Experiment: Fusidic 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS02130 and HSERO_RS02135 are separated by 2 nucleotides HSERO_RS02135 and HSERO_RS02140 are separated by 1 nucleotides HSERO_RS02140 and HSERO_RS02145 are separated by 188 nucleotides HSERO_RS02145 and HSERO_RS02150 are separated by 236 nucleotides
HSERO_RS02130: HSERO_RS02130 - thiazole synthase, at 464,921 to 465,730
_RS02130
HSERO_RS02135: HSERO_RS02135 - phosphomethylpyrimidine kinase, at 465,733 to 466,599
_RS02135
HSERO_RS02140: HSERO_RS02140 - thiamine-phosphate synthase, at 466,601 to 467,233
_RS02140
HSERO_RS02145: HSERO_RS02145 - ferritin, at 467,422 to 467,916
_RS02145
HSERO_RS02150: HSERO_RS02150 - MFS transporter, at 468,153 to 469,328
_RS02150
Position (kb) 466
467
468 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 465.821 kb on - strand, within HSERO_RS02135 at 465.869 kb on - strand, within HSERO_RS02135 at 465.885 kb on - strand, within HSERO_RS02135 at 465.895 kb on - strand, within HSERO_RS02135 at 465.925 kb on - strand, within HSERO_RS02135 at 465.953 kb on - strand, within HSERO_RS02135 at 466.006 kb on - strand, within HSERO_RS02135 at 466.197 kb on + strand, within HSERO_RS02135 at 466.263 kb on + strand, within HSERO_RS02135 at 466.474 kb on - strand, within HSERO_RS02135 at 466.485 kb on - strand at 466.487 kb on + strand, within HSERO_RS02135 at 466.567 kb on + strand at 466.682 kb on + strand, within HSERO_RS02140 at 466.803 kb on - strand at 467.172 kb on + strand at 467.210 kb on - strand at 467.264 kb on + strand at 467.264 kb on + strand at 467.264 kb on + strand at 467.348 kb on - strand at 467.808 kb on + strand, within HSERO_RS02145 at 467.923 kb on + strand at 467.931 kb on - strand at 467.953 kb on + strand at 467.955 kb on + strand at 467.961 kb on - strand at 467.963 kb on - strand at 467.963 kb on - strand at 467.963 kb on - strand at 468.104 kb on + strand at 468.151 kb on + strand at 468.170 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.025 mg/ml remove 465,821 - HSERO_RS02135 0.10 -0.4 465,869 - HSERO_RS02135 0.16 -0.3 465,885 - HSERO_RS02135 0.18 +2.1 465,895 - HSERO_RS02135 0.19 -0.2 465,925 - HSERO_RS02135 0.22 +0.4 465,953 - HSERO_RS02135 0.25 -0.2 466,006 - HSERO_RS02135 0.31 +1.5 466,197 + HSERO_RS02135 0.54 -0.6 466,263 + HSERO_RS02135 0.61 -0.8 466,474 - HSERO_RS02135 0.85 -0.9 466,485 - +2.2 466,487 + HSERO_RS02135 0.87 -0.6 466,567 + -0.1 466,682 + HSERO_RS02140 0.13 -1.3 466,803 - +1.4 467,172 + -0.6 467,210 - +0.0 467,264 + -1.1 467,264 + -1.4 467,264 + -0.1 467,348 - -1.7 467,808 + HSERO_RS02145 0.78 -4.8 467,923 + -2.1 467,931 - +0.1 467,953 + -3.1 467,955 + -2.6 467,961 - -1.4 467,963 - -2.6 467,963 - +0.6 467,963 - -2.4 468,104 + -3.3 468,151 + -0.4 468,170 + -0.8
Or see this region's nucleotide sequence