Experiment: Fusidic 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS12550 and HSERO_RS12555 are separated by 338 nucleotides HSERO_RS12555 and HSERO_RS12560 are separated by 100 nucleotides HSERO_RS12560 and HSERO_RS12565 are separated by 3 nucleotides
HSERO_RS12550: HSERO_RS12550 - D-lactate dehydrogenase, at 2,861,955 to 2,862,947
_RS12550
HSERO_RS12555: HSERO_RS12555 - major facilitator transporter, at 2,863,286 to 2,864,572
_RS12555
HSERO_RS12560: HSERO_RS12560 - antitoxin, at 2,864,673 to 2,865,074
_RS12560
HSERO_RS12565: HSERO_RS12565 - hypothetical protein, at 2,865,078 to 2,865,380
_RS12565
Position (kb)
2863
2864
2865 Strain fitness (log2 ratio)
-2
-1
0
1 at 2862.319 kb on + strand, within HSERO_RS12550 at 2862.445 kb on - strand, within HSERO_RS12550 at 2862.534 kb on + strand, within HSERO_RS12550 at 2862.726 kb on + strand, within HSERO_RS12550 at 2862.827 kb on + strand, within HSERO_RS12550 at 2862.886 kb on + strand at 2862.911 kb on + strand at 2863.066 kb on - strand at 2863.082 kb on + strand at 2863.130 kb on - strand at 2863.260 kb on + strand at 2863.302 kb on - strand at 2863.325 kb on + strand at 2863.325 kb on + strand at 2863.325 kb on + strand at 2863.346 kb on - strand at 2863.450 kb on - strand at 2863.494 kb on + strand, within HSERO_RS12555 at 2863.494 kb on + strand, within HSERO_RS12555 at 2863.559 kb on - strand, within HSERO_RS12555 at 2863.595 kb on - strand, within HSERO_RS12555 at 2863.679 kb on - strand, within HSERO_RS12555 at 2864.004 kb on + strand, within HSERO_RS12555 at 2864.121 kb on + strand, within HSERO_RS12555 at 2864.165 kb on + strand at 2864.173 kb on - strand, within HSERO_RS12555 at 2864.434 kb on - strand, within HSERO_RS12555 at 2864.496 kb on + strand at 2864.661 kb on - strand at 2864.661 kb on - strand at 2864.727 kb on - strand, within HSERO_RS12560 at 2864.822 kb on + strand, within HSERO_RS12560 at 2864.844 kb on + strand at 2864.859 kb on + strand, within HSERO_RS12560 at 2864.962 kb on + strand, within HSERO_RS12560 at 2864.962 kb on + strand, within HSERO_RS12560 at 2864.970 kb on - strand, within HSERO_RS12560 at 2865.021 kb on - strand, within HSERO_RS12560 at 2865.086 kb on + strand at 2865.119 kb on + strand, within HSERO_RS12565 at 2865.133 kb on - strand, within HSERO_RS12565 at 2865.138 kb on - strand, within HSERO_RS12565 at 2865.393 kb on - strand at 2865.424 kb on - strand at 2865.507 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.025 mg/ml remove 2,862,319 + HSERO_RS12550 0.37 +0.4 2,862,445 - HSERO_RS12550 0.49 -0.2 2,862,534 + HSERO_RS12550 0.58 +0.8 2,862,726 + HSERO_RS12550 0.78 -0.5 2,862,827 + HSERO_RS12550 0.88 +0.0 2,862,886 + +0.2 2,862,911 + +0.6 2,863,066 - -1.5 2,863,082 + +0.8 2,863,130 - +1.4 2,863,260 + +0.2 2,863,302 - +0.1 2,863,325 + +0.7 2,863,325 + +0.2 2,863,325 + -1.5 2,863,346 - +1.0 2,863,450 - -0.8 2,863,494 + HSERO_RS12555 0.16 -2.7 2,863,494 + HSERO_RS12555 0.16 +1.0 2,863,559 - HSERO_RS12555 0.21 +0.7 2,863,595 - HSERO_RS12555 0.24 +0.2 2,863,679 - HSERO_RS12555 0.31 +1.4 2,864,004 + HSERO_RS12555 0.56 -0.1 2,864,121 + HSERO_RS12555 0.65 +0.6 2,864,165 + -1.2 2,864,173 - HSERO_RS12555 0.69 -0.1 2,864,434 - HSERO_RS12555 0.89 +0.4 2,864,496 + -0.8 2,864,661 - -1.6 2,864,661 - +0.9 2,864,727 - HSERO_RS12560 0.13 -0.8 2,864,822 + HSERO_RS12560 0.37 -0.5 2,864,844 + -0.2 2,864,859 + HSERO_RS12560 0.46 -0.8 2,864,962 + HSERO_RS12560 0.72 -1.2 2,864,962 + HSERO_RS12560 0.72 -0.1 2,864,970 - HSERO_RS12560 0.74 -2.4 2,865,021 - HSERO_RS12560 0.87 +0.0 2,865,086 + +0.3 2,865,119 + HSERO_RS12565 0.14 +1.0 2,865,133 - HSERO_RS12565 0.18 -2.2 2,865,138 - HSERO_RS12565 0.20 -0.0 2,865,393 - -0.2 2,865,424 - -0.1 2,865,507 - +1.4
Or see this region's nucleotide sequence