Experiment: CDM1 without Cyanocobalamin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15085 and BBR_RS15090 are separated by 151 nucleotides BBR_RS15090 and BBR_RS20880 are separated by 59 nucleotides BBR_RS20880 and BBR_RS15095 overlap by 36 nucleotides BBR_RS15095 and BBR_RS15100 are separated by 160 nucleotides
BBR_RS15085: BBR_RS15085 - 3-dehydroquinate synthase, at 1,152,542 to 1,154,164
_RS15085
BBR_RS15090: BBR_RS15090 - type II 3-dehydroquinate dehydratase, at 1,154,316 to 1,154,762
_RS15090
BBR_RS20880: BBR_RS20880 - CTP synthetase, at 1,154,822 to 1,154,917
_RS20880
BBR_RS15095: BBR_RS15095 - CTP synthase, at 1,154,882 to 1,156,546
_RS15095
BBR_RS15100: BBR_RS15100 - murein L,D-transpeptidase, at 1,156,707 to 1,158,335
_RS15100
Position (kb)
1154
1155
1156
1157 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1154.191 kb on + strand at 1154.193 kb on + strand at 1154.201 kb on - strand at 1154.219 kb on - strand at 1154.228 kb on - strand at 1154.229 kb on - strand at 1154.239 kb on + strand at 1154.273 kb on - strand at 1154.292 kb on - strand at 1154.521 kb on + strand, within BBR_RS15090 at 1154.706 kb on + strand, within BBR_RS15090 at 1156.507 kb on + strand at 1156.507 kb on + strand at 1156.515 kb on - strand at 1156.515 kb on - strand at 1156.542 kb on + strand at 1156.550 kb on - strand at 1156.576 kb on + strand at 1156.676 kb on - strand at 1156.701 kb on - strand at 1156.709 kb on - strand at 1156.797 kb on - strand at 1156.812 kb on - strand at 1156.816 kb on - strand at 1156.822 kb on - strand at 1156.890 kb on + strand, within BBR_RS15100 at 1156.902 kb on + strand, within BBR_RS15100 at 1156.969 kb on - strand, within BBR_RS15100 at 1156.974 kb on + strand, within BBR_RS15100 at 1156.974 kb on + strand, within BBR_RS15100 at 1156.986 kb on + strand, within BBR_RS15100 at 1156.987 kb on - strand, within BBR_RS15100 at 1157.050 kb on - strand, within BBR_RS15100 at 1157.097 kb on + strand, within BBR_RS15100 at 1157.097 kb on + strand, within BBR_RS15100 at 1157.199 kb on + strand, within BBR_RS15100 at 1157.216 kb on + strand, within BBR_RS15100 at 1157.224 kb on - strand, within BBR_RS15100 at 1157.237 kb on + strand, within BBR_RS15100 at 1157.237 kb on - strand, within BBR_RS15100 at 1157.251 kb on + strand, within BBR_RS15100 at 1157.310 kb on + strand, within BBR_RS15100 at 1157.323 kb on - strand, within BBR_RS15100 at 1157.341 kb on + strand, within BBR_RS15100 at 1157.345 kb on + strand, within BBR_RS15100 at 1157.353 kb on - strand, within BBR_RS15100 at 1157.397 kb on - strand, within BBR_RS15100 at 1157.409 kb on - strand, within BBR_RS15100 at 1157.425 kb on - strand, within BBR_RS15100 at 1157.439 kb on - strand, within BBR_RS15100 at 1157.508 kb on + strand, within BBR_RS15100 at 1157.518 kb on - strand, within BBR_RS15100
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Cyanocobalamin remove 1,154,191 + +1.8 1,154,193 + +1.3 1,154,201 - +1.8 1,154,219 - -0.8 1,154,228 - +0.0 1,154,229 - -0.7 1,154,239 + +0.6 1,154,273 - -2.3 1,154,292 - -0.8 1,154,521 + BBR_RS15090 0.46 -2.4 1,154,706 + BBR_RS15090 0.87 -1.0 1,156,507 + +0.5 1,156,507 + +1.6 1,156,515 - +0.6 1,156,515 - +0.9 1,156,542 + +1.2 1,156,550 - -0.4 1,156,576 + +0.9 1,156,676 - +1.4 1,156,701 - +0.2 1,156,709 - +0.6 1,156,797 - +1.5 1,156,812 - +0.6 1,156,816 - +0.6 1,156,822 - +0.1 1,156,890 + BBR_RS15100 0.11 -0.1 1,156,902 + BBR_RS15100 0.12 +1.4 1,156,969 - BBR_RS15100 0.16 +0.6 1,156,974 + BBR_RS15100 0.16 +1.0 1,156,974 + BBR_RS15100 0.16 +1.9 1,156,986 + BBR_RS15100 0.17 +0.8 1,156,987 - BBR_RS15100 0.17 +0.4 1,157,050 - BBR_RS15100 0.21 +1.1 1,157,097 + BBR_RS15100 0.24 +2.7 1,157,097 + BBR_RS15100 0.24 +1.4 1,157,199 + BBR_RS15100 0.30 -0.5 1,157,216 + BBR_RS15100 0.31 +1.0 1,157,224 - BBR_RS15100 0.32 +1.1 1,157,237 + BBR_RS15100 0.33 -0.5 1,157,237 - BBR_RS15100 0.33 +2.0 1,157,251 + BBR_RS15100 0.33 +1.8 1,157,310 + BBR_RS15100 0.37 +0.6 1,157,323 - BBR_RS15100 0.38 +1.7 1,157,341 + BBR_RS15100 0.39 +1.3 1,157,345 + BBR_RS15100 0.39 +0.6 1,157,353 - BBR_RS15100 0.40 -0.0 1,157,397 - BBR_RS15100 0.42 -0.4 1,157,409 - BBR_RS15100 0.43 -1.2 1,157,425 - BBR_RS15100 0.44 -0.3 1,157,439 - BBR_RS15100 0.45 +0.0 1,157,508 + BBR_RS15100 0.49 +1.9 1,157,518 - BBR_RS15100 0.50 -1.0
Or see this region's nucleotide sequence