Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS15440

Experiment: CDM1 without Cyanocobalamin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS15435 and BBR_RS15440 are separated by 17 nucleotidesBBR_RS15440 and BBR_RS15445 are separated by 151 nucleotidesBBR_RS15445 and BBR_RS15450 are separated by 2 nucleotides BBR_RS15435: BBR_RS15435 - dihydroorotase, at 1,234,140 to 1,235,654 _RS15435 BBR_RS15440: BBR_RS15440 - orotidine-5'-phosphate decarboxylase, at 1,235,672 to 1,236,625 _RS15440 BBR_RS15445: BBR_RS15445 - dihydroorotate dehydrogenase electron transfer subunit, at 1,236,777 to 1,237,601 _RS15445 BBR_RS15450: BBR_RS15450 - dihydroorotate dehydrogenase, at 1,237,604 to 1,238,575 _RS15450 Position (kb) 1235 1236 1237Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1234.923 kb on + strand, within BBR_RS15435at 1235.122 kb on + strand, within BBR_RS15435at 1235.130 kb on + strand, within BBR_RS15435at 1235.130 kb on - strand, within BBR_RS15435at 1235.285 kb on + strand, within BBR_RS15435at 1235.330 kb on - strand, within BBR_RS15435at 1235.478 kb on + strand, within BBR_RS15435at 1235.486 kb on - strand, within BBR_RS15435at 1235.521 kb on + strandat 1235.560 kb on - strandat 1235.656 kb on + strandat 1235.658 kb on + strandat 1235.910 kb on + strand, within BBR_RS15440at 1235.913 kb on + strand, within BBR_RS15440at 1235.944 kb on + strand, within BBR_RS15440at 1235.944 kb on + strand, within BBR_RS15440at 1235.946 kb on + strand, within BBR_RS15440at 1235.989 kb on - strand, within BBR_RS15440at 1236.001 kb on + strand, within BBR_RS15440at 1236.042 kb on + strand, within BBR_RS15440at 1236.131 kb on - strand, within BBR_RS15440at 1236.186 kb on + strand, within BBR_RS15440at 1236.281 kb on + strand, within BBR_RS15440at 1236.294 kb on - strand, within BBR_RS15440at 1236.379 kb on + strand, within BBR_RS15440at 1236.454 kb on + strand, within BBR_RS15440at 1236.653 kb on + strandat 1236.796 kb on + strandat 1236.917 kb on - strand, within BBR_RS15445at 1236.923 kb on + strand, within BBR_RS15445at 1236.937 kb on - strand, within BBR_RS15445at 1236.956 kb on + strand, within BBR_RS15445at 1237.007 kb on + strand, within BBR_RS15445at 1237.007 kb on + strand, within BBR_RS15445at 1237.030 kb on - strand, within BBR_RS15445at 1237.062 kb on - strand, within BBR_RS15445at 1237.187 kb on - strand, within BBR_RS15445at 1237.192 kb on - strand, within BBR_RS15445at 1237.192 kb on - strand, within BBR_RS15445at 1237.194 kb on + strand, within BBR_RS15445at 1237.281 kb on - strand, within BBR_RS15445at 1237.308 kb on + strand, within BBR_RS15445at 1237.405 kb on - strand, within BBR_RS15445at 1237.426 kb on - strand, within BBR_RS15445at 1237.426 kb on - strand, within BBR_RS15445at 1237.496 kb on - strand, within BBR_RS15445at 1237.529 kb on - strandat 1237.573 kb on + strandat 1237.596 kb on - strandat 1237.606 kb on - strandat 1237.623 kb on - strandat 1237.625 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction CDM1 without Cyanocobalamin
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1,234,923 + BBR_RS15435 0.52 -0.2
1,235,122 + BBR_RS15435 0.65 -0.1
1,235,130 + BBR_RS15435 0.65 -1.0
1,235,130 - BBR_RS15435 0.65 +0.6
1,235,285 + BBR_RS15435 0.76 +0.3
1,235,330 - BBR_RS15435 0.79 +2.3
1,235,478 + BBR_RS15435 0.88 -1.7
1,235,486 - BBR_RS15435 0.89 -0.5
1,235,521 + +1.0
1,235,560 - +0.1
1,235,656 + +0.4
1,235,658 + +1.7
1,235,910 + BBR_RS15440 0.25 -2.4
1,235,913 + BBR_RS15440 0.25 +0.6
1,235,944 + BBR_RS15440 0.29 -0.2
1,235,944 + BBR_RS15440 0.29 -1.7
1,235,946 + BBR_RS15440 0.29 +0.6
1,235,989 - BBR_RS15440 0.33 +0.1
1,236,001 + BBR_RS15440 0.34 +1.9
1,236,042 + BBR_RS15440 0.39 -1.3
1,236,131 - BBR_RS15440 0.48 -0.4
1,236,186 + BBR_RS15440 0.54 -0.2
1,236,281 + BBR_RS15440 0.64 -1.8
1,236,294 - BBR_RS15440 0.65 -1.1
1,236,379 + BBR_RS15440 0.74 -0.1
1,236,454 + BBR_RS15440 0.82 -0.2
1,236,653 + +0.9
1,236,796 + +2.4
1,236,917 - BBR_RS15445 0.17 +3.1
1,236,923 + BBR_RS15445 0.18 +3.8
1,236,937 - BBR_RS15445 0.19 +3.8
1,236,956 + BBR_RS15445 0.22 +3.8
1,237,007 + BBR_RS15445 0.28 +1.9
1,237,007 + BBR_RS15445 0.28 +2.2
1,237,030 - BBR_RS15445 0.31 +1.8
1,237,062 - BBR_RS15445 0.35 +3.1
1,237,187 - BBR_RS15445 0.50 +2.9
1,237,192 - BBR_RS15445 0.50 +3.1
1,237,192 - BBR_RS15445 0.50 +2.9
1,237,194 + BBR_RS15445 0.51 +3.1
1,237,281 - BBR_RS15445 0.61 +3.3
1,237,308 + BBR_RS15445 0.64 +3.7
1,237,405 - BBR_RS15445 0.76 +3.2
1,237,426 - BBR_RS15445 0.79 +3.1
1,237,426 - BBR_RS15445 0.79 +2.7
1,237,496 - BBR_RS15445 0.87 +2.8
1,237,529 - +3.0
1,237,573 + +3.3
1,237,596 - -0.8
1,237,606 - +0.6
1,237,623 - +3.2
1,237,625 + +3.1

Or see this region's nucleotide sequence