Experiment: R2A with FW104 filtrate 0.5 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS12845 and LRK54_RS12850 are separated by 150 nucleotides LRK54_RS12850 and LRK54_RS12855 are separated by 8 nucleotides LRK54_RS12855 and LRK54_RS12860 are separated by 37 nucleotides
LRK54_RS12845: LRK54_RS12845 - phosphoenolpyruvate--protein phosphotransferase, at 2,874,673 to 2,876,418
_RS12845
LRK54_RS12850: LRK54_RS12850 - HPr family phosphocarrier protein, at 2,876,569 to 2,876,838
_RS12850
LRK54_RS12855: LRK54_RS12855 - PTS fructose transporter subunit IIA, at 2,876,847 to 2,877,239
_RS12855
LRK54_RS12860: LRK54_RS12860 - RNase adapter RapZ, at 2,877,277 to 2,878,182
_RS12860
Position (kb)
2876
2877 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2875.844 kb on + strand, within LRK54_RS12845 at 2875.844 kb on + strand, within LRK54_RS12845 at 2876.045 kb on + strand, within LRK54_RS12845 at 2876.045 kb on + strand, within LRK54_RS12845 at 2876.045 kb on + strand, within LRK54_RS12845 at 2876.046 kb on - strand, within LRK54_RS12845 at 2876.339 kb on + strand at 2876.339 kb on + strand at 2876.339 kb on + strand at 2876.339 kb on + strand at 2876.339 kb on + strand at 2876.340 kb on - strand at 2876.340 kb on - strand at 2876.340 kb on - strand at 2876.340 kb on - strand at 2876.340 kb on - strand at 2876.340 kb on - strand at 2876.340 kb on - strand at 2876.340 kb on - strand at 2876.450 kb on + strand at 2876.450 kb on + strand at 2876.450 kb on + strand at 2876.450 kb on + strand at 2876.450 kb on + strand at 2876.450 kb on + strand at 2876.451 kb on - strand at 2876.471 kb on + strand at 2876.471 kb on + strand at 2876.471 kb on + strand at 2876.471 kb on + strand at 2876.471 kb on + strand at 2876.471 kb on + strand at 2876.472 kb on - strand at 2876.472 kb on - strand at 2876.472 kb on - strand at 2876.472 kb on - strand at 2876.472 kb on - strand at 2876.770 kb on + strand, within LRK54_RS12850 at 2876.770 kb on + strand, within LRK54_RS12850 at 2876.770 kb on + strand, within LRK54_RS12850 at 2876.770 kb on + strand, within LRK54_RS12850 at 2876.770 kb on + strand, within LRK54_RS12850 at 2876.771 kb on - strand, within LRK54_RS12850 at 2876.771 kb on - strand, within LRK54_RS12850 at 2876.771 kb on - strand, within LRK54_RS12850 at 2876.771 kb on - strand, within LRK54_RS12850 at 2876.771 kb on - strand, within LRK54_RS12850 at 2876.771 kb on - strand, within LRK54_RS12850 at 2876.771 kb on - strand, within LRK54_RS12850 at 2876.771 kb on - strand, within LRK54_RS12850 at 2876.771 kb on - strand, within LRK54_RS12850 at 2876.771 kb on - strand, within LRK54_RS12850 at 2876.926 kb on + strand, within LRK54_RS12855 at 2876.926 kb on + strand, within LRK54_RS12855 at 2876.926 kb on + strand, within LRK54_RS12855 at 2876.926 kb on + strand, within LRK54_RS12855 at 2876.926 kb on + strand, within LRK54_RS12855 at 2876.926 kb on + strand, within LRK54_RS12855 at 2876.927 kb on - strand, within LRK54_RS12855 at 2876.927 kb on - strand, within LRK54_RS12855 at 2876.934 kb on + strand, within LRK54_RS12855 at 2876.934 kb on + strand, within LRK54_RS12855 at 2876.934 kb on + strand, within LRK54_RS12855 at 2876.982 kb on + strand, within LRK54_RS12855 at 2876.983 kb on - strand, within LRK54_RS12855 at 2877.031 kb on + strand, within LRK54_RS12855 at 2877.031 kb on + strand, within LRK54_RS12855 at 2877.031 kb on + strand, within LRK54_RS12855 at 2877.031 kb on + strand, within LRK54_RS12855 at 2877.031 kb on + strand at 2877.031 kb on + strand, within LRK54_RS12855 at 2877.032 kb on - strand, within LRK54_RS12855 at 2877.032 kb on - strand, within LRK54_RS12855 at 2877.051 kb on + strand, within LRK54_RS12855 at 2877.051 kb on + strand, within LRK54_RS12855 at 2877.225 kb on + strand at 2877.225 kb on + strand at 2877.225 kb on + strand at 2877.225 kb on + strand at 2877.225 kb on + strand at 2877.225 kb on + strand at 2877.225 kb on + strand at 2877.226 kb on - strand at 2877.326 kb on + strand at 2877.353 kb on + strand at 2877.353 kb on + strand at 2877.354 kb on - strand at 2877.354 kb on - strand at 2877.401 kb on + strand, within LRK54_RS12860 at 2877.401 kb on + strand, within LRK54_RS12860 at 2877.401 kb on + strand, within LRK54_RS12860 at 2877.401 kb on + strand, within LRK54_RS12860 at 2877.401 kb on + strand, within LRK54_RS12860 at 2877.401 kb on + strand, within LRK54_RS12860 at 2877.402 kb on - strand, within LRK54_RS12860 at 2877.402 kb on - strand, within LRK54_RS12860 at 2877.402 kb on - strand, within LRK54_RS12860 at 2877.402 kb on - strand, within LRK54_RS12860 at 2877.402 kb on - strand, within LRK54_RS12860 at 2877.402 kb on - strand, within LRK54_RS12860 at 2877.402 kb on - strand, within LRK54_RS12860 at 2877.402 kb on - strand, within LRK54_RS12860 at 2877.402 kb on - strand, within LRK54_RS12860 at 2877.402 kb on - strand, within LRK54_RS12860 at 2877.402 kb on - strand, within LRK54_RS12860 at 2877.402 kb on - strand, within LRK54_RS12860 at 2877.402 kb on - strand, within LRK54_RS12860 at 2877.467 kb on + strand, within LRK54_RS12860 at 2877.467 kb on + strand, within LRK54_RS12860 at 2877.468 kb on - strand, within LRK54_RS12860 at 2877.468 kb on - strand, within LRK54_RS12860 at 2877.468 kb on - strand, within LRK54_RS12860 at 2877.830 kb on + strand, within LRK54_RS12860 at 2877.830 kb on + strand, within LRK54_RS12860 at 2877.831 kb on - strand, within LRK54_RS12860
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with FW104 filtrate 0.5 X remove 2,875,844 + LRK54_RS12845 0.67 +0.2 2,875,844 + LRK54_RS12845 0.67 -0.4 2,876,045 + LRK54_RS12845 0.79 +0.0 2,876,045 + LRK54_RS12845 0.79 +0.6 2,876,045 + LRK54_RS12845 0.79 +0.5 2,876,046 - LRK54_RS12845 0.79 +0.5 2,876,339 + +0.4 2,876,339 + +0.1 2,876,339 + -0.6 2,876,339 + +0.1 2,876,339 + +0.6 2,876,340 - -0.6 2,876,340 - -1.0 2,876,340 - -0.7 2,876,340 - -0.3 2,876,340 - +0.6 2,876,340 - -0.9 2,876,340 - -0.5 2,876,340 - +0.8 2,876,450 + +1.6 2,876,450 + +0.5 2,876,450 + +0.1 2,876,450 + +0.6 2,876,450 + -0.4 2,876,450 + +1.2 2,876,451 - +0.1 2,876,471 + +0.9 2,876,471 + +0.2 2,876,471 + -0.8 2,876,471 + -0.0 2,876,471 + +0.3 2,876,471 + +0.4 2,876,472 - -0.1 2,876,472 - +2.8 2,876,472 - -0.4 2,876,472 - -0.6 2,876,472 - -0.3 2,876,770 + LRK54_RS12850 0.74 +0.3 2,876,770 + LRK54_RS12850 0.74 -1.0 2,876,770 + LRK54_RS12850 0.74 +2.2 2,876,770 + LRK54_RS12850 0.74 +2.2 2,876,770 + LRK54_RS12850 0.74 -0.6 2,876,771 - LRK54_RS12850 0.75 +0.2 2,876,771 - LRK54_RS12850 0.75 +0.1 2,876,771 - LRK54_RS12850 0.75 +0.1 2,876,771 - LRK54_RS12850 0.75 +0.9 2,876,771 - LRK54_RS12850 0.75 -0.8 2,876,771 - LRK54_RS12850 0.75 +1.2 2,876,771 - LRK54_RS12850 0.75 -0.0 2,876,771 - LRK54_RS12850 0.75 +1.2 2,876,771 - LRK54_RS12850 0.75 +1.1 2,876,771 - LRK54_RS12850 0.75 +2.2 2,876,926 + LRK54_RS12855 0.20 -0.4 2,876,926 + LRK54_RS12855 0.20 -1.4 2,876,926 + LRK54_RS12855 0.20 -2.4 2,876,926 + LRK54_RS12855 0.20 -0.1 2,876,926 + LRK54_RS12855 0.20 -0.9 2,876,926 + LRK54_RS12855 0.20 -1.6 2,876,927 - LRK54_RS12855 0.20 -0.1 2,876,927 - LRK54_RS12855 0.20 -1.2 2,876,934 + LRK54_RS12855 0.22 -2.2 2,876,934 + LRK54_RS12855 0.22 -3.2 2,876,934 + LRK54_RS12855 0.22 -2.1 2,876,982 + LRK54_RS12855 0.34 +0.2 2,876,983 - LRK54_RS12855 0.35 -1.6 2,877,031 + LRK54_RS12855 0.47 -2.2 2,877,031 + LRK54_RS12855 0.47 -0.4 2,877,031 + LRK54_RS12855 0.47 -0.8 2,877,031 + LRK54_RS12855 0.47 -0.1 2,877,031 + -0.1 2,877,031 + LRK54_RS12855 0.47 -1.5 2,877,032 - LRK54_RS12855 0.47 -1.4 2,877,032 - LRK54_RS12855 0.47 -0.1 2,877,051 + LRK54_RS12855 0.52 -1.5 2,877,051 + LRK54_RS12855 0.52 -2.1 2,877,225 + -0.9 2,877,225 + -0.1 2,877,225 + -2.6 2,877,225 + -0.1 2,877,225 + -1.8 2,877,225 + -2.9 2,877,225 + +0.2 2,877,226 - -2.0 2,877,326 + -0.6 2,877,353 + -0.1 2,877,353 + -1.1 2,877,354 - -0.8 2,877,354 - -0.4 2,877,401 + LRK54_RS12860 0.14 -1.2 2,877,401 + LRK54_RS12860 0.14 -2.0 2,877,401 + LRK54_RS12860 0.14 -0.8 2,877,401 + LRK54_RS12860 0.14 -2.5 2,877,401 + LRK54_RS12860 0.14 -0.8 2,877,401 + LRK54_RS12860 0.14 -1.8 2,877,402 - LRK54_RS12860 0.14 -0.1 2,877,402 - LRK54_RS12860 0.14 -1.2 2,877,402 - LRK54_RS12860 0.14 +1.2 2,877,402 - LRK54_RS12860 0.14 -0.4 2,877,402 - LRK54_RS12860 0.14 -0.1 2,877,402 - LRK54_RS12860 0.14 +0.2 2,877,402 - LRK54_RS12860 0.14 -0.8 2,877,402 - LRK54_RS12860 0.14 -0.8 2,877,402 - LRK54_RS12860 0.14 +0.6 2,877,402 - LRK54_RS12860 0.14 -1.2 2,877,402 - LRK54_RS12860 0.14 -1.6 2,877,402 - LRK54_RS12860 0.14 -1.7 2,877,402 - LRK54_RS12860 0.14 -1.7 2,877,467 + LRK54_RS12860 0.21 -1.4 2,877,467 + LRK54_RS12860 0.21 +0.2 2,877,468 - LRK54_RS12860 0.21 -0.9 2,877,468 - LRK54_RS12860 0.21 -1.5 2,877,468 - LRK54_RS12860 0.21 -0.6 2,877,830 + LRK54_RS12860 0.61 -0.9 2,877,830 + LRK54_RS12860 0.61 -1.8 2,877,831 - LRK54_RS12860 0.61 -0.1
Or see this region's nucleotide sequence