Experiment: R2A with FW104 filtrate 0.5 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS14515 and LRK54_RS14520 are separated by 83 nucleotides LRK54_RS14520 and LRK54_RS14525 are separated by 106 nucleotides LRK54_RS14525 and LRK54_RS14530 overlap by 4 nucleotides LRK54_RS14530 and LRK54_RS14535 are separated by 26 nucleotides LRK54_RS14535 and LRK54_RS14540 are separated by 9 nucleotides
LRK54_RS14515: LRK54_RS14515 - SCO family protein, at 3,224,364 to 3,224,981
_RS14515
LRK54_RS14520: LRK54_RS14520 - DUF423 domain-containing protein, at 3,225,065 to 3,225,442
_RS14520
LRK54_RS14525: LRK54_RS14525 - hypothetical protein, at 3,225,549 to 3,225,905
_RS14525
LRK54_RS14530: LRK54_RS14530 - CPBP family glutamic-type intramembrane protease, at 3,225,902 to 3,226,672
_RS14530
LRK54_RS14535: LRK54_RS14535 - hypothetical protein, at 3,226,699 to 3,226,893
_RS14535
LRK54_RS14540: LRK54_RS14540 - SPFH domain-containing protein, at 3,226,903 to 3,227,778
_RS14540
Position (kb)
3225
3226 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3224.565 kb on + strand, within LRK54_RS14515 at 3224.774 kb on - strand, within LRK54_RS14515 at 3224.784 kb on + strand, within LRK54_RS14515 at 3225.014 kb on + strand at 3225.014 kb on + strand at 3225.014 kb on + strand at 3225.014 kb on + strand at 3225.014 kb on + strand at 3225.014 kb on + strand at 3225.014 kb on + strand at 3225.014 kb on + strand at 3225.014 kb on + strand at 3225.015 kb on - strand at 3225.015 kb on - strand at 3225.015 kb on - strand at 3225.015 kb on - strand at 3225.015 kb on - strand at 3225.015 kb on - strand at 3225.015 kb on - strand at 3225.015 kb on - strand at 3225.015 kb on - strand at 3225.015 kb on - strand at 3225.015 kb on - strand at 3225.015 kb on - strand at 3225.015 kb on - strand at 3225.015 kb on - strand at 3225.015 kb on - strand at 3225.015 kb on - strand at 3225.015 kb on - strand at 3225.083 kb on - strand at 3225.196 kb on + strand, within LRK54_RS14520 at 3225.196 kb on + strand, within LRK54_RS14520 at 3225.196 kb on + strand, within LRK54_RS14520 at 3225.196 kb on + strand, within LRK54_RS14520 at 3225.197 kb on - strand, within LRK54_RS14520 at 3225.197 kb on - strand, within LRK54_RS14520 at 3225.197 kb on - strand, within LRK54_RS14520 at 3225.197 kb on - strand, within LRK54_RS14520 at 3225.209 kb on + strand, within LRK54_RS14520 at 3225.209 kb on + strand, within LRK54_RS14520 at 3225.209 kb on + strand, within LRK54_RS14520 at 3225.209 kb on + strand, within LRK54_RS14520 at 3225.209 kb on + strand, within LRK54_RS14520 at 3225.209 kb on + strand, within LRK54_RS14520 at 3225.209 kb on + strand, within LRK54_RS14520 at 3225.209 kb on + strand, within LRK54_RS14520 at 3225.209 kb on + strand, within LRK54_RS14520 at 3225.209 kb on + strand, within LRK54_RS14520 at 3225.209 kb on + strand, within LRK54_RS14520 at 3225.210 kb on - strand, within LRK54_RS14520 at 3225.210 kb on - strand, within LRK54_RS14520 at 3225.210 kb on - strand, within LRK54_RS14520 at 3225.210 kb on - strand, within LRK54_RS14520 at 3225.210 kb on - strand, within LRK54_RS14520 at 3225.210 kb on - strand, within LRK54_RS14520 at 3225.210 kb on - strand, within LRK54_RS14520 at 3225.210 kb on - strand, within LRK54_RS14520 at 3225.210 kb on - strand, within LRK54_RS14520 at 3225.210 kb on - strand, within LRK54_RS14520 at 3225.326 kb on + strand, within LRK54_RS14520 at 3225.326 kb on + strand, within LRK54_RS14520 at 3225.326 kb on + strand, within LRK54_RS14520 at 3225.326 kb on + strand, within LRK54_RS14520 at 3225.326 kb on + strand, within LRK54_RS14520 at 3225.326 kb on + strand, within LRK54_RS14520 at 3225.326 kb on + strand, within LRK54_RS14520 at 3225.327 kb on - strand, within LRK54_RS14520 at 3225.327 kb on - strand, within LRK54_RS14520 at 3225.327 kb on - strand, within LRK54_RS14520 at 3225.327 kb on - strand, within LRK54_RS14520 at 3225.434 kb on - strand at 3225.440 kb on + strand at 3225.440 kb on + strand at 3225.440 kb on + strand at 3225.440 kb on + strand at 3225.440 kb on + strand at 3225.440 kb on + strand at 3225.440 kb on + strand at 3225.440 kb on + strand at 3225.440 kb on + strand at 3225.440 kb on + strand at 3225.440 kb on + strand at 3225.441 kb on - strand at 3225.441 kb on - strand at 3225.441 kb on - strand at 3225.441 kb on - strand at 3225.441 kb on - strand at 3225.441 kb on - strand at 3225.441 kb on - strand at 3225.732 kb on + strand, within LRK54_RS14525 at 3225.732 kb on + strand, within LRK54_RS14525 at 3225.732 kb on + strand, within LRK54_RS14525 at 3225.732 kb on + strand, within LRK54_RS14525 at 3225.732 kb on + strand, within LRK54_RS14525 at 3225.732 kb on + strand, within LRK54_RS14525 at 3225.733 kb on - strand, within LRK54_RS14525 at 3225.733 kb on - strand, within LRK54_RS14525 at 3225.733 kb on - strand, within LRK54_RS14525 at 3225.733 kb on - strand, within LRK54_RS14525 at 3225.733 kb on - strand, within LRK54_RS14525 at 3225.733 kb on - strand, within LRK54_RS14525 at 3225.903 kb on + strand at 3225.984 kb on + strand, within LRK54_RS14530 at 3225.984 kb on + strand, within LRK54_RS14530 at 3225.985 kb on - strand, within LRK54_RS14530 at 3225.985 kb on - strand, within LRK54_RS14530 at 3225.985 kb on - strand, within LRK54_RS14530 at 3225.985 kb on - strand, within LRK54_RS14530 at 3225.985 kb on - strand, within LRK54_RS14530 at 3225.985 kb on - strand, within LRK54_RS14530 at 3225.985 kb on - strand, within LRK54_RS14530 at 3225.985 kb on - strand, within LRK54_RS14530 at 3225.985 kb on - strand, within LRK54_RS14530 at 3225.985 kb on - strand, within LRK54_RS14530 at 3225.985 kb on - strand, within LRK54_RS14530 at 3226.242 kb on + strand, within LRK54_RS14530 at 3226.242 kb on + strand, within LRK54_RS14530 at 3226.242 kb on + strand, within LRK54_RS14530 at 3226.243 kb on - strand, within LRK54_RS14530 at 3226.243 kb on - strand, within LRK54_RS14530 at 3226.243 kb on - strand, within LRK54_RS14530 at 3226.243 kb on - strand, within LRK54_RS14530 at 3226.435 kb on - strand, within LRK54_RS14530 at 3226.449 kb on + strand, within LRK54_RS14530 at 3226.449 kb on + strand, within LRK54_RS14530 at 3226.450 kb on - strand, within LRK54_RS14530 at 3226.450 kb on - strand, within LRK54_RS14530 at 3226.450 kb on - strand, within LRK54_RS14530 at 3226.450 kb on - strand, within LRK54_RS14530 at 3226.450 kb on - strand, within LRK54_RS14530 at 3226.450 kb on - strand, within LRK54_RS14530 at 3226.450 kb on - strand, within LRK54_RS14530 at 3226.450 kb on - strand, within LRK54_RS14530 at 3226.450 kb on - strand, within LRK54_RS14530 at 3226.569 kb on + strand, within LRK54_RS14530 at 3226.784 kb on + strand, within LRK54_RS14535 at 3226.784 kb on + strand, within LRK54_RS14535 at 3226.784 kb on + strand, within LRK54_RS14535 at 3226.784 kb on + strand, within LRK54_RS14535 at 3226.784 kb on + strand, within LRK54_RS14535 at 3226.784 kb on + strand, within LRK54_RS14535 at 3226.784 kb on + strand, within LRK54_RS14535 at 3226.784 kb on + strand, within LRK54_RS14535 at 3226.784 kb on + strand, within LRK54_RS14535 at 3226.785 kb on - strand, within LRK54_RS14535 at 3226.785 kb on - strand, within LRK54_RS14535 at 3226.785 kb on - strand, within LRK54_RS14535 at 3226.785 kb on - strand, within LRK54_RS14535 at 3226.785 kb on - strand, within LRK54_RS14535 at 3226.785 kb on - strand, within LRK54_RS14535 at 3226.785 kb on - strand, within LRK54_RS14535 at 3226.785 kb on - strand, within LRK54_RS14535 at 3226.790 kb on - strand, within LRK54_RS14535 at 3226.872 kb on - strand, within LRK54_RS14535
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with FW104 filtrate 0.5 X remove 3,224,565 + LRK54_RS14515 0.33 -1.2 3,224,774 - LRK54_RS14515 0.66 -1.2 3,224,784 + LRK54_RS14515 0.68 -0.1 3,225,014 + +0.7 3,225,014 + +0.7 3,225,014 + +1.5 3,225,014 + -0.3 3,225,014 + +1.5 3,225,014 + -0.7 3,225,014 + -1.1 3,225,014 + -0.4 3,225,014 + -0.1 3,225,015 - -0.6 3,225,015 - +0.2 3,225,015 - +0.2 3,225,015 - -0.0 3,225,015 - +0.0 3,225,015 - +1.0 3,225,015 - -0.1 3,225,015 - -0.7 3,225,015 - -1.5 3,225,015 - -0.2 3,225,015 - -0.2 3,225,015 - -0.3 3,225,015 - +1.2 3,225,015 - -0.2 3,225,015 - -3.2 3,225,015 - +0.2 3,225,015 - -0.3 3,225,083 - -1.0 3,225,196 + LRK54_RS14520 0.35 -4.0 3,225,196 + LRK54_RS14520 0.35 -0.0 3,225,196 + LRK54_RS14520 0.35 -0.3 3,225,196 + LRK54_RS14520 0.35 +1.1 3,225,197 - LRK54_RS14520 0.35 +0.4 3,225,197 - LRK54_RS14520 0.35 -0.9 3,225,197 - LRK54_RS14520 0.35 -0.7 3,225,197 - LRK54_RS14520 0.35 -0.1 3,225,209 + LRK54_RS14520 0.38 -2.1 3,225,209 + LRK54_RS14520 0.38 +1.2 3,225,209 + LRK54_RS14520 0.38 -0.0 3,225,209 + LRK54_RS14520 0.38 -1.2 3,225,209 + LRK54_RS14520 0.38 +1.0 3,225,209 + LRK54_RS14520 0.38 +0.4 3,225,209 + LRK54_RS14520 0.38 -0.4 3,225,209 + LRK54_RS14520 0.38 -0.8 3,225,209 + LRK54_RS14520 0.38 +0.4 3,225,209 + LRK54_RS14520 0.38 -0.3 3,225,209 + LRK54_RS14520 0.38 +0.5 3,225,210 - LRK54_RS14520 0.38 +0.0 3,225,210 - LRK54_RS14520 0.38 -0.7 3,225,210 - LRK54_RS14520 0.38 +1.1 3,225,210 - LRK54_RS14520 0.38 -0.1 3,225,210 - LRK54_RS14520 0.38 -2.8 3,225,210 - LRK54_RS14520 0.38 +0.1 3,225,210 - LRK54_RS14520 0.38 -0.5 3,225,210 - LRK54_RS14520 0.38 +0.2 3,225,210 - LRK54_RS14520 0.38 +0.1 3,225,210 - LRK54_RS14520 0.38 +0.1 3,225,326 + LRK54_RS14520 0.69 +0.4 3,225,326 + LRK54_RS14520 0.69 -0.8 3,225,326 + LRK54_RS14520 0.69 +0.1 3,225,326 + LRK54_RS14520 0.69 -0.2 3,225,326 + LRK54_RS14520 0.69 +0.7 3,225,326 + LRK54_RS14520 0.69 +0.0 3,225,326 + LRK54_RS14520 0.69 -1.1 3,225,327 - LRK54_RS14520 0.69 +3.8 3,225,327 - LRK54_RS14520 0.69 +0.0 3,225,327 - LRK54_RS14520 0.69 -0.2 3,225,327 - LRK54_RS14520 0.69 -0.1 3,225,434 - -0.8 3,225,440 + -0.3 3,225,440 + -1.7 3,225,440 + +1.0 3,225,440 + +0.4 3,225,440 + -1.5 3,225,440 + +0.1 3,225,440 + -0.8 3,225,440 + -0.3 3,225,440 + -1.6 3,225,440 + -0.6 3,225,440 + -1.1 3,225,441 - -0.1 3,225,441 - -0.8 3,225,441 - -0.9 3,225,441 - -0.6 3,225,441 - +0.1 3,225,441 - -2.1 3,225,441 - -0.9 3,225,732 + LRK54_RS14525 0.51 -0.5 3,225,732 + LRK54_RS14525 0.51 +0.5 3,225,732 + LRK54_RS14525 0.51 +0.6 3,225,732 + LRK54_RS14525 0.51 -0.5 3,225,732 + LRK54_RS14525 0.51 +0.1 3,225,732 + LRK54_RS14525 0.51 +0.2 3,225,733 - LRK54_RS14525 0.52 -0.6 3,225,733 - LRK54_RS14525 0.52 +0.2 3,225,733 - LRK54_RS14525 0.52 +0.4 3,225,733 - LRK54_RS14525 0.52 +0.8 3,225,733 - LRK54_RS14525 0.52 +1.3 3,225,733 - LRK54_RS14525 0.52 -1.6 3,225,903 + +0.3 3,225,984 + LRK54_RS14530 0.11 -0.3 3,225,984 + LRK54_RS14530 0.11 +0.5 3,225,985 - LRK54_RS14530 0.11 +0.6 3,225,985 - LRK54_RS14530 0.11 -1.6 3,225,985 - LRK54_RS14530 0.11 -0.3 3,225,985 - LRK54_RS14530 0.11 -0.3 3,225,985 - LRK54_RS14530 0.11 +0.3 3,225,985 - LRK54_RS14530 0.11 +0.4 3,225,985 - LRK54_RS14530 0.11 +0.8 3,225,985 - LRK54_RS14530 0.11 -0.9 3,225,985 - LRK54_RS14530 0.11 +2.3 3,225,985 - LRK54_RS14530 0.11 +0.4 3,225,985 - LRK54_RS14530 0.11 -1.2 3,226,242 + LRK54_RS14530 0.44 -0.1 3,226,242 + LRK54_RS14530 0.44 -0.1 3,226,242 + LRK54_RS14530 0.44 +0.1 3,226,243 - LRK54_RS14530 0.44 -1.6 3,226,243 - LRK54_RS14530 0.44 +0.1 3,226,243 - LRK54_RS14530 0.44 -0.9 3,226,243 - LRK54_RS14530 0.44 +0.3 3,226,435 - LRK54_RS14530 0.69 -0.6 3,226,449 + LRK54_RS14530 0.71 -0.8 3,226,449 + LRK54_RS14530 0.71 -0.6 3,226,450 - LRK54_RS14530 0.71 -0.2 3,226,450 - LRK54_RS14530 0.71 +0.3 3,226,450 - LRK54_RS14530 0.71 -0.4 3,226,450 - LRK54_RS14530 0.71 +0.8 3,226,450 - LRK54_RS14530 0.71 -0.8 3,226,450 - LRK54_RS14530 0.71 +0.3 3,226,450 - LRK54_RS14530 0.71 -0.5 3,226,450 - LRK54_RS14530 0.71 +1.4 3,226,450 - LRK54_RS14530 0.71 +0.6 3,226,569 + LRK54_RS14530 0.87 +0.5 3,226,784 + LRK54_RS14535 0.44 -2.0 3,226,784 + LRK54_RS14535 0.44 -2.4 3,226,784 + LRK54_RS14535 0.44 -0.9 3,226,784 + LRK54_RS14535 0.44 -0.6 3,226,784 + LRK54_RS14535 0.44 -1.9 3,226,784 + LRK54_RS14535 0.44 -0.3 3,226,784 + LRK54_RS14535 0.44 -0.1 3,226,784 + LRK54_RS14535 0.44 -0.6 3,226,784 + LRK54_RS14535 0.44 -1.6 3,226,785 - LRK54_RS14535 0.44 -0.1 3,226,785 - LRK54_RS14535 0.44 +0.7 3,226,785 - LRK54_RS14535 0.44 -0.3 3,226,785 - LRK54_RS14535 0.44 -0.2 3,226,785 - LRK54_RS14535 0.44 -0.5 3,226,785 - LRK54_RS14535 0.44 +0.5 3,226,785 - LRK54_RS14535 0.44 -0.9 3,226,785 - LRK54_RS14535 0.44 -0.3 3,226,790 - LRK54_RS14535 0.47 -0.3 3,226,872 - LRK54_RS14535 0.89 -0.6
Or see this region's nucleotide sequence