Experiment: CDM1 without Cyanocobalamin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16600 and BBR_RS16605 are separated by 118 nucleotides BBR_RS16605 and BBR_RS16610 are separated by 64 nucleotides BBR_RS16610 and BBR_RS16615 are separated by 11 nucleotides
BBR_RS16600: BBR_RS16600 - aconitate hydratase AcnA, at 1,490,388 to 1,493,087
_RS16600
BBR_RS16605: BBR_RS16605 - hypothetical protein, at 1,493,206 to 1,495,818
_RS16605
BBR_RS16610: BBR_RS16610 - hypothetical protein, at 1,495,883 to 1,496,467
_RS16610
BBR_RS16615: BBR_RS16615 - class I SAM-dependent RNA methyltransferase, at 1,496,479 to 1,497,738
_RS16615
Position (kb)
1493
1494
1495
1496 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1492.293 kb on + strand, within BBR_RS16600 at 1492.293 kb on + strand, within BBR_RS16600 at 1492.294 kb on + strand, within BBR_RS16600 at 1492.351 kb on - strand, within BBR_RS16600 at 1492.429 kb on - strand, within BBR_RS16600 at 1492.461 kb on - strand, within BBR_RS16600 at 1492.463 kb on - strand, within BBR_RS16600 at 1492.636 kb on - strand, within BBR_RS16600 at 1492.706 kb on - strand, within BBR_RS16600 at 1492.715 kb on - strand, within BBR_RS16600 at 1492.730 kb on - strand, within BBR_RS16600 at 1492.746 kb on - strand, within BBR_RS16600 at 1492.768 kb on - strand, within BBR_RS16600 at 1492.787 kb on - strand, within BBR_RS16600 at 1492.821 kb on + strand at 1492.890 kb on + strand at 1492.912 kb on + strand at 1492.912 kb on + strand at 1492.912 kb on + strand at 1492.912 kb on + strand at 1492.914 kb on - strand at 1492.914 kb on - strand at 1492.919 kb on + strand at 1492.922 kb on + strand at 1492.924 kb on + strand at 1493.040 kb on - strand at 1493.085 kb on - strand at 1493.149 kb on + strand at 1493.149 kb on + strand at 1493.276 kb on - strand at 1493.295 kb on - strand at 1493.385 kb on + strand at 1493.429 kb on - strand at 1493.523 kb on - strand, within BBR_RS16605 at 1493.706 kb on - strand, within BBR_RS16605 at 1493.706 kb on - strand, within BBR_RS16605 at 1493.735 kb on + strand, within BBR_RS16605 at 1493.748 kb on + strand, within BBR_RS16605 at 1493.760 kb on + strand, within BBR_RS16605 at 1493.760 kb on + strand, within BBR_RS16605 at 1493.760 kb on + strand, within BBR_RS16605 at 1493.768 kb on - strand, within BBR_RS16605 at 1493.786 kb on + strand, within BBR_RS16605 at 1493.794 kb on - strand, within BBR_RS16605 at 1493.796 kb on - strand, within BBR_RS16605 at 1493.839 kb on + strand, within BBR_RS16605 at 1493.901 kb on - strand, within BBR_RS16605 at 1493.930 kb on + strand, within BBR_RS16605 at 1493.943 kb on - strand, within BBR_RS16605 at 1494.004 kb on - strand, within BBR_RS16605 at 1494.012 kb on + strand, within BBR_RS16605 at 1494.063 kb on - strand, within BBR_RS16605 at 1494.063 kb on - strand, within BBR_RS16605 at 1494.063 kb on - strand, within BBR_RS16605 at 1494.068 kb on - strand, within BBR_RS16605 at 1494.075 kb on - strand, within BBR_RS16605 at 1494.226 kb on + strand, within BBR_RS16605 at 1494.234 kb on - strand, within BBR_RS16605 at 1494.290 kb on - strand, within BBR_RS16605 at 1494.356 kb on + strand, within BBR_RS16605 at 1494.403 kb on + strand, within BBR_RS16605 at 1494.459 kb on + strand, within BBR_RS16605 at 1494.475 kb on + strand, within BBR_RS16605 at 1494.478 kb on - strand, within BBR_RS16605 at 1494.578 kb on + strand, within BBR_RS16605 at 1494.584 kb on + strand, within BBR_RS16605 at 1494.585 kb on + strand, within BBR_RS16605 at 1494.623 kb on - strand, within BBR_RS16605 at 1494.654 kb on + strand, within BBR_RS16605 at 1494.693 kb on + strand, within BBR_RS16605 at 1494.701 kb on - strand, within BBR_RS16605 at 1494.727 kb on + strand, within BBR_RS16605 at 1494.727 kb on + strand, within BBR_RS16605 at 1494.731 kb on + strand, within BBR_RS16605 at 1494.737 kb on - strand, within BBR_RS16605 at 1494.842 kb on - strand, within BBR_RS16605 at 1494.931 kb on - strand, within BBR_RS16605 at 1494.980 kb on + strand, within BBR_RS16605 at 1495.099 kb on - strand, within BBR_RS16605 at 1495.120 kb on + strand, within BBR_RS16605 at 1495.126 kb on - strand, within BBR_RS16605 at 1495.126 kb on - strand, within BBR_RS16605 at 1495.150 kb on - strand, within BBR_RS16605 at 1495.158 kb on - strand, within BBR_RS16605 at 1495.168 kb on + strand, within BBR_RS16605 at 1495.177 kb on - strand, within BBR_RS16605 at 1495.202 kb on + strand, within BBR_RS16605 at 1495.210 kb on - strand, within BBR_RS16605 at 1495.244 kb on + strand, within BBR_RS16605 at 1495.250 kb on + strand, within BBR_RS16605 at 1495.258 kb on - strand, within BBR_RS16605 at 1495.370 kb on + strand, within BBR_RS16605 at 1495.383 kb on - strand, within BBR_RS16605 at 1495.384 kb on - strand, within BBR_RS16605 at 1495.517 kb on + strand, within BBR_RS16605 at 1495.538 kb on + strand, within BBR_RS16605 at 1495.538 kb on + strand, within BBR_RS16605 at 1495.538 kb on + strand, within BBR_RS16605 at 1495.546 kb on - strand, within BBR_RS16605 at 1495.660 kb on - strand at 1495.687 kb on + strand at 1495.704 kb on + strand at 1495.772 kb on - strand at 1495.816 kb on + strand at 1495.819 kb on - strand at 1495.910 kb on + strand at 1495.910 kb on + strand at 1495.962 kb on + strand, within BBR_RS16610 at 1496.003 kb on + strand, within BBR_RS16610 at 1496.009 kb on - strand, within BBR_RS16610 at 1496.023 kb on - strand, within BBR_RS16610 at 1496.053 kb on - strand, within BBR_RS16610 at 1496.075 kb on + strand, within BBR_RS16610 at 1496.086 kb on + strand, within BBR_RS16610 at 1496.104 kb on - strand, within BBR_RS16610 at 1496.235 kb on + strand, within BBR_RS16610 at 1496.253 kb on - strand, within BBR_RS16610 at 1496.377 kb on - strand, within BBR_RS16610 at 1496.412 kb on + strand at 1496.472 kb on - strand at 1496.475 kb on + strand at 1496.561 kb on - strand at 1496.565 kb on + strand at 1496.569 kb on + strand at 1496.623 kb on - strand, within BBR_RS16615 at 1496.689 kb on + strand, within BBR_RS16615 at 1496.719 kb on + strand, within BBR_RS16615 at 1496.719 kb on + strand, within BBR_RS16615 at 1496.741 kb on + strand, within BBR_RS16615 at 1496.741 kb on + strand, within BBR_RS16615 at 1496.741 kb on + strand, within BBR_RS16615 at 1496.749 kb on - strand, within BBR_RS16615 at 1496.749 kb on - strand, within BBR_RS16615 at 1496.759 kb on - strand, within BBR_RS16615
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Cyanocobalamin remove 1,492,293 + BBR_RS16600 0.71 -2.7 1,492,293 + BBR_RS16600 0.71 -2.0 1,492,294 + BBR_RS16600 0.71 -3.0 1,492,351 - BBR_RS16600 0.73 -0.6 1,492,429 - BBR_RS16600 0.76 -3.4 1,492,461 - BBR_RS16600 0.77 -3.5 1,492,463 - BBR_RS16600 0.77 -2.4 1,492,636 - BBR_RS16600 0.83 -1.0 1,492,706 - BBR_RS16600 0.86 -1.9 1,492,715 - BBR_RS16600 0.86 -3.1 1,492,730 - BBR_RS16600 0.87 -2.5 1,492,746 - BBR_RS16600 0.87 -3.2 1,492,768 - BBR_RS16600 0.88 -1.9 1,492,787 - BBR_RS16600 0.89 -2.8 1,492,821 + -2.4 1,492,890 + -2.2 1,492,912 + -2.1 1,492,912 + -0.9 1,492,912 + -0.3 1,492,912 + -3.2 1,492,914 - -2.4 1,492,914 - -1.6 1,492,919 + -4.1 1,492,922 + -3.1 1,492,924 + -3.7 1,493,040 - -3.4 1,493,085 - -0.9 1,493,149 + -1.4 1,493,149 + -1.0 1,493,276 - +1.1 1,493,295 - +1.9 1,493,385 + -0.8 1,493,429 - +0.1 1,493,523 - BBR_RS16605 0.12 -3.3 1,493,706 - BBR_RS16605 0.19 -1.9 1,493,706 - BBR_RS16605 0.19 -2.0 1,493,735 + BBR_RS16605 0.20 -1.2 1,493,748 + BBR_RS16605 0.21 -2.7 1,493,760 + BBR_RS16605 0.21 -2.1 1,493,760 + BBR_RS16605 0.21 +0.1 1,493,760 + BBR_RS16605 0.21 -0.7 1,493,768 - BBR_RS16605 0.22 -2.0 1,493,786 + BBR_RS16605 0.22 -2.6 1,493,794 - BBR_RS16605 0.23 -0.8 1,493,796 - BBR_RS16605 0.23 -3.0 1,493,839 + BBR_RS16605 0.24 -0.9 1,493,901 - BBR_RS16605 0.27 -0.3 1,493,930 + BBR_RS16605 0.28 +0.1 1,493,943 - BBR_RS16605 0.28 -0.1 1,494,004 - BBR_RS16605 0.31 -1.8 1,494,012 + BBR_RS16605 0.31 -1.1 1,494,063 - BBR_RS16605 0.33 -1.8 1,494,063 - BBR_RS16605 0.33 -1.5 1,494,063 - BBR_RS16605 0.33 -2.6 1,494,068 - BBR_RS16605 0.33 +0.1 1,494,075 - BBR_RS16605 0.33 -3.4 1,494,226 + BBR_RS16605 0.39 -0.9 1,494,234 - BBR_RS16605 0.39 -3.1 1,494,290 - BBR_RS16605 0.41 -1.6 1,494,356 + BBR_RS16605 0.44 -2.3 1,494,403 + BBR_RS16605 0.46 -2.4 1,494,459 + BBR_RS16605 0.48 -1.3 1,494,475 + BBR_RS16605 0.49 -1.7 1,494,478 - BBR_RS16605 0.49 -1.8 1,494,578 + BBR_RS16605 0.53 -0.6 1,494,584 + BBR_RS16605 0.53 -1.2 1,494,585 + BBR_RS16605 0.53 +0.4 1,494,623 - BBR_RS16605 0.54 -2.8 1,494,654 + BBR_RS16605 0.55 -0.5 1,494,693 + BBR_RS16605 0.57 -3.3 1,494,701 - BBR_RS16605 0.57 -1.4 1,494,727 + BBR_RS16605 0.58 -2.3 1,494,727 + BBR_RS16605 0.58 -1.7 1,494,731 + BBR_RS16605 0.58 -2.3 1,494,737 - BBR_RS16605 0.59 -0.8 1,494,842 - BBR_RS16605 0.63 -1.1 1,494,931 - BBR_RS16605 0.66 -2.3 1,494,980 + BBR_RS16605 0.68 -1.7 1,495,099 - BBR_RS16605 0.72 -3.4 1,495,120 + BBR_RS16605 0.73 -1.5 1,495,126 - BBR_RS16605 0.73 -2.3 1,495,126 - BBR_RS16605 0.73 -2.5 1,495,150 - BBR_RS16605 0.74 -1.6 1,495,158 - BBR_RS16605 0.75 -2.9 1,495,168 + BBR_RS16605 0.75 -1.5 1,495,177 - BBR_RS16605 0.75 -1.8 1,495,202 + BBR_RS16605 0.76 -1.3 1,495,210 - BBR_RS16605 0.77 -2.7 1,495,244 + BBR_RS16605 0.78 -2.3 1,495,250 + BBR_RS16605 0.78 -0.8 1,495,258 - BBR_RS16605 0.79 -2.1 1,495,370 + BBR_RS16605 0.83 +0.1 1,495,383 - BBR_RS16605 0.83 -0.1 1,495,384 - BBR_RS16605 0.83 +0.1 1,495,517 + BBR_RS16605 0.88 -2.0 1,495,538 + BBR_RS16605 0.89 -1.3 1,495,538 + BBR_RS16605 0.89 -1.2 1,495,538 + BBR_RS16605 0.89 -0.3 1,495,546 - BBR_RS16605 0.90 +0.1 1,495,660 - -1.1 1,495,687 + -0.5 1,495,704 + -1.9 1,495,772 - -1.7 1,495,816 + -0.5 1,495,819 - -0.2 1,495,910 + -2.1 1,495,910 + -3.2 1,495,962 + BBR_RS16610 0.14 -2.9 1,496,003 + BBR_RS16610 0.21 -2.7 1,496,009 - BBR_RS16610 0.22 -1.7 1,496,023 - BBR_RS16610 0.24 -2.7 1,496,053 - BBR_RS16610 0.29 -1.5 1,496,075 + BBR_RS16610 0.33 -3.2 1,496,086 + BBR_RS16610 0.35 -4.2 1,496,104 - BBR_RS16610 0.38 -2.0 1,496,235 + BBR_RS16610 0.60 -2.2 1,496,253 - BBR_RS16610 0.63 -1.3 1,496,377 - BBR_RS16610 0.84 -1.7 1,496,412 + -3.0 1,496,472 - +0.2 1,496,475 + -0.5 1,496,561 - +2.1 1,496,565 + -0.2 1,496,569 + -1.2 1,496,623 - BBR_RS16615 0.11 +1.0 1,496,689 + BBR_RS16615 0.17 -3.0 1,496,719 + BBR_RS16615 0.19 -1.3 1,496,719 + BBR_RS16615 0.19 -1.7 1,496,741 + BBR_RS16615 0.21 -0.6 1,496,741 + BBR_RS16615 0.21 -1.6 1,496,741 + BBR_RS16615 0.21 -1.5 1,496,749 - BBR_RS16615 0.21 +1.7 1,496,749 - BBR_RS16615 0.21 -0.3 1,496,759 - BBR_RS16615 0.22 +1.1
Or see this region's nucleotide sequence