Experiment: CDM1 without Cyanocobalamin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12165 and BBR_RS12170 are separated by 254 nucleotides BBR_RS12170 and BBR_RS12175 are separated by 27 nucleotides BBR_RS12175 and BBR_RS12180 are separated by 71 nucleotides
BBR_RS12165: BBR_RS12165 - ATP-binding protein, at 478,785 to 480,008
_RS12165
BBR_RS12170: BBR_RS12170 - IS256 family transposase, at 480,263 to 480,628
_RS12170
BBR_RS12175: BBR_RS12175 - TetR/AcrR family transcriptional regulator, at 480,656 to 481,321
_RS12175
BBR_RS12180: BBR_RS12180 - DUF2974 domain-containing protein, at 481,393 to 482,730
_RS12180
Position (kb) 480
481
482 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 479.700 kb on - strand, within BBR_RS12165 at 479.707 kb on + strand, within BBR_RS12165 at 479.742 kb on - strand, within BBR_RS12165 at 479.754 kb on + strand, within BBR_RS12165 at 479.767 kb on + strand, within BBR_RS12165 at 479.780 kb on - strand, within BBR_RS12165 at 479.794 kb on + strand, within BBR_RS12165 at 479.838 kb on + strand, within BBR_RS12165 at 479.890 kb on + strand at 479.924 kb on - strand at 479.926 kb on + strand at 479.936 kb on - strand at 479.943 kb on - strand at 479.950 kb on - strand at 479.960 kb on - strand at 479.960 kb on - strand at 480.113 kb on + strand at 480.155 kb on - strand at 480.159 kb on + strand at 480.159 kb on - strand at 480.159 kb on - strand at 480.162 kb on - strand at 480.162 kb on - strand at 480.167 kb on - strand at 480.173 kb on + strand at 480.194 kb on - strand at 480.261 kb on + strand at 480.274 kb on - strand at 480.281 kb on + strand at 480.281 kb on + strand at 480.289 kb on - strand at 480.292 kb on + strand at 480.292 kb on + strand at 480.299 kb on - strand at 480.301 kb on + strand, within BBR_RS12170 at 480.319 kb on - strand, within BBR_RS12170 at 480.357 kb on + strand, within BBR_RS12170 at 480.357 kb on - strand, within BBR_RS12170 at 480.359 kb on - strand, within BBR_RS12170 at 480.359 kb on - strand, within BBR_RS12170 at 480.398 kb on - strand, within BBR_RS12170 at 480.470 kb on - strand, within BBR_RS12170 at 480.483 kb on - strand, within BBR_RS12170 at 480.504 kb on - strand, within BBR_RS12170 at 480.504 kb on - strand, within BBR_RS12170 at 480.512 kb on + strand, within BBR_RS12170 at 480.552 kb on - strand, within BBR_RS12170 at 480.554 kb on + strand, within BBR_RS12170 at 480.602 kb on + strand at 480.616 kb on - strand at 480.635 kb on - strand at 480.643 kb on - strand at 480.647 kb on + strand at 480.650 kb on - strand at 480.650 kb on - strand at 480.654 kb on + strand at 480.654 kb on + strand at 480.654 kb on + strand at 480.654 kb on + strand at 480.654 kb on + strand at 480.660 kb on - strand at 480.662 kb on - strand at 480.678 kb on + strand at 480.689 kb on + strand at 480.703 kb on + strand at 480.711 kb on + strand at 480.723 kb on - strand, within BBR_RS12175 at 480.723 kb on - strand, within BBR_RS12175 at 480.732 kb on - strand, within BBR_RS12175 at 480.770 kb on - strand, within BBR_RS12175 at 480.786 kb on + strand, within BBR_RS12175 at 480.789 kb on - strand, within BBR_RS12175 at 480.789 kb on - strand, within BBR_RS12175 at 480.794 kb on - strand, within BBR_RS12175 at 480.794 kb on - strand, within BBR_RS12175 at 480.794 kb on - strand, within BBR_RS12175 at 480.821 kb on - strand, within BBR_RS12175 at 480.823 kb on + strand, within BBR_RS12175 at 480.823 kb on + strand, within BBR_RS12175 at 480.823 kb on + strand, within BBR_RS12175 at 480.824 kb on - strand, within BBR_RS12175 at 480.831 kb on - strand, within BBR_RS12175 at 480.836 kb on - strand, within BBR_RS12175 at 480.885 kb on + strand, within BBR_RS12175 at 480.885 kb on + strand, within BBR_RS12175 at 480.886 kb on - strand, within BBR_RS12175 at 480.920 kb on - strand, within BBR_RS12175 at 480.957 kb on + strand, within BBR_RS12175 at 480.965 kb on - strand, within BBR_RS12175 at 480.965 kb on - strand, within BBR_RS12175 at 480.975 kb on - strand, within BBR_RS12175 at 480.975 kb on - strand, within BBR_RS12175 at 480.975 kb on - strand, within BBR_RS12175 at 480.975 kb on - strand, within BBR_RS12175 at 480.975 kb on - strand, within BBR_RS12175 at 480.975 kb on - strand, within BBR_RS12175 at 480.975 kb on - strand, within BBR_RS12175 at 480.983 kb on - strand, within BBR_RS12175 at 481.024 kb on + strand, within BBR_RS12175 at 481.053 kb on - strand, within BBR_RS12175 at 481.095 kb on - strand, within BBR_RS12175 at 481.095 kb on - strand, within BBR_RS12175 at 481.095 kb on - strand, within BBR_RS12175 at 481.095 kb on - strand, within BBR_RS12175 at 481.113 kb on + strand, within BBR_RS12175 at 481.252 kb on - strand, within BBR_RS12175 at 481.257 kb on - strand at 481.329 kb on + strand at 481.386 kb on - strand at 481.436 kb on + strand at 481.450 kb on - strand at 481.465 kb on + strand at 481.475 kb on + strand at 481.475 kb on + strand at 481.475 kb on + strand at 481.475 kb on + strand at 481.475 kb on + strand at 481.483 kb on - strand at 481.483 kb on - strand at 481.483 kb on - strand at 481.504 kb on - strand at 481.525 kb on - strand at 481.536 kb on - strand, within BBR_RS12180 at 481.536 kb on - strand, within BBR_RS12180 at 481.575 kb on - strand, within BBR_RS12180 at 481.579 kb on - strand, within BBR_RS12180 at 481.579 kb on - strand, within BBR_RS12180 at 481.590 kb on + strand, within BBR_RS12180 at 481.598 kb on - strand, within BBR_RS12180 at 481.598 kb on - strand, within BBR_RS12180 at 481.598 kb on - strand, within BBR_RS12180 at 481.598 kb on - strand, within BBR_RS12180 at 481.598 kb on - strand, within BBR_RS12180 at 481.599 kb on + strand, within BBR_RS12180 at 481.630 kb on + strand, within BBR_RS12180 at 481.646 kb on + strand, within BBR_RS12180 at 481.649 kb on + strand, within BBR_RS12180 at 481.654 kb on - strand, within BBR_RS12180 at 481.654 kb on - strand, within BBR_RS12180 at 481.663 kb on + strand, within BBR_RS12180 at 481.663 kb on + strand, within BBR_RS12180 at 481.671 kb on + strand, within BBR_RS12180 at 481.671 kb on - strand, within BBR_RS12180 at 481.672 kb on + strand, within BBR_RS12180 at 481.672 kb on + strand, within BBR_RS12180 at 481.672 kb on - strand, within BBR_RS12180 at 481.685 kb on + strand, within BBR_RS12180 at 481.685 kb on + strand, within BBR_RS12180 at 481.685 kb on - strand, within BBR_RS12180 at 481.693 kb on + strand, within BBR_RS12180 at 481.693 kb on - strand, within BBR_RS12180 at 481.695 kb on + strand, within BBR_RS12180 at 481.695 kb on + strand, within BBR_RS12180 at 481.695 kb on + strand, within BBR_RS12180 at 481.703 kb on - strand, within BBR_RS12180 at 481.703 kb on - strand, within BBR_RS12180 at 481.723 kb on + strand, within BBR_RS12180 at 481.741 kb on - strand, within BBR_RS12180 at 481.743 kb on + strand, within BBR_RS12180 at 481.743 kb on + strand, within BBR_RS12180 at 481.743 kb on + strand, within BBR_RS12180 at 481.751 kb on - strand, within BBR_RS12180 at 481.751 kb on - strand, within BBR_RS12180 at 481.751 kb on - strand, within BBR_RS12180 at 481.751 kb on - strand, within BBR_RS12180 at 481.780 kb on - strand, within BBR_RS12180 at 481.780 kb on - strand, within BBR_RS12180 at 481.813 kb on + strand, within BBR_RS12180 at 481.818 kb on + strand, within BBR_RS12180 at 481.818 kb on + strand, within BBR_RS12180 at 481.821 kb on - strand, within BBR_RS12180 at 481.826 kb on - strand, within BBR_RS12180 at 481.826 kb on - strand, within BBR_RS12180 at 481.826 kb on - strand, within BBR_RS12180 at 481.831 kb on - strand, within BBR_RS12180 at 481.841 kb on - strand, within BBR_RS12180 at 481.857 kb on + strand, within BBR_RS12180 at 481.857 kb on + strand, within BBR_RS12180 at 481.857 kb on + strand, within BBR_RS12180 at 481.857 kb on + strand, within BBR_RS12180 at 481.857 kb on + strand, within BBR_RS12180 at 481.857 kb on + strand, within BBR_RS12180 at 481.857 kb on + strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.865 kb on - strand, within BBR_RS12180 at 481.922 kb on - strand, within BBR_RS12180 at 481.935 kb on + strand, within BBR_RS12180 at 481.965 kb on + strand, within BBR_RS12180 at 481.973 kb on - strand, within BBR_RS12180 at 481.973 kb on - strand, within BBR_RS12180 at 481.995 kb on - strand, within BBR_RS12180 at 482.010 kb on - strand, within BBR_RS12180 at 482.010 kb on - strand, within BBR_RS12180 at 482.054 kb on + strand, within BBR_RS12180 at 482.062 kb on - strand, within BBR_RS12180 at 482.076 kb on + strand, within BBR_RS12180 at 482.112 kb on + strand, within BBR_RS12180 at 482.120 kb on - strand, within BBR_RS12180 at 482.120 kb on - strand, within BBR_RS12180 at 482.120 kb on - strand, within BBR_RS12180 at 482.139 kb on + strand, within BBR_RS12180 at 482.139 kb on + strand, within BBR_RS12180 at 482.139 kb on + strand, within BBR_RS12180 at 482.139 kb on + strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.169 kb on + strand, within BBR_RS12180 at 482.170 kb on - strand, within BBR_RS12180 at 482.202 kb on + strand, within BBR_RS12180 at 482.202 kb on + strand, within BBR_RS12180 at 482.214 kb on + strand, within BBR_RS12180 at 482.232 kb on + strand, within BBR_RS12180 at 482.237 kb on + strand, within BBR_RS12180 at 482.240 kb on - strand, within BBR_RS12180 at 482.246 kb on - strand, within BBR_RS12180 at 482.255 kb on - strand, within BBR_RS12180 at 482.303 kb on + strand, within BBR_RS12180 at 482.305 kb on + strand, within BBR_RS12180
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Cyanocobalamin remove 479,700 - BBR_RS12165 0.75 -0.1 479,707 + BBR_RS12165 0.75 -1.0 479,742 - BBR_RS12165 0.78 -2.7 479,754 + BBR_RS12165 0.79 -1.0 479,767 + BBR_RS12165 0.80 +0.6 479,780 - BBR_RS12165 0.81 -0.3 479,794 + BBR_RS12165 0.82 -0.0 479,838 + BBR_RS12165 0.86 -0.7 479,890 + +0.0 479,924 - +0.7 479,926 + -3.0 479,936 - -1.3 479,943 - -1.1 479,950 - -0.6 479,960 - -1.4 479,960 - +0.1 480,113 + -0.0 480,155 - -0.7 480,159 + -0.2 480,159 - -0.9 480,159 - -0.9 480,162 - -1.2 480,162 - -0.7 480,167 - -0.2 480,173 + -0.5 480,194 - -1.1 480,261 + -1.0 480,274 - -1.0 480,281 + -2.3 480,281 + -1.0 480,289 - -0.4 480,292 + -2.3 480,292 + +0.4 480,299 - -0.5 480,301 + BBR_RS12170 0.10 -0.1 480,319 - BBR_RS12170 0.15 -0.8 480,357 + BBR_RS12170 0.26 -1.1 480,357 - BBR_RS12170 0.26 -2.3 480,359 - BBR_RS12170 0.26 -2.2 480,359 - BBR_RS12170 0.26 -0.0 480,398 - BBR_RS12170 0.37 -0.4 480,470 - BBR_RS12170 0.57 -0.4 480,483 - BBR_RS12170 0.60 +0.2 480,504 - BBR_RS12170 0.66 -0.5 480,504 - BBR_RS12170 0.66 +0.2 480,512 + BBR_RS12170 0.68 +0.3 480,552 - BBR_RS12170 0.79 -0.4 480,554 + BBR_RS12170 0.80 +0.3 480,602 + -1.7 480,616 - +0.1 480,635 - -0.2 480,643 - +0.5 480,647 + -2.3 480,650 - -2.5 480,650 - -0.9 480,654 + +0.2 480,654 + +1.5 480,654 + -0.1 480,654 + -2.8 480,654 + -1.1 480,660 - -1.3 480,662 - -1.6 480,678 + +0.8 480,689 + -0.3 480,703 + -2.1 480,711 + -2.8 480,723 - BBR_RS12175 0.10 -0.8 480,723 - BBR_RS12175 0.10 -1.8 480,732 - BBR_RS12175 0.11 -1.9 480,770 - BBR_RS12175 0.17 -1.4 480,786 + BBR_RS12175 0.20 -2.4 480,789 - BBR_RS12175 0.20 -4.0 480,789 - BBR_RS12175 0.20 -1.1 480,794 - BBR_RS12175 0.21 -2.5 480,794 - BBR_RS12175 0.21 -1.7 480,794 - BBR_RS12175 0.21 -0.5 480,821 - BBR_RS12175 0.25 -1.0 480,823 + BBR_RS12175 0.25 -1.8 480,823 + BBR_RS12175 0.25 -2.3 480,823 + BBR_RS12175 0.25 -1.9 480,824 - BBR_RS12175 0.25 -0.8 480,831 - BBR_RS12175 0.26 -0.8 480,836 - BBR_RS12175 0.27 -1.6 480,885 + BBR_RS12175 0.34 -3.1 480,885 + BBR_RS12175 0.34 -3.6 480,886 - BBR_RS12175 0.35 -1.2 480,920 - BBR_RS12175 0.40 -0.3 480,957 + BBR_RS12175 0.45 -3.2 480,965 - BBR_RS12175 0.46 -3.4 480,965 - BBR_RS12175 0.46 -0.1 480,975 - BBR_RS12175 0.48 -1.0 480,975 - BBR_RS12175 0.48 -2.4 480,975 - BBR_RS12175 0.48 -1.4 480,975 - BBR_RS12175 0.48 -3.0 480,975 - BBR_RS12175 0.48 -4.2 480,975 - BBR_RS12175 0.48 -1.3 480,975 - BBR_RS12175 0.48 -1.8 480,983 - BBR_RS12175 0.49 -1.3 481,024 + BBR_RS12175 0.55 -4.3 481,053 - BBR_RS12175 0.60 -2.1 481,095 - BBR_RS12175 0.66 -3.0 481,095 - BBR_RS12175 0.66 -1.4 481,095 - BBR_RS12175 0.66 +1.3 481,095 - BBR_RS12175 0.66 -1.1 481,113 + BBR_RS12175 0.69 -1.9 481,252 - BBR_RS12175 0.89 -3.4 481,257 - -0.9 481,329 + -0.5 481,386 - -1.0 481,436 + +2.3 481,450 - +0.2 481,465 + +0.1 481,475 + +0.7 481,475 + -0.1 481,475 + -0.0 481,475 + +0.7 481,475 + +1.5 481,483 - +0.2 481,483 - +0.3 481,483 - +0.7 481,504 - +0.2 481,525 - -0.1 481,536 - BBR_RS12180 0.11 +0.9 481,536 - BBR_RS12180 0.11 +0.6 481,575 - BBR_RS12180 0.14 +1.7 481,579 - BBR_RS12180 0.14 +1.0 481,579 - BBR_RS12180 0.14 +0.7 481,590 + BBR_RS12180 0.15 +1.6 481,598 - BBR_RS12180 0.15 -0.2 481,598 - BBR_RS12180 0.15 +1.4 481,598 - BBR_RS12180 0.15 +0.3 481,598 - BBR_RS12180 0.15 +1.7 481,598 - BBR_RS12180 0.15 +1.1 481,599 + BBR_RS12180 0.15 +0.5 481,630 + BBR_RS12180 0.18 +1.3 481,646 + BBR_RS12180 0.19 +0.4 481,649 + BBR_RS12180 0.19 +1.9 481,654 - BBR_RS12180 0.20 +0.8 481,654 - BBR_RS12180 0.20 +1.0 481,663 + BBR_RS12180 0.20 +0.6 481,663 + BBR_RS12180 0.20 -0.2 481,671 + BBR_RS12180 0.21 +0.1 481,671 - BBR_RS12180 0.21 +1.2 481,672 + BBR_RS12180 0.21 -0.2 481,672 + BBR_RS12180 0.21 +1.6 481,672 - BBR_RS12180 0.21 +0.6 481,685 + BBR_RS12180 0.22 -0.0 481,685 + BBR_RS12180 0.22 +1.3 481,685 - BBR_RS12180 0.22 +1.2 481,693 + BBR_RS12180 0.22 +0.3 481,693 - BBR_RS12180 0.22 +0.5 481,695 + BBR_RS12180 0.23 -0.6 481,695 + BBR_RS12180 0.23 +1.5 481,695 + BBR_RS12180 0.23 +0.1 481,703 - BBR_RS12180 0.23 +0.5 481,703 - BBR_RS12180 0.23 +1.5 481,723 + BBR_RS12180 0.25 +0.6 481,741 - BBR_RS12180 0.26 +1.8 481,743 + BBR_RS12180 0.26 +0.4 481,743 + BBR_RS12180 0.26 +1.7 481,743 + BBR_RS12180 0.26 +0.6 481,751 - BBR_RS12180 0.27 +0.7 481,751 - BBR_RS12180 0.27 +0.1 481,751 - BBR_RS12180 0.27 +1.5 481,751 - BBR_RS12180 0.27 +2.5 481,780 - BBR_RS12180 0.29 +0.9 481,780 - BBR_RS12180 0.29 +1.0 481,813 + BBR_RS12180 0.31 -0.3 481,818 + BBR_RS12180 0.32 +0.9 481,818 + BBR_RS12180 0.32 +1.8 481,821 - BBR_RS12180 0.32 +1.0 481,826 - BBR_RS12180 0.32 +0.3 481,826 - BBR_RS12180 0.32 +1.2 481,826 - BBR_RS12180 0.32 +2.3 481,831 - BBR_RS12180 0.33 +1.3 481,841 - BBR_RS12180 0.33 -0.6 481,857 + BBR_RS12180 0.35 +1.0 481,857 + BBR_RS12180 0.35 +1.3 481,857 + BBR_RS12180 0.35 +0.0 481,857 + BBR_RS12180 0.35 +0.4 481,857 + BBR_RS12180 0.35 +0.4 481,857 + BBR_RS12180 0.35 +0.6 481,857 + BBR_RS12180 0.35 +0.3 481,865 - BBR_RS12180 0.35 +1.4 481,865 - BBR_RS12180 0.35 +0.8 481,865 - BBR_RS12180 0.35 +0.2 481,865 - BBR_RS12180 0.35 -0.1 481,865 - BBR_RS12180 0.35 +1.3 481,865 - BBR_RS12180 0.35 -0.6 481,865 - BBR_RS12180 0.35 +0.1 481,865 - BBR_RS12180 0.35 +0.6 481,865 - BBR_RS12180 0.35 +0.4 481,865 - BBR_RS12180 0.35 +0.3 481,922 - BBR_RS12180 0.40 +0.9 481,935 + BBR_RS12180 0.41 +1.7 481,965 + BBR_RS12180 0.43 +1.2 481,973 - BBR_RS12180 0.43 +0.6 481,973 - BBR_RS12180 0.43 +1.0 481,995 - BBR_RS12180 0.45 +0.5 482,010 - BBR_RS12180 0.46 +0.6 482,010 - BBR_RS12180 0.46 +0.8 482,054 + BBR_RS12180 0.49 +1.1 482,062 - BBR_RS12180 0.50 +0.2 482,076 + BBR_RS12180 0.51 +1.4 482,112 + BBR_RS12180 0.54 +1.4 482,120 - BBR_RS12180 0.54 +0.7 482,120 - BBR_RS12180 0.54 -0.1 482,120 - BBR_RS12180 0.54 +0.1 482,139 + BBR_RS12180 0.56 +0.2 482,139 + BBR_RS12180 0.56 +1.3 482,139 + BBR_RS12180 0.56 +0.1 482,139 + BBR_RS12180 0.56 +0.5 482,147 - BBR_RS12180 0.56 -0.0 482,147 - BBR_RS12180 0.56 +0.5 482,147 - BBR_RS12180 0.56 +0.1 482,147 - BBR_RS12180 0.56 -0.2 482,147 - BBR_RS12180 0.56 -0.2 482,169 + BBR_RS12180 0.58 +1.3 482,170 - BBR_RS12180 0.58 +0.5 482,202 + BBR_RS12180 0.60 +0.6 482,202 + BBR_RS12180 0.60 +1.2 482,214 + BBR_RS12180 0.61 +0.7 482,232 + BBR_RS12180 0.63 +0.5 482,237 + BBR_RS12180 0.63 +0.5 482,240 - BBR_RS12180 0.63 -0.6 482,246 - BBR_RS12180 0.64 +0.3 482,255 - BBR_RS12180 0.64 -0.4 482,303 + BBR_RS12180 0.68 +0.1 482,305 + BBR_RS12180 0.68 +0.3
Or see this region's nucleotide sequence