Experiment: Fusidic 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01850 and HSERO_RS01855 are separated by 322 nucleotides HSERO_RS01855 and HSERO_RS01860 are separated by 19 nucleotides HSERO_RS01860 and HSERO_RS01865 are separated by 1 nucleotides
HSERO_RS01850: HSERO_RS01850 - signal recognition particle protein, at 406,226 to 407,596
_RS01850
HSERO_RS01855: HSERO_RS01855 - myristoyl transferase, at 407,919 to 408,878
_RS01855
HSERO_RS01860: HSERO_RS01860 - sulfonate ABC transporter permease, at 408,898 to 409,719
_RS01860
HSERO_RS01865: HSERO_RS01865 - sulfonate ABC transporter ATP-binding protein, at 409,721 to 410,530
_RS01865
Position (kb) 407
408
409 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 407.685 kb on + strand at 407.718 kb on - strand at 407.728 kb on - strand at 407.728 kb on - strand at 407.753 kb on - strand at 407.901 kb on + strand at 407.909 kb on - strand at 407.938 kb on + strand at 408.038 kb on + strand at 408.154 kb on - strand, within HSERO_RS01855 at 408.462 kb on + strand, within HSERO_RS01855 at 408.504 kb on + strand, within HSERO_RS01855 at 408.634 kb on + strand, within HSERO_RS01855 at 408.642 kb on - strand, within HSERO_RS01855 at 408.869 kb on - strand at 408.875 kb on - strand at 408.886 kb on + strand at 408.963 kb on + strand at 408.980 kb on - strand at 409.056 kb on + strand, within HSERO_RS01860 at 409.064 kb on - strand, within HSERO_RS01860 at 409.064 kb on - strand, within HSERO_RS01860 at 409.205 kb on + strand, within HSERO_RS01860 at 409.311 kb on - strand at 409.326 kb on + strand, within HSERO_RS01860 at 409.493 kb on - strand, within HSERO_RS01860 at 409.493 kb on - strand, within HSERO_RS01860 at 409.495 kb on - strand, within HSERO_RS01860 at 409.495 kb on - strand, within HSERO_RS01860 at 409.569 kb on - strand, within HSERO_RS01860 at 409.749 kb on + strand at 409.749 kb on + strand at 409.757 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.025 mg/ml remove 407,685 + -0.2 407,718 - +0.0 407,728 - -0.8 407,728 - -1.6 407,753 - +0.8 407,901 + -1.4 407,909 - -0.4 407,938 + +0.0 408,038 + +1.8 408,154 - HSERO_RS01855 0.24 +0.3 408,462 + HSERO_RS01855 0.57 -0.9 408,504 + HSERO_RS01855 0.61 -0.8 408,634 + HSERO_RS01855 0.74 -0.0 408,642 - HSERO_RS01855 0.75 -1.1 408,869 - -0.6 408,875 - +0.2 408,886 + -3.0 408,963 + -0.3 408,980 - -0.6 409,056 + HSERO_RS01860 0.19 -1.4 409,064 - HSERO_RS01860 0.20 -0.1 409,064 - HSERO_RS01860 0.20 -1.2 409,205 + HSERO_RS01860 0.37 +0.5 409,311 - +2.9 409,326 + HSERO_RS01860 0.52 -3.0 409,493 - HSERO_RS01860 0.72 -1.0 409,493 - HSERO_RS01860 0.72 +0.2 409,495 - HSERO_RS01860 0.73 -0.6 409,495 - HSERO_RS01860 0.73 +0.3 409,569 - HSERO_RS01860 0.82 -0.3 409,749 + +0.1 409,749 + +0.1 409,757 - -0.3
Or see this region's nucleotide sequence