 kb on + strand at 1528.006 kb on + strand at 1528.393 kb on - strand, within HSERO_RS06720 at 1528.542 kb on - strand, within HSERO_RS06720 at 1528.777 kb on - strand at 1528.777 kb on - strand at 1528.821 kb on + strand at 1528.831 kb on + strand at 1528.849 kb on - strand at 1528.860 kb on + strand at 1528.945 kb on + strand at 1528.990 kb on - strand at 1529.096 kb on + strand, within HSERO_RS06725 at 1529.096 kb on + strand, within HSERO_RS06725 at 1529.104 kb on - strand, within HSERO_RS06725 at 1529.275 kb on - strand, within HSERO_RS06725 at 1529.522 kb on - strand, within HSERO_RS06725 at 1529.596 kb on - strand, within HSERO_RS06725 at 1529.609 kb on + strand, within HSERO_RS06725 at 1529.609 kb on + strand, within HSERO_RS06725 at 1529.628 kb on + strand, within HSERO_RS06725 at 1529.661 kb on - strand, within HSERO_RS06725
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.02 mg/ml remove 1,526,608 + HSERO_RS06710 0.57 -0.4 1,526,610 + HSERO_RS06710 0.57 +0.5 1,526,910 - HSERO_RS06710 0.89 -1.0 1,527,250 - HSERO_RS06715 0.35 +0.0 1,527,396 - HSERO_RS06715 0.64 -1.5 1,527,547 + +0.0 1,528,006 + +1.4 1,528,393 - HSERO_RS06720 0.63 -0.4 1,528,542 - HSERO_RS06720 0.75 +0.1 1,528,777 - +0.1 1,528,777 - +0.2 1,528,821 + +1.2 1,528,831 + -1.6 1,528,849 - -0.8 1,528,860 + +0.4 1,528,945 + +2.0 1,528,990 - -0.3 1,529,096 + HSERO_RS06725 0.13 -0.5 1,529,096 + HSERO_RS06725 0.13 -1.9 1,529,104 - HSERO_RS06725 0.14 +0.1 1,529,275 - HSERO_RS06725 0.29 +0.5 1,529,522 - HSERO_RS06725 0.51 +0.1 1,529,596 - HSERO_RS06725 0.58 -1.0 1,529,609 + HSERO_RS06725 0.59 -0.2 1,529,609 + HSERO_RS06725 0.59 +2.2 1,529,628 + HSERO_RS06725 0.61 +0.3 1,529,661 - HSERO_RS06725 0.64 -0.4
Or see this region's nucleotide sequence