Experiment: Fusidic 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS18030 and HSERO_RS18035 are separated by 160 nucleotides HSERO_RS18035 and HSERO_RS18040 are separated by 29 nucleotides HSERO_RS18040 and HSERO_RS18045 are separated by 318 nucleotides HSERO_RS18045 and HSERO_RS18050 are separated by 240 nucleotides
HSERO_RS18030: HSERO_RS18030 - response regulator SirA, at 4,144,537 to 4,144,773
_RS18030
HSERO_RS18035: HSERO_RS18035 - ADP-ribose pyrophosphatase, at 4,144,934 to 4,145,488
_RS18035
HSERO_RS18040: HSERO_RS18040 - alanyl-tRNA synthetase, at 4,145,518 to 4,148,142
_RS18040
HSERO_RS18045: HSERO_RS18045 - hypothetical protein, at 4,148,461 to 4,148,676
_RS18045
HSERO_RS18050: HSERO_RS18050 - glycerophosphodiester phosphodiesterase, at 4,148,917 to 4,149,660
_RS18050
Position (kb) 4145
4146
4147
4148
4149 Strain fitness (log2 ratio) -2
-1
0
1 at 4144.835 kb on - strand at 4144.864 kb on + strand at 4144.871 kb on - strand at 4144.872 kb on - strand at 4145.078 kb on - strand, within HSERO_RS18035 at 4145.133 kb on - strand, within HSERO_RS18035 at 4145.271 kb on + strand, within HSERO_RS18035 at 4145.276 kb on + strand, within HSERO_RS18035 at 4145.291 kb on + strand, within HSERO_RS18035 at 4145.451 kb on - strand at 4148.246 kb on + strand at 4148.254 kb on + strand at 4148.254 kb on + strand at 4148.381 kb on - strand at 4148.383 kb on + strand at 4148.404 kb on + strand at 4148.406 kb on + strand at 4148.412 kb on - strand at 4148.414 kb on - strand at 4148.498 kb on + strand, within HSERO_RS18045 at 4148.717 kb on + strand at 4148.741 kb on + strand at 4148.923 kb on - strand at 4148.962 kb on - strand at 4149.006 kb on + strand, within HSERO_RS18050 at 4149.035 kb on - strand, within HSERO_RS18050
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.025 mg/ml remove 4,144,835 - -1.3 4,144,864 + -0.4 4,144,871 - -1.5 4,144,872 - -0.4 4,145,078 - HSERO_RS18035 0.26 +0.7 4,145,133 - HSERO_RS18035 0.36 +0.9 4,145,271 + HSERO_RS18035 0.61 -2.7 4,145,276 + HSERO_RS18035 0.62 +0.3 4,145,291 + HSERO_RS18035 0.64 -1.9 4,145,451 - -0.8 4,148,246 + -0.2 4,148,254 + -0.6 4,148,254 + -0.0 4,148,381 - -0.4 4,148,383 + -0.7 4,148,404 + -0.5 4,148,406 + -0.5 4,148,412 - +0.3 4,148,414 - +0.7 4,148,498 + HSERO_RS18045 0.17 -1.9 4,148,717 + +0.6 4,148,741 + -2.0 4,148,923 - -1.9 4,148,962 - -0.7 4,149,006 + HSERO_RS18050 0.12 +0.1 4,149,035 - HSERO_RS18050 0.16 -1.2
Or see this region's nucleotide sequence