Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS36320 and RR42_RS36325 are separated by 179 nucleotides RR42_RS36325 and RR42_RS36330 are separated by 12 nucleotides RR42_RS36330 and RR42_RS36335 are separated by 185 nucleotides
RR42_RS36320: RR42_RS36320 - acetyl-CoA acetyltransferase, at 3,601,252 to 3,602,430
_RS36320
RR42_RS36325: RR42_RS36325 - esterase, at 3,602,610 to 3,603,074
_RS36325
RR42_RS36330: RR42_RS36330 - nitronate monooxygenase, at 3,603,087 to 3,604,061
_RS36330
RR42_RS36335: RR42_RS36335 - enoyl-CoA hydratase, at 3,604,247 to 3,605,134
_RS36335
Position (kb)
3603
3604
3605 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3602.417 kb on + strand at 3602.417 kb on + strand at 3602.418 kb on - strand at 3602.418 kb on - strand at 3602.418 kb on - strand at 3602.418 kb on - strand at 3602.944 kb on + strand, within RR42_RS36325 at 3602.944 kb on + strand, within RR42_RS36325 at 3602.945 kb on - strand, within RR42_RS36325 at 3603.089 kb on - strand at 3603.256 kb on - strand, within RR42_RS36330 at 3603.256 kb on - strand, within RR42_RS36330 at 3603.818 kb on - strand, within RR42_RS36330 at 3603.989 kb on - strand at 3604.165 kb on + strand at 3604.165 kb on + strand at 3604.166 kb on - strand at 3604.166 kb on - strand at 3604.207 kb on + strand at 3604.207 kb on + strand at 3604.500 kb on - strand, within RR42_RS36335 at 3604.778 kb on + strand, within RR42_RS36335
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 3,602,417 + +0.3 3,602,417 + +1.1 3,602,418 - +1.9 3,602,418 - +0.3 3,602,418 - -0.2 3,602,418 - +1.4 3,602,944 + RR42_RS36325 0.72 -0.3 3,602,944 + RR42_RS36325 0.72 +0.6 3,602,945 - RR42_RS36325 0.72 -2.0 3,603,089 - +0.1 3,603,256 - RR42_RS36330 0.17 -1.3 3,603,256 - RR42_RS36330 0.17 -0.8 3,603,818 - RR42_RS36330 0.75 -0.3 3,603,989 - +0.0 3,604,165 + +0.1 3,604,165 + -0.2 3,604,166 - -0.1 3,604,166 - +0.2 3,604,207 + -0.6 3,604,207 + -1.1 3,604,500 - RR42_RS36335 0.28 -0.5 3,604,778 + RR42_RS36335 0.60 -0.6
Or see this region's nucleotide sequence