Experiment: A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS03480 and HSERO_RS03485 are separated by 85 nucleotides HSERO_RS03485 and HSERO_RS03490 are separated by 70 nucleotides HSERO_RS03490 and HSERO_RS03495 overlap by 4 nucleotides HSERO_RS03495 and HSERO_RS03500 are separated by 90 nucleotides HSERO_RS03500 and HSERO_RS03505 are separated by 123 nucleotides
HSERO_RS03480: HSERO_RS03480 - NAD(P) transhydrogenase subunit alpha, at 757,691 to 758,806
_RS03480
HSERO_RS03485: HSERO_RS03485 - aspartate decarboxylase, at 758,892 to 759,254
_RS03485
HSERO_RS03490: HSERO_RS03490 - NAD(P) transhydrogenase, at 759,325 to 759,639
_RS03490
HSERO_RS03495: HSERO_RS03495 - NAD(P) transhydrogenase subunit beta, at 759,636 to 761,075
_RS03495
HSERO_RS03500: HSERO_RS03500 - uracil phosphoribosyltransferase, at 761,166 to 761,813
_RS03500
HSERO_RS03505: HSERO_RS03505 - 2-octaprenyl-6-methoxyphenyl hydroxylase, at 761,937 to 763,085
_RS03505
Position (kb) 759
760
761
762 Strain fitness (log2 ratio) -2
-1
0
1 at 758.653 kb on + strand, within HSERO_RS03480 at 758.743 kb on - strand at 758.859 kb on - strand at 759.343 kb on - strand at 759.482 kb on + strand, within HSERO_RS03490 at 759.615 kb on + strand at 759.657 kb on + strand at 759.710 kb on - strand at 759.797 kb on + strand, within HSERO_RS03495 at 760.004 kb on + strand, within HSERO_RS03495 at 760.086 kb on - strand, within HSERO_RS03495 at 760.142 kb on + strand, within HSERO_RS03495 at 760.142 kb on + strand, within HSERO_RS03495 at 760.142 kb on + strand, within HSERO_RS03495 at 760.142 kb on + strand, within HSERO_RS03495 at 760.414 kb on - strand, within HSERO_RS03495 at 760.456 kb on - strand, within HSERO_RS03495 at 760.635 kb on - strand, within HSERO_RS03495 at 760.640 kb on - strand, within HSERO_RS03495 at 760.771 kb on - strand, within HSERO_RS03495 at 760.816 kb on - strand, within HSERO_RS03495 at 760.829 kb on + strand, within HSERO_RS03495 at 760.873 kb on - strand, within HSERO_RS03495 at 760.939 kb on - strand at 761.074 kb on + strand at 761.334 kb on - strand, within HSERO_RS03500 at 761.342 kb on + strand, within HSERO_RS03500 at 761.457 kb on + strand, within HSERO_RS03500 at 761.662 kb on - strand, within HSERO_RS03500 at 761.786 kb on + strand at 761.829 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.02 mg/ml remove 758,653 + HSERO_RS03480 0.86 -0.8 758,743 - -1.3 758,859 - -1.6 759,343 - +0.4 759,482 + HSERO_RS03490 0.50 +0.7 759,615 + -0.6 759,657 + -0.2 759,710 - -1.2 759,797 + HSERO_RS03495 0.11 -0.2 760,004 + HSERO_RS03495 0.26 +0.3 760,086 - HSERO_RS03495 0.31 -1.6 760,142 + HSERO_RS03495 0.35 -0.3 760,142 + HSERO_RS03495 0.35 -0.0 760,142 + HSERO_RS03495 0.35 -0.2 760,142 + HSERO_RS03495 0.35 -0.4 760,414 - HSERO_RS03495 0.54 +0.3 760,456 - HSERO_RS03495 0.57 -1.5 760,635 - HSERO_RS03495 0.69 -1.2 760,640 - HSERO_RS03495 0.70 -1.0 760,771 - HSERO_RS03495 0.79 -0.6 760,816 - HSERO_RS03495 0.82 -1.5 760,829 + HSERO_RS03495 0.83 +0.1 760,873 - HSERO_RS03495 0.86 -0.2 760,939 - +1.2 761,074 + +0.5 761,334 - HSERO_RS03500 0.26 -0.8 761,342 + HSERO_RS03500 0.27 +0.3 761,457 + HSERO_RS03500 0.45 +0.4 761,662 - HSERO_RS03500 0.77 -1.9 761,786 + +0.2 761,829 + -0.4
Or see this region's nucleotide sequence