Experiment: L-Proline (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_16080 and AO356_16085 overlap by 11 nucleotides AO356_16085 and AO356_16090 are separated by 77 nucleotides
AO356_16080: AO356_16080 - ATPase, at 3,637,432 to 3,640,380
_16080
AO356_16085: AO356_16085 - MFS transporter, at 3,640,370 to 3,640,546
_16085
AO356_16090: AO356_16090 - glutamyl-Q tRNA(Asp) ligase, at 3,640,624 to 3,641,505
_16090
Position (kb)
3640
3641 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3639.407 kb on + strand, within AO356_16080 at 3639.407 kb on + strand, within AO356_16080 at 3639.408 kb on - strand, within AO356_16080 at 3639.611 kb on + strand, within AO356_16080 at 3639.611 kb on + strand, within AO356_16080 at 3639.611 kb on + strand, within AO356_16080 at 3639.611 kb on + strand, within AO356_16080 at 3639.611 kb on + strand, within AO356_16080 at 3639.611 kb on + strand, within AO356_16080 at 3639.611 kb on + strand, within AO356_16080 at 3639.611 kb on + strand, within AO356_16080 at 3639.611 kb on + strand, within AO356_16080 at 3639.611 kb on + strand, within AO356_16080 at 3639.611 kb on + strand, within AO356_16080 at 3639.611 kb on + strand, within AO356_16080 at 3639.612 kb on - strand, within AO356_16080 at 3639.612 kb on - strand, within AO356_16080 at 3639.612 kb on - strand, within AO356_16080 at 3639.612 kb on - strand, within AO356_16080 at 3639.612 kb on - strand, within AO356_16080 at 3639.612 kb on - strand, within AO356_16080 at 3639.612 kb on - strand, within AO356_16080 at 3639.612 kb on - strand, within AO356_16080 at 3639.612 kb on - strand, within AO356_16080 at 3639.761 kb on + strand, within AO356_16080 at 3639.770 kb on + strand, within AO356_16080 at 3639.770 kb on + strand, within AO356_16080 at 3639.771 kb on - strand, within AO356_16080 at 3639.771 kb on - strand, within AO356_16080 at 3639.976 kb on + strand, within AO356_16080 at 3639.976 kb on + strand, within AO356_16080 at 3639.977 kb on - strand, within AO356_16080 at 3639.977 kb on - strand, within AO356_16080 at 3639.977 kb on - strand, within AO356_16080 at 3640.091 kb on + strand at 3640.091 kb on + strand at 3640.092 kb on - strand at 3640.092 kb on - strand at 3640.092 kb on - strand at 3640.153 kb on + strand at 3640.182 kb on - strand at 3640.188 kb on - strand at 3640.193 kb on + strand at 3640.193 kb on + strand at 3640.193 kb on + strand at 3640.194 kb on - strand at 3640.194 kb on - strand at 3640.194 kb on - strand at 3640.214 kb on + strand at 3640.214 kb on + strand at 3640.214 kb on + strand at 3640.214 kb on + strand at 3640.214 kb on + strand at 3640.214 kb on + strand at 3640.214 kb on + strand at 3640.214 kb on + strand at 3640.214 kb on + strand at 3640.215 kb on - strand at 3640.215 kb on - strand at 3640.215 kb on - strand at 3640.235 kb on + strand at 3640.235 kb on + strand at 3640.235 kb on + strand at 3640.235 kb on + strand at 3640.236 kb on - strand at 3640.236 kb on - strand at 3640.236 kb on - strand at 3640.236 kb on - strand at 3640.236 kb on - strand at 3640.256 kb on + strand at 3640.256 kb on + strand at 3640.257 kb on - strand at 3640.257 kb on - strand at 3640.257 kb on - strand at 3640.257 kb on - strand at 3640.257 kb on - strand at 3640.257 kb on - strand at 3640.257 kb on - strand at 3640.257 kb on - strand at 3640.257 kb on - strand at 3640.337 kb on + strand at 3640.371 kb on + strand at 3640.440 kb on + strand, within AO356_16085 at 3640.440 kb on + strand, within AO356_16085 at 3640.441 kb on - strand, within AO356_16085 at 3640.503 kb on + strand, within AO356_16085 at 3640.503 kb on + strand, within AO356_16085 at 3640.503 kb on + strand, within AO356_16085 at 3640.503 kb on + strand, within AO356_16085 at 3640.503 kb on + strand, within AO356_16085 at 3640.504 kb on - strand, within AO356_16085 at 3640.504 kb on - strand, within AO356_16085 at 3640.504 kb on - strand, within AO356_16085 at 3640.504 kb on - strand, within AO356_16085 at 3640.514 kb on + strand, within AO356_16085 at 3640.536 kb on + strand at 3640.536 kb on + strand at 3640.536 kb on + strand at 3640.536 kb on + strand at 3640.536 kb on + strand at 3640.536 kb on + strand at 3640.537 kb on - strand at 3640.537 kb on - strand at 3640.537 kb on - strand at 3640.537 kb on - strand at 3640.537 kb on - strand at 3640.537 kb on - strand at 3640.540 kb on + strand at 3640.540 kb on + strand at 3640.540 kb on + strand at 3640.540 kb on + strand at 3640.540 kb on + strand at 3640.540 kb on + strand at 3640.540 kb on + strand at 3640.540 kb on + strand at 3640.540 kb on + strand at 3640.540 kb on + strand at 3640.540 kb on + strand at 3640.540 kb on + strand at 3640.540 kb on + strand at 3640.540 kb on + strand at 3640.540 kb on + strand at 3640.540 kb on + strand at 3640.540 kb on + strand at 3640.540 kb on + strand at 3640.540 kb on + strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.541 kb on - strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.542 kb on + strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.543 kb on - strand at 3640.558 kb on + strand at 3640.559 kb on - strand at 3640.559 kb on - strand at 3640.559 kb on - strand at 3640.559 kb on - strand at 3640.563 kb on + strand at 3640.630 kb on + strand at 3640.630 kb on + strand at 3640.630 kb on + strand at 3640.630 kb on + strand at 3640.631 kb on - strand at 3640.631 kb on - strand at 3640.631 kb on - strand at 3640.631 kb on - strand at 3640.631 kb on - strand at 3640.814 kb on + strand, within AO356_16090 at 3640.815 kb on - strand, within AO356_16090 at 3640.904 kb on + strand, within AO356_16090 at 3640.904 kb on + strand, within AO356_16090 at 3640.905 kb on - strand, within AO356_16090 at 3640.905 kb on - strand, within AO356_16090 at 3640.905 kb on - strand, within AO356_16090 at 3640.905 kb on - strand, within AO356_16090 at 3640.997 kb on + strand, within AO356_16090 at 3640.997 kb on + strand, within AO356_16090 at 3640.998 kb on - strand, within AO356_16090 at 3641.003 kb on + strand, within AO356_16090 at 3641.003 kb on + strand, within AO356_16090 at 3641.003 kb on + strand, within AO356_16090 at 3641.003 kb on + strand, within AO356_16090 at 3641.003 kb on + strand, within AO356_16090 at 3641.003 kb on + strand, within AO356_16090 at 3641.003 kb on + strand, within AO356_16090 at 3641.003 kb on + strand, within AO356_16090 at 3641.003 kb on + strand, within AO356_16090 at 3641.003 kb on + strand, within AO356_16090 at 3641.003 kb on + strand, within AO356_16090 at 3641.003 kb on + strand, within AO356_16090 at 3641.004 kb on - strand, within AO356_16090 at 3641.004 kb on - strand, within AO356_16090 at 3641.004 kb on - strand, within AO356_16090 at 3641.004 kb on - strand, within AO356_16090 at 3641.004 kb on - strand, within AO356_16090 at 3641.004 kb on - strand, within AO356_16090 at 3641.004 kb on - strand, within AO356_16090 at 3641.004 kb on - strand, within AO356_16090 at 3641.093 kb on + strand, within AO356_16090 at 3641.093 kb on + strand, within AO356_16090 at 3641.094 kb on - strand, within AO356_16090 at 3641.094 kb on - strand, within AO356_16090 at 3641.180 kb on + strand, within AO356_16090 at 3641.181 kb on - strand, within AO356_16090 at 3641.181 kb on - strand, within AO356_16090 at 3641.181 kb on - strand, within AO356_16090 at 3641.216 kb on + strand, within AO356_16090 at 3641.216 kb on + strand, within AO356_16090 at 3641.217 kb on - strand, within AO356_16090 at 3641.217 kb on - strand, within AO356_16090 at 3641.312 kb on + strand, within AO356_16090 at 3641.313 kb on - strand, within AO356_16090 at 3641.313 kb on - strand, within AO356_16090 at 3641.423 kb on + strand at 3641.423 kb on + strand at 3641.423 kb on + strand at 3641.423 kb on + strand at 3641.423 kb on + strand at 3641.423 kb on + strand at 3641.423 kb on + strand at 3641.424 kb on - strand at 3641.424 kb on - strand at 3641.424 kb on - strand at 3641.424 kb on - strand at 3641.495 kb on + strand at 3641.496 kb on - strand at 3641.496 kb on - strand at 3641.496 kb on - strand at 3641.505 kb on + strand at 3641.506 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (C) remove 3,639,407 + AO356_16080 0.67 -1.5 3,639,407 + AO356_16080 0.67 -1.0 3,639,408 - AO356_16080 0.67 -1.2 3,639,611 + AO356_16080 0.74 -1.7 3,639,611 + AO356_16080 0.74 -1.7 3,639,611 + AO356_16080 0.74 -0.5 3,639,611 + AO356_16080 0.74 -2.6 3,639,611 + AO356_16080 0.74 -2.0 3,639,611 + AO356_16080 0.74 -2.2 3,639,611 + AO356_16080 0.74 -2.0 3,639,611 + AO356_16080 0.74 -2.1 3,639,611 + AO356_16080 0.74 -2.1 3,639,611 + AO356_16080 0.74 -1.8 3,639,611 + AO356_16080 0.74 -1.8 3,639,611 + AO356_16080 0.74 -0.5 3,639,612 - AO356_16080 0.74 -3.3 3,639,612 - AO356_16080 0.74 -4.1 3,639,612 - AO356_16080 0.74 -2.1 3,639,612 - AO356_16080 0.74 -2.1 3,639,612 - AO356_16080 0.74 +0.1 3,639,612 - AO356_16080 0.74 -2.1 3,639,612 - AO356_16080 0.74 -0.0 3,639,612 - AO356_16080 0.74 -1.5 3,639,612 - AO356_16080 0.74 -1.1 3,639,761 + AO356_16080 0.79 -1.0 3,639,770 + AO356_16080 0.79 -2.0 3,639,770 + AO356_16080 0.79 -0.5 3,639,771 - AO356_16080 0.79 -1.8 3,639,771 - AO356_16080 0.79 -0.9 3,639,976 + AO356_16080 0.86 -1.2 3,639,976 + AO356_16080 0.86 -0.9 3,639,977 - AO356_16080 0.86 -2.5 3,639,977 - AO356_16080 0.86 -1.4 3,639,977 - AO356_16080 0.86 -3.3 3,640,091 + -1.2 3,640,091 + -3.1 3,640,092 - -2.7 3,640,092 - -2.7 3,640,092 - -2.8 3,640,153 + -1.0 3,640,182 - -1.9 3,640,188 - -2.7 3,640,193 + -1.7 3,640,193 + -2.6 3,640,193 + -3.2 3,640,194 - -0.9 3,640,194 - -1.3 3,640,194 - -1.5 3,640,214 + -1.6 3,640,214 + -0.9 3,640,214 + -2.5 3,640,214 + -2.9 3,640,214 + -1.6 3,640,214 + -0.4 3,640,214 + -1.2 3,640,214 + -1.5 3,640,214 + -1.9 3,640,215 - -3.6 3,640,215 - -2.3 3,640,215 - -2.6 3,640,235 + -0.7 3,640,235 + -1.8 3,640,235 + -0.2 3,640,235 + -0.5 3,640,236 - -1.9 3,640,236 - -1.7 3,640,236 - -2.7 3,640,236 - -1.6 3,640,236 - -2.8 3,640,256 + -0.9 3,640,256 + +0.7 3,640,257 - -3.0 3,640,257 - -1.9 3,640,257 - -1.1 3,640,257 - -2.5 3,640,257 - -1.0 3,640,257 - +0.5 3,640,257 - -2.0 3,640,257 - -1.3 3,640,257 - -2.4 3,640,337 + -0.5 3,640,371 + +0.0 3,640,440 + AO356_16085 0.40 -2.3 3,640,440 + AO356_16085 0.40 -1.8 3,640,441 - AO356_16085 0.40 -0.8 3,640,503 + AO356_16085 0.75 -3.0 3,640,503 + AO356_16085 0.75 -2.5 3,640,503 + AO356_16085 0.75 -2.0 3,640,503 + AO356_16085 0.75 -1.3 3,640,503 + AO356_16085 0.75 -0.9 3,640,504 - AO356_16085 0.76 -0.7 3,640,504 - AO356_16085 0.76 -1.0 3,640,504 - AO356_16085 0.76 -1.0 3,640,504 - AO356_16085 0.76 -0.3 3,640,514 + AO356_16085 0.81 -2.0 3,640,536 + +0.2 3,640,536 + +0.3 3,640,536 + -3.0 3,640,536 + -0.7 3,640,536 + -2.5 3,640,536 + -2.3 3,640,537 - -1.5 3,640,537 - -0.2 3,640,537 - -1.5 3,640,537 - -2.0 3,640,537 - -1.5 3,640,537 - -0.5 3,640,540 + -1.9 3,640,540 + -2.6 3,640,540 + -0.9 3,640,540 + -0.5 3,640,540 + -3.3 3,640,540 + -3.2 3,640,540 + -2.1 3,640,540 + -2.2 3,640,540 + +1.0 3,640,540 + -2.5 3,640,540 + -2.7 3,640,540 + -3.0 3,640,540 + -2.3 3,640,540 + -1.7 3,640,540 + -0.8 3,640,540 + -3.1 3,640,540 + -1.6 3,640,540 + -4.3 3,640,540 + -1.7 3,640,541 - -1.6 3,640,541 - +0.4 3,640,541 - -0.5 3,640,541 - +0.1 3,640,541 - -1.3 3,640,541 - -1.4 3,640,541 - -1.0 3,640,541 - +0.2 3,640,541 - -1.7 3,640,541 - -0.3 3,640,541 - -1.3 3,640,541 - -0.9 3,640,541 - +0.1 3,640,541 - -0.1 3,640,541 - -0.5 3,640,541 - -0.1 3,640,541 - -0.8 3,640,541 - -2.2 3,640,541 - -0.7 3,640,541 - +0.1 3,640,541 - -3.2 3,640,541 - +0.0 3,640,541 - -0.7 3,640,541 - -2.3 3,640,541 - +1.0 3,640,542 + -1.6 3,640,542 + -2.0 3,640,542 + -1.6 3,640,542 + -1.8 3,640,542 + -3.4 3,640,542 + -1.3 3,640,542 + -2.2 3,640,542 + -1.1 3,640,542 + -1.9 3,640,542 + -2.1 3,640,542 + -0.5 3,640,542 + -1.7 3,640,542 + -2.2 3,640,542 + -1.4 3,640,542 + -3.6 3,640,542 + -4.2 3,640,542 + -2.5 3,640,542 + -2.5 3,640,542 + -2.8 3,640,542 + -2.3 3,640,542 + -1.2 3,640,542 + -3.6 3,640,542 + -1.6 3,640,542 + -2.5 3,640,542 + -0.0 3,640,542 + -3.3 3,640,542 + -2.1 3,640,542 + -1.2 3,640,542 + -2.3 3,640,542 + -1.0 3,640,542 + -1.9 3,640,542 + -1.0 3,640,542 + -2.3 3,640,542 + -2.3 3,640,542 + -2.1 3,640,542 + -1.9 3,640,542 + -3.6 3,640,542 + -2.9 3,640,542 + -1.6 3,640,542 + -2.8 3,640,542 + -2.2 3,640,542 + -3.7 3,640,542 + -2.5 3,640,542 + -0.5 3,640,543 - -1.0 3,640,543 - -0.0 3,640,543 - -0.8 3,640,543 - +0.1 3,640,543 - +0.0 3,640,543 - -1.1 3,640,543 - -0.9 3,640,543 - -1.4 3,640,543 - -0.6 3,640,543 - -1.2 3,640,543 - +0.1 3,640,543 - -0.3 3,640,543 - -0.6 3,640,543 - -0.6 3,640,543 - -0.0 3,640,543 - -1.1 3,640,543 - -0.4 3,640,543 - -0.7 3,640,543 - -0.3 3,640,543 - -0.8 3,640,543 - -1.2 3,640,543 - -1.1 3,640,543 - -0.6 3,640,543 - -0.4 3,640,543 - -0.5 3,640,543 - -1.2 3,640,543 - +0.4 3,640,543 - -1.0 3,640,543 - +1.0 3,640,543 - -0.6 3,640,543 - -0.4 3,640,543 - -1.0 3,640,543 - -0.6 3,640,543 - -1.0 3,640,543 - -0.5 3,640,543 - -0.2 3,640,543 - +0.4 3,640,543 - -0.3 3,640,543 - -0.4 3,640,543 - -0.3 3,640,543 - +1.2 3,640,543 - -0.0 3,640,543 - -0.6 3,640,543 - +1.0 3,640,558 + -2.5 3,640,559 - +0.9 3,640,559 - +0.6 3,640,559 - +1.1 3,640,559 - -0.0 3,640,563 + -1.9 3,640,630 + -1.5 3,640,630 + -0.3 3,640,630 + -0.2 3,640,630 + -0.0 3,640,631 - +0.1 3,640,631 - +0.2 3,640,631 - +1.9 3,640,631 - +0.0 3,640,631 - +0.2 3,640,814 + AO356_16090 0.22 -0.4 3,640,815 - AO356_16090 0.22 +1.5 3,640,904 + AO356_16090 0.32 +0.6 3,640,904 + AO356_16090 0.32 +0.5 3,640,905 - AO356_16090 0.32 +0.1 3,640,905 - AO356_16090 0.32 +1.0 3,640,905 - AO356_16090 0.32 +0.5 3,640,905 - AO356_16090 0.32 +0.0 3,640,997 + AO356_16090 0.42 +1.6 3,640,997 + AO356_16090 0.42 +0.2 3,640,998 - AO356_16090 0.42 +0.7 3,641,003 + AO356_16090 0.43 +0.3 3,641,003 + AO356_16090 0.43 +0.3 3,641,003 + AO356_16090 0.43 +0.3 3,641,003 + AO356_16090 0.43 -0.2 3,641,003 + AO356_16090 0.43 +0.8 3,641,003 + AO356_16090 0.43 -0.2 3,641,003 + AO356_16090 0.43 +0.6 3,641,003 + AO356_16090 0.43 +0.9 3,641,003 + AO356_16090 0.43 -0.0 3,641,003 + AO356_16090 0.43 +0.4 3,641,003 + AO356_16090 0.43 +0.2 3,641,003 + AO356_16090 0.43 -0.2 3,641,004 - AO356_16090 0.43 -0.4 3,641,004 - AO356_16090 0.43 +0.3 3,641,004 - AO356_16090 0.43 +0.2 3,641,004 - AO356_16090 0.43 +1.1 3,641,004 - AO356_16090 0.43 +0.2 3,641,004 - AO356_16090 0.43 +0.4 3,641,004 - AO356_16090 0.43 +0.3 3,641,004 - AO356_16090 0.43 -0.3 3,641,093 + AO356_16090 0.53 +0.2 3,641,093 + AO356_16090 0.53 -2.0 3,641,094 - AO356_16090 0.53 +0.8 3,641,094 - AO356_16090 0.53 -0.3 3,641,180 + AO356_16090 0.63 +0.7 3,641,181 - AO356_16090 0.63 +0.0 3,641,181 - AO356_16090 0.63 +0.3 3,641,181 - AO356_16090 0.63 +0.4 3,641,216 + AO356_16090 0.67 +0.9 3,641,216 + AO356_16090 0.67 -0.3 3,641,217 - AO356_16090 0.67 -0.8 3,641,217 - AO356_16090 0.67 +0.4 3,641,312 + AO356_16090 0.78 -0.0 3,641,313 - AO356_16090 0.78 +0.1 3,641,313 - AO356_16090 0.78 +0.6 3,641,423 + -0.5 3,641,423 + -2.5 3,641,423 + -0.3 3,641,423 + +0.1 3,641,423 + +0.0 3,641,423 + +0.5 3,641,423 + +0.0 3,641,424 - +0.1 3,641,424 - +0.5 3,641,424 - -0.2 3,641,424 - +0.5 3,641,495 + +0.0 3,641,496 - -0.7 3,641,496 - +0.0 3,641,496 - +0.6 3,641,505 + +0.6 3,641,506 - +0.7
Or see this region's nucleotide sequence