Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS15040 and HSERO_RS15045 are separated by 57 nucleotides HSERO_RS15045 and HSERO_RS15050 are separated by 152 nucleotides HSERO_RS15050 and HSERO_RS15055 overlap by 4 nucleotides HSERO_RS15055 and HSERO_RS15060 are separated by 199 nucleotides
HSERO_RS15040: HSERO_RS15040 - poly(R)-hydroxyalkanoic acid synthase, at 3,413,862 to 3,415,685
_RS15040
HSERO_RS15045: HSERO_RS15045 - membrane protein, at 3,415,743 to 3,416,012
_RS15045
HSERO_RS15050: HSERO_RS15050 - hypothetical protein, at 3,416,165 to 3,416,425
_RS15050
HSERO_RS15055: HSERO_RS15055 - membrane protein, at 3,416,422 to 3,417,204
_RS15055
HSERO_RS15060: HSERO_RS15060 - LysR family transcriptional regulator, at 3,417,404 to 3,418,297
_RS15060
Position (kb)
3416
3417
3418 Strain fitness (log2 ratio)
-1
0
1 at 3415.422 kb on - strand, within HSERO_RS15040 at 3415.422 kb on - strand, within HSERO_RS15040 at 3415.549 kb on + strand at 3415.604 kb on - strand at 3415.606 kb on + strand at 3415.606 kb on + strand at 3415.718 kb on - strand at 3415.802 kb on + strand, within HSERO_RS15045 at 3415.809 kb on + strand, within HSERO_RS15045 at 3415.843 kb on - strand, within HSERO_RS15045 at 3415.907 kb on - strand, within HSERO_RS15045 at 3416.011 kb on + strand at 3416.023 kb on + strand at 3416.033 kb on + strand at 3416.041 kb on - strand at 3416.343 kb on - strand, within HSERO_RS15050 at 3416.351 kb on + strand, within HSERO_RS15050 at 3416.353 kb on - strand, within HSERO_RS15050 at 3416.359 kb on - strand, within HSERO_RS15050 at 3416.585 kb on + strand, within HSERO_RS15055 at 3417.102 kb on - strand, within HSERO_RS15055 at 3417.255 kb on - strand at 3417.319 kb on - strand at 3417.323 kb on + strand at 3417.448 kb on - strand at 3417.578 kb on + strand, within HSERO_RS15060 at 3417.701 kb on - strand, within HSERO_RS15060 at 3417.703 kb on + strand, within HSERO_RS15060 at 3417.863 kb on + strand, within HSERO_RS15060 at 3417.878 kb on + strand, within HSERO_RS15060 at 3418.120 kb on + strand, within HSERO_RS15060 at 3418.143 kb on - strand, within HSERO_RS15060
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 3,415,422 - HSERO_RS15040 0.86 +0.5 3,415,422 - HSERO_RS15040 0.86 +0.5 3,415,549 + +0.8 3,415,604 - +0.2 3,415,606 + +0.3 3,415,606 + +1.3 3,415,718 - +0.6 3,415,802 + HSERO_RS15045 0.22 +0.3 3,415,809 + HSERO_RS15045 0.24 +0.1 3,415,843 - HSERO_RS15045 0.37 -1.0 3,415,907 - HSERO_RS15045 0.61 +0.6 3,416,011 + +0.2 3,416,023 + +0.1 3,416,033 + +0.3 3,416,041 - +0.4 3,416,343 - HSERO_RS15050 0.68 -0.1 3,416,351 + HSERO_RS15050 0.71 +0.2 3,416,353 - HSERO_RS15050 0.72 -0.8 3,416,359 - HSERO_RS15050 0.74 -0.1 3,416,585 + HSERO_RS15055 0.21 -0.6 3,417,102 - HSERO_RS15055 0.87 +1.5 3,417,255 - -0.2 3,417,319 - -0.2 3,417,323 + +0.5 3,417,448 - +1.4 3,417,578 + HSERO_RS15060 0.19 -0.7 3,417,701 - HSERO_RS15060 0.33 +0.0 3,417,703 + HSERO_RS15060 0.33 +0.1 3,417,863 + HSERO_RS15060 0.51 +0.5 3,417,878 + HSERO_RS15060 0.53 +0.8 3,418,120 + HSERO_RS15060 0.80 +0.2 3,418,143 - HSERO_RS15060 0.83 -0.1
Or see this region's nucleotide sequence