Experiment: R2A with FW104 filtrate 0.5 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS13425 and LRK54_RS13430 are separated by 50 nucleotides LRK54_RS13430 and LRK54_RS13435 are separated by 11 nucleotides LRK54_RS13435 and LRK54_RS13440 overlap by 1 nucleotides
LRK54_RS13425: LRK54_RS13425 - flagellar basal-body rod protein FlgF, at 3,007,811 to 3,008,551
_RS13425
LRK54_RS13430: LRK54_RS13430 - flagellar basal-body rod protein FlgG, at 3,008,602 to 3,009,387
_RS13430
LRK54_RS13435: LRK54_RS13435 - flagellar basal body L-ring protein FlgH, at 3,009,399 to 3,010,079
_RS13435
LRK54_RS13440: LRK54_RS13440 - flagellar basal body P-ring protein FlgI, at 3,010,079 to 3,011,302
_RS13440
Position (kb)
3009
3010
3011 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3008.475 kb on - strand, within LRK54_RS13425 at 3008.701 kb on + strand, within LRK54_RS13430 at 3008.701 kb on + strand, within LRK54_RS13430 at 3008.701 kb on + strand, within LRK54_RS13430 at 3008.702 kb on - strand, within LRK54_RS13430 at 3008.702 kb on - strand, within LRK54_RS13430 at 3008.738 kb on - strand, within LRK54_RS13430 at 3008.738 kb on - strand, within LRK54_RS13430 at 3008.738 kb on - strand, within LRK54_RS13430 at 3008.941 kb on + strand, within LRK54_RS13430 at 3008.941 kb on + strand, within LRK54_RS13430 at 3008.941 kb on + strand, within LRK54_RS13430 at 3008.941 kb on + strand, within LRK54_RS13430 at 3008.941 kb on + strand, within LRK54_RS13430 at 3008.942 kb on - strand, within LRK54_RS13430 at 3008.944 kb on - strand, within LRK54_RS13430 at 3009.175 kb on + strand, within LRK54_RS13430 at 3009.176 kb on - strand, within LRK54_RS13430 at 3009.176 kb on - strand, within LRK54_RS13430 at 3009.319 kb on + strand at 3009.319 kb on + strand at 3009.320 kb on - strand at 3009.320 kb on - strand at 3009.549 kb on + strand, within LRK54_RS13435 at 3009.549 kb on + strand, within LRK54_RS13435 at 3009.549 kb on + strand, within LRK54_RS13435 at 3009.549 kb on + strand, within LRK54_RS13435 at 3009.549 kb on + strand, within LRK54_RS13435 at 3009.550 kb on - strand, within LRK54_RS13435 at 3009.550 kb on - strand, within LRK54_RS13435 at 3009.550 kb on - strand, within LRK54_RS13435 at 3009.550 kb on - strand, within LRK54_RS13435 at 3009.550 kb on - strand, within LRK54_RS13435 at 3009.550 kb on - strand, within LRK54_RS13435 at 3009.996 kb on + strand, within LRK54_RS13435 at 3009.996 kb on + strand, within LRK54_RS13435 at 3009.996 kb on + strand, within LRK54_RS13435 at 3009.996 kb on + strand, within LRK54_RS13435 at 3009.996 kb on + strand, within LRK54_RS13435 at 3009.996 kb on + strand, within LRK54_RS13435 at 3009.997 kb on - strand, within LRK54_RS13435 at 3009.997 kb on - strand, within LRK54_RS13435 at 3009.997 kb on - strand, within LRK54_RS13435 at 3009.997 kb on - strand, within LRK54_RS13435 at 3009.997 kb on - strand, within LRK54_RS13435 at 3009.997 kb on - strand, within LRK54_RS13435 at 3009.997 kb on - strand, within LRK54_RS13435 at 3009.997 kb on - strand, within LRK54_RS13435 at 3010.084 kb on + strand at 3010.084 kb on + strand at 3010.085 kb on - strand at 3010.085 kb on - strand at 3010.085 kb on - strand at 3010.085 kb on - strand at 3010.085 kb on - strand at 3010.085 kb on - strand at 3010.085 kb on - strand at 3010.085 kb on - strand at 3010.085 kb on - strand at 3010.169 kb on - strand at 3010.169 kb on - strand at 3010.210 kb on + strand, within LRK54_RS13440 at 3010.210 kb on + strand, within LRK54_RS13440 at 3010.221 kb on + strand, within LRK54_RS13440 at 3010.221 kb on + strand, within LRK54_RS13440 at 3010.222 kb on - strand, within LRK54_RS13440 at 3010.222 kb on - strand, within LRK54_RS13440 at 3010.300 kb on + strand, within LRK54_RS13440 at 3010.300 kb on + strand, within LRK54_RS13440 at 3010.301 kb on - strand, within LRK54_RS13440 at 3010.301 kb on - strand, within LRK54_RS13440 at 3010.301 kb on - strand, within LRK54_RS13440 at 3010.301 kb on - strand, within LRK54_RS13440 at 3010.604 kb on + strand, within LRK54_RS13440 at 3010.604 kb on + strand, within LRK54_RS13440 at 3010.605 kb on - strand, within LRK54_RS13440 at 3010.629 kb on + strand, within LRK54_RS13440 at 3010.830 kb on - strand, within LRK54_RS13440
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with FW104 filtrate 0.5 X remove 3,008,475 - LRK54_RS13425 0.90 +1.2 3,008,701 + LRK54_RS13430 0.13 -0.2 3,008,701 + LRK54_RS13430 0.13 -1.1 3,008,701 + LRK54_RS13430 0.13 +1.8 3,008,702 - LRK54_RS13430 0.13 +0.9 3,008,702 - LRK54_RS13430 0.13 -0.4 3,008,738 - LRK54_RS13430 0.17 +0.7 3,008,738 - LRK54_RS13430 0.17 +0.1 3,008,738 - LRK54_RS13430 0.17 +0.3 3,008,941 + LRK54_RS13430 0.43 +0.9 3,008,941 + LRK54_RS13430 0.43 -0.0 3,008,941 + LRK54_RS13430 0.43 +0.1 3,008,941 + LRK54_RS13430 0.43 +1.0 3,008,941 + LRK54_RS13430 0.43 +0.1 3,008,942 - LRK54_RS13430 0.43 +0.4 3,008,944 - LRK54_RS13430 0.44 +0.8 3,009,175 + LRK54_RS13430 0.73 +0.4 3,009,176 - LRK54_RS13430 0.73 +0.1 3,009,176 - LRK54_RS13430 0.73 -0.1 3,009,319 + -0.1 3,009,319 + +0.6 3,009,320 - +0.1 3,009,320 - +0.4 3,009,549 + LRK54_RS13435 0.22 +0.2 3,009,549 + LRK54_RS13435 0.22 +0.1 3,009,549 + LRK54_RS13435 0.22 +0.4 3,009,549 + LRK54_RS13435 0.22 -0.5 3,009,549 + LRK54_RS13435 0.22 -1.2 3,009,550 - LRK54_RS13435 0.22 +2.0 3,009,550 - LRK54_RS13435 0.22 -0.6 3,009,550 - LRK54_RS13435 0.22 +0.9 3,009,550 - LRK54_RS13435 0.22 +0.5 3,009,550 - LRK54_RS13435 0.22 +0.3 3,009,550 - LRK54_RS13435 0.22 +0.6 3,009,996 + LRK54_RS13435 0.88 +0.7 3,009,996 + LRK54_RS13435 0.88 +0.3 3,009,996 + LRK54_RS13435 0.88 +0.2 3,009,996 + LRK54_RS13435 0.88 +0.1 3,009,996 + LRK54_RS13435 0.88 -1.6 3,009,996 + LRK54_RS13435 0.88 -0.6 3,009,997 - LRK54_RS13435 0.88 +0.6 3,009,997 - LRK54_RS13435 0.88 -0.1 3,009,997 - LRK54_RS13435 0.88 +0.5 3,009,997 - LRK54_RS13435 0.88 +0.2 3,009,997 - LRK54_RS13435 0.88 +2.2 3,009,997 - LRK54_RS13435 0.88 +0.1 3,009,997 - LRK54_RS13435 0.88 -1.6 3,009,997 - LRK54_RS13435 0.88 -0.2 3,010,084 + -1.4 3,010,084 + +0.2 3,010,085 - +0.0 3,010,085 - +0.4 3,010,085 - -0.6 3,010,085 - +0.2 3,010,085 - -0.0 3,010,085 - +1.2 3,010,085 - +0.3 3,010,085 - -0.4 3,010,085 - +0.5 3,010,169 - +0.5 3,010,169 - -0.8 3,010,210 + LRK54_RS13440 0.11 +0.5 3,010,210 + LRK54_RS13440 0.11 -0.4 3,010,221 + LRK54_RS13440 0.12 -0.1 3,010,221 + LRK54_RS13440 0.12 +0.5 3,010,222 - LRK54_RS13440 0.12 -0.6 3,010,222 - LRK54_RS13440 0.12 -1.5 3,010,300 + LRK54_RS13440 0.18 +0.2 3,010,300 + LRK54_RS13440 0.18 -0.8 3,010,301 - LRK54_RS13440 0.18 -0.1 3,010,301 - LRK54_RS13440 0.18 -0.4 3,010,301 - LRK54_RS13440 0.18 +0.3 3,010,301 - LRK54_RS13440 0.18 -3.5 3,010,604 + LRK54_RS13440 0.43 +0.4 3,010,604 + LRK54_RS13440 0.43 +1.0 3,010,605 - LRK54_RS13440 0.43 -2.0 3,010,629 + LRK54_RS13440 0.45 -1.4 3,010,830 - LRK54_RS13440 0.61 -0.0
Or see this region's nucleotide sequence