Experiment: R2A with FW104 filtrate 0.5 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS01260 and LRK54_RS01270 are separated by 225 nucleotides LRK54_RS01270 and LRK54_RS01275 are separated by 81 nucleotides LRK54_RS01275 and LRK54_RS01280 are separated by 97 nucleotides LRK54_RS01280 and LRK54_RS01285 overlap by 4 nucleotides LRK54_RS01285 and LRK54_RS01290 are separated by 34 nucleotides
LRK54_RS01260: LRK54_RS01260 - DNA polymerase III subunit gamma/tau, at 276,260 to 277,945
_RS01260
LRK54_RS01270: LRK54_RS01270 - tRNA-Ser, at 278,171 to 278,263
_RS01270
LRK54_RS01275: LRK54_RS01275 - tRNA-Ser, at 278,345 to 278,456
_RS01275
LRK54_RS01280: LRK54_RS01280 - MBL fold metallo-hydrolase, at 278,554 to 279,312
_RS01280
LRK54_RS01285: LRK54_RS01285 - 3-deoxy-D-manno-octulosonic acid kinase, at 279,309 to 280,040
_RS01285
LRK54_RS01290: LRK54_RS01290 - glycosyltransferase family 9 protein, at 280,075 to 281,118
_RS01290
Position (kb) 278
279
280 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 277.939 kb on - strand at 277.939 kb on - strand at 277.993 kb on + strand at 277.993 kb on + strand at 277.993 kb on + strand at 277.993 kb on + strand at 277.993 kb on + strand at 277.993 kb on + strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 277.994 kb on - strand at 278.001 kb on + strand at 278.001 kb on + strand at 278.002 kb on - strand at 278.275 kb on - strand at 278.406 kb on - strand, within LRK54_RS01275 at 278.406 kb on - strand, within LRK54_RS01275 at 278.406 kb on - strand, within LRK54_RS01275 at 278.413 kb on - strand, within LRK54_RS01275 at 278.413 kb on - strand, within LRK54_RS01275 at 278.413 kb on - strand, within LRK54_RS01275 at 278.413 kb on - strand, within LRK54_RS01275 at 278.413 kb on - strand, within LRK54_RS01275 at 278.413 kb on - strand, within LRK54_RS01275 at 278.413 kb on - strand, within LRK54_RS01275 at 278.413 kb on - strand, within LRK54_RS01275 at 278.413 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.415 kb on - strand, within LRK54_RS01275 at 278.494 kb on - strand at 278.494 kb on - strand at 278.494 kb on - strand at 278.494 kb on - strand at 278.530 kb on - strand at 278.530 kb on - strand at 278.530 kb on - strand at 278.530 kb on - strand at 278.666 kb on + strand, within LRK54_RS01280 at 278.672 kb on + strand, within LRK54_RS01280 at 278.672 kb on + strand, within LRK54_RS01280 at 278.673 kb on - strand, within LRK54_RS01280 at 278.673 kb on - strand, within LRK54_RS01280 at 278.673 kb on - strand, within LRK54_RS01280 at 278.706 kb on - strand, within LRK54_RS01280 at 278.878 kb on + strand, within LRK54_RS01280 at 278.878 kb on + strand, within LRK54_RS01280 at 278.879 kb on - strand, within LRK54_RS01280 at 279.179 kb on + strand, within LRK54_RS01280 at 279.299 kb on - strand at 279.323 kb on + strand at 279.323 kb on + strand at 279.323 kb on + strand at 279.323 kb on + strand at 279.324 kb on - strand at 279.324 kb on - strand at 279.324 kb on - strand at 279.337 kb on + strand at 279.338 kb on - strand at 279.338 kb on - strand at 279.490 kb on + strand, within LRK54_RS01285 at 279.490 kb on + strand, within LRK54_RS01285 at 279.490 kb on + strand, within LRK54_RS01285 at 279.491 kb on - strand, within LRK54_RS01285 at 279.491 kb on - strand, within LRK54_RS01285 at 279.491 kb on - strand, within LRK54_RS01285 at 279.674 kb on + strand, within LRK54_RS01285 at 279.674 kb on + strand, within LRK54_RS01285 at 279.676 kb on + strand, within LRK54_RS01285 at 279.676 kb on + strand, within LRK54_RS01285 at 279.676 kb on + strand, within LRK54_RS01285 at 279.676 kb on + strand, within LRK54_RS01285 at 279.676 kb on + strand, within LRK54_RS01285 at 279.676 kb on + strand, within LRK54_RS01285 at 279.676 kb on + strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.677 kb on - strand, within LRK54_RS01285 at 279.753 kb on + strand, within LRK54_RS01285 at 279.753 kb on + strand, within LRK54_RS01285 at 279.753 kb on + strand, within LRK54_RS01285 at 279.753 kb on + strand, within LRK54_RS01285 at 279.753 kb on + strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.754 kb on - strand, within LRK54_RS01285 at 279.802 kb on + strand, within LRK54_RS01285 at 279.845 kb on - strand, within LRK54_RS01285 at 279.940 kb on + strand, within LRK54_RS01285 at 279.940 kb on + strand, within LRK54_RS01285 at 279.941 kb on - strand, within LRK54_RS01285 at 279.978 kb on + strand at 279.979 kb on - strand at 279.979 kb on - strand at 280.063 kb on + strand at 280.063 kb on + strand at 280.064 kb on - strand at 280.064 kb on - strand at 280.064 kb on - strand at 280.064 kb on - strand at 280.064 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with FW104 filtrate 0.5 X remove 277,939 - -0.0 277,939 - +0.6 277,993 + +2.3 277,993 + -0.7 277,993 + -0.2 277,993 + -0.0 277,993 + -0.2 277,993 + +0.7 277,994 - -0.3 277,994 - +0.4 277,994 - -2.0 277,994 - -1.0 277,994 - -1.1 277,994 - -0.2 277,994 - -0.1 277,994 - +0.4 277,994 - -0.7 277,994 - -0.7 277,994 - +1.6 277,994 - -0.4 277,994 - -1.1 277,994 - +0.3 277,994 - +0.1 277,994 - -0.4 278,001 + -1.0 278,001 + -0.2 278,002 - -0.6 278,275 - +0.2 278,406 - LRK54_RS01275 0.54 -0.3 278,406 - LRK54_RS01275 0.54 -0.7 278,406 - LRK54_RS01275 0.54 +2.3 278,413 - LRK54_RS01275 0.61 +0.3 278,413 - LRK54_RS01275 0.61 +0.4 278,413 - LRK54_RS01275 0.61 +1.6 278,413 - LRK54_RS01275 0.61 -1.9 278,413 - LRK54_RS01275 0.61 -0.2 278,413 - LRK54_RS01275 0.61 -0.1 278,413 - LRK54_RS01275 0.61 +0.7 278,413 - LRK54_RS01275 0.61 -1.2 278,413 - LRK54_RS01275 0.61 -0.5 278,415 - LRK54_RS01275 0.62 +1.7 278,415 - LRK54_RS01275 0.62 -0.5 278,415 - LRK54_RS01275 0.62 +0.7 278,415 - LRK54_RS01275 0.62 -0.3 278,415 - LRK54_RS01275 0.62 -0.0 278,415 - LRK54_RS01275 0.62 -0.4 278,415 - LRK54_RS01275 0.62 +0.4 278,415 - LRK54_RS01275 0.62 +0.2 278,415 - LRK54_RS01275 0.62 +1.9 278,415 - LRK54_RS01275 0.62 -0.2 278,415 - LRK54_RS01275 0.62 -0.5 278,415 - LRK54_RS01275 0.62 +0.9 278,415 - LRK54_RS01275 0.62 +0.0 278,415 - LRK54_RS01275 0.62 -1.3 278,415 - LRK54_RS01275 0.62 +0.3 278,415 - LRK54_RS01275 0.62 +0.5 278,415 - LRK54_RS01275 0.62 -0.5 278,415 - LRK54_RS01275 0.62 -1.2 278,415 - LRK54_RS01275 0.62 +1.0 278,415 - LRK54_RS01275 0.62 -2.8 278,415 - LRK54_RS01275 0.62 +1.4 278,415 - LRK54_RS01275 0.62 +0.3 278,415 - LRK54_RS01275 0.62 +0.0 278,415 - LRK54_RS01275 0.62 -0.3 278,415 - LRK54_RS01275 0.62 +1.2 278,415 - LRK54_RS01275 0.62 -1.0 278,494 - -0.0 278,494 - -0.4 278,494 - -0.4 278,494 - +0.5 278,530 - -0.9 278,530 - -0.3 278,530 - +0.6 278,530 - +4.6 278,666 + LRK54_RS01280 0.15 -0.3 278,672 + LRK54_RS01280 0.16 +0.3 278,672 + LRK54_RS01280 0.16 -0.5 278,673 - LRK54_RS01280 0.16 +0.3 278,673 - LRK54_RS01280 0.16 -0.5 278,673 - LRK54_RS01280 0.16 -0.5 278,706 - LRK54_RS01280 0.20 -0.7 278,878 + LRK54_RS01280 0.43 -0.3 278,878 + LRK54_RS01280 0.43 -1.2 278,879 - LRK54_RS01280 0.43 -0.9 279,179 + LRK54_RS01280 0.82 +1.3 279,299 - +3.3 279,323 + -1.9 279,323 + -0.8 279,323 + -0.7 279,323 + +0.7 279,324 - -0.3 279,324 - +2.5 279,324 - -0.2 279,337 + -1.3 279,338 - +0.3 279,338 - +0.3 279,490 + LRK54_RS01285 0.25 +0.5 279,490 + LRK54_RS01285 0.25 +1.9 279,490 + LRK54_RS01285 0.25 -1.2 279,491 - LRK54_RS01285 0.25 -0.7 279,491 - LRK54_RS01285 0.25 +0.7 279,491 - LRK54_RS01285 0.25 +0.2 279,674 + LRK54_RS01285 0.50 +0.1 279,674 + LRK54_RS01285 0.50 -0.7 279,676 + LRK54_RS01285 0.50 -1.2 279,676 + LRK54_RS01285 0.50 +1.0 279,676 + LRK54_RS01285 0.50 -0.3 279,676 + LRK54_RS01285 0.50 -0.7 279,676 + LRK54_RS01285 0.50 +0.3 279,676 + LRK54_RS01285 0.50 -0.0 279,676 + LRK54_RS01285 0.50 +0.3 279,677 - LRK54_RS01285 0.50 +0.7 279,677 - LRK54_RS01285 0.50 -2.5 279,677 - LRK54_RS01285 0.50 -1.4 279,677 - LRK54_RS01285 0.50 -0.7 279,677 - LRK54_RS01285 0.50 -0.7 279,677 - LRK54_RS01285 0.50 +1.0 279,677 - LRK54_RS01285 0.50 -0.4 279,677 - LRK54_RS01285 0.50 -0.6 279,677 - LRK54_RS01285 0.50 -0.7 279,677 - LRK54_RS01285 0.50 -0.0 279,677 - LRK54_RS01285 0.50 +0.7 279,677 - LRK54_RS01285 0.50 -0.3 279,677 - LRK54_RS01285 0.50 -0.9 279,677 - LRK54_RS01285 0.50 +0.9 279,677 - LRK54_RS01285 0.50 -0.9 279,753 + LRK54_RS01285 0.61 -0.0 279,753 + LRK54_RS01285 0.61 +0.7 279,753 + LRK54_RS01285 0.61 -0.3 279,753 + LRK54_RS01285 0.61 -0.0 279,753 + LRK54_RS01285 0.61 +1.1 279,754 - LRK54_RS01285 0.61 +0.3 279,754 - LRK54_RS01285 0.61 -1.2 279,754 - LRK54_RS01285 0.61 -0.3 279,754 - LRK54_RS01285 0.61 -0.0 279,754 - LRK54_RS01285 0.61 -0.7 279,754 - LRK54_RS01285 0.61 -0.3 279,754 - LRK54_RS01285 0.61 -1.3 279,754 - LRK54_RS01285 0.61 +1.3 279,754 - LRK54_RS01285 0.61 -0.9 279,754 - LRK54_RS01285 0.61 +3.5 279,754 - LRK54_RS01285 0.61 -0.9 279,802 + LRK54_RS01285 0.67 -0.5 279,845 - LRK54_RS01285 0.73 -0.9 279,940 + LRK54_RS01285 0.86 -2.1 279,940 + LRK54_RS01285 0.86 -1.2 279,941 - LRK54_RS01285 0.86 +1.9 279,978 + -1.4 279,979 - +0.5 279,979 - -0.7 280,063 + -0.0 280,063 + +1.0 280,064 - -2.2 280,064 - +0.6 280,064 - +0.5 280,064 - -1.0 280,064 - -1.0
Or see this region's nucleotide sequence