Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01915 and HSERO_RS01920 are separated by 3 nucleotides HSERO_RS01920 and HSERO_RS01925 are separated by 41 nucleotides HSERO_RS01925 and HSERO_RS01930 are separated by 95 nucleotides HSERO_RS01930 and HSERO_RS01935 are separated by 134 nucleotides
HSERO_RS01915: HSERO_RS01915 - membrane protein, at 421,059 to 422,348
_RS01915
HSERO_RS01920: HSERO_RS01920 - 50S ribosomal protein L11 methyltransferase, at 422,352 to 423,266
_RS01920
HSERO_RS01925: HSERO_RS01925 - acetyl-CoA carboxylase biotin carboxylase subunit, at 423,308 to 424,687
_RS01925
HSERO_RS01930: HSERO_RS01930 - acetyl-CoA carboxylase, at 424,783 to 425,256
_RS01930
HSERO_RS01935: HSERO_RS01935 - 3-dehydroquinate dehydratase, at 425,391 to 425,828
_RS01935
Position (kb)
423
424
425 Strain fitness (log2 ratio)
-2
-1
0
1 at 422.413 kb on - strand at 422.510 kb on + strand, within HSERO_RS01920 at 422.518 kb on - strand, within HSERO_RS01920 at 422.609 kb on + strand, within HSERO_RS01920 at 422.751 kb on + strand, within HSERO_RS01920 at 422.972 kb on - strand, within HSERO_RS01920 at 423.001 kb on + strand, within HSERO_RS01920 at 423.014 kb on - strand, within HSERO_RS01920 at 425.397 kb on - strand at 425.456 kb on - strand, within HSERO_RS01935 at 425.573 kb on - strand, within HSERO_RS01935
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 422,413 - -0.1 422,510 + HSERO_RS01920 0.17 +0.9 422,518 - HSERO_RS01920 0.18 +0.3 422,609 + HSERO_RS01920 0.28 +0.3 422,751 + HSERO_RS01920 0.44 -0.1 422,972 - HSERO_RS01920 0.68 +1.3 423,001 + HSERO_RS01920 0.71 +0.0 423,014 - HSERO_RS01920 0.72 +1.3 425,397 - -2.2 425,456 - HSERO_RS01935 0.15 -0.1 425,573 - HSERO_RS01935 0.42 -0.1
Or see this region's nucleotide sequence