Experiment: R2A with FW104 filtrate 0.5 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS03850 and LRK54_RS03855 are separated by 124 nucleotides LRK54_RS03855 and LRK54_RS03860 are separated by 61 nucleotides LRK54_RS03860 and LRK54_RS03865 are separated by 47 nucleotides
LRK54_RS03850: LRK54_RS03850 - NAD(P) transhydrogenase subunit alpha, at 809,539 to 810,642
_RS03850
LRK54_RS03855: LRK54_RS03855 - cytidine deaminase, at 810,767 to 811,183
_RS03855
LRK54_RS03860: LRK54_RS03860 - nitroreductase, at 811,245 to 811,814
_RS03860
LRK54_RS03865: LRK54_RS03865 - exodeoxyribonuclease IX, at 811,862 to 812,716
_RS03865
Position (kb)
811
812 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 810.276 kb on + strand, within LRK54_RS03850 at 810.353 kb on + strand, within LRK54_RS03850 at 810.354 kb on - strand, within LRK54_RS03850 at 810.382 kb on - strand, within LRK54_RS03850 at 810.382 kb on - strand, within LRK54_RS03850 at 810.436 kb on + strand, within LRK54_RS03850 at 810.436 kb on + strand, within LRK54_RS03850 at 810.436 kb on + strand, within LRK54_RS03850 at 810.436 kb on + strand, within LRK54_RS03850 at 810.436 kb on + strand, within LRK54_RS03850 at 810.436 kb on + strand, within LRK54_RS03850 at 810.436 kb on + strand, within LRK54_RS03850 at 810.436 kb on + strand, within LRK54_RS03850 at 810.437 kb on - strand, within LRK54_RS03850 at 810.437 kb on - strand, within LRK54_RS03850 at 810.437 kb on - strand, within LRK54_RS03850 at 810.437 kb on - strand, within LRK54_RS03850 at 810.437 kb on - strand, within LRK54_RS03850 at 810.437 kb on - strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.488 kb on + strand, within LRK54_RS03850 at 810.489 kb on - strand, within LRK54_RS03850 at 810.489 kb on - strand, within LRK54_RS03850 at 810.489 kb on - strand, within LRK54_RS03850 at 810.489 kb on - strand, within LRK54_RS03850 at 810.489 kb on - strand, within LRK54_RS03850 at 810.538 kb on + strand at 810.538 kb on + strand at 810.538 kb on + strand at 810.539 kb on - strand at 810.539 kb on - strand at 810.539 kb on - strand at 810.539 kb on - strand at 810.629 kb on - strand at 810.675 kb on + strand at 810.675 kb on + strand at 810.675 kb on + strand at 810.675 kb on + strand at 810.676 kb on - strand at 810.676 kb on - strand at 810.676 kb on - strand at 810.676 kb on - strand at 810.717 kb on + strand at 810.717 kb on + strand at 810.717 kb on + strand at 810.717 kb on + strand at 810.717 kb on + strand at 810.717 kb on + strand at 810.717 kb on + strand at 810.717 kb on + strand at 810.717 kb on + strand at 810.718 kb on - strand at 810.718 kb on - strand at 810.718 kb on - strand at 810.718 kb on - strand at 810.718 kb on - strand at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.833 kb on + strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.834 kb on - strand, within LRK54_RS03855 at 810.842 kb on + strand, within LRK54_RS03855 at 810.842 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.923 kb on + strand, within LRK54_RS03855 at 810.924 kb on - strand, within LRK54_RS03855 at 810.924 kb on - strand, within LRK54_RS03855 at 810.924 kb on - strand, within LRK54_RS03855 at 810.924 kb on - strand, within LRK54_RS03855 at 810.924 kb on - strand, within LRK54_RS03855 at 810.924 kb on - strand, within LRK54_RS03855 at 811.211 kb on + strand at 811.212 kb on - strand at 811.420 kb on + strand, within LRK54_RS03860 at 811.420 kb on + strand, within LRK54_RS03860 at 811.420 kb on + strand, within LRK54_RS03860 at 811.420 kb on + strand, within LRK54_RS03860 at 811.421 kb on - strand, within LRK54_RS03860 at 811.421 kb on - strand, within LRK54_RS03860 at 811.506 kb on + strand, within LRK54_RS03860 at 811.506 kb on + strand, within LRK54_RS03860 at 811.506 kb on + strand, within LRK54_RS03860 at 811.507 kb on - strand, within LRK54_RS03860 at 811.507 kb on - strand, within LRK54_RS03860 at 811.507 kb on - strand, within LRK54_RS03860 at 811.626 kb on + strand, within LRK54_RS03860 at 811.626 kb on + strand, within LRK54_RS03860 at 811.626 kb on + strand, within LRK54_RS03860 at 811.626 kb on + strand, within LRK54_RS03860 at 811.627 kb on - strand, within LRK54_RS03860 at 811.627 kb on - strand, within LRK54_RS03860 at 811.671 kb on + strand, within LRK54_RS03860 at 811.671 kb on + strand, within LRK54_RS03860 at 811.760 kb on - strand at 811.865 kb on + strand at 811.865 kb on + strand at 811.865 kb on + strand at 811.865 kb on + strand at 811.865 kb on + strand at 811.865 kb on + strand at 811.865 kb on + strand at 811.865 kb on + strand at 811.865 kb on + strand at 811.865 kb on + strand at 811.866 kb on - strand at 811.866 kb on - strand at 811.866 kb on - strand at 811.866 kb on - strand at 811.866 kb on - strand at 811.866 kb on - strand at 811.866 kb on - strand at 811.866 kb on - strand at 811.943 kb on + strand at 811.943 kb on + strand at 812.042 kb on + strand, within LRK54_RS03865 at 812.042 kb on + strand, within LRK54_RS03865 at 812.043 kb on - strand, within LRK54_RS03865 at 812.051 kb on + strand, within LRK54_RS03865 at 812.051 kb on + strand, within LRK54_RS03865 at 812.051 kb on + strand, within LRK54_RS03865 at 812.051 kb on + strand, within LRK54_RS03865 at 812.052 kb on - strand, within LRK54_RS03865 at 812.052 kb on - strand, within LRK54_RS03865 at 812.052 kb on - strand, within LRK54_RS03865 at 812.052 kb on - strand, within LRK54_RS03865 at 812.052 kb on - strand, within LRK54_RS03865 at 812.052 kb on - strand, within LRK54_RS03865 at 812.052 kb on - strand, within LRK54_RS03865 at 812.282 kb on + strand, within LRK54_RS03865 at 812.282 kb on + strand, within LRK54_RS03865 at 812.283 kb on - strand, within LRK54_RS03865 at 812.283 kb on - strand, within LRK54_RS03865 at 812.283 kb on - strand, within LRK54_RS03865 at 812.357 kb on + strand, within LRK54_RS03865 at 812.357 kb on + strand, within LRK54_RS03865 at 812.357 kb on + strand, within LRK54_RS03865 at 812.358 kb on - strand, within LRK54_RS03865 at 812.358 kb on - strand, within LRK54_RS03865 at 812.358 kb on - strand, within LRK54_RS03865 at 812.367 kb on + strand, within LRK54_RS03865 at 812.367 kb on + strand, within LRK54_RS03865 at 812.368 kb on - strand, within LRK54_RS03865 at 812.368 kb on - strand, within LRK54_RS03865 at 812.368 kb on - strand, within LRK54_RS03865
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with FW104 filtrate 0.5 X remove 810,276 + LRK54_RS03850 0.67 +0.1 810,353 + LRK54_RS03850 0.74 -1.7 810,354 - LRK54_RS03850 0.74 -0.7 810,382 - LRK54_RS03850 0.76 -0.5 810,382 - LRK54_RS03850 0.76 -0.0 810,436 + LRK54_RS03850 0.81 -1.2 810,436 + LRK54_RS03850 0.81 -2.0 810,436 + LRK54_RS03850 0.81 +0.3 810,436 + LRK54_RS03850 0.81 -0.1 810,436 + LRK54_RS03850 0.81 +1.3 810,436 + LRK54_RS03850 0.81 -0.6 810,436 + LRK54_RS03850 0.81 -0.6 810,436 + LRK54_RS03850 0.81 +1.4 810,437 - LRK54_RS03850 0.81 -0.2 810,437 - LRK54_RS03850 0.81 +0.5 810,437 - LRK54_RS03850 0.81 +0.1 810,437 - LRK54_RS03850 0.81 -0.0 810,437 - LRK54_RS03850 0.81 +0.1 810,437 - LRK54_RS03850 0.81 -0.9 810,488 + LRK54_RS03850 0.86 -0.5 810,488 + LRK54_RS03850 0.86 -1.1 810,488 + LRK54_RS03850 0.86 +0.0 810,488 + LRK54_RS03850 0.86 -1.5 810,488 + LRK54_RS03850 0.86 -0.7 810,488 + LRK54_RS03850 0.86 -1.2 810,488 + LRK54_RS03850 0.86 -0.6 810,488 + LRK54_RS03850 0.86 -0.2 810,488 + LRK54_RS03850 0.86 +0.6 810,488 + LRK54_RS03850 0.86 -0.5 810,489 - LRK54_RS03850 0.86 +0.5 810,489 - LRK54_RS03850 0.86 -0.0 810,489 - LRK54_RS03850 0.86 -0.8 810,489 - LRK54_RS03850 0.86 -0.9 810,489 - LRK54_RS03850 0.86 -0.6 810,538 + -0.8 810,538 + -0.0 810,538 + +0.6 810,539 - -0.2 810,539 - +0.1 810,539 - -0.5 810,539 - +1.4 810,629 - +0.1 810,675 + -0.1 810,675 + +0.6 810,675 + -1.1 810,675 + +1.0 810,676 - -1.2 810,676 - +0.7 810,676 - +0.3 810,676 - +0.6 810,717 + +1.1 810,717 + -0.3 810,717 + -1.1 810,717 + -0.6 810,717 + +0.4 810,717 + -0.4 810,717 + -0.2 810,717 + -1.3 810,717 + +0.2 810,718 - +1.2 810,718 - -0.1 810,718 - +0.3 810,718 - -1.5 810,718 - -0.1 810,833 + LRK54_RS03855 0.16 -1.2 810,833 + LRK54_RS03855 0.16 +0.1 810,833 + LRK54_RS03855 0.16 +0.2 810,833 + LRK54_RS03855 0.16 +1.0 810,833 + LRK54_RS03855 0.16 -0.1 810,833 + LRK54_RS03855 0.16 -0.5 810,833 + LRK54_RS03855 0.16 +0.2 810,833 + LRK54_RS03855 0.16 -0.4 810,833 + LRK54_RS03855 0.16 -1.0 810,833 + LRK54_RS03855 0.16 -0.2 810,833 + LRK54_RS03855 0.16 -0.4 810,834 - LRK54_RS03855 0.16 +0.0 810,834 - LRK54_RS03855 0.16 +0.2 810,834 - LRK54_RS03855 0.16 +0.1 810,834 - LRK54_RS03855 0.16 +1.6 810,834 - LRK54_RS03855 0.16 -2.3 810,834 - LRK54_RS03855 0.16 -0.1 810,834 - LRK54_RS03855 0.16 -0.9 810,834 - LRK54_RS03855 0.16 -0.8 810,834 - LRK54_RS03855 0.16 -0.4 810,834 - LRK54_RS03855 0.16 -0.5 810,834 - LRK54_RS03855 0.16 +1.3 810,834 - LRK54_RS03855 0.16 -0.5 810,842 + LRK54_RS03855 0.18 -1.5 810,842 + LRK54_RS03855 0.18 +1.2 810,923 + LRK54_RS03855 0.37 -0.4 810,923 + LRK54_RS03855 0.37 +1.1 810,923 + LRK54_RS03855 0.37 -0.4 810,923 + LRK54_RS03855 0.37 -0.0 810,923 + LRK54_RS03855 0.37 +0.8 810,923 + LRK54_RS03855 0.37 -0.6 810,923 + LRK54_RS03855 0.37 +2.0 810,923 + LRK54_RS03855 0.37 +0.3 810,923 + LRK54_RS03855 0.37 -1.2 810,923 + LRK54_RS03855 0.37 -0.2 810,923 + LRK54_RS03855 0.37 +0.6 810,923 + LRK54_RS03855 0.37 +0.2 810,924 - LRK54_RS03855 0.38 +0.3 810,924 - LRK54_RS03855 0.38 -0.7 810,924 - LRK54_RS03855 0.38 -0.1 810,924 - LRK54_RS03855 0.38 -0.6 810,924 - LRK54_RS03855 0.38 +1.3 810,924 - LRK54_RS03855 0.38 +0.3 811,211 + +1.3 811,212 - -0.4 811,420 + LRK54_RS03860 0.31 +0.4 811,420 + LRK54_RS03860 0.31 -0.6 811,420 + LRK54_RS03860 0.31 +0.3 811,420 + LRK54_RS03860 0.31 +0.2 811,421 - LRK54_RS03860 0.31 +0.5 811,421 - LRK54_RS03860 0.31 +1.0 811,506 + LRK54_RS03860 0.46 +1.3 811,506 + LRK54_RS03860 0.46 +0.6 811,506 + LRK54_RS03860 0.46 -2.0 811,507 - LRK54_RS03860 0.46 +1.1 811,507 - LRK54_RS03860 0.46 +1.6 811,507 - LRK54_RS03860 0.46 -0.2 811,626 + LRK54_RS03860 0.67 +0.1 811,626 + LRK54_RS03860 0.67 +0.3 811,626 + LRK54_RS03860 0.67 -1.0 811,626 + LRK54_RS03860 0.67 -0.1 811,627 - LRK54_RS03860 0.67 +1.3 811,627 - LRK54_RS03860 0.67 -0.2 811,671 + LRK54_RS03860 0.75 -0.1 811,671 + LRK54_RS03860 0.75 +0.9 811,760 - +0.8 811,865 + -2.4 811,865 + -0.4 811,865 + -0.3 811,865 + -0.8 811,865 + -0.4 811,865 + +0.3 811,865 + +1.2 811,865 + +0.8 811,865 + +0.4 811,865 + -0.5 811,866 - +0.4 811,866 - +0.5 811,866 - +0.3 811,866 - +0.6 811,866 - +0.3 811,866 - -1.1 811,866 - -0.2 811,866 - +0.2 811,943 + -0.0 811,943 + +0.6 812,042 + LRK54_RS03865 0.21 -0.0 812,042 + LRK54_RS03865 0.21 -0.3 812,043 - LRK54_RS03865 0.21 +2.3 812,051 + LRK54_RS03865 0.22 +1.0 812,051 + LRK54_RS03865 0.22 +0.6 812,051 + LRK54_RS03865 0.22 +0.0 812,051 + LRK54_RS03865 0.22 +2.0 812,052 - LRK54_RS03865 0.22 -0.0 812,052 - LRK54_RS03865 0.22 +0.9 812,052 - LRK54_RS03865 0.22 +0.3 812,052 - LRK54_RS03865 0.22 -0.4 812,052 - LRK54_RS03865 0.22 +0.1 812,052 - LRK54_RS03865 0.22 -0.1 812,052 - LRK54_RS03865 0.22 -0.6 812,282 + LRK54_RS03865 0.49 +2.3 812,282 + LRK54_RS03865 0.49 -0.5 812,283 - LRK54_RS03865 0.49 -1.1 812,283 - LRK54_RS03865 0.49 +0.4 812,283 - LRK54_RS03865 0.49 +0.4 812,357 + LRK54_RS03865 0.58 +0.4 812,357 + LRK54_RS03865 0.58 +0.8 812,357 + LRK54_RS03865 0.58 -0.4 812,358 - LRK54_RS03865 0.58 -2.5 812,358 - LRK54_RS03865 0.58 +0.7 812,358 - LRK54_RS03865 0.58 +0.2 812,367 + LRK54_RS03865 0.59 -1.2 812,367 + LRK54_RS03865 0.59 -0.4 812,368 - LRK54_RS03865 0.59 -0.5 812,368 - LRK54_RS03865 0.59 -0.0 812,368 - LRK54_RS03865 0.59 -1.3
Or see this region's nucleotide sequence