Experiment: R2A with FW104 filtrate 0.5 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS14585 and LRK54_RS14590 are separated by 30 nucleotides LRK54_RS14590 and LRK54_RS14595 are separated by 90 nucleotides LRK54_RS14595 and LRK54_RS14600 overlap by 4 nucleotides
LRK54_RS14585: LRK54_RS14585 - AraC family transcriptional regulator, at 3,235,309 to 3,236,145
_RS14585
LRK54_RS14590: LRK54_RS14590 - N-acetylmuramoyl-L-alanine amidase, at 3,236,176 to 3,236,730
_RS14590
LRK54_RS14595: LRK54_RS14595 - alpha/beta hydrolase, at 3,236,821 to 3,237,348
_RS14595
LRK54_RS14600: LRK54_RS14600 - bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL, at 3,237,345 to 3,239,531
_RS14600
Position (kb)
3236
3237
3238 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3235.928 kb on + strand, within LRK54_RS14585 at 3235.928 kb on + strand, within LRK54_RS14585 at 3235.929 kb on - strand, within LRK54_RS14585 at 3235.929 kb on - strand, within LRK54_RS14585 at 3236.177 kb on + strand at 3236.177 kb on + strand at 3236.177 kb on + strand at 3236.178 kb on - strand at 3236.178 kb on - strand at 3236.178 kb on - strand at 3236.394 kb on - strand, within LRK54_RS14590 at 3236.522 kb on + strand, within LRK54_RS14590 at 3236.522 kb on + strand, within LRK54_RS14590 at 3236.522 kb on + strand, within LRK54_RS14590 at 3236.522 kb on + strand, within LRK54_RS14590 at 3236.522 kb on + strand, within LRK54_RS14590 at 3236.523 kb on - strand, within LRK54_RS14590 at 3236.523 kb on - strand, within LRK54_RS14590 at 3236.523 kb on - strand, within LRK54_RS14590 at 3236.523 kb on - strand, within LRK54_RS14590 at 3236.528 kb on + strand, within LRK54_RS14590 at 3236.528 kb on + strand, within LRK54_RS14590 at 3236.528 kb on + strand, within LRK54_RS14590 at 3236.528 kb on + strand, within LRK54_RS14590 at 3236.528 kb on + strand, within LRK54_RS14590 at 3236.528 kb on + strand, within LRK54_RS14590 at 3236.528 kb on + strand, within LRK54_RS14590 at 3236.529 kb on - strand, within LRK54_RS14590 at 3236.529 kb on - strand, within LRK54_RS14590 at 3236.529 kb on - strand, within LRK54_RS14590 at 3236.529 kb on - strand, within LRK54_RS14590 at 3236.529 kb on - strand, within LRK54_RS14590 at 3236.529 kb on - strand, within LRK54_RS14590 at 3236.529 kb on - strand, within LRK54_RS14590 at 3236.529 kb on - strand, within LRK54_RS14590 at 3236.552 kb on + strand, within LRK54_RS14590 at 3236.552 kb on + strand, within LRK54_RS14590 at 3236.552 kb on + strand, within LRK54_RS14590 at 3236.552 kb on + strand, within LRK54_RS14590 at 3236.552 kb on + strand, within LRK54_RS14590 at 3236.552 kb on + strand, within LRK54_RS14590 at 3236.552 kb on + strand, within LRK54_RS14590 at 3236.553 kb on - strand, within LRK54_RS14590 at 3236.553 kb on - strand, within LRK54_RS14590 at 3236.553 kb on - strand, within LRK54_RS14590 at 3236.553 kb on - strand, within LRK54_RS14590 at 3236.553 kb on - strand, within LRK54_RS14590 at 3236.553 kb on - strand, within LRK54_RS14590 at 3236.553 kb on - strand, within LRK54_RS14590 at 3236.553 kb on - strand, within LRK54_RS14590 at 3236.553 kb on - strand, within LRK54_RS14590 at 3236.553 kb on - strand, within LRK54_RS14590 at 3236.553 kb on - strand, within LRK54_RS14590 at 3236.553 kb on - strand, within LRK54_RS14590 at 3236.553 kb on - strand, within LRK54_RS14590 at 3236.555 kb on + strand, within LRK54_RS14590 at 3236.555 kb on + strand, within LRK54_RS14590 at 3236.555 kb on + strand, within LRK54_RS14590 at 3236.555 kb on + strand, within LRK54_RS14590 at 3236.555 kb on + strand, within LRK54_RS14590 at 3236.555 kb on + strand, within LRK54_RS14590 at 3236.556 kb on - strand, within LRK54_RS14590 at 3236.556 kb on - strand, within LRK54_RS14590 at 3236.556 kb on - strand, within LRK54_RS14590 at 3236.556 kb on - strand, within LRK54_RS14590 at 3236.556 kb on - strand, within LRK54_RS14590 at 3236.556 kb on - strand, within LRK54_RS14590 at 3236.556 kb on - strand, within LRK54_RS14590 at 3236.556 kb on - strand, within LRK54_RS14590 at 3236.603 kb on + strand, within LRK54_RS14590 at 3236.696 kb on + strand at 3236.696 kb on + strand at 3236.696 kb on + strand at 3236.696 kb on + strand at 3236.696 kb on + strand at 3236.696 kb on + strand at 3236.696 kb on + strand at 3236.696 kb on + strand at 3236.696 kb on + strand at 3236.696 kb on + strand at 3236.697 kb on - strand at 3236.697 kb on - strand at 3236.697 kb on - strand at 3236.697 kb on - strand at 3236.697 kb on - strand at 3236.697 kb on - strand at 3236.697 kb on - strand at 3236.697 kb on - strand at 3236.697 kb on - strand at 3236.697 kb on - strand at 3236.697 kb on - strand at 3236.697 kb on - strand at 3236.697 kb on - strand at 3236.778 kb on + strand at 3236.778 kb on + strand at 3236.778 kb on + strand at 3236.778 kb on + strand at 3236.778 kb on + strand at 3236.779 kb on - strand at 3236.919 kb on + strand, within LRK54_RS14595 at 3236.919 kb on + strand, within LRK54_RS14595 at 3236.919 kb on + strand, within LRK54_RS14595 at 3236.919 kb on + strand, within LRK54_RS14595 at 3236.919 kb on + strand, within LRK54_RS14595 at 3236.919 kb on + strand, within LRK54_RS14595 at 3236.920 kb on - strand, within LRK54_RS14595 at 3236.920 kb on - strand, within LRK54_RS14595 at 3236.920 kb on - strand, within LRK54_RS14595 at 3236.920 kb on - strand, within LRK54_RS14595 at 3237.133 kb on + strand, within LRK54_RS14595 at 3237.134 kb on - strand, within LRK54_RS14595 at 3237.134 kb on - strand, within LRK54_RS14595 at 3237.354 kb on + strand at 3237.354 kb on + strand at 3237.354 kb on + strand at 3237.354 kb on + strand at 3237.355 kb on - strand at 3237.355 kb on - strand at 3237.355 kb on - strand at 3237.355 kb on - strand at 3237.362 kb on + strand at 3237.384 kb on + strand at 3237.384 kb on + strand at 3237.384 kb on + strand at 3237.384 kb on + strand at 3237.384 kb on + strand at 3237.384 kb on + strand at 3237.385 kb on - strand at 3237.385 kb on - strand at 3237.385 kb on - strand at 3237.385 kb on - strand at 3237.385 kb on - strand at 3237.385 kb on - strand at 3237.385 kb on - strand at 3237.477 kb on + strand at 3237.477 kb on + strand at 3237.477 kb on + strand at 3237.477 kb on + strand at 3237.477 kb on + strand at 3237.477 kb on + strand at 3237.477 kb on + strand at 3237.477 kb on + strand at 3237.478 kb on - strand at 3237.478 kb on - strand at 3237.478 kb on - strand at 3237.478 kb on - strand at 3237.478 kb on - strand at 3237.478 kb on - strand at 3237.561 kb on + strand at 3237.561 kb on + strand at 3237.561 kb on + strand at 3237.561 kb on + strand at 3237.561 kb on + strand at 3237.561 kb on + strand at 3237.562 kb on - strand at 3237.562 kb on - strand at 3237.562 kb on - strand at 3237.562 kb on - strand at 3237.562 kb on - strand at 3237.562 kb on - strand at 3237.562 kb on - strand at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.913 kb on + strand, within LRK54_RS14600 at 3237.914 kb on - strand, within LRK54_RS14600 at 3237.914 kb on - strand, within LRK54_RS14600 at 3237.914 kb on - strand, within LRK54_RS14600 at 3237.914 kb on - strand, within LRK54_RS14600 at 3237.914 kb on - strand, within LRK54_RS14600 at 3237.914 kb on - strand, within LRK54_RS14600 at 3237.914 kb on - strand, within LRK54_RS14600 at 3237.914 kb on - strand, within LRK54_RS14600 at 3237.914 kb on - strand, within LRK54_RS14600 at 3237.914 kb on - strand, within LRK54_RS14600 at 3237.915 kb on + strand, within LRK54_RS14600 at 3237.915 kb on + strand, within LRK54_RS14600 at 3237.915 kb on + strand, within LRK54_RS14600 at 3237.915 kb on + strand, within LRK54_RS14600 at 3237.915 kb on + strand, within LRK54_RS14600 at 3237.915 kb on + strand, within LRK54_RS14600 at 3237.915 kb on + strand, within LRK54_RS14600 at 3237.915 kb on + strand, within LRK54_RS14600 at 3237.915 kb on + strand, within LRK54_RS14600 at 3237.915 kb on + strand, within LRK54_RS14600 at 3237.915 kb on + strand, within LRK54_RS14600 at 3237.916 kb on - strand, within LRK54_RS14600 at 3237.916 kb on - strand, within LRK54_RS14600 at 3237.916 kb on - strand, within LRK54_RS14600 at 3237.916 kb on - strand, within LRK54_RS14600 at 3237.916 kb on - strand, within LRK54_RS14600 at 3237.916 kb on - strand, within LRK54_RS14600 at 3237.916 kb on - strand, within LRK54_RS14600 at 3237.916 kb on - strand, within LRK54_RS14600 at 3237.916 kb on - strand, within LRK54_RS14600 at 3237.916 kb on - strand, within LRK54_RS14600 at 3237.916 kb on - strand, within LRK54_RS14600 at 3237.916 kb on - strand, within LRK54_RS14600 at 3237.916 kb on - strand, within LRK54_RS14600 at 3237.916 kb on - strand, within LRK54_RS14600 at 3237.916 kb on - strand, within LRK54_RS14600 at 3237.916 kb on - strand, within LRK54_RS14600 at 3238.020 kb on + strand, within LRK54_RS14600 at 3238.020 kb on + strand, within LRK54_RS14600 at 3238.020 kb on + strand, within LRK54_RS14600 at 3238.021 kb on - strand, within LRK54_RS14600 at 3238.021 kb on - strand, within LRK54_RS14600 at 3238.329 kb on + strand, within LRK54_RS14600 at 3238.330 kb on - strand, within LRK54_RS14600
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with FW104 filtrate 0.5 X remove 3,235,928 + LRK54_RS14585 0.74 -0.8 3,235,928 + LRK54_RS14585 0.74 +0.7 3,235,929 - LRK54_RS14585 0.74 +1.3 3,235,929 - LRK54_RS14585 0.74 -0.2 3,236,177 + -0.1 3,236,177 + +0.1 3,236,177 + -0.6 3,236,178 - +1.3 3,236,178 - -0.1 3,236,178 - -0.9 3,236,394 - LRK54_RS14590 0.39 -0.6 3,236,522 + LRK54_RS14590 0.62 -1.9 3,236,522 + LRK54_RS14590 0.62 -1.2 3,236,522 + LRK54_RS14590 0.62 -1.2 3,236,522 + LRK54_RS14590 0.62 -1.1 3,236,522 + LRK54_RS14590 0.62 +0.7 3,236,523 - LRK54_RS14590 0.63 -2.3 3,236,523 - LRK54_RS14590 0.63 -2.9 3,236,523 - LRK54_RS14590 0.63 -3.0 3,236,523 - LRK54_RS14590 0.63 -0.1 3,236,528 + LRK54_RS14590 0.63 -1.7 3,236,528 + LRK54_RS14590 0.63 -1.7 3,236,528 + LRK54_RS14590 0.63 -0.1 3,236,528 + LRK54_RS14590 0.63 -0.7 3,236,528 + LRK54_RS14590 0.63 -0.3 3,236,528 + LRK54_RS14590 0.63 +0.2 3,236,528 + LRK54_RS14590 0.63 -0.1 3,236,529 - LRK54_RS14590 0.64 +0.3 3,236,529 - LRK54_RS14590 0.64 -1.4 3,236,529 - LRK54_RS14590 0.64 -2.0 3,236,529 - LRK54_RS14590 0.64 +0.3 3,236,529 - LRK54_RS14590 0.64 -0.7 3,236,529 - LRK54_RS14590 0.64 -0.3 3,236,529 - LRK54_RS14590 0.64 -1.1 3,236,529 - LRK54_RS14590 0.64 -1.6 3,236,552 + LRK54_RS14590 0.68 -1.4 3,236,552 + LRK54_RS14590 0.68 +0.3 3,236,552 + LRK54_RS14590 0.68 -2.0 3,236,552 + LRK54_RS14590 0.68 -0.1 3,236,552 + LRK54_RS14590 0.68 -0.6 3,236,552 + LRK54_RS14590 0.68 -1.4 3,236,552 + LRK54_RS14590 0.68 +0.7 3,236,553 - LRK54_RS14590 0.68 -0.7 3,236,553 - LRK54_RS14590 0.68 -0.1 3,236,553 - LRK54_RS14590 0.68 -1.1 3,236,553 - LRK54_RS14590 0.68 -0.9 3,236,553 - LRK54_RS14590 0.68 -0.1 3,236,553 - LRK54_RS14590 0.68 -2.7 3,236,553 - LRK54_RS14590 0.68 -1.1 3,236,553 - LRK54_RS14590 0.68 -1.7 3,236,553 - LRK54_RS14590 0.68 -2.3 3,236,553 - LRK54_RS14590 0.68 -1.8 3,236,553 - LRK54_RS14590 0.68 -0.1 3,236,553 - LRK54_RS14590 0.68 -2.2 3,236,553 - LRK54_RS14590 0.68 -0.7 3,236,555 + LRK54_RS14590 0.68 +0.3 3,236,555 + LRK54_RS14590 0.68 +0.3 3,236,555 + LRK54_RS14590 0.68 -1.9 3,236,555 + LRK54_RS14590 0.68 -2.1 3,236,555 + LRK54_RS14590 0.68 -0.3 3,236,555 + LRK54_RS14590 0.68 -1.1 3,236,556 - LRK54_RS14590 0.68 -0.6 3,236,556 - LRK54_RS14590 0.68 -1.6 3,236,556 - LRK54_RS14590 0.68 -0.3 3,236,556 - LRK54_RS14590 0.68 +0.4 3,236,556 - LRK54_RS14590 0.68 -1.2 3,236,556 - LRK54_RS14590 0.68 -0.7 3,236,556 - LRK54_RS14590 0.68 -2.3 3,236,556 - LRK54_RS14590 0.68 -0.7 3,236,603 + LRK54_RS14590 0.77 -0.6 3,236,696 + -2.2 3,236,696 + -1.1 3,236,696 + -0.6 3,236,696 + +0.7 3,236,696 + -1.2 3,236,696 + -0.9 3,236,696 + -1.2 3,236,696 + +0.3 3,236,696 + -0.9 3,236,696 + -0.3 3,236,697 - -1.7 3,236,697 - -3.8 3,236,697 - -1.6 3,236,697 - -0.3 3,236,697 - -1.4 3,236,697 - -0.1 3,236,697 - -1.7 3,236,697 - -1.2 3,236,697 - -1.7 3,236,697 - -0.7 3,236,697 - -1.3 3,236,697 - +1.3 3,236,697 - -0.3 3,236,778 + -0.2 3,236,778 + +0.6 3,236,778 + +1.7 3,236,778 + +0.7 3,236,778 + +0.2 3,236,779 - -1.0 3,236,919 + LRK54_RS14595 0.19 -0.1 3,236,919 + LRK54_RS14595 0.19 +0.1 3,236,919 + LRK54_RS14595 0.19 -0.0 3,236,919 + LRK54_RS14595 0.19 +0.2 3,236,919 + LRK54_RS14595 0.19 +2.1 3,236,919 + LRK54_RS14595 0.19 -0.3 3,236,920 - LRK54_RS14595 0.19 +0.1 3,236,920 - LRK54_RS14595 0.19 -0.0 3,236,920 - LRK54_RS14595 0.19 +1.4 3,236,920 - LRK54_RS14595 0.19 -0.1 3,237,133 + LRK54_RS14595 0.59 +0.6 3,237,134 - LRK54_RS14595 0.59 -0.6 3,237,134 - LRK54_RS14595 0.59 +0.4 3,237,354 + +0.7 3,237,354 + +0.3 3,237,354 + -0.2 3,237,354 + +1.0 3,237,355 - -0.2 3,237,355 - +2.0 3,237,355 - -0.6 3,237,355 - +1.7 3,237,362 + -0.7 3,237,384 + +0.3 3,237,384 + -0.6 3,237,384 + -1.1 3,237,384 + -0.6 3,237,384 + +0.3 3,237,384 + -0.3 3,237,385 - +0.1 3,237,385 - -0.2 3,237,385 - -0.3 3,237,385 - -0.8 3,237,385 - +0.3 3,237,385 - -1.6 3,237,385 - -0.9 3,237,477 + -0.8 3,237,477 + -0.2 3,237,477 + +1.0 3,237,477 + +1.6 3,237,477 + +0.1 3,237,477 + -0.3 3,237,477 + +1.7 3,237,477 + -1.6 3,237,478 - +0.3 3,237,478 - -0.6 3,237,478 - +0.9 3,237,478 - +0.2 3,237,478 - +0.2 3,237,478 - -0.1 3,237,561 + +0.6 3,237,561 + -1.1 3,237,561 + -0.2 3,237,561 + -0.3 3,237,561 + -1.1 3,237,561 + +0.1 3,237,562 - -1.2 3,237,562 - -0.2 3,237,562 - -0.6 3,237,562 - +1.1 3,237,562 - +1.2 3,237,562 - -0.8 3,237,562 - +0.4 3,237,913 + LRK54_RS14600 0.26 -0.3 3,237,913 + LRK54_RS14600 0.26 +0.6 3,237,913 + LRK54_RS14600 0.26 -1.4 3,237,913 + LRK54_RS14600 0.26 +0.0 3,237,913 + LRK54_RS14600 0.26 -1.4 3,237,913 + LRK54_RS14600 0.26 +0.8 3,237,913 + LRK54_RS14600 0.26 -1.2 3,237,913 + LRK54_RS14600 0.26 -0.9 3,237,913 + LRK54_RS14600 0.26 -0.5 3,237,913 + LRK54_RS14600 0.26 -1.0 3,237,913 + LRK54_RS14600 0.26 -0.5 3,237,913 + LRK54_RS14600 0.26 -0.0 3,237,913 + LRK54_RS14600 0.26 -0.7 3,237,913 + LRK54_RS14600 0.26 +0.7 3,237,913 + LRK54_RS14600 0.26 +1.3 3,237,913 + LRK54_RS14600 0.26 +0.9 3,237,913 + LRK54_RS14600 0.26 +1.3 3,237,913 + LRK54_RS14600 0.26 +0.1 3,237,913 + LRK54_RS14600 0.26 +0.4 3,237,913 + LRK54_RS14600 0.26 +0.8 3,237,914 - LRK54_RS14600 0.26 -0.2 3,237,914 - LRK54_RS14600 0.26 +0.1 3,237,914 - LRK54_RS14600 0.26 +2.3 3,237,914 - LRK54_RS14600 0.26 -0.8 3,237,914 - LRK54_RS14600 0.26 +1.4 3,237,914 - LRK54_RS14600 0.26 +0.3 3,237,914 - LRK54_RS14600 0.26 -0.3 3,237,914 - LRK54_RS14600 0.26 -0.6 3,237,914 - LRK54_RS14600 0.26 +0.1 3,237,914 - LRK54_RS14600 0.26 -0.6 3,237,915 + LRK54_RS14600 0.26 +0.1 3,237,915 + LRK54_RS14600 0.26 +0.2 3,237,915 + LRK54_RS14600 0.26 -0.9 3,237,915 + LRK54_RS14600 0.26 -0.6 3,237,915 + LRK54_RS14600 0.26 +0.4 3,237,915 + LRK54_RS14600 0.26 -0.9 3,237,915 + LRK54_RS14600 0.26 -0.9 3,237,915 + LRK54_RS14600 0.26 +0.1 3,237,915 + LRK54_RS14600 0.26 -0.8 3,237,915 + LRK54_RS14600 0.26 -0.1 3,237,915 + LRK54_RS14600 0.26 +0.6 3,237,916 - LRK54_RS14600 0.26 +0.4 3,237,916 - LRK54_RS14600 0.26 +0.9 3,237,916 - LRK54_RS14600 0.26 +0.3 3,237,916 - LRK54_RS14600 0.26 -0.5 3,237,916 - LRK54_RS14600 0.26 -0.3 3,237,916 - LRK54_RS14600 0.26 -1.8 3,237,916 - LRK54_RS14600 0.26 +0.2 3,237,916 - LRK54_RS14600 0.26 +0.3 3,237,916 - LRK54_RS14600 0.26 +0.5 3,237,916 - LRK54_RS14600 0.26 +0.6 3,237,916 - LRK54_RS14600 0.26 +0.4 3,237,916 - LRK54_RS14600 0.26 -0.2 3,237,916 - LRK54_RS14600 0.26 +0.1 3,237,916 - LRK54_RS14600 0.26 +0.4 3,237,916 - LRK54_RS14600 0.26 +0.8 3,237,916 - LRK54_RS14600 0.26 -0.7 3,238,020 + LRK54_RS14600 0.31 -0.5 3,238,020 + LRK54_RS14600 0.31 +0.6 3,238,020 + LRK54_RS14600 0.31 -0.6 3,238,021 - LRK54_RS14600 0.31 -0.1 3,238,021 - LRK54_RS14600 0.31 +0.4 3,238,329 + LRK54_RS14600 0.45 -0.4 3,238,330 - LRK54_RS14600 0.45 -0.7
Or see this region's nucleotide sequence