Experiment: R2A with FW104 filtrate 0.5 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS05530 and LRK54_RS05535 are separated by 18 nucleotides LRK54_RS05535 and LRK54_RS05540 overlap by 8 nucleotides LRK54_RS05540 and LRK54_RS05545 are separated by 325 nucleotides
LRK54_RS05530: LRK54_RS05530 - electron transfer flavoprotein-ubiquinone oxidoreductase, at 1,194,327 to 1,195,937
_RS05530
LRK54_RS05535: LRK54_RS05535 - glycosyltransferase family 2 protein, at 1,195,956 to 1,196,894
_RS05535
LRK54_RS05540: LRK54_RS05540 - glycosyltransferase family 2 protein, at 1,196,887 to 1,197,873
_RS05540
LRK54_RS05545: LRK54_RS05545 - electron transfer flavoprotein subunit beta/FixA family protein, at 1,198,199 to 1,198,945
_RS05545
Position (kb)
1196
1197
1198 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1195.923 kb on + strand at 1195.923 kb on + strand at 1195.923 kb on + strand at 1195.923 kb on + strand at 1195.924 kb on - strand at 1195.924 kb on - strand at 1195.924 kb on - strand at 1195.935 kb on + strand at 1195.935 kb on + strand at 1195.935 kb on + strand at 1195.935 kb on + strand at 1195.935 kb on + strand at 1195.935 kb on + strand at 1195.935 kb on + strand at 1195.935 kb on + strand at 1195.935 kb on + strand at 1195.935 kb on + strand at 1195.936 kb on - strand at 1195.936 kb on - strand at 1195.936 kb on - strand at 1195.936 kb on - strand at 1195.936 kb on - strand at 1195.936 kb on - strand at 1195.936 kb on - strand at 1195.936 kb on - strand at 1196.170 kb on + strand, within LRK54_RS05535 at 1196.170 kb on + strand, within LRK54_RS05535 at 1196.170 kb on + strand, within LRK54_RS05535 at 1196.170 kb on + strand, within LRK54_RS05535 at 1196.170 kb on + strand, within LRK54_RS05535 at 1196.170 kb on + strand, within LRK54_RS05535 at 1196.170 kb on + strand, within LRK54_RS05535 at 1196.170 kb on + strand, within LRK54_RS05535 at 1196.170 kb on + strand, within LRK54_RS05535 at 1196.171 kb on - strand, within LRK54_RS05535 at 1196.171 kb on - strand, within LRK54_RS05535 at 1196.171 kb on - strand, within LRK54_RS05535 at 1196.171 kb on - strand, within LRK54_RS05535 at 1196.171 kb on - strand, within LRK54_RS05535 at 1196.171 kb on - strand, within LRK54_RS05535 at 1196.171 kb on - strand, within LRK54_RS05535 at 1196.171 kb on - strand, within LRK54_RS05535 at 1196.171 kb on - strand, within LRK54_RS05535 at 1196.171 kb on - strand, within LRK54_RS05535 at 1196.171 kb on - strand, within LRK54_RS05535 at 1196.173 kb on + strand, within LRK54_RS05535 at 1196.173 kb on + strand, within LRK54_RS05535 at 1196.173 kb on + strand, within LRK54_RS05535 at 1196.173 kb on + strand, within LRK54_RS05535 at 1196.173 kb on + strand, within LRK54_RS05535 at 1196.173 kb on + strand, within LRK54_RS05535 at 1196.174 kb on - strand, within LRK54_RS05535 at 1196.174 kb on - strand, within LRK54_RS05535 at 1196.174 kb on - strand, within LRK54_RS05535 at 1196.174 kb on - strand, within LRK54_RS05535 at 1196.174 kb on - strand, within LRK54_RS05535 at 1196.174 kb on - strand, within LRK54_RS05535 at 1196.174 kb on - strand, within LRK54_RS05535 at 1196.174 kb on - strand, within LRK54_RS05535 at 1196.251 kb on + strand, within LRK54_RS05535 at 1196.251 kb on + strand, within LRK54_RS05535 at 1196.251 kb on + strand, within LRK54_RS05535 at 1196.251 kb on + strand, within LRK54_RS05535 at 1196.251 kb on + strand, within LRK54_RS05535 at 1196.251 kb on + strand, within LRK54_RS05535 at 1196.252 kb on - strand, within LRK54_RS05535 at 1196.252 kb on - strand, within LRK54_RS05535 at 1196.252 kb on - strand, within LRK54_RS05535 at 1196.252 kb on - strand, within LRK54_RS05535 at 1196.252 kb on - strand, within LRK54_RS05535 at 1196.252 kb on - strand, within LRK54_RS05535 at 1196.252 kb on - strand, within LRK54_RS05535 at 1196.252 kb on - strand, within LRK54_RS05535 at 1196.252 kb on - strand, within LRK54_RS05535 at 1196.268 kb on + strand, within LRK54_RS05535 at 1196.268 kb on + strand, within LRK54_RS05535 at 1196.268 kb on + strand, within LRK54_RS05535 at 1196.268 kb on + strand, within LRK54_RS05535 at 1196.269 kb on - strand, within LRK54_RS05535 at 1196.269 kb on - strand, within LRK54_RS05535 at 1196.269 kb on - strand, within LRK54_RS05535 at 1196.269 kb on - strand, within LRK54_RS05535 at 1196.269 kb on - strand, within LRK54_RS05535 at 1196.284 kb on + strand, within LRK54_RS05535 at 1196.284 kb on + strand, within LRK54_RS05535 at 1196.848 kb on + strand at 1196.848 kb on + strand at 1196.848 kb on + strand at 1196.849 kb on - strand at 1196.849 kb on - strand at 1196.849 kb on - strand at 1196.849 kb on - strand at 1196.849 kb on - strand at 1197.225 kb on - strand, within LRK54_RS05540 at 1197.574 kb on + strand, within LRK54_RS05540 at 1197.574 kb on + strand, within LRK54_RS05540 at 1197.574 kb on + strand at 1197.574 kb on + strand, within LRK54_RS05540 at 1197.574 kb on + strand, within LRK54_RS05540 at 1197.574 kb on + strand, within LRK54_RS05540 at 1197.574 kb on + strand, within LRK54_RS05540 at 1197.574 kb on + strand, within LRK54_RS05540 at 1197.575 kb on - strand, within LRK54_RS05540 at 1197.575 kb on - strand, within LRK54_RS05540 at 1197.575 kb on - strand, within LRK54_RS05540 at 1197.575 kb on - strand, within LRK54_RS05540 at 1197.575 kb on - strand, within LRK54_RS05540 at 1197.575 kb on - strand, within LRK54_RS05540 at 1197.575 kb on - strand, within LRK54_RS05540 at 1197.575 kb on - strand, within LRK54_RS05540 at 1197.575 kb on - strand, within LRK54_RS05540 at 1197.575 kb on - strand, within LRK54_RS05540 at 1197.575 kb on - strand, within LRK54_RS05540 at 1197.647 kb on + strand, within LRK54_RS05540 at 1197.648 kb on - strand, within LRK54_RS05540 at 1197.648 kb on - strand, within LRK54_RS05540 at 1197.648 kb on - strand, within LRK54_RS05540 at 1197.726 kb on + strand, within LRK54_RS05540 at 1197.726 kb on + strand, within LRK54_RS05540 at 1197.726 kb on + strand, within LRK54_RS05540 at 1197.726 kb on + strand, within LRK54_RS05540 at 1197.727 kb on - strand, within LRK54_RS05540 at 1197.727 kb on - strand, within LRK54_RS05540 at 1197.727 kb on - strand, within LRK54_RS05540 at 1197.821 kb on + strand at 1197.821 kb on + strand at 1197.821 kb on + strand at 1197.821 kb on + strand at 1197.821 kb on + strand at 1197.821 kb on + strand at 1197.822 kb on - strand at 1197.822 kb on - strand at 1197.822 kb on - strand at 1197.822 kb on - strand at 1197.822 kb on - strand at 1197.822 kb on - strand at 1197.822 kb on - strand at 1197.836 kb on + strand at 1197.836 kb on + strand at 1197.836 kb on + strand at 1197.836 kb on + strand at 1197.836 kb on + strand at 1197.836 kb on + strand at 1197.837 kb on - strand at 1197.837 kb on - strand at 1197.837 kb on - strand at 1197.837 kb on - strand at 1197.837 kb on - strand at 1197.934 kb on - strand at 1197.971 kb on + strand at 1197.972 kb on - strand at 1197.972 kb on - strand at 1198.063 kb on + strand at 1198.063 kb on + strand at 1198.063 kb on + strand at 1198.063 kb on + strand at 1198.064 kb on - strand at 1198.064 kb on - strand at 1198.064 kb on - strand at 1198.064 kb on - strand at 1198.125 kb on + strand at 1198.125 kb on + strand at 1198.125 kb on + strand at 1198.125 kb on + strand at 1198.125 kb on + strand at 1198.125 kb on + strand at 1198.126 kb on - strand at 1198.126 kb on - strand at 1198.126 kb on - strand at 1198.145 kb on + strand at 1198.146 kb on - strand at 1198.146 kb on - strand at 1198.146 kb on - strand at 1198.218 kb on - strand at 1198.235 kb on + strand at 1198.235 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with FW104 filtrate 0.5 X remove 1,195,923 + +0.3 1,195,923 + +0.3 1,195,923 + +0.2 1,195,923 + -0.8 1,195,924 - -1.4 1,195,924 - -2.0 1,195,924 - +0.1 1,195,935 + -0.5 1,195,935 + -0.2 1,195,935 + -0.0 1,195,935 + +0.1 1,195,935 + +0.2 1,195,935 + -0.1 1,195,935 + +1.0 1,195,935 + -0.5 1,195,935 + -0.6 1,195,935 + -0.3 1,195,936 - -0.4 1,195,936 - -0.9 1,195,936 - -1.7 1,195,936 - -0.2 1,195,936 - -1.7 1,195,936 - -0.4 1,195,936 - -0.6 1,195,936 - -0.3 1,196,170 + LRK54_RS05535 0.23 +0.1 1,196,170 + LRK54_RS05535 0.23 +0.1 1,196,170 + LRK54_RS05535 0.23 -0.2 1,196,170 + LRK54_RS05535 0.23 -0.9 1,196,170 + LRK54_RS05535 0.23 +1.3 1,196,170 + LRK54_RS05535 0.23 -0.3 1,196,170 + LRK54_RS05535 0.23 -1.0 1,196,170 + LRK54_RS05535 0.23 -1.4 1,196,170 + LRK54_RS05535 0.23 +0.8 1,196,171 - LRK54_RS05535 0.23 -0.3 1,196,171 - LRK54_RS05535 0.23 -1.0 1,196,171 - LRK54_RS05535 0.23 -0.3 1,196,171 - LRK54_RS05535 0.23 -2.1 1,196,171 - LRK54_RS05535 0.23 +0.1 1,196,171 - LRK54_RS05535 0.23 +0.7 1,196,171 - LRK54_RS05535 0.23 -1.5 1,196,171 - LRK54_RS05535 0.23 -0.2 1,196,171 - LRK54_RS05535 0.23 -0.9 1,196,171 - LRK54_RS05535 0.23 +0.2 1,196,171 - LRK54_RS05535 0.23 -1.0 1,196,173 + LRK54_RS05535 0.23 -1.3 1,196,173 + LRK54_RS05535 0.23 -0.5 1,196,173 + LRK54_RS05535 0.23 -0.5 1,196,173 + LRK54_RS05535 0.23 -1.2 1,196,173 + LRK54_RS05535 0.23 -0.9 1,196,173 + LRK54_RS05535 0.23 +1.0 1,196,174 - LRK54_RS05535 0.23 -0.0 1,196,174 - LRK54_RS05535 0.23 +0.7 1,196,174 - LRK54_RS05535 0.23 -0.0 1,196,174 - LRK54_RS05535 0.23 +0.9 1,196,174 - LRK54_RS05535 0.23 -0.5 1,196,174 - LRK54_RS05535 0.23 +0.7 1,196,174 - LRK54_RS05535 0.23 -0.9 1,196,174 - LRK54_RS05535 0.23 -0.5 1,196,251 + LRK54_RS05535 0.31 -1.6 1,196,251 + LRK54_RS05535 0.31 +1.7 1,196,251 + LRK54_RS05535 0.31 +0.1 1,196,251 + LRK54_RS05535 0.31 -0.5 1,196,251 + LRK54_RS05535 0.31 +0.3 1,196,251 + LRK54_RS05535 0.31 +0.5 1,196,252 - LRK54_RS05535 0.32 -0.3 1,196,252 - LRK54_RS05535 0.32 -0.7 1,196,252 - LRK54_RS05535 0.32 +0.7 1,196,252 - LRK54_RS05535 0.32 +1.0 1,196,252 - LRK54_RS05535 0.32 -0.5 1,196,252 - LRK54_RS05535 0.32 -0.6 1,196,252 - LRK54_RS05535 0.32 -1.0 1,196,252 - LRK54_RS05535 0.32 -0.3 1,196,252 - LRK54_RS05535 0.32 -0.7 1,196,268 + LRK54_RS05535 0.33 -0.7 1,196,268 + LRK54_RS05535 0.33 +0.1 1,196,268 + LRK54_RS05535 0.33 -0.6 1,196,268 + LRK54_RS05535 0.33 -1.6 1,196,269 - LRK54_RS05535 0.33 +1.0 1,196,269 - LRK54_RS05535 0.33 -0.3 1,196,269 - LRK54_RS05535 0.33 +1.1 1,196,269 - LRK54_RS05535 0.33 -0.3 1,196,269 - LRK54_RS05535 0.33 -0.3 1,196,284 + LRK54_RS05535 0.35 +0.9 1,196,284 + LRK54_RS05535 0.35 +0.7 1,196,848 + -0.0 1,196,848 + -0.5 1,196,848 + -0.4 1,196,849 - -0.1 1,196,849 - -1.1 1,196,849 - -0.7 1,196,849 - -1.2 1,196,849 - -0.5 1,197,225 - LRK54_RS05540 0.34 -0.0 1,197,574 + LRK54_RS05540 0.70 +1.1 1,197,574 + LRK54_RS05540 0.70 +0.3 1,197,574 + +0.7 1,197,574 + LRK54_RS05540 0.70 -0.9 1,197,574 + LRK54_RS05540 0.70 +1.3 1,197,574 + LRK54_RS05540 0.70 -0.6 1,197,574 + LRK54_RS05540 0.70 -0.3 1,197,574 + LRK54_RS05540 0.70 +0.3 1,197,575 - LRK54_RS05540 0.70 -0.5 1,197,575 - LRK54_RS05540 0.70 -0.5 1,197,575 - LRK54_RS05540 0.70 -1.6 1,197,575 - LRK54_RS05540 0.70 +0.7 1,197,575 - LRK54_RS05540 0.70 +0.7 1,197,575 - LRK54_RS05540 0.70 +0.7 1,197,575 - LRK54_RS05540 0.70 +2.3 1,197,575 - LRK54_RS05540 0.70 -1.8 1,197,575 - LRK54_RS05540 0.70 +0.5 1,197,575 - LRK54_RS05540 0.70 -0.3 1,197,575 - LRK54_RS05540 0.70 +0.3 1,197,647 + LRK54_RS05540 0.77 -1.3 1,197,648 - LRK54_RS05540 0.77 -0.3 1,197,648 - LRK54_RS05540 0.77 -0.7 1,197,648 - LRK54_RS05540 0.77 -0.0 1,197,726 + LRK54_RS05540 0.85 -0.7 1,197,726 + LRK54_RS05540 0.85 +0.3 1,197,726 + LRK54_RS05540 0.85 +0.3 1,197,726 + LRK54_RS05540 0.85 -0.5 1,197,727 - LRK54_RS05540 0.85 -0.8 1,197,727 - LRK54_RS05540 0.85 -0.5 1,197,727 - LRK54_RS05540 0.85 -0.6 1,197,821 + -0.9 1,197,821 + -0.7 1,197,821 + +0.9 1,197,821 + -1.2 1,197,821 + +0.7 1,197,821 + -0.1 1,197,822 - +0.3 1,197,822 - -0.1 1,197,822 - -1.5 1,197,822 - +2.9 1,197,822 - +0.7 1,197,822 - -0.5 1,197,822 - +1.3 1,197,836 + +0.7 1,197,836 + +0.9 1,197,836 + +1.7 1,197,836 + -0.5 1,197,836 + +0.3 1,197,836 + +2.9 1,197,837 - -0.9 1,197,837 - -0.5 1,197,837 - +1.0 1,197,837 - +0.7 1,197,837 - -0.0 1,197,934 - -0.4 1,197,971 + +0.7 1,197,972 - -0.4 1,197,972 - -0.7 1,198,063 + -0.9 1,198,063 + -0.2 1,198,063 + -0.0 1,198,063 + +0.5 1,198,064 - -0.9 1,198,064 - -1.3 1,198,064 - +0.9 1,198,064 - -0.5 1,198,125 + -0.1 1,198,125 + -1.0 1,198,125 + -0.7 1,198,125 + +0.5 1,198,125 + -1.4 1,198,125 + -1.1 1,198,126 - -0.3 1,198,126 - +0.3 1,198,126 - -0.7 1,198,145 + -0.3 1,198,146 - -0.0 1,198,146 - +0.5 1,198,146 - -0.3 1,198,218 - +1.3 1,198,235 + +0.3 1,198,235 + -1.0
Or see this region's nucleotide sequence