Experiment: Fusidic 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01115 and HSERO_RS01120 are separated by 115 nucleotides HSERO_RS01120 and HSERO_RS01125 are separated by 28 nucleotides HSERO_RS01125 and HSERO_RS01130 are separated by 81 nucleotides HSERO_RS01130 and HSERO_RS01135 overlap by 4 nucleotides
HSERO_RS01115: HSERO_RS01115 - integrase, at 233,271 to 235,010
_RS01115
HSERO_RS01120: HSERO_RS01120 - hypothetical protein, at 235,126 to 236,010
_RS01120
HSERO_RS01125: HSERO_RS01125 - bacteriophage transcription regulator protein, at 236,039 to 236,407
_RS01125
HSERO_RS01130: HSERO_RS01130 - hypothetical protein, at 236,489 to 236,929
_RS01130
HSERO_RS01135: HSERO_RS01135 - hypothetical protein, at 236,926 to 237,348
_RS01135
Position (kb) 235
236
237 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 234.204 kb on - strand, within HSERO_RS01115 at 234.624 kb on - strand, within HSERO_RS01115 at 234.627 kb on + strand, within HSERO_RS01115 at 234.635 kb on - strand, within HSERO_RS01115 at 234.905 kb on + strand at 235.095 kb on - strand at 235.153 kb on - strand at 235.237 kb on + strand, within HSERO_RS01120 at 235.240 kb on - strand, within HSERO_RS01120 at 235.388 kb on + strand, within HSERO_RS01120 at 235.433 kb on - strand at 235.541 kb on + strand, within HSERO_RS01120 at 235.860 kb on + strand, within HSERO_RS01120 at 235.860 kb on + strand, within HSERO_RS01120 at 235.945 kb on + strand at 235.945 kb on + strand at 236.033 kb on - strand at 236.138 kb on - strand, within HSERO_RS01125 at 236.213 kb on - strand, within HSERO_RS01125 at 236.301 kb on + strand at 236.327 kb on - strand, within HSERO_RS01125 at 236.404 kb on - strand at 236.410 kb on - strand at 236.540 kb on + strand, within HSERO_RS01130 at 236.600 kb on - strand, within HSERO_RS01130 at 236.633 kb on + strand, within HSERO_RS01130 at 236.633 kb on + strand, within HSERO_RS01130 at 236.695 kb on + strand, within HSERO_RS01130 at 236.811 kb on + strand, within HSERO_RS01130 at 236.819 kb on - strand at 236.828 kb on - strand, within HSERO_RS01130 at 236.990 kb on - strand, within HSERO_RS01135 at 236.990 kb on - strand, within HSERO_RS01135
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.025 mg/ml remove 234,204 - HSERO_RS01115 0.54 -0.2 234,624 - HSERO_RS01115 0.78 -2.2 234,627 + HSERO_RS01115 0.78 +1.0 234,635 - HSERO_RS01115 0.78 +3.2 234,905 + -0.5 235,095 - +0.6 235,153 - -0.5 235,237 + HSERO_RS01120 0.13 -1.1 235,240 - HSERO_RS01120 0.13 -0.7 235,388 + HSERO_RS01120 0.30 -0.8 235,433 - -0.8 235,541 + HSERO_RS01120 0.47 -0.0 235,860 + HSERO_RS01120 0.83 -0.7 235,860 + HSERO_RS01120 0.83 -1.5 235,945 + +0.3 235,945 + +0.1 236,033 - +0.2 236,138 - HSERO_RS01125 0.27 +0.6 236,213 - HSERO_RS01125 0.47 +0.0 236,301 + -0.5 236,327 - HSERO_RS01125 0.78 -0.3 236,404 - +0.0 236,410 - +1.5 236,540 + HSERO_RS01130 0.12 -0.3 236,600 - HSERO_RS01130 0.25 -0.0 236,633 + HSERO_RS01130 0.33 -1.5 236,633 + HSERO_RS01130 0.33 -1.3 236,695 + HSERO_RS01130 0.47 +0.6 236,811 + HSERO_RS01130 0.73 -0.3 236,819 - -0.1 236,828 - HSERO_RS01130 0.77 -0.4 236,990 - HSERO_RS01135 0.15 -0.2 236,990 - HSERO_RS01135 0.15 +1.3
Or see this region's nucleotide sequence